EIF2D ORF clone pFN21AE5610

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5610

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Clone info.

Species

human

Clone Name

pFN21AE5610 Sequence (with vector) Restriction Map

Product ID

FHC08010

ORF Sequence

DNA sequence (1752 bp)
Protein sequence (584 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5610

Symbol

EIF2D

Alias : HCA56, LGTN

Description

eukaryotic translation initiation factor 2D, transcript variant 1

Original Clone ID

oc95610

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1752 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 584 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1465.1 0 100.0 EIF2D CCDS55680.1 8.8e-107 78.8 EIF2D The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001950 490 568 PF01253 Translation initiation factor SUI1 HMMSmart IPR002478 99 175 SM00359 Pseudouridine synthase/archaeosine transglycosylase ProfileScan IPR002478 93 173 PS50890 Pseudouridine synthase/archaeosine transglycosylase IPR001950 491 564 PS50296 Translation initiation factor SUI1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_008824 584 613709 100.0 100.0 XP_011507559 581 613709 98.1 98.1 XP_005277566 534 613709 91.4 100.0 NP_001188407 460 613709 78.8 100.0 XP_016856019 424 613709 72.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5610 Download

>KIEE5610  1752 bp
ATGTTTGCCAAGGCCTTTCGGGTCAAGTCCAACACGGCCATCAAGGGGTCGGACAGGAGA
AAGCTTCGAGCTGATGTGACAACTGCTTTCCCCACCCTTGGAACTGATCAAGTCTCTGAG
TTAGTACCTGGAAAGGAGGAGCTCAACATTGTGAAGTTGTATGCTCACAAAGGGGATGCA
GTGACTGTGTACGTGAGTGGTGGTAACCCCATCCTCTTTGAACTGGAGAAAAATCTGTAT
CCAACAGTGTACACGCTGTGGTCCTATCCTGATCTTCTGCCAACCTTTACAACATGGCCT
CTGGTGCTCGAGAAACTGGTAGGGGGAGCAGATTTGATGCTGCCTGGACTGGTGATGCCC
CCTGCTGGTCTGCCTCAGGTACAGAAGGGCGACCTCTGTGCCATTTCTTTGGTGGGGAAC
AGAGCCCCTGTAGCCATTGGAGTTGCAGCCATGTCCACAGCTGAGATGCTCACGTCAGGC
CTGAAGGGAAGGGGCTTCTCTGTGCTCCACACTTACCAGGACCACTTGTGGCGGTCTGGA
AACAAGTCCTCTCCACCTTCCATTGCTCCACTGGCCCTGGATTCAGCAGATCTCAGTGAA
GAGAAGGGGTCTGTCCAGATGGACTCCACCCTGCAGGGAGACATGAGGCACATGACCCTG
GAGGGGGAAGAGGAGAATGGGGAGGTTCACCAGGCACGTGAAGACAAGTCTCTCTCAGAA
GCCCCAGAAGACACCAGCACCAGGGGCCTGAACCAAGACTCCACAGATAGCAAAACGCTT
CAAGAACAAATGGATGAGCTGTTACAGCAATGCTTCTTACATGCCTTGAAGTGCCGAGTC
AAAAAGGCTGACCTCCCTTTACTCACCAGCACTTTCCTTGGCAGCCACATGTTCTCCTGC
TGCCCCGAAGGACGACAACTGGACATAAAGAAGTCAAGCTACAAAAAGCTCTCTAAGTTC
CTGCAGCAAATGCAGCAGGAGCAGATTATACAGGTGAAGGAGCTGAGCAAAGGGGTGGAG
AGCATTGTGGCTGTGGACTGGAAACACCCGAGGATTACATCTTTCGTCATACCCGAGCCC
TCCCCGACCTCCCAGACTATCCAGGAGGGTAGCAGGGAACAGCCCTATCACCCTCCAGAT
ATAAAACCCCTCTACTGTGTCCCAGCCAGCATGACCCTGCTCTTCCAGGAGTCTGGCCAC
AAGAAGGGGAGCTTTCTGGAGGGCAGTGAGGTCCGAACGATCGTCATTAACTACGCCAAG
AAAAATGACCTGGTTGATGCAGACAACAAAAATCTTGTGAGATTGGATCCCATCCTATGT
GACTGCATCTTAGAGAAAAATGAACAGCATACAGTCATGAAGCTTCCATGGGACAGTCTT
CTGACCAGGTGTTTGGAAAAATTACAGCCTGCCTATCAAGTGACCCTTCCCGGACAAGAG
CCCATTGTGAAGAAAGGGAGAATCTGTCCAATTGACATCACCCTAGCACAAAGAGCGTCT
AATAAAAAGGTGACCGTGGTCCGGAACTTGGAGGCCTATGGTCTGGACCCATACTCAGTG
GCTGCCATCCTTCAGCAGCGATGCCAGGCTAGCACCACCGTCAATCCTGCCCCTGGGGCC
AAGGACAGCCTTCAGGTGCAGATCCAGGGAAACCAGGTCCACCACCTCGGCTGGCTATTG
CTTGAAGAGTATCAGCTCCCTCGAAAACACATCCAAGGTCTAGAAAAGGCCCTCAAACCT
GGCAAGAAGAAG

ORF Protein

Cloned ORF protein sequence for pFN21AE5610 Download

>KIEE5610  584 aa
MFAKAFRVKSNTAIKGSDRRKLRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDA
VTVYVSGGNPILFELEKNLYPTVYTLWSYPDLLPTFTTWPLVLEKLVGGADLMLPGLVMP
PAGLPQVQKGDLCAISLVGNRAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDHLWRSG
NKSSPPSIAPLALDSADLSEEKGSVQMDSTLQGDMRHMTLEGEEENGEVHQAREDKSLSE
APEDTSTRGLNQDSTDSKTLQEQMDELLQQCFLHALKCRVKKADLPLLTSTFLGSHMFSC
CPEGRQLDIKKSSYKKLSKFLQQMQQEQIIQVKELSKGVESIVAVDWKHPRITSFVIPEP
SPTSQTIQEGSREQPYHPPDIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAK
KNDLVDADNKNLVRLDPILCDCILEKNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQE
PIVKKGRICPIDITLAQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGA
KDSLQVQIQGNQVHHLGWLLLEEYQLPRKHIQGLEKALKPGKKK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5610 Download

>pFN21AE5610  6457 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTTGCCAAGGCCTTTCGGGTCAAGTCCAACAC
GGCCATCAAGGGGTCGGACAGGAGAAAGCTTCGAGCTGATGTGACAACTGCTTTCCCCAC
CCTTGGAACTGATCAAGTCTCTGAGTTAGTACCTGGAAAGGAGGAGCTCAACATTGTGAA
GTTGTATGCTCACAAAGGGGATGCAGTGACTGTGTACGTGAGTGGTGGTAACCCCATCCT
CTTTGAACTGGAGAAAAATCTGTATCCAACAGTGTACACGCTGTGGTCCTATCCTGATCT
TCTGCCAACCTTTACAACATGGCCTCTGGTGCTCGAGAAACTGGTAGGGGGAGCAGATTT
GATGCTGCCTGGACTGGTGATGCCCCCTGCTGGTCTGCCTCAGGTACAGAAGGGCGACCT
CTGTGCCATTTCTTTGGTGGGGAACAGAGCCCCTGTAGCCATTGGAGTTGCAGCCATGTC
CACAGCTGAGATGCTCACGTCAGGCCTGAAGGGAAGGGGCTTCTCTGTGCTCCACACTTA
CCAGGACCACTTGTGGCGGTCTGGAAACAAGTCCTCTCCACCTTCCATTGCTCCACTGGC
CCTGGATTCAGCAGATCTCAGTGAAGAGAAGGGGTCTGTCCAGATGGACTCCACCCTGCA
GGGAGACATGAGGCACATGACCCTGGAGGGGGAAGAGGAGAATGGGGAGGTTCACCAGGC
ACGTGAAGACAAGTCTCTCTCAGAAGCCCCAGAAGACACCAGCACCAGGGGCCTGAACCA
AGACTCCACAGATAGCAAAACGCTTCAAGAACAAATGGATGAGCTGTTACAGCAATGCTT
CTTACATGCCTTGAAGTGCCGAGTCAAAAAGGCTGACCTCCCTTTACTCACCAGCACTTT
CCTTGGCAGCCACATGTTCTCCTGCTGCCCCGAAGGACGACAACTGGACATAAAGAAGTC
AAGCTACAAAAAGCTCTCTAAGTTCCTGCAGCAAATGCAGCAGGAGCAGATTATACAGGT
GAAGGAGCTGAGCAAAGGGGTGGAGAGCATTGTGGCTGTGGACTGGAAACACCCGAGGAT
TACATCTTTCGTCATACCCGAGCCCTCCCCGACCTCCCAGACTATCCAGGAGGGTAGCAG
GGAACAGCCCTATCACCCTCCAGATATAAAACCCCTCTACTGTGTCCCAGCCAGCATGAC
CCTGCTCTTCCAGGAGTCTGGCCACAAGAAGGGGAGCTTTCTGGAGGGCAGTGAGGTCCG
AACGATCGTCATTAACTACGCCAAGAAAAATGACCTGGTTGATGCAGACAACAAAAATCT
TGTGAGATTGGATCCCATCCTATGTGACTGCATCTTAGAGAAAAATGAACAGCATACAGT
CATGAAGCTTCCATGGGACAGTCTTCTGACCAGGTGTTTGGAAAAATTACAGCCTGCCTA
TCAAGTGACCCTTCCCGGACAAGAGCCCATTGTGAAGAAAGGGAGAATCTGTCCAATTGA
CATCACCCTAGCACAAAGAGCGTCTAATAAAAAGGTGACCGTGGTCCGGAACTTGGAGGC
CTATGGTCTGGACCCATACTCAGTGGCTGCCATCCTTCAGCAGCGATGCCAGGCTAGCAC
CACCGTCAATCCTGCCCCTGGGGCCAAGGACAGCCTTCAGGTGCAGATCCAGGGAAACCA
GGTCCACCACCTCGGCTGGCTATTGCTTGAAGAGTATCAGCTCCCTCGAAAACACATCCA
AGGTCTAGAAAAGGCCCTCAAACCTGGCAAGAAGAAGGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT