Restriction map for
pFN21AE5694
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Aor51HI ApaI ApoI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbrPI BbvCI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
DraI DraIII Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI HpaI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MseI MunI
NcoI NdeI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Alw44I 1144 GTGCAC G^TGCAC
AlwI 734 GGATC GGATC(4/5)
ApaLI 1144 GTGCAC G^TGCAC
AscI 237 GGCGCGCC GG^CGCGCC
Asp700I 439 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 107 TGCGCA TGC^GCA
BalI 779 TGGCCA TGG^CCA
BcgI 170 CGAGGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 821 TGATCA T^GATCA
BcnI 190 CCCGG CC^[GC]GG
BglI 15 GCCGGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsrBI 681 CCGCTC CCGCTC(-3/-3)
BssSI 34 CTCGTG CACGAG(-5/-1)
DsaI 720 CCACGG C^C[GA][CT]GG
EcoNI 1298 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 821 TGATCA T^GATCA
FspI 107 TGCGCA TGC^GCA
HindIII 882 AAGCTT A^AGCTT
MluNI 779 TGGCCA TGG^CCA
MscI 779 TGGCCA TGG^CCA
NaeI 234 GCCGGC GCC^GGC
NciI 190 CCCGG CC^[GC]GG
NgoMIV 234 GCCGGC G^CCGGC
NheI 273 GCTAGC G^CTAGC
NotI 491 GCGGCCGC GC^GGCCGC
NspI 725 GCATGC [GA]CATG^[CT]
PflMI 669 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 704 GGGACCT [GA]G^G[AT]CC[CT]
PstI 742 CTGCAG CTGCA^G
SacI 1335 GAGCTC GAGCT^C
SphI 725 GCATGC GCATG^C
StuI 615 AGGCCT AGG^CCT
TfiI 1313 GAATC G^A[AT]TC
Van91I 669 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 787 CCAGGGGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 439 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 296 GTCGAC GT^[AC][GT]AC
815 GTCGAC
AcyI 54 GGCGCC G[GA]^CG[CT]C
345 GGCGCC
AocI 1187 CCTCAGG CC^T[ACGT]AGG
1272 CCTAAGG
AspHI 1144 GTGCAC G[AT]GC[AT]^C
1335 GAGCTC
BbeI 54 GGCGCC GGCGC^C
345 GGCGCC
BbsI 949 GAAGAC GAAGAC(2/6)
1571 GTCTTC
BpuAI 949 GAAGAC GAAGAC(2/6)
1571 GTCTTC
BsaHI 54 GGCGCC G[GA]^CG[CT]C
345 GGCGCC
BsiHKAI 1144 GTGCAC G[AT]GC[AT]^C
1335 GAGCTC
BsmAI 1110 GAGAC GTCTC(1/5)
1343 GAGAC
Bsu36I 1187 CCTCAGG CC^T[ACGT]AGG
1272 CCTAAGG
CpoI 74 CGGTCCG CG^G[AT]CCG
192 CGGACCG
DpnII 735 GATC ^GATC
822 GATC
DrdI 299 GACGCGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
818 GACTGATCAGTC
Eco81I 1187 CCTCAGG CC^T[ACGT]AGG
1272 CCTAAGG
EcoT14I 674 CCTTGG C^C[AT][AT]GG
686 CCTTGG
HaeII 54 GGCGCC [GA]GCGC^[CT]
345 GGCGCC
HgaI 299 GACGC GACGC(5/10)
306 GCGTC
Hin1I 54 GGCGCC G[GA]^CG[CT]C
345 GGCGCC
HincII 296 GTCGAC GT[CT]^[GA]AC
815 GTCGAC
HindII 296 GTCGAC GT[CT]^[GA]AC
815 GTCGAC
KasI 54 GGCGCC G^GCGCC
345 GGCGCC
MboI 735 GATC ^GATC
822 GATC
NarI 54 GGCGCC GG^CGCC
345 GGCGCC
NdeII 735 GATC ^GATC
822 GATC
PvuII 745 CAGCTG CAG^CTG
938 CAGCTG
RsrII 74 CGGTCCG CG^G[AT]CCG
192 CGGACCG
SalI 296 GTCGAC G^TCGAC
815 GTCGAC
SapI 915 GAAGAGC GCTCTTC(1/4)
1332 GAAGAGC
Sau3AI 735 GATC ^GATC
822 GATC
SfoI 54 GGCGCC GGC^GCC
345 GGCGCC
SmlI 281 CTCAAG C^T[CT][GA]AG
1153 CTCAAG
StyI 674 CCTTGG C^C[AT][AT]GG
686 CCTTGG
Tsp509I 641 AATT ^AATT
1411 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaI
AvaII BanI BanII BbvI BfaI
BmyI BpmI BsaJI BsgI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I