Restriction map for
pFN21AE5223
(Circular) (Six-base) MAPSORT of: pFN21AE5223.seq Check: 7533 from: 1 to: 5452
pFN21AE5223 5452 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2005 2167 2005
Size: 162 5290
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 1937
Size: 858 4594
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2453 2500 3515 4899 5167 1632
Size: 398 423 47 1015 1384 268 1917
Fragments arranged by size:
1917 1384 1015 423 398 268 47
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5255
AflIII A'CryG_T
Cuts at: 1203 4351 5328 1203
Size: 3148 977 1327
Fragments arranged by size:
3148 1327 977
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 2153 2607 1166
Size: 987 454 4011
Fragments arranged by size:
4011 987 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 1184
Size: 1032 4420
AlwNI CAG_nnn'CTG
Cuts at: 2548 3195 4767 2548
Size: 647 1572 3233
Fragments arranged by size:
3233 1572 647
ApaLI G'TGCA_C
Cuts at: 2273 3509 4665 2273
Size: 1236 1156 3060
Fragments arranged by size:
3060 1236 1156
ApoI r'AATT_y
Cuts at: 1493 2761 2987 3139 5248 1493
Size: 1268 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2658 2777 1870
Size: 73 715 119 4545
Fragments arranged by size:
4545 715 119 73
BamHI G'GATC_C
Cuts at: 2782 3149 2782
Size: 367 5085
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2773 4228 618
Size: 325 217 627 986 1455 1842
Fragments arranged by size:
1842 1455 986 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2262 2322 2351 2412 2463 2771 1391
Size: 871 60 29 61 51 308 4072
Fragments arranged by size:
4072 871 308 61 60 51 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2062 2506 928
Size: 971 163 444 3874
Fragments arranged by size:
3874 971 444 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3643 3677 1903
Size: 34 1706 34 3678
Fragments arranged by size:
3678 1706 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4063 136
Size: 107 122 71 631 605 2391 1525
Fragments arranged by size:
2391 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2291 2869 2291
Size: 578 4874
Bme1580I G_kGCm'C
Cuts at: 1790 2277 3513 4669 1790
Size: 487 1236 1156 2573
Fragments arranged by size:
2573 1236 1156 487
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3183 4141 442
Size: 416 53 83 399 24 1766 958 1753
Fragments arranged by size:
1766 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2054 2084 2606 2660 2731 4112 1239
Size: 809 6 30 522 54 71 1381 2579
Fragments arranged by size:
2579 1381 809 522 71 54 30 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3577 4442 4740 3577
Size: 865 298 4289
Fragments arranged by size:
4289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4115 882
Size: 617 2616 2219
Fragments arranged by size:
2616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3243 493
Size: 711 2039 2702
Fragments arranged by size:
2702 2039 711
BsaBI GATnn'nnATC
Cuts at: 5446 5446
Size: 5452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3259 3641 4276 275
Size: 53 83 186 936 1726 382 635 1451
Fragments arranged by size:
1726 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2280 2484 3885 4283 4557 4704 5115 2280
Size: 204 1401 398 274 147 411 2617
Fragments arranged by size:
2617 1401 411 398 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5227
Fragments arranged by size:
5227 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2582 1186
Size: 1234 162 4056
Fragments arranged by size:
4056 1234 162
BseYI C'CCAG_C
Cuts at: 2344 2520 4655 5151 2344
Size: 176 2135 496 2645
Fragments arranged by size:
2645 2135 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4879
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 3663 4691 1540
Size: 275 186 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2322 2412 2463 3513 3598 4669 2277
Size: 45 90 51 1050 85 1071 3060
Fragments arranged by size:
3060 1071 1050 90 85 51 45
BsmI GAATG_Cn'
Cuts at: 2963 3056 2963
Size: 93 5359
BsmBI CGTCTCn'nnnn_
Cuts at: 2215 2383 2215
Size: 168 5284
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2277 2322 2351 2412 2463 2771
Size: 399 472 15 45 29 61 51 308
Cuts at: 2771 3513 3598 4669 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 308 85 61
51 45 29 15
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 3341 5071 3341
Size: 1730 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 2167 2807 844
Size: 1161 162 640 3489
Fragments arranged by size:
3489 1161 640 162
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 2665 3339 1394
Size: 429 467 375 674 3507
Fragments arranged by size:
3507 674 467 429 375
BsrDI GCAATG_nn'
Cuts at: 65 1248 3947 4121 65
Size: 1183 2699 174 1396
Fragments arranged by size:
2699 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 4096 4283 1700
Size: 110 230 2056 187 2869
Fragments arranged by size:
2869 2056 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5452
BssSI C'ACGA_G
Cuts at: 3512 4524 3512
Size: 1012 4440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2118 794
Size: 1324 4128
BstBI TT'CG_AA
Cuts at: 4256 4256
Size: 5452
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5452
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5452
BstYI r'GATC_y
Cuts at: 1972 2782 3149 3534 3551 5003 5089 5101 5447
Size: 810 367 385 17 1452 86 12 346
Cuts at: 5447 1972
Size: 1977
Fragments arranged by size:
1977 1452 810 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2237 2237
Size: 5452
BtgI C'CryG_G
Cuts at: 513 1065 2004 2608 2739 513
Size: 552 939 604 131 3226
Fragments arranged by size:
3226 939 604 552 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2449 487
Size: 683 56 1223 3490
Fragments arranged by size:
3490 1223 683 56
BtsI GCAGTG_nn'
Cuts at: 2968 3762 3782 2968
Size: 794 20 4638
Fragments arranged by size:
4638 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5417
DraI TTT'AAA
Cuts at: 2756 2756
Size: 5452
DraIII CAC_nnn'GTG
Cuts at: 2560 2560
Size: 5452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4459 809
Size: 507 3143 1802
Fragments arranged by size:
3143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2899 3788
Size: 54 1425 50 275 18 268 801 889
Cuts at: 3788 5417 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 801 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2899 1537
Size: 275 1087 4090
Fragments arranged by size:
4090 1087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 3382 1353
Size: 1073 956 3423
Fragments arranged by size:
3423 1073 956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 4039 4413 4559 387
Size: 1064 138 930 1520 374 146 1280
Fragments arranged by size:
1520 1280 1064 930 374 146 138
EcoICRI GAG'CTC
Cuts at: 2320 2410 2461 2320
Size: 90 51 5311
Fragments arranged by size:
5311 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2048 2054 2084 2453 2500 2606
Size: 393 398 18 6 30 369 47 106
Cuts at: 2606 2660 2731 3515 4112 4899 5167 1239
Size: 54 71 784 597 787 268 1524
Fragments arranged by size:
1524 787 784 597 398 393 369 268
106 71 54 47 30 18 6
EcoO109I rG'GnC_Cy
Cuts at: 2251 2251
Size: 5452
EcoRI G'AATT_C
Cuts at: 2761 3139 2761
Size: 378 5074
FspI TGC'GCA
Cuts at: 3958 3958
Size: 5452
HaeII r_GCGC'y
Cuts at: 1678 4599 1678
Size: 2921 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2584 2616 4093 4125 4167
Size: 32 135 32 1012 32 1477 32 42
Cuts at: 4167 4199 1373
Size: 32 2626
Fragments arranged by size:
2626 1477 1012 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3042 669
Size: 988 281 858 246 3079
Fragments arranged by size:
3079 988 858 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5452
HpaI GTT'AAC
Cuts at: 3042 3042
Size: 5452
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 5452
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 4566 4750 1305
Size: 1182 2079 184 2007
Fragments arranged by size:
2079 2007 1182 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3630
Fragments arranged by size:
3630 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2607 3410 3769 3928 518
Size: 648 987 454 803 359 159 2042
Fragments arranged by size:
2042 987 803 648 454 359 159
MspA1I CmG'CkG
Cuts at: 1606 2520 2580 2638 2868 3545 4693 4938 1606
Size: 914 60 58 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 677 245 230 60 58
NcoI C'CATG_G
Cuts at: 513 1065 2004 2739 513
Size: 552 939 735 3226
Fragments arranged by size:
3226 939 735 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2635 2810 4355 1132
Size: 97 1406 175 1545 2229
Fragments arranged by size:
2229 1545 1406 175 97
PasI CC'CwG_GG
Cuts at: 1348 2246 2650 1348
Size: 898 404 4150
Fragments arranged by size:
4150 898 404
PciI A'CATG_T
Cuts at: 4351 4351
Size: 5452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2172 2560 2740 1108
Size: 902 162 388 180 3820
Fragments arranged by size:
3820 902 388 180 162
PfoI T'CCnGG_A
Cuts at: 2541 2541
Size: 5452
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2251
Size: 5452
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5452
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PstI C_TGCA'G
Cuts at: 830 2393 2804 830
Size: 1563 411 3478
Fragments arranged by size:
3478 1563 411
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
PvuII CAG'CTG
Cuts at: 2520 2638 2520
Size: 118 5334
SacI G_AGCT'C
Cuts at: 2322 2412 2463 2322
Size: 90 51 5311
Fragments arranged by size:
5311 90 51
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SanDI GG'GwC_CC
Cuts at: 2251 2251
Size: 5452
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SfcI C'TryA_G
Cuts at: 826 1045 2090 2389 2800 3935 4616 4807 826
Size: 219 1045 299 411 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 681 411 299 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5452
SmaI CCC'GGG
Cuts at: 1872 2660 2779 1872
Size: 788 119 4545
Fragments arranged by size:
4545 788 119
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3556 4457 4719 820
Size: 197 926 1613 901 262 1553
Fragments arranged by size:
1613 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5452
SphI G_CATG'C
Cuts at: 1132 2810 1132
Size: 1678 3774
SspI AAT'ATT
Cuts at: 5 52 3376 5
Size: 47 3324 2081
Fragments arranged by size:
3324 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 2262 2739 2891 513
Size: 552 939 147 111 477 152 3074
Fragments arranged by size:
3074 939 552 477 152 147 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2212 2512 3479 3496 3649 3834 4279
Size: 1255 632 300 967 17 153 185 445
Cuts at: 4279 325
Size: 1498
Fragments arranged by size:
1498 1255 967 632 445 300 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3698 5410 96
Size: 274 80 33 51 1443 1721 1712 138
Fragments arranged by size:
1721 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5412 5444 5412
Size: 32 5420
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5452
XmaI C'CCGG_G
Cuts at: 1870 2658 2777 1870
Size: 788 119 4545
Fragments arranged by size:
4545 788 119
XmnI GAAnn'nnTTC
Cuts at: 3581 3581
Size: 5452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI