################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccI AccIII AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI BalI BamHI BbsI BbvCI BciVI BclI BfrI BglII BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV FbaI FseI HindIII HpaI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PshBI PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 103 GGTACC G^GTACC AccII 584 CGCG CG^CG AclI 383 AACGTT AA^CGTT AflIII 511 ACGTGT A^C[GA][CT]GT AocI 130 CCTGAGG CC^T[ACGT]AGG ApaI 764 GGGCCC GGGCC^C Asp718I 103 GGTACC G^GTACC AviII 88 TGCGCA TGC^GCA AvrII 63 CCTAGG C^CTAGG BbeI 504 GGCGCC GGCGC^C BcgI 1066 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 845 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 63 CCTAGG C^CTAGG BseRI 696 CTCCTC GAGGAG(10/8) BsgI 1064 GTGCAG GTGCAG(16/14) BspHI 543 TCATGA T^CATGA BsrBI 905 CCGCTC CCGCTC(-3/-3) BsrFI 731 GCCGGC [GA]^CCGG[CT] BssSI 574 CACGAG CACGAG(-5/-1) BstUI 584 CGCG CG^CG BstYI 626 AGATCC [GA]^GATC[CT] Bsu36I 130 CCTGAGG CC^T[ACGT]AGG Cfr10I 731 GCCGGC [GA]^CCGG[CT] EagI 238 CGGCCG C^GGCCG EclXI 238 CGGCCG C^GGCCG Eco52I 238 CGGCCG C^GGCCG Eco81I 130 CCTGAGG CC^T[ACGT]AGG EcoT22I 553 ATGCAT ATGCA^T FspI 88 TGCGCA TGC^GCA HgaI 681 GACGC GACGC(5/10) HincII 613 GTCAAC GT[CT]^[GA]AC HindII 613 GTCAAC GT[CT]^[GA]AC KasI 504 GGCGCC G^GCGCC KpnI 103 GGTACC GGTAC^C MaeIII 406 GTCAC ^GT[ACGT]AC MflI 626 AGATCC [GA]^GATC[CT] MseI 173 TTAA T^TAA MvnI 584 CGCG CG^CG NaeI 731 GCCGGC GCC^GGC NarI 504 GGCGCC GG^CGCC NcoI 284 CCATGG C^CATGG NgoMIV 731 GCCGGC G^CCGGC NsiI 553 ATGCAT ATGCA^T PaeR7I 462 CTCGAG C^TCGAG PshAI 254 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 383 AACGTT AA^CGTT PvuII 84 CAGCTG CAG^CTG RcaI 543 TCATGA T^CATGA SacI 547 GAGCTC GAGCT^C SbfI 1006 CCTGCAGG CCTGCA^GG SfoI 504 GGCGCC GGC^GCC SmaI 818 CCCGGG CCC^GGG SmlI 462 CTCGAG C^T[CT][GA]AG Sse8387I 1006 CCTGCAGG CCTGCA^GG Tru9I 173 TTAA T^TAA Tsp45I 406 GTCAC ^GT[GC]AC Tsp509I 171 AATT ^AATT XhoI 462 CTCGAG C^TCGAG XhoII 626 AGATCC [GA]^GATC[CT] XmaCI 818 CCCGGG C^CCGGG XmaI 818 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 504 GGCGCC G[GA]^CG[CT]C 681 GACGCC AlwI 60 GATCC GGATC(4/5) 627 GATCC AspHI 547 GAGCTC G[AT]GC[AT]^C 577 GAGCAC AvaI 462 CTCGAG C^[CT]CG[GA]G 818 CCCGGG BbrPI 390 CACGTG CAC^GTG 510 CACGTG BfaI 64 CTAG C^TAG 1262 CTAG BsaAI 390 CACGTG [CT]AC^GT[GA] 510 CACGTG BsaHI 504 GGCGCC G[GA]^CG[CT]C 681 GACGCC BsaI 16 GGTCTC GGTCTC(1/5) 495 GGTCTC BsiEI 238 CGGCCG CG[GA][CT]^CG 591 CGACCG BsiHKAI 547 GAGCTC G[AT]GC[AT]^C 577 GAGCAC BsoBI 462 CTCGAG C^[CT]CG[GA]G 818 CCCGGG BspMI 489 GCAGGT ACCTGC(4/8) 1126 GCAGGT BstXI 885 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1027 CCACATGGGTGG DsaI 284 CCATGG C^C[GA][CT]GG 864 CCGTGG HaeII 69 AGCGCC [GA]GCGC^[CT] 504 GGCGCC Hin1I 504 GGCGCC G[GA]^CG[CT]C 681 GACGCC MaeI 64 CTAG C^TAG 1262 CTAG MspA1I 84 CAGCTG C[AC]G^C[GT]G 928 CAGCGG NspI 555 GCATGT [GA]CATG^[CT] 674 ACATGC PleI 243 GAGTC GAGTC(4/5) 1039 GAGTC PmaCI 390 CACGTG CAC^GTG 510 CACGTG PmlI 390 CACGTG CAC^GTG 510 CACGTG TaqI 221 TCGA T^CGA 463 TCGA TfiI 955 GAATC G^A[AT]TC 1208 GAATC TthHB8I 221 TCGA T^CGA 463 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TseI TspRI