KCNK12 ORF clone pFN21AE5021

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5021

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Clone info.

Species

human

Clone Name

pFN21AE5021 Sequence (with vector) Restriction Map

Product ID

FHC20998

ORF Sequence

DNA sequence (1290 bp)
Protein sequence (430 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5021

Symbol

KCNK12

Alias : K2p12.1, THIK-2, THIK2

Description

potassium channel, two pore domain subfamily K, member 12

Original Clone ID

cp95021

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1290 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 430 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1835.1 1.7e-178 100.0 KCNK12 CCDS9889.1 2.4e-104 65.5 KCNK13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR005410 32 47 PR01588 Potassium channel IPR005410 87 99 PR01588 Potassium channel IPR005410 101 113 PR01588 Potassium channel IPR003280 125 153 PR01333 Potassium channel IPR005410 153 167 PR01588 Potassium channel IPR005410 232 245 PR01588 Potassium channel IPR003280 255 264 PR01333 Potassium channel IPR005410 280 292 PR01588 Potassium channel IPR005410 381 393 PR01588 Potassium channel HMMPfam IPR013099 112 168 PF07885 Ion transport 2 IPR013099 224 302 PF07885 Ion transport 2
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 38 RFVLLAALIGLYLVAGATVFSAL 60 PRIMARY 23 2 116 FPGAFYFVGTVVSTIGFGMTTP 137 SECONDARY 22 3 149 YGLFGCAGTILFFNLFLERIISL 171 PRIMARY 23 4 214 YHVLLILGLFAVLLSCCASAMYT 236 PRIMARY 23 5 280 NFLFILLGVCCIYSLFNVISILI 302 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_071338 430 607366 100.0 100.0 NP_071337 408 607367 65.5 93.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5021 Download

>KIEE5021  1290 bp
ATGTCCTCCCGCAGCCCCCGGCCCCCGCCCCGCCGTAGCCGCCGCCGCCTGCCGCGCCCC
TCCTGCTGCTGCTGCTGCTGCCGCCGTTCGCACCTCAACGAGGACACCGGCCGCTTCGTG
CTGCTGGCGGCGCTCATCGGCCTCTACCTGGTGGCGGGTGCCACAGTCTTCTCