(Linear) (Six-base) MAPSORT of: KIEE5223.seq Check: 7187 from: 1 to: 747 KIEE5223.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 162 747 Size: 162 585 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 448 495 747 Size: 25 423 47 252 Fragments arranged by size: 423 252 47 25 AleI CACnn'nnGTG Cuts at: 0 148 602 747 Size: 148 454 145 Fragments arranged by size: 454 148 145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 211 747 Size: 211 536 AlwNI CAG_nnn'CTG Cuts at: 0 543 747 Size: 543 204 ApaLI G'TGCA_C Cuts at: 0 268 747 Size: 268 479 AvaI C'yCGr_G Cuts at: 0 653 747 Size: 653 94 BanII G_rGCy'C Cuts at: 0 257 317 346 407 458 747 Size: 257 60 29 61 51 289 Fragments arranged by size: 289 257 61 60 51 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 57 501 747 Size: 57 444 246 Fragments arranged by size: 444 246 57 BlpI GC'TnA_GC Cuts at: 0 286 747 Size: 286 461 Bme1580I G_kGCm'C Cuts at: 0 272 747 Size: 272 475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 49 79 601 655 726 747 Size: 43 6 30 522 54 71 21 Fragments arranged by size: 522 71 54 43 30 21 6 BsaWI w'CCGG_w Cuts at: 0 275 479 747 Size: 275 204 268 Fragments arranged by size: 275 268 204 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 577 747 Size: 415 162 170 Fragments arranged by size: 415 170 162 BseYI C'CCAG_C Cuts at: 0 339 515 747 Size: 339 176 232 Fragments arranged by size: 339 232 176 BsiHKAI G_wGCw'C Cuts at: 0 272 317 407 458 747 Size: 272 45 90 51 289 Fragments arranged by size: 289 272 90 51 45 BsmBI CGTCTCn'nnnn_ Cuts at: 0 210 378 747 Size: 210 168 369 Fragments arranged by size: 369 210 168 Bsp1286I G_dGCh'C Cuts at: 0 257 272 317 346 407 458 747 Size: 257 15 45 29 61 51 289 Fragments arranged by size: 289 257 61 51 45 29 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 162 747 Size: 162 585 BsrBI CCG'CTC Cuts at: 0 285 660 747 Size: 285 375 87 Fragments arranged by size: 375 285 87 BsrFI r'CCGG_y Cuts at: 0 35 747 Size: 35 712 BstAPI GCAn_nnn'nTGC Cuts at: 0 113 747 Size: 113 634 BstXI CCAn_nnnn'nTGG Cuts at: 0 57 747 Size: 57 690 Bsu36I CC'TnA_GG Cuts at: 0 232 747 Size: 232 515 BtgI C'CryG_G Cuts at: 0 603 734 747 Size: 603 131 13 Fragments arranged by size: 603 131 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 444 747 Size: 444 303 DraIII CAC_nnn'GTG Cuts at: 0 555 747 Size: 555 192 EaeI y'GGCC_r Cuts at: 0 93 747 Size: 93 654 EarI CTCTTCn'nnn_ Cuts at: 0 421 747 Size: 421 326 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 514 747 Size: 514 233 EcoICRI GAG'CTC Cuts at: 0 315 405 456 747 Size: 315 90 51 291 Fragments arranged by size: 315 291 90 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 43 49 79 448 495 601 655 Size: 25 18 6 30 369 47 106 54 Cuts at: 655 726 747 Size: 71 21 Fragments arranged by size: 369 106 71 54 47 30 25 21 18 6 EcoO109I rG'GnC_Cy Cuts at: 0 246 747 Size: 246 501 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 579 611 747 Size: 579 32 136 Fragments arranged by size: 579 136 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 482 747 Size: 482 265 MslI CAynn'nnrTG Cuts at: 0 148 602 747 Size: 148 454 145 Fragments arranged by size: 454 148 145 MspA1I CmG'CkG Cuts at: 0 515 575 633 747 Size: 515 60 58 114 Fragments arranged by size: 515 114 60 58 NcoI C'CATG_G Cuts at: 0 734 747 Size: 734 13 NspI r_CATG'y Cuts at: 0 630 747 Size: 630 117 PasI CC'CwG_GG Cuts at: 0 241 645 747 Size: 241 404 102 Fragments arranged by size: 404 241 102 PflMI CCAn_nnn'nTGG Cuts at: 0 167 555 735 747 Size: 167 388 180 12 Fragments arranged by size: 388 180 167 12 PfoI T'CCnGG_A Cuts at: 0 536 747 Size: 536 211 PpuMI rG'GwC_Cy Cuts at: 0 246 747 Size: 246 501 PstI C_TGCA'G Cuts at: 0 388 747 Size: 388 359 PvuII CAG'CTG Cuts at: 0 515 633 747 Size: 515 118 114 Fragments arranged by size: 515 118 114 SacI G_AGCT'C Cuts at: 0 317 407 458 747 Size: 317 90 51 289 Fragments arranged by size: 317 289 90 51 SanDI GG'GwC_CC Cuts at: 0 246 747 Size: 246 501 SfcI C'TryA_G Cuts at: 0 85 384 747 Size: 85 299 363 Fragments arranged by size: 363 299 85 SmaI CCC'GGG Cuts at: 0 655 747 Size: 655 92 StyI C'CwwG_G Cuts at: 0 146 257 734 747 Size: 146 111 477 13 Fragments arranged by size: 477 146 111 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 207 507 747 Size: 207 300 240 Fragments arranged by size: 300 240 207 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 207 747 Size: 207 540 XmaI C'CCGG_G Cuts at: 0 653 747 Size: 653 94 Enzymes that do cut: AarI AcuI AleI AlfI AlwNI ApaLI AvaI BanII BbsI BlpI Bme1580I BpmI BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI Bsu36I BtgI BtgZI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I Hin4I MmeI MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PstI PvuII SacI SanDI SfcI SmaI StyI TaqII XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtsI ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI