(Linear) (Six-base) MAPSORT of: KIEE5610.seq Check: 3836 from: 1 to: 1752 KIEE5610.orf With 182 enzymes: SgfI * December 5, 2014 13:02 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 1555 1615 1752 Size: 502 1053 60 137 Fragments arranged by size: 1053 502 137 60 AflIII A'CryG_T Cuts at: 0 887 1752 Size: 887 865 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1192 1752 Size: 1192 560 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 742 774 1752 Size: 742 32 978 Fragments arranged by size: 978 742 32 AlwNI CAG_nnn'CTG Cuts at: 0 184 1561 1752 Size: 184 1377 191 Fragments arranged by size: 1377 191 184 AvaI C'yCGr_G Cuts at: 0 307 1047 1072 1752 Size: 307 740 25 680 Fragments arranged by size: 740 680 307 25 BamHI G'GATC_C Cuts at: 0 1305 1752 Size: 1305 447 BanII G_rGCy'C Cuts at: 0 143 426 1079 1442 1752 Size: 143 283 653 363 310 Fragments arranged by size: 653 363 310 283 143 BbsI GAAGACnn'nnnn_ Cuts at: 0 707 734 1369 1752 Size: 707 27 635 383 Fragments arranged by size: 707 635 383 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 379 413 1752 Size: 379 34 1339 Fragments arranged by size: 1339 379 34 BciVI GTATCCnnnnn_n' Cuts at: 0 248 1752 Size: 248 1504 BclI T'GATC_A Cuts at: 0 105 1752 Size: 105 1647 BglI GCCn_nnn'nGGC Cuts at: 0 1615 1752 Size: 1615 137 BglII A'GATC_T Cuts at: 0 588 1752 Size: 588 1164 BlpI GC'TnA_GC Cuts at: 0 1003 1752 Size: 1003 749 BmgBI CAC'GTC Cuts at: 0 473 1752 Size: 473 1279 BmtI G_CTAG'C Cuts at: 0 1592 1752 Size: 1592 160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 679 1118 1237 1752 Size: 244 435 439 119 515 Fragments arranged by size: 515 439 435 244 119 BsaAI yAC'GTr Cuts at: 0 192 697 1752 Size: 192 505 1055 Fragments arranged by size: 1055 505 192 BsaWI w'CCGG_w Cuts at: 0 1520 1752 Size: 1520 232 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 742 772 877 907 1449 1479 1752 Size: 742 30 105 30 542 30 273 Fragments arranged by size: 742 542 273 105 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 151 685 1752 Size: 151 534 1067 Fragments arranged by size: 1067 534 151 BseYI C'CCAG_C Cuts at: 0 1161 1752 Size: 1161 591 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1657 1752 Size: 1657 95 BsiEI CG_ry'CG Cuts at: 0 1241 1752 Size: 1241 511 BsiHKAI G_wGCw'C Cuts at: 0 143 308 506 1752 Size: 143 165 198 1246 Fragments arranged by size: 1246 198 165 143 Bsp1286I G_dGCh'C Cuts at: 0 143 308 426 506 1079 1442 1752 Size: 143 165 118 80 573 363 310 Fragments arranged by size: 573 363 310 165 143 118 80 BspEI T'CCGG_A Cuts at: 0 1520 1752 Size: 1520 232 BspHI T'CATG_A Cuts at: 0 1355 1752 Size: 1355 397 BsrDI GCAATG_nn' Cuts at: 0 560 814 1752 Size: 560 254 938 Fragments arranged by size: 938 560 254 BsrGI T'GTAC_A Cuts at: 0 248 1752 Size: 248 1504 BstAPI GCAn_nnn'nTGC Cuts at: 0 969 1752 Size: 969 783 BstEII G'GTnAC_C Cuts at: 0 202 1509 1752 Size: 202 1307 243 Fragments arranged by size: 1307 243 202 BstXI CCAn_nnnn'nTGG Cuts at: 0 408 1192 1392 1752 Size: 408 784 200 360 Fragments arranged by size: 784 408 360 200 BstYI r'GATC_y Cuts at: 0 588 1305 1640 1752 Size: 588 717 335 112 Fragments arranged by size: 717 588 335 112 Bsu36I CC'TnA_GG Cuts at: 0 374 1752 Size: 374 1378 BtgI C'CryG_G Cuts at: 0 1366 1514 1752 Size: 1366 148 238 Fragments arranged by size: 1366 238 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1593 1752 Size: 1593 159 BtsI GCAGTG_nn' Cuts at: 0 185 1230 1752 Size: 185 1045 522 Fragments arranged by size: 1045 522 185 DraIII CAC_nnn'GTG Cuts at: 0 257 1752 Size: 257 1495 EaeI y'GGCC_r Cuts at: 0 35 1194 1752 Size: 35 1159 558 Fragments arranged by size: 1159 558 35 EarI CTCTTCn'nnn_ Cuts at: 0 593 662 1186 1679 1752 Size: 593 69 524 493 73 Fragments arranged by size: 593 524 493 73 69 EcoICRI GAG'CTC Cuts at: 0 141 1752 Size: 141 1611 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 502 679 1118 1237 1555 1615 1752 Size: 244 258 177 439 119 318 60 137 Fragments arranged by size: 439 318 258 244 177 137 119 60 EcoO109I rG'GnC_Cy Cuts at: 0 744 1728 1752 Size: 744 984 24 Fragments arranged by size: 984 744 24 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1227 1441 1752 Size: 1227 214 311 Fragments arranged by size: 1227 311 214 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 97 129 592 624 1290 1322 1449 1481 Size: 97 32 463 32 666 32 127 32 Cuts at: 1481 1537 1569 1752 Size: 56 32 183 Fragments arranged by size: 666 463 183 127 97 56 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 61 1360 1752 Size: 61 1299 392 Fragments arranged by size: 1299 392 61 MfeI C'AATT_G Cuts at: 0 1469 1752 Size: 1469 283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 29 53 265 1752 Size: 29 24 212 1487 Fragments arranged by size: 1487 212 29 24 MscI TGG'CCA Cuts at: 0 1196 1752 Size: 1196 556 MspA1I CmG'CkG Cuts at: 0 460 1752 Size: 460 1292 NcoI C'CATG_G Cuts at: 0 1366 1752 Size: 1366 386 NheI G'CTAG_C Cuts at: 0 1588 1752 Size: 1588 164 NspI r_CATG'y Cuts at: 0 823 891 1752 Size: 823 68 861 Fragments arranged by size: 861 823 68 PasI CC'CwG_GG Cuts at: 0 1612 1752 Size: 1612 140 PciI A'CATG_T Cuts at: 0 887 1752 Size: 887 865 PfoI T'CCnGG_A Cuts at: 0 1100 1184 1428 1752 Size: 1100 84 244 324 Fragments arranged by size: 1100 324 244 84 PmlI CAC'GTG Cuts at: 0 697 1752 Size: 697 1055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 742 774 1752 Size: 742 32 978 Fragments arranged by size: 978 742 32 PshAI GACnn'nnGTC Cuts at: 0 1516 1752 Size: 1516 236 PstI C_TGCA'G Cuts at: 0 635 965 1752 Size: 635 330 787 Fragments arranged by size: 787 635 330 PvuI CG_AT'CG Cuts at: 0 1241 1752 Size: 1241 511 PvuII CAG'CTG Cuts at: 0 460 1752 Size: 460 1292 SacI G_AGCT'C Cuts at: 0 143 1752 Size: 143 1609 SapI GCTCTTCn'nnn_ Cuts at: 0 1186 1752 Size: 1186 566 SbfI CC_TGCA'GG Cuts at: 0 635 1752 Size: 635 1117 SexAI A'CCwGG_T Cuts at: 0 1268 1384 1652 1752 Size: 1268 116 268 100 Fragments arranged by size: 1268 268 116 100 SfcI C'TryA_G Cuts at: 0 428 631 961 1752 Size: 428 203 330 791 Fragments arranged by size: 791 428 330 203 SmlI C'TyrA_G Cuts at: 0 307 1752 Size: 307 1445 StuI AGG'CCT Cuts at: 0 13 479 1534 1752 Size: 13 466 1055 218 Fragments arranged by size: 1055 466 218 13 StyI C'CwwG_G Cuts at: 0 8 96 876 1366 1619 1713 1752 Size: 8 88 780 490 253 94 39 Fragments arranged by size: 780 490 253 94 88 39 8 TatI w'GTAC_w Cuts at: 0 248 1752 Size: 248 1504 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 511 543 877 909 1587 1619 1752 Size: 511 32 334 32 678 32 133 Fragments arranged by size: 678 511 334 133 32 32 32 XbaI T'CTAG_A Cuts at: 0 1719 1752 Size: 1719 33 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 408 567 1752 Size: 408 159 1185 Fragments arranged by size: 1185 408 159 XhoI C'TCGA_G Cuts at: 0 307 1752 Size: 307 1445 Enzymes that do cut: AcuI AflIII AlfI AloI AlwNI AvaI BamHI BanII BbsI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BpmI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrDI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII MfeI MmeI MscI MspA1I NcoI NheI NspI PasI PciI PfoI PmlI PpiI PshAI PstI PvuI PvuII SacI SapI SbfI SexAI SfcI SmlI StuI StyI TatI TstI XbaI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BfrBI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsiWI BsmI BsmBI BspMI BsrBI BsrFI BssHII BssSI BstBI BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PflMI PmeI PpuMI PsiI PspOMI PspXI PsrI RsrII SacII SalI SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XmaI XmnI ZraI