(Circular) (Six-base) MAPSORT of: pFN21AE5568.seq Check: 8258 from: 1 to: 5998 pFN21AE5568 5998 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2485 3122 2485 Size: 637 5361 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 2108 3319 2108 Size: 1211 4787 AclI AA'CG_TT Cuts at: 1563 2389 4125 4498 1563 Size: 826 1736 373 3063 Fragments arranged by size: 3063 1736 826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2716 3186 4061 5445 5713 1632 Size: 1084 470 875 1384 268 1917 Fragments arranged by size: 1917 1384 1084 875 470 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 2516 4897 5874 1203 Size: 1313 2381 977 1327 Fragments arranged by size: 2381 1327 1313 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 3037 1166 Size: 1871 4127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2696 1184 Size: 1512 4486 AlwNI CAG_nnn'CTG Cuts at: 3020 3100 3227 3741 5313 3020 Size: 80 127 514 1572 3705 Fragments arranged by size: 3705 1572 514 127 80 ApaI G_GGCC'C Cuts at: 2773 2773 Size: 5998 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 3307 3533 3685 5794 1493 Size: 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2467 2823 3323 1870 Size: 73 524 356 500 4545 Fragments arranged by size: 4545 524 500 356 73 AvrII C'CTAG_G Cuts at: 2068 2068 Size: 5998 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2108 2509 3134 3319 4774 Size: 325 217 627 321 401 625 185 1455 Cuts at: 4774 618 Size: 1842 Fragments arranged by size: 1842 1455 627 625 401 325 321 217 185 BanII G_rGCy'C Cuts at: 1391 2190 2349 2556 2773 2909 2972 3032 3317 Size: 799 159 207 217 136 63 60 285 Cuts at: 3317 1391 Size: 4072 Fragments arranged by size: 4072 799 285 217 207 159 136 63 60 BbeI G_GCGC'C Cuts at: 2513 2513 Size: 5998 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3060 3094 4189 4223 1903 Size: 34 1123 34 1095 34 3678 Fragments arranged by size: 3678 1123 1095 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BfrBI ATG'CAT Cuts at: 2560 2560 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2856 4609 136 Size: 107 122 71 631 605 1184 1753 1525 Fragments arranged by size: 1753 1525 1184 631 605 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2773 3139 4059 5215 1790 Size: 983 366 920 1156 2573 Fragments arranged by size: 2573 1156 983 920 366 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2769 2973 3729 Size: 416 53 83 399 24 1352 204 756 Cuts at: 3729 4687 442 Size: 958 1753 Fragments arranged by size: 1753 1352 958 756 416 399 204 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2928 2928 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2314 2387 2507 3147 4658 1239 Size: 832 243 73 120 640 1511 2579 Fragments arranged by size: 2579 1511 832 640 243 120 73 Bpu10I CC'TnA_GC Cuts at: 3150 3150 Size: 5998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4988 5286 4123 Size: 865 298 4835 Fragments arranged by size: 4835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2027 2506 4661 882 Size: 617 528 479 2155 2219 Fragments arranged by size: 2219 2155 617 528 479 BsaAI yAC'GTr Cuts at: 493 1204 2397 2517 3789 493 Size: 711 1193 120 1272 2702 Fragments arranged by size: 2702 1272 1193 711 120 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2510 2687 3805 4187 Size: 53 83 186 936 977 177 1118 382 Cuts at: 4187 4822 275 Size: 635 1451 Fragments arranged by size: 1451 1118 977 936 635 382 186 177 83 53 BsaWI w'CCGG_w Cuts at: 4431 4829 5103 5250 5661 4431 Size: 398 274 147 411 4768 Fragments arranged by size: 4768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2936 2966 1178 Size: 30 165 30 1533 30 4210 Fragments arranged by size: 4210 1533 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2692 1186 Size: 1506 4492 BseYI C'CCAG_C Cuts at: 2038 2348 2566 3241 5201 5697 2038 Size: 310 218 675 1960 496 2339 Fragments arranged by size: 2339 1960 675 496 310 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3090 1163 Size: 573 1354 4071 Fragments arranged by size: 4071 1354 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2246 2599 3448 4209 5237 1540 Size: 275 186 245 353 849 761 1028 2301 Fragments arranged by size: 2301 1028 849 761 353 275 245 186 BsiHKAI G_wGCw'C Cuts at: 2556 2586 4059 4144 5215 2556 Size: 30 1473 85 1071 3339 Fragments arranged by size: 3339 1473 1071 85 30 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2349 2556 2586 2773 2909 2972 Size: 399 400 159 207 30 187 136 63 Cuts at: 2972 3032 3139 3317 4059 4144 5215 1391 Size: 60 107 178 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 207 187 178 159 136 107 85 63 60 30 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 2548 3887 5617 2548 Size: 1339 1730 2929 Fragments arranged by size: 2929 1730 1339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2485 3122 3353 844 Size: 1641 637 231 3489 Fragments arranged by size: 3489 1641 637 231 BsrBI CCG'CTC Cuts at: 1394 1823 2912 3885 1394 Size: 429 1089 973 3507 Fragments arranged by size: 3507 1089 973 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2736 4642 4829 1700 Size: 110 926 1906 187 2869 Fragments arranged by size: 2869 1906 926 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 2579 4058 5070 2579 Size: 1479 1012 3507 Fragments arranged by size: 3507 1479 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5998 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2897 3039 2897 Size: 142 5856 BstYI r'GATC_y Cuts at: 1972 2631 3328 3695 4080 4097 5549 5635 5647 Size: 659 697 367 385 17 1452 86 12 Cuts at: 5647 5993 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 697 659 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2136 2136 Size: 5998 BtgI C'CryG_G Cuts at: 513 1065 2004 2289 2869 513 Size: 552 939 285 580 3642 Fragments arranged by size: 3642 939 580 552 285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2809 487 Size: 683 56 1583 3676 Fragments arranged by size: 3676 1583 683 56 BtsI GCAGTG_nn' Cuts at: 2488 3514 4308 4328 2488 Size: 1026 794 20 4158 Fragments arranged by size: 4158 1026 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2243 2788 3168 Size: 54 1425 50 275 18 413 545 380 Cuts at: 3168 3445 4334 5963 8 Size: 277 889 1629 43 Fragments arranged by size: 1629 1425 889 545 413 380 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2243 3445 1537 Size: 275 431 1202 4090 Fragments arranged by size: 4090 1202 431 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3928 1353 Size: 2575 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2972 4585 4959 5105 387 Size: 1064 138 1383 1613 374 146 1280 Fragments arranged by size: 1613 1383 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2554 2554 Size: 5998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2314 2387 2507 2716 3147 3186 Size: 393 439 243 73 120 209 431 39 Cuts at: 3186 4061 4658 5445 5713 1239 Size: 875 597 787 268 1524 Fragments arranged by size: 1524 875 787 597 439 431 393 268 243 209 120 73 39 EcoNI CCTnn'n_nnAGG Cuts at: 2225 3082 3129 2225 Size: 857 47 5094 Fragments arranged by size: 5094 857 47 EcoO109I rG'GnC_Cy Cuts at: 2314 2770 2827 2865 2880 3017 2314 Size: 456 57 38 15 137 5295 Fragments arranged by size: 5295 456 137 57 38 15 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FspI TGC'GCA Cuts at: 2095 4504 2095 Size: 2409 3589 HaeII r_GCGC'y Cuts at: 1678 2078 2513 5145 1678 Size: 400 435 2632 2531 Fragments arranged by size: 2632 2531 435 400 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2687 2719 2936 2968 4639 Size: 32 135 32 1115 32 217 32 1671 Cuts at: 4639 4671 4713 4745 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1671 1115 217 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2620 3342 3588 669 Size: 988 281 682 722 246 3079 Fragments arranged by size: 3079 988 722 682 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KasI G'GCGC_C Cuts at: 2509 2509 Size: 5998 KpnI G_GTAC'C Cuts at: 2112 3323 2112 Size: 1211 4787 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2281 2857 5112 5296 1305 Size: 976 576 2255 184 2007 Fragments arranged by size: 2255 2007 976 576 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4176 Fragments arranged by size: 4176 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 2561 3037 3956 4315 4474 518 Size: 648 1284 111 476 919 359 159 2042 Fragments arranged by size: 2042 1284 919 648 476 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2091 2935 3414 4091 5239 5484 1606 Size: 485 844 479 677 1148 245 2120 Fragments arranged by size: 2120 1148 844 677 485 479 245 NaeI GCC'GGC Cuts at: 2738 2738 Size: 5998 NarI GG'CG_CC Cuts at: 2510 2510 Size: 5998 NcoI C'CATG_G Cuts at: 513 1065 2004 2289 513 Size: 552 939 285 4222 Fragments arranged by size: 4222 939 552 285 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NgoMIV G'CCGG_C Cuts at: 2736 2736 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NsiI A_TGCA'T Cuts at: 2562 2562 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2564 2683 3356 4901 1132 Size: 97 1335 119 673 1545 2229 Fragments arranged by size: 2229 1545 1335 673 119 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PfoI T'CCnGG_A Cuts at: 2193 2364 2963 2193 Size: 171 599 5228 Fragments arranged by size: 5228 599 171 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PmlI CAC'GTG Cuts at: 2397 2517 2397 Size: 120 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2314 2827 2880 2314 Size: 513 53 5432 Fragments arranged by size: 5432 513 53 PshAI GACnn'nnGTC Cuts at: 1613 2263 1613 Size: 650 5348 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspOMI G'GGCC_C Cuts at: 2769 2769 Size: 5998 PstI C_TGCA'G Cuts at: 830 2849 2934 3016 3350 830 Size: 2019 85 82 334 3478 Fragments arranged by size: 3478 2019 334 85 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2091 2091 Size: 5998 SacI G_AGCT'C Cuts at: 2556 2556 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SanDI GG'GwC_CC Cuts at: 2880 2880 Size: 5998 SbfI CC_TGCA'GG Cuts at: 3016 3350 3016 Size: 334 5664 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2845 2930 3012 3346 4481 5162 5353 Size: 219 1800 85 82 334 1135 681 191 Cuts at: 5353 826 Size: 1471 Fragments arranged by size: 1800 1471 1135 681 334 219 191 85 82 SfoI GGC'GCC Cuts at: 2511 2511 Size: 5998 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 2825 3325 1872 Size: 953 500 4545 Fragments arranged by size: 4545 953 500 SmlI C'TyrA_G Cuts at: 820 1017 1943 2467 4102 5003 5265 820 Size: 197 926 524 1635 901 262 1553 Fragments arranged by size: 1635 1553 926 901 524 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 2150 3077 3124 1517 Size: 633 927 47 4391 Fragments arranged by size: 4391 927 633 47 StyI C'CwwG_G Cuts at: 513 1065 2004 2030 2068 2182 2289 3437 513 Size: 552 939 26 38 114 107 1148 3074 Fragments arranged by size: 3074 1148 939 552 114 107 38 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2023 2282 2448 3027 4025 4042 4195 Size: 1255 443 259 166 579 998 17 153 Cuts at: 4195 4380 4825 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 998 579 445 443 259 185 166 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4244 5956 96 Size: 274 80 33 51 1443 2267 1712 138 Fragments arranged by size: 2267 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2791 2823 5958 5990 2791 Size: 32 3135 32 2799 Fragments arranged by size: 3135 2799 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 2467 1943 Size: 524 5474 XmaI C'CCGG_G Cuts at: 1870 2823 3323 1870 Size: 953 500 4545 Fragments arranged by size: 4545 953 500 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI