(Circular) (Six-base) MAPSORT of: pFN21AE5610.seq Check: 840 from: 1 to: 6457 pFN21AE5610 6457 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4267 278 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3800 1937 Size: 1863 4594 Acc65I G'GTAC_C Cuts at: 3778 3778 Size: 6457 AclI AA'CG_TT Cuts at: 1563 4584 4957 1563 Size: 3021 373 3063 Fragments arranged by size: 3063 3021 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 3560 3620 4520 5904 6172 1632 Size: 875 1053 60 900 1384 268 1917 Fragments arranged by size: 1917 1384 1053 900 875 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6260 AflIII A'CryG_T Cuts at: 1203 2892 5356 6333 1203 Size: 1689 2464 977 1327 Fragments arranged by size: 2464 1689 1327 977 AgeI A'CCGG_T Cuts at: 5288 5288 Size: 6457 AhdI GACnn_n'nnGTC Cuts at: 5186 5186 Size: 6457 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3197 1184 Size: 2013 4444 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2747 2779 2747 Size: 32 6425 AlwNI CAG_nnn'CTG Cuts at: 2189 3566 4200 5772 2189 Size: 1377 634 1572 2874 Fragments arranged by size: 2874 1572 1377 634 ApaLI G'TGCA_C Cuts at: 4514 5670 4514 Size: 1156 5301 ApoI r'AATT_y Cuts at: 1493 3766 3992 4144 6253 1493 Size: 2273 226 152 2109 1697 Fragments arranged by size: 2273 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5011 160 Size: 872 3979 1606 Fragments arranged by size: 3979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 AvaI C'yCGr_G Cuts at: 1870 1943 2312 3052 3077 3782 1870 Size: 73 369 740 25 705 4545 Fragments arranged by size: 4545 740 705 369 73 25 BamHI G'GATC_C Cuts at: 3310 3787 4154 3310 Size: 477 367 5613 Fragments arranged by size: 5613 477 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3778 5233 618 Size: 325 217 627 1991 1455 1842 Fragments arranged by size: 1991 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2148 2431 3084 3447 3776 1391 Size: 757 283 653 363 329 4072 Fragments arranged by size: 4072 757 653 363 329 283 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2712 2739 3374 928 Size: 971 813 27 635 4011 Fragments arranged by size: 4011 971 813 635 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2384 2418 4648 4682 1903 Size: 34 447 34 2230 34 3678 Fragments arranged by size: 3678 2230 447 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2253 4349 5559 2253 Size: 2096 1210 3151 Fragments arranged by size: 3151 2096 1210 BclI T'GATC_A Cuts at: 1548 2110 1548 Size: 562 5895 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3620 5068 136 Size: 107 122 71 631 605 1948 1448 1525 Fragments arranged by size: 1948 1525 1448 631 605 122 107 71 BglII A'GATC_T Cuts at: 2593 6452 2593 Size: 3859 2598 BlpI GC'TnA_GC Cuts at: 3008 3874 3008 Size: 866 5591 Bme1580I G_kGCm'C Cuts at: 1790 4518 5674 1790 Size: 2728 1156 2573 Fragments arranged by size: 2728 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2478 1318 Size: 1160 5297 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4188 5146 442 Size: 416 53 83 399 24 2771 958 1753 Fragments arranged by size: 2771 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3597 1056 Size: 2541 3916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2684 3123 3242 5117 1239 Size: 1010 435 439 119 1875 2579 Fragments arranged by size: 2579 1875 1010 439 435 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4582 5447 5745 4582 Size: 865 298 5294 Fragments arranged by size: 5294 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5120 882 Size: 617 3621 2219 Fragments arranged by size: 3621 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2197 2702 4248 493 Size: 711 993 505 1546 2702 Fragments arranged by size: 2702 1546 993 711 505 BsaBI GATnn'nnATC Cuts at: 6451 6451 Size: 6457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4264 4646 5281 275 Size: 53 83 186 936 2731 382 635 1451 Fragments arranged by size: 2731 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3525 4890 5288 5562 5709 6120 3525 Size: 1365 398 274 147 411 3862 Fragments arranged by size: 3862 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2747 2777 2882 2912 3454 Size: 30 165 30 1344 30 105 30 542 Cuts at: 3454 3484 1178 Size: 30 4151 Fragments arranged by size: 4151 1344 542 165 105 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2690 1186 Size: 970 534 4953 Fragments arranged by size: 4953 970 534 BseYI C'CCAG_C Cuts at: 3166 5660 6156 3166 Size: 2494 496 3467 Fragments arranged by size: 3467 2494 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3662 1163 Size: 573 1926 3958 Fragments arranged by size: 3958 1926 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3246 3907 4668 5696 1540 Size: 275 186 1245 661 761 1028 2301 Fragments arranged by size: 2301 1245 1028 761 661 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2313 2511 4518 4603 5674 2148 Size: 165 198 2007 85 1071 2931 Fragments arranged by size: 2931 2007 1071 198 165 85 BsmI GAATG_Cn' Cuts at: 3968 4061 3968 Size: 93 6364 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2313 2431 2511 3084 3447 3776 Size: 399 358 165 118 80 573 363 329 Cuts at: 3776 4518 4603 5674 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 573 399 363 358 329 165 118 85 80 BspEI T'CCGG_A Cuts at: 3525 6120 3525 Size: 2595 3862 BspHI T'CATG_A Cuts at: 3360 4346 6076 3360 Size: 986 1730 3741 Fragments arranged by size: 3741 1730 986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3812 844 Size: 2968 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4344 1394 Size: 429 2521 3507 Fragments arranged by size: 3507 2521 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2565 2819 4952 5126 65 Size: 1183 1317 254 2133 174 1396 Fragments arranged by size: 2133 1396 1317 1183 254 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5101 5288 1700 Size: 110 3291 187 2869 Fragments arranged by size: 3291 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2253 96 Size: 2157 4300 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6457 BssSI C'ACGA_G Cuts at: 4517 5529 4517 Size: 1012 5445 BstAPI GCAn_nnn'nTGC Cuts at: 794 2974 794 Size: 2180 4277 BstBI TT'CG_AA Cuts at: 5261 5261 Size: 6457 BstEII G'GTnAC_C Cuts at: 1184 2207 3514 1184 Size: 1023 1307 4127 Fragments arranged by size: 4127 1307 1023 BstXI CCAn_nnnn'nTGG Cuts at: 2413 3197 3397 2413 Size: 784 200 5473 Fragments arranged by size: 5473 784 200 BstYI r'GATC_y Cuts at: 1972 2593 3310 3645 3787 4154 4539 4556 6008 Size: 621 717 335 142 367 385 17 1452 Cuts at: 6008 6094 6106 6452 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 717 621 385 367 346 335 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2379 2379 Size: 6457 BtgI C'CryG_G Cuts at: 513 1065 3371 3519 513 Size: 552 2306 148 3451 Fragments arranged by size: 3451 2306 552 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3598 487 Size: 683 56 2372 3346 Fragments arranged by size: 3346 2372 683 56 BtsI GCAGTG_nn' Cuts at: 2190 3235 3973 4767 4787 2190 Size: 1045 738 794 20 3860 Fragments arranged by size: 3860 1045 794 738 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6422 DraI TTT'AAA Cuts at: 3761 3761 Size: 6457 DraIII CAC_nnn'GTG Cuts at: 2262 2262 Size: 6457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5464 809 Size: 507 4148 1802 Fragments arranged by size: 4148 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 3199 3904 Size: 54 1425 50 275 18 210 1159 705 Cuts at: 3904 4793 6422 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1159 889 705 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3904 1537 Size: 275 2092 4090 Fragments arranged by size: 4090 2092 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2598 2667 3191 3684 4387 1353 Size: 1245 69 524 493 703 3423 Fragments arranged by size: 3423 1245 703 524 493 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5044 5418 5564 387 Size: 1064 138 3455 374 146 1280 Fragments arranged by size: 3455 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2146 Size: 6457 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2507 2684 3123 3242 3560 3620 Size: 393 617 258 177 439 119 318 60 Cuts at: 3620 4520 5117 5904 6172 1239 Size: 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 617 597 439 393 318 268 258 177 119 60 EcoO109I rG'GnC_Cy Cuts at: 2749 3733 2749 Size: 984 5473 EcoRI G'AATT_C Cuts at: 3766 4144 3766 Size: 378 6079 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3232 3446 3232 Size: 214 6243 FspI TGC'GCA Cuts at: 4963 4963 Size: 6457 HaeII r_GCGC'y Cuts at: 1678 5604 1678 Size: 3926 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2597 2629 3295 Size: 32 135 32 530 32 463 32 666 Cuts at: 3295 3327 3454 3486 3542 3574 5098 5130 5172 Size: 32 127 32 56 32 1524 32 42 Cuts at: 5172 5204 1373 Size: 32 2626 Fragments arranged by size: 2626 1524 666 530 463 135 127 56 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3801 4047 669 Size: 988 281 1863 246 3079 Fragments arranged by size: 3079 1863 988 281 246 HindIII A'AGCT_T Cuts at: 748 2066 3365 748 Size: 1318 1299 3840 Fragments arranged by size: 3840 1318 1299 HpaI GTT'AAC Cuts at: 4047 4047 Size: 6457 KpnI G_GTAC'C Cuts at: 3782 3782 Size: 6457 MfeI C'AATT_G Cuts at: 3474 4056 3474 Size: 582 5875 MluI A'CGCG_T Cuts at: 6333 6333 Size: 6457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 2058 2270 5571 5755 1305 Size: 729 24 212 3301 184 2007 Fragments arranged by size: 3301 2007 729 212 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 3201 10 Size: 54 1425 343 1369 3266 Fragments arranged by size: 3266 1425 1369 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4415 4774 4933 518 Size: 648 3249 359 159 2042 Fragments arranged by size: 3249 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2465 3873 4550 5698 5943 1606 Size: 859 1408 677 1148 245 2120 Fragments arranged by size: 2120 1408 1148 859 677 245 NcoI C'CATG_G Cuts at: 513 1065 3371 513 Size: 552 2306 3599 Fragments arranged by size: 3599 2306 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6457 NheI G'CTAG_C Cuts at: 1052 3593 1052 Size: 2541 3916 NotI GC'GGCC_GC Cuts at: 3904 3904 Size: 6457 NspI r_CATG'y Cuts at: 1132 1229 2828 2896 3815 5360 1132 Size: 97 1599 68 919 1545 2229 Fragments arranged by size: 2229 1599 1545 919 97 68 PasI CC'CwG_GG Cuts at: 1348 3617 1348 Size: 2269 4188 PciI A'CATG_T Cuts at: 2892 5356 2892 Size: 2464 3993 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6457 PfoI T'CCnGG_A Cuts at: 3105 3189 3433 3105 Size: 84 244 6129 Fragments arranged by size: 6129 244 84 PmeI GTTT'AAAC Cuts at: 3761 3761 Size: 6457 PmlI CAC'GTG Cuts at: 2702 2702 Size: 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2747 2779 4526 4558 6039 6071 2747 Size: 32 1747 32 1481 32 3133 Fragments arranged by size: 3133 1747 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3521 1613 Size: 1908 4549 PsiI TTA'TAA Cuts at: 4027 4027 Size: 6457 PstI C_TGCA'G Cuts at: 830 2640 2970 3809 830 Size: 1810 330 839 3478 Fragments arranged by size: 3478 1810 839 330 PvuI CG_AT'CG Cuts at: 2001 3246 2001 Size: 1245 5212 PvuII CAG'CTG Cuts at: 2465 2465 Size: 6457 SacI G_AGCT'C Cuts at: 2148 2148 Size: 6457 SalI G'TCGA_C Cuts at: 1936 3799 1936 Size: 1863 4594 SapI GCTCTTCn'nnn_ Cuts at: 3191 3191 Size: 6457 SbfI CC_TGCA'GG Cuts at: 2640 3809 2640 Size: 1169 5288 ScaI AGT'ACT Cuts at: 4705 6417 4705 Size: 1712 4745 SexAI A'CCwGG_T Cuts at: 3273 3389 3657 3273 Size: 116 268 6073 Fragments arranged by size: 6073 268 116 SfcI C'TryA_G Cuts at: 826 1045 2433 2636 2966 3805 4940 5621 5812 Size: 219 1388 203 330 839 1135 681 191 Cuts at: 5812 826 Size: 1471 Fragments arranged by size: 1471 1388 1135 839 681 330 219 203 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6457 SmaI CCC'GGG Cuts at: 1872 3784 1872 Size: 1912 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 4561 5462 5724 820 Size: 197 926 369 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 369 262 197 SnaBI TAC'GTA Cuts at: 493 4248 493 Size: 3755 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6457 SphI G_CATG'C Cuts at: 1132 3815 1132 Size: 2683 3774 SspI AAT'ATT Cuts at: 5 52 4381 5 Size: 47 4329 2081 Fragments arranged by size: 4329 2081 47 StuI AGG'CCT Cuts at: 1517 2018 2484 3539 1517 Size: 501 466 1055 4435 Fragments arranged by size: 4435 1055 501 466 StyI C'CwwG_G Cuts at: 513 1065 2013 2101 2881 3371 3624 3718 3896 Size: 552 948 88 780 490 253 94 178 Cuts at: 3896 513 Size: 3074 Fragments arranged by size: 3074 948 780 552 490 253 178 94 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4484 4501 4654 4839 5284 325 Size: 1255 2904 17 153 185 445 1498 Fragments arranged by size: 2904 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 4703 6415 Size: 274 80 33 51 1443 276 2450 1712 Cuts at: 6415 96 Size: 138 Fragments arranged by size: 2450 1712 1443 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 2882 2914 3592 3624 6417 6449 2516 Size: 32 334 32 678 32 2793 32 2524 Fragments arranged by size: 2793 2524 678 334 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6457 XbaI T'CTAG_A Cuts at: 3724 3793 3724 Size: 69 6388 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2413 2572 2413 Size: 159 6298 XhoI C'TCGA_G Cuts at: 1943 2312 1943 Size: 369 6088 XmaI C'CCGG_G Cuts at: 1870 3782 1870 Size: 1912 4545 XmnI GAAnn'nnTTC Cuts at: 4586 4586 Size: 6457 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4265 276 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI