(Linear) (Six-base) MAPSORT of: KIEE5568.seq Check: 7300 from: 1 to: 1293 KIEE5568.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 480 1117 1293 Size: 480 637 176 Fragments arranged by size: 637 480 176 Acc65I G'GTAC_C Cuts at: 0 103 1293 Size: 103 1190 AclI AA'CG_TT Cuts at: 0 384 1293 Size: 384 909 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 711 1181 1293 Size: 711 470 112 Fragments arranged by size: 711 470 112 AflIII A'CryG_T Cuts at: 0 511 1293 Size: 511 782 AleI CACnn'nnGTG Cuts at: 0 1032 1293 Size: 1032 261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 691 1293 Size: 691 602 AlwNI CAG_nnn'CTG Cuts at: 0 1015 1095 1222 1293 Size: 1015 80 127 71 Fragments arranged by size: 1015 127 80 71 ApaI G_GGCC'C Cuts at: 0 768 1293 Size: 768 525 AvaI C'yCGr_G Cuts at: 0 462 818 1293 Size: 462 356 475 Fragments arranged by size: 475 462 356 AvrII C'CTAG_G Cuts at: 0 63 1293 Size: 63 1230 BanI G'GyrC_C Cuts at: 0 103 504 1129 1293 Size: 103 401 625 164 Fragments arranged by size: 625 401 164 103 BanII G_rGCy'C Cuts at: 0 185 344 551 768 904 967 1027 1293 Size: 185 159 207 217 136 63 60 266 Fragments arranged by size: 266 217 207 185 159 136 63 60 BbeI G_GCGC'C Cuts at: 0 508 1293 Size: 508 785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1055 1089 1293 Size: 1055 34 204 Fragments arranged by size: 1055 204 34 BfrBI ATG'CAT Cuts at: 0 555 1293 Size: 555 738 BglI GCCn_nnn'nGGC Cuts at: 0 851 1293 Size: 851 442 Bme1580I G_kGCm'C Cuts at: 0 768 1134 1293 Size: 768 366 159 Fragments arranged by size: 768 366 159 BmrI ACTGGGnnnn_n' Cuts at: 0 764 968 1293 Size: 764 204 325 Fragments arranged by size: 764 325 204 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 923 1293 Size: 923 370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 66 309 382 502 1142 1293 Size: 66 243 73 120 640 151 Fragments arranged by size: 640 243 151 120 73 66 Bpu10I CC'TnA_GC Cuts at: 0 1145 1293 Size: 1145 148 BsaI GGTCTCn'nnnn_ Cuts at: 0 22 501 1293 Size: 22 479 792 Fragments arranged by size: 792 479 22 BsaAI yAC'GTr Cuts at: 0 392 512 1293 Size: 392 120 781 Fragments arranged by size: 781 392 120 BsaHI Gr'CG_yC Cuts at: 0 505 682 1293 Size: 505 177 611 Fragments arranged by size: 611 505 177 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 931 961 1293 Size: 931 30 332 Fragments arranged by size: 931 332 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 687 1293 Size: 687 606 BseYI C'CCAG_C Cuts at: 0 33 343 561 1236 1293 Size: 33 310 218 675 57 Fragments arranged by size: 675 310 218 57 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1085 1293 Size: 1085 208 BsiEI CG_ry'CG Cuts at: 0 241 594 1293 Size: 241 353 699 Fragments arranged by size: 699 353 241 BsiHKAI G_wGCw'C Cuts at: 0 551 581 1293 Size: 551 30 712 Fragments arranged by size: 712 551 30 Bsp1286I G_dGCh'C Cuts at: 0 185 344 551 581 768 904 967 1027 Size: 185 159 207 30 187 136 63 60 Cuts at: 1027 1134 1293 Size: 107 159 Fragments arranged by size: 207 187 185 159 159 136 107 63 60 30 BspHI T'CATG_A Cuts at: 0 543 1293 Size: 543 750 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 480 1117 1293 Size: 480 637 176 Fragments arranged by size: 637 480 176 BsrBI CCG'CTC Cuts at: 0 907 1293 Size: 907 386 BsrFI r'CCGG_y Cuts at: 0 731 1293 Size: 731 562 BssSI C'ACGA_G Cuts at: 0 574 1293 Size: 574 719 BstXI CCAn_nnnn'nTGG Cuts at: 0 892 1034 1293 Size: 892 142 259 Fragments arranged by size: 892 259 142 BstYI r'GATC_y Cuts at: 0 626 1293 Size: 626 667 Bsu36I CC'TnA_GG Cuts at: 0 131 1293 Size: 131 1162 BtgI C'CryG_G Cuts at: 0 284 864 1293 Size: 284 580 429 Fragments arranged by size: 580 429 284 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 804 1293 Size: 804 489 BtsI GCAGTG_nn' Cuts at: 0 483 1293 Size: 483 810 EaeI y'GGCC_r Cuts at: 0 238 783 1163 1293 Size: 238 545 380 130 Fragments arranged by size: 545 380 238 130 EagI C'GGCC_G Cuts at: 0 238 1293 Size: 238 1055 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 967 1293 Size: 967 326 EcoICRI GAG'CTC Cuts at: 0 549 1293 Size: 549 744 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 66 309 382 502 711 1142 1181 1293 Size: 66 243 73 120 209 431 39 112 Fragments arranged by size: 431 243 209 120 112 73 66 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 220 1077 1124 1293 Size: 220 857 47 169 Fragments arranged by size: 857 220 169 47 EcoO109I rG'GnC_Cy Cuts at: 0 309 765 822 860 875 1012 1293 Size: 309 456 57 38 15 137 281 Fragments arranged by size: 456 309 281 137 57 38 15 FspI TGC'GCA Cuts at: 0 90 1293 Size: 90 1203 HaeII r_GCGC'y Cuts at: 0 73 508 1293 Size: 73 435 785 Fragments arranged by size: 785 435 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 682 714 931 963 1293 Size: 682 32 217 32 330 Fragments arranged by size: 682 330 217 32 32 HincII GTy'rAC Cuts at: 0 615 1293 Size: 615 678 KasI G'GCGC_C Cuts at: 0 504 1293 Size: 504 789 KpnI G_GTAC'C Cuts at: 0 107 1293 Size: 107 1186 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 276 852 1293 Size: 276 576 441 Fragments arranged by size: 576 441 276 MslI CAynn'nnrTG Cuts at: 0 445 556 1032 1293 Size: 445 111 476 261 Fragments arranged by size: 476 445 261 111 MspA1I CmG'CkG Cuts at: 0 86 930 1293 Size: 86 844 363 Fragments arranged by size: 844 363 86 NaeI GCC'GGC Cuts at: 0 733 1293 Size: 733 560 NarI GG'CG_CC Cuts at: 0 505 1293 Size: 505 788 NcoI C'CATG_G Cuts at: 0 284 1293 Size: 284 1009 NgoMIV G'CCGG_C Cuts at: 0 731 1293 Size: 731 562 NsiI A_TGCA'T Cuts at: 0 557 1293 Size: 557 736 NspI r_CATG'y Cuts at: 0 559 678 1293 Size: 559 119 615 Fragments arranged by size: 615 559 119 PfoI T'CCnGG_A Cuts at: 0 188 359 958 1293 Size: 188 171 599 335 Fragments arranged by size: 599 335 188 171 PmlI CAC'GTG Cuts at: 0 392 512 1293 Size: 392 120 781 Fragments arranged by size: 781 392 120 PpuMI rG'GwC_Cy Cuts at: 0 309 822 875 1293 Size: 309 513 53 418 Fragments arranged by size: 513 418 309 53 PshAI GACnn'nnGTC Cuts at: 0 258 1293 Size: 258 1035 PspOMI G'GGCC_C Cuts at: 0 764 1293 Size: 764 529 PstI C_TGCA'G Cuts at: 0 844 929 1011 1293 Size: 844 85 82 282 Fragments arranged by size: 844 282 85 82 PvuII CAG'CTG Cuts at: 0 86 1293 Size: 86 1207 SacI G_AGCT'C Cuts at: 0 551 1293 Size: 551 742 SanDI GG'GwC_CC Cuts at: 0 875 1293 Size: 875 418 SbfI CC_TGCA'GG Cuts at: 0 1011 1293 Size: 1011 282 SfcI C'TryA_G Cuts at: 0 840 925 1007 1293 Size: 840 85 82 286 Fragments arranged by size: 840 286 85 82 SfoI GGC'GCC Cuts at: 0 506 1293 Size: 506 787 SmaI CCC'GGG Cuts at: 0 820 1293 Size: 820 473 SmlI C'TyrA_G Cuts at: 0 462 1293 Size: 462 831 StuI AGG'CCT Cuts at: 0 145 1072 1119 1293 Size: 145 927 47 174 Fragments arranged by size: 927 174 145 47 StyI C'CwwG_G Cuts at: 0 25 63 177 284 1293 Size: 25 38 114 107 1009 Fragments arranged by size: 1009 114 107 38 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 18 277 443 1022 1293 Size: 18 259 166 579 271 Fragments arranged by size: 579 271 259 166 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 786 818 1293 Size: 786 32 475 Fragments arranged by size: 786 475 32 XhoI C'TCGA_G Cuts at: 0 462 1293 Size: 462 831 XmaI C'CCGG_G Cuts at: 0 818 1293 Size: 818 475 Enzymes that do cut: AarI Acc65I AclI AcuI AflIII AleI AlfI AlwNI ApaI AvaI AvrII BanI BanII BbeI BcgI BfrBI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SbfI SfcI SfoI SmaI SmlI StuI StyI TaqII TstI XhoI XmaI Enzymes that do not cut: AatII AccI AfeI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbsI BbvCI BciVI BclI BglII BlpI BmgBI BmtI BpuEI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EarI EcoRI EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI MscI NdeI NheI NotI NruI PacI PasI PciI PflMI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XmnI ZraI