TRIM74 ORF clone pFN21AE5223

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5223

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Clone info.

Species

human

Clone Name

pFN21AE5223 Sequence (with vector) Restriction Map

Product ID

FHC09506

ORF Sequence

DNA sequence (747 bp)
Protein sequence (249 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5223

Symbol

TRIM74

Alias : TRIM50C

Description

tripartite motif containing 74

Original Clone ID

oc95223

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 747 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 249 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34665.1 2.5e-77 98.8 TRIM73 CCDS5545.1 7.4e-77 98.4 TRIM74 CCDS34654.1 5.3e-70 92.8 TRIM50 CCDS32437.1 1.9e-12 28.2 TRIM72 CCDS4654.1 1.5e-11 32.3 TRIM27 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 16 56 PF00097 Zinc finger IPR000315 85 125 PF00643 Zinc finger HMMSmart IPR001841 16 56 SM00184 Zinc finger IPR000315 84 125 SM00336 Zinc finger ProfileScan IPR001841 16 56 PS50089 Zinc finger IPR000315 84 125 PS50119 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011514491 249 612550 98.8 100.0 NP_944606 250 612549 98.8 100.0 NP_001304744 250 612550 98.4 100.0 NP_942150 250 612550 98.4 100.0 XP_011514489 250 612550 98.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5223 Download

>KIEE5223  747 bp
ATGGCTTGGCAGGTGAGCCTGCTGGAGCTGGAGGACCGGCTTCAGTGTCCCATCTGCCTG
GAGGTCTTCAAGGAGTCCCTAATGCTACAGTGCGGCCACTCCTACTGCAAGGGCTGCCTG
GTTTCCCTGTCCTACCACCTGGACACCAAGGTGCGCTGCCCCATGTGCTGGCAGGTGGTG
GACGGCAGCAGCTCCTTGCCCAACGTCTCCCTGGCCTGGGTGATCGAAGCCCTGAGGCTC
CCTGGGGACCCGGAGCCCAAGGTCTGCGTGCACCACCGGAACCCGCTCAGCCTTTTCTGC
GAGAAGGACCAGGAGCTCATCTGTGGCCTCTGCGGTCTGCTGGGCTCCCACCAACACCAC
CCGGTCACGCCCGTCTCCACCATCTGCAGCCGCATGAAGGAGGAGCTCGCAGCCCTCTTC
TCTGAGCTGAAGCAGGAGCAGAAGAAGGTGGATGAGCTCATCGCCAAACTGGTGAACAAC
CGGACCCGAATCGTCAATGAGTCGGATGTCTTCAGCTGGGTGATCCGCCGCGAGTTCCAG
GAGCTGCGCCACCCGGTGGACGAGGAGAAGGCCCGCTGCCTGGAGGGGATAGGGGGTCAC
ACCCGTGGCCTGGTGGCCTCCCTGGACATGCAGCTGGAGCAGGCCCAGGGAACCCGGGAG
CGGCTGGCCCAAGCCGAGTGTGTGCTGGAACAGTTCGGAAATGAGGACCACCATGAGTTC
ATCTGGTTCCACTCCATGGCCTCCAGG

ORF Protein

Cloned ORF protein sequence for pFN21AE5223 Download

>KIEE5223  249 aa
MAWQVSLLELEDRLQCPICLEVFKESLMLQCGHSYCKGCLVSLSYHLDTKVRCPMCWQVV
DGSSSLPNVSLAWVIEALRLPGDPEPKVCVHHRNPLSLFCEKDQELICGLCGLLGSHQHH
PVTPVSTICSRMKEELAALFSELKQEQKKVDELIAKLVNNRTRIVNESDVFSWVIRREFQ
ELRHPVDEEKARCLEGIGGHTRGLVASLDMQLEQAQGTRERLAQAECVLEQFGNEDHHEF
IWFHSMASR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5223 Download

>pFN21AE5223  5452 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTTGGCAGGTGAGCCTGCTGGAGCTGGAGGA
CCGGCTTCAGTGTCCCATCTGCCTGGAGGTCTTCAAGGAGTCCCTAATGCTACAGTGCGG
CCACTCCTACTGCAAGGGCTGCCTGGTTTCCCTGTCCTACCACCTGGACACCAAGGTGCG
CTGCCCCATGTGCTGGCAGGTGGTGGACGGCAGCAGCTCCTTGCCCAACGTCTCCCTGGC
CTGGGTGATCGAAGCCCTGAGGCTCCCTGGGGACCCGGAGCCCAAGGTCTGCGTGCACCA
CCGGAACCCGCTCAGCCTTTTCTGCGAGAAGGACCAGGAGCTCATCTGTGGCCTCTGCGG
TCTGCTGGGCTCCCACCAACACCACCCGGTCACGCCCGTCTCCACCATCTGCAGCCGCAT
GAAGGAGGAGCTCGCAGCCCTCTTCTCTGAGCTGAAGCAGGAGCAGAAGAAGGTGGATGA
GCTCATCGCCAAACTGGTGAACAACCGGACCCGAATCGTCAATGAGTCGGATGTCTTCAG
CTGGGTGATCCGCCGCGAGTTCCAGGAGCTGCGCCACCCGGTGGACGAGGAGAAGGCCCG
CTGCCTGGAGGGGATAGGGGGTCACACCCGTGGCCTGGTGGCCTCCCTGGACATGCAGCT
GGAGCAGGCCCAGGGAACCCGGGAGCGGCTGGCCCAAGCCGAGTGTGTGCTGGAACAGTT
CGGAAATGAGGACCACCATGAGTTCATCTGGTTCCACTCCATGGCCTCCAGGGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT