################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbvCI BfrI BlnI BsaBI BsaHI BsaI BsiWI BsmBI BsmI BspDI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MluI MslI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tth111I Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 1520 TCCGGA T^CCGGA AciI 531 GCGG CCGC(-3/-1) AflIII 887 ACATGT A^C[GA][CT]GT AocI 373 CCTCAGG CC^T[ACGT]AGG BalI 1194 TGGCCA TGG^CCA BamHI 1305 GGATCC G^GATCC BbrPI 695 CACGTG CAC^GTG BcgI 390 CGACCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 237 GTATCC GTATCC(6/5) BclI 105 TGATCA T^GATCA BcnI 1429 CCCGG CC^[GC]GG BglI 1609 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 588 AGATCT A^GATCT BlpI 1002 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1002 GCTGAGC GC^T[ACGT]AGC BsaWI 1520 TCCGGA [AT]^CCGG[AT] BseAI 1520 TCCGGA T^CCGGA BsgI 1636 GTGCAG GTGCAG(16/14) BsiEI 1238 CGATCG CG[GA][CT]^CG Bsp1407I 248 TGTACA T^GTACA BspEI 1520 TCCGGA T^CCGGA BspHI 1355 TCATGA T^CATGA BspLU11I 887 ACATGT A^CATGT BsrGI 248 TGTACA T^GTACA BstAPI 963 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 373 CCTCAGG CC^T[ACGT]AGG CelII 1002 GCTGAGC GC^T[ACGT]AGC DraIII 252 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 373 CCTCAGG CC^T[ACGT]AGG FbaI 105 TGATCA T^GATCA HgaI 1495 GCGTC GACGC(5/10) MfeI 1469 CAATTG C^AATTG MluNI 1194 TGGCCA TGG^CCA MroI 1520 TCCGGA T^CCGGA MscI 1194 TGGCCA TGG^CCA MseI 1247 TTAA T^TAA MspA1I 458 CAGCTG C[AC]G^C[GT]G MunI 1469 CAATTG C^AATTG NciI 1429 CCCGG CC^[GC]GG NcoI 1366 CCATGG C^CATGG NheI 1588 GCTAGC G^CTAGC PaeR7I 307 CTCGAG C^TCGAG PmaCI 695 CACGTG CAC^GTG PmlI 695 CACGTG CAC^GTG PshAI 1512 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1238 CGATCG CGAT^CG PvuII 458 CAGCTG CAG^CTG RcaI 1355 TCATGA T^CATGA SacI 139 GAGCTC GAGCT^C SapI 1179 GCTCTTC GCTCTTC(1/4) SbfI 630 CCTGCAGG CCTGCA^GG SmlI 307 CTCGAG C^T[CT][GA]AG Sse8387I 630 CCTGCAGG CCTGCA^GG SspBI 248 TGTACA T^GTACA Tru9I 1247 TTAA T^TAA XbaI 1719 TCTAGA T^CTAGA XhoI 307 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 179 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 1556 CAGTGGCTG BsaAI 190 TACGTG [CT]AC^GT[GA] 695 CACGTG BseRI 136 GAGGAG GAGGAG(10/8) 670 GAGGAG BsmFI 1370 GGGAC GGGAC(10/14) 1159 GTCCC BsrDI 561 CATTGC GCAATG(2/0) 807 GCAATG BstEII 202 GGTAACC G^GT[ACGT]ACC 1509 GGTGACC BstPI 202 GGTAACC G^GT[ACGT]ACC 1509 GGTGACC DraII 743 GGGGCCT [GA]G^G[ACGT]CC[CT] 1727 AGGCCCT DsaI 1366 CCATGG C^C[GA][CT]GG 1514 CCGTGG EaeI 35 CGGCCA [CT]^GGCC[GA] 1194 TGGCCA EcoO109I 743 GGGGCCT [GA]G^G[ACGT]CC[CT] 1727 AGGCCCT EcoO65I 202 GGTAACC G^GT[ACGT]ACC 1509 GGTGACC HapII 1430 CCGG C^CGG 1521 CCGG HindIII 61 AAGCTT A^AGCTT 1360 AAGCTT HpaII 1430 CCGG C^CGG 1521 CCGG MspI 1430 CCGG C^CGG 1521 CCGG NspI 819 ACATGC [GA]CATG^[CT] 887 ACATGT PstI 631 CTGCAG CTGCA^G 961 CTGCAG TfiI 580 GATTC G^A[AT]TC 1460 GAATC Tsp509I 1400 AATT ^AATT 1470 AATT XcmI 401 CCATTTCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 560 CCATTGCTCCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AfaI AluI AlwI AspHI AvaI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII