ASTL ORF clone pFN21AE5568

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5568

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Clone info.

Species

human

Clone Name

pFN21AE5568 Sequence (with vector) Restriction Map

Product ID

FHC31636

ORF Sequence

DNA sequence (1293 bp)
Protein sequence (431 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5568

Symbol

ASTL

Alias : SAS1B

Description

astacin-like metallo-endopeptidase (M12 family)

Original Clone ID

oc95568

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1293 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 431 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33249.1 2.5e-103 99.5 ASTL CCDS77173.1 1.2e-11 37.8 MEP1B CCDS45846.1 1.2e-11 37.8 MEP1B CCDS34856.1 5.7e-11 38.1 BMP1 CCDS7449.1 5.7e-11 35.2 TLL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001506 120 138 PR00480 Peptidase M12A IPR001506 174 192 PR00480 Peptidase M12A IPR001506 193 210 PR00480 Peptidase M12A IPR001506 229 244 PR00480 Peptidase M12A IPR001506 267 280 PR00480 Peptidase M12A HMMPfam IPR001506 103 282 PF01400 Peptidase M12A HMMSmart IPR006026 90 234 SM00235 Peptidase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 8 WPWVLGLLSLPGVILGAPLASSC 30 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001002036 431 608860 99.5 100.0 XP_011509507 436 608860 99.3 96.8 XP_011509508 432 608860 98.3 97.4 XP_011509509 418 608860 95.0 96.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5568 Download

>KIEE5568  1293 bp
ATGGAGGGTGTAGGGGGTCTCTGGCCTTGGGTGCTGGGTCTGCTCTCCTTGCCAGGTGTG
ATCCTAGGAGCGCCCCTGGCCTCCAGCTGCGCAGGAGCCTGTGGTACCAGCTTCCCAGAT
GGCCTCACCCCTGAGGGAACCCAGGCCTCCGGGGACAAGGACATTCCTGCAATTAACCAA
GGGCTCATCCTGGAAGAAACCCCAGAGAGCAGCTTCCTCATCGAGGGGGACATCATCCGG
CCGAGTCCCTTCCGACTGCTGTCAGCAACCAGCAACAAATGGCCCATGGGTGGTAGTGGT
GTCGTGGAGGTCCCCTTCCTGCTCTCCAGCAAGTACGATGAGCCCAGCCGCCAGGTCATC
CTGGAGGCTCTTGCGGAGTTTGAACGTTCCACGTGCATCAGGTTTGTCACCTATCAGGAC
CAGAGAGACTTCATTTCCATCATCCCCATGTATGGGTGCTTCTCGAGTGTGGGGCGCAGT
GGAGGGATGCAGGTGGTCTCCCTGGCGCCCACGTGTCTCCAGAAGGGCCGGGGCATTGTC
CTTCATGAGCTCATGCATGTGCTGGGCTTCTGGCACGAGCACACGCGGGCCGACCGGGAC
CGCTATATCCGTGTCAACTGGAACGAGATCCTGCCAGGCTTTGAAATCAACTTCATCAAG
TCTCGGAGCAGCAACATGCTGACGCCCTATGACTACTCCTCTGTGATGCACTATGGGAGG
CTTGCCTTCAGCCGGCGTGGGCTGCCCACCATCACACCACTTTGGGCCCCCAGTGTCCAC
ATCGGCCAGCGATGGAACCTGAGTGCCTCGGACATCACCCGGGTCCTCCAACTCTACGGC
TGCAGCCCAAGTGGCCCCAGGCCCCGTGGGAGAGGGTCCCATGCCCACAGCACTGGTAGG
AGCCCCGCTCCGGCCTCCCTATCTCTGCAGCGGCTTTTGGAGGCACTGTCGGCGGAATCC
AGGAGCCCCGACCCCAGTGGTTCCAGTGCGGGAGGCCAGCCCGTTCCTGCAGGGCCTGGG
GAGAGCCCACATGGGTGGGAGTCCCCTGCCCTGAAAAAGCTCAGTGCAGAGGCCTCGGCA
AGGCAGCCTCAGACCCTGGCTTCCTCCCCAAGATCAAGGCCTGGAGCAGGTGCCCCCGGT
GTTGCTCAGGAGCAGTCCTGGCTGGCCGGAGTGTCCACCAAGCCCACAGTCCCATCTTCA
GAAGCAGGAATCCAGCCAGTCCCTGTCCAGGGAAGCCCAGCTCTGCCAGGGGGCTGTGTA
CCTAGAAATCATTTCAAGGGGATGTCCGAAGAT

