Restriction map for
pFN21AE5223
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 529 CGCG CG^CG
Alw44I 268 GTGCAC G^TGCAC
AlwI 522 GATCC GGATC(4/5)
AlwNI 538 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 231 CCTGAGG CC^T[ACGT]AGG
ApaLI 268 GTGCAC G^TGCAC
AvaI 653 CCCGGG C^[CT]CG[GA]G
BlpI 285 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 285 GCTCAGC GC^T[ACGT]AGC
BsoBI 653 CCCGGG C^[CT]CG[GA]G
BsrFI 35 ACCGGC [GA]^CCGG[CT]
BsrI 468 ACTGG ACTGG(1/-1)
BstAPI 107 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 529 CGCG CG^CG
BstXI 50 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 231 CCTGAGG CC^T[ACGT]AGG
CelII 285 GCTCAGC GC^T[ACGT]AGC
Cfr10I 35 ACCGGC [GA]^CCGG[CT]
DraII 245 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 550 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 93 CGGCCA [CT]^GGCC[GA]
EarI 415 CTCTTC CTCTTC(1/4)
Eco81I 231 CCTGAGG CC^T[ACGT]AGG
EcoO109I 245 GGGACCC [GA]G^G[ACGT]CC[CT]
Ksp632I 415 CTCTTC CTCTTC(1/4)
MaeII 203 ACGT A^CGT
MvnI 529 CGCG CG^CG
NcoI 734 CCATGG C^CATGG
NspI 626 ACATGC [GA]CATG^[CT]
PpuMI 245 GGGACCC [GA]G^G[AT]CC[CT]
PstI 384 CTGCAG CTGCA^G
SmaI 653 CCCGGG CCC^GGG
TaqI 224 TCGA T^CGA
TfiI 488 GAATC G^A[AT]TC
TthHB8I 224 TCGA T^CGA
XcmI 200 CCAACGTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 653 CCCGGG C^CCGGG
XmaI 653 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BbsI 64 GTCTTC GAAGAC(2/6)
508 GTCTTC
BpuAI 64 GTCTTC GAAGAC(2/6)
508 GTCTTC
BsaWI 275 ACCGGA [AT]^CCGG[AT]
479 ACCGGA
BseRI 400 GAGGAG GAGGAG(10/8)
562 GAGGAG
BsmAI 205 GTCTC GTCTC(1/5)
373 GTCTC
BsmBI 204 CGTCTC CGTCTC(1/5)
372 CGTCTC
BspMI 9 GCAGGT ACCTGC(4/8)
171 GCAGGT
BsrBI 283 CCGCTC CCGCTC(-3/-3)
658 GAGCGG
BstF5I 450 GGATG GGATG(2/0)
504 GGATG
CfoI 153 GCGC GCG^C
546 GCGC
DpnII 222 GATC ^GATC
522 GATC
DsaI 603 CCGTGG C^C[GA][CT]GG
734 CCATGG
FauI 282 CCCGC CCCGC(4/6)
572 CCCGC
FokI 450 GGATG GGATG(9/13)
504 GGATG
HhaI 153 GCGC GCG^C
546 GCGC
HinP1I 153 GCGC G^CGC
546 GCGC
MaeIII 364 GTCAC ^GT[ACGT]AC
596 GTCAC
MboI 222 GATC ^GATC
522 GATC
MslI 144 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
598 CACACCCGTG
NdeII 222 GATC ^GATC
522 GATC
PleI 73 GAGTC GAGTC(4/5)
499 GAGTC
PvuII 513 CAGCTG CAG^CTG
631 CAGCTG
Sau3AI 222 GATC ^GATC
522 GATC
SfcI 85 CTACAG C^T[GA][CT]AG
384 CTGCAG
Tsp45I 364 GTCAC ^GT[GC]AC
596 GTCAC
TspRI 43 CAGTG CA[GC]TG[ACGT][ACGT]^
88 CAGTG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PflMI SacI
Sau96I ScrFI StyI TseI Van91I