ORF Protein

Cloned ORF protein sequence for pFN21AE5568 Download

>KIEE5568  431 aa
MEGVGGLWPWVLGLLSLPGVILGAPLASSCAGACGTSFPDGLTPEGTQASGDKDIPAINQ
GLILEETPESSFLIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVI
LEALAEFERSTCIRFVTYQDQRDFISIIPMYGCFSSVGRSGGMQVVSLAPTCLQKGRGIV
LHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSRSSNMLTPYDYSSVMHYGR
LAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLQLYGCSPSGPRPRGRGSHAHSTGR
SPAPASLSLQRLLEALSAESRSPDPSGSSAGGQPVPAGPGESPHGWESPALKKLSAEASA
RQPQTLASSPRSRPGAGAPGVAQEQSWLAGVSTKPTVPSSEAGIQPVPVQGSPALPGGCV
PRNHFKGMSED

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5568 Download

>pFN21AE5568  5998 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGGGTGTAGGGGGTCTCTGGCCTTGGGTGCT
GGGTCTGCTCTCCTTGCCAGGTGTGATCCTAGGAGCGCCCCTGGCCTCCAGCTGCGCAGG
AGCCTGTGGTACCAGCTTCCCAGATGGCCTCACCCCTGAGGGAACCCAGGCCTCCGGGGA
CAAGGACATTCCTGCAATTAACCAAGGGCTCATCCTGGAAGAAACCCCAGAGAGCAGCTT
CCTCATCGAGGGGGACATCATCCGGCCGAGTCCCTTCCGACTGCTGTCAGCAACCAGCAA
CAAATGGCCCATGGGTGGTAGTGGTGTCGTGGAGGTCCCCTTCCTGCTCTCCAGCAAGTA
CGATGAGCCCAGCCGCCAGGTCATCCTGGAGGCTCTTGCGGAGTTTGAACGTTCCACGTG
CATCAGGTTTGTCACCTATCAGGACCAGAGAGACTTCATTTCCATCATCCCCATGTATGG
GTGCTTCTCGAGTGTGGGGCGCAGTGGAGGGATGCAGGTGGTCTCCCTGGCGCCCACGTG
TCTCCAGAAGGGCCGGGGCATTGTCCTTCATGAGCTCATGCATGTGCTGGGCTTCTGGCA
CGAGCACACGCGGGCCGACCGGGACCGCTATATCCGTGTCAACTGGAACGAGATCCTGCC
AGGCTTTGAAATCAACTTCATCAAGTCTCGGAGCAGCAACATGCTGACGCCCTATGACTA
CTCCTCTGTGATGCACTATGGGAGGCTTGCCTTCAGCCGGCGTGGGCTGCCCACCATCAC
ACCACTTTGGGCCCCCAGTGTCCACATCGGCCAGCGATGGAACCTGAGTGCCTCGGACAT
CACCCGGGTCCTCCAACTCTACGGCTGCAGCCCAAGTGGCCCCAGGCCCCGTGGGAGAGG
GTCCCATGCCCACAGCACTGGTAGGAGCCCCGCTCCGGCCTCCCTATCTCTGCAGCGGCT
TTTGGAGGCACTGTCGGCGGAATCCAGGAGCCCCGACCCCAGTGGTTCCAGTGCGGGAGG
CCAGCCCGTTCCTGCAGGGCCTGGGGAGAGCCCACATGGGTGGGAGTCCCCTGCCCTGAA
AAAGCTCAGTGCAGAGGCCTCGGCAAGGCAGCCTCAGACCCTGGCTTCCTCCCCAAGATC
AAGGCCTGGAGCAGGTGCCCCCGGTGTTGCTCAGGAGCAGTCCTGGCTGGCCGGAGTGTC
CACCAAGCCCACAGTCCCATCTTCAGAAGCAGGAATCCAGCCAGTCCCTGTCCAGGGAAG
CCCAGCTCTGCCAGGGGGCTGTGTACCTAGAAATCATTTCAAGGGGATGTCCGAAGATGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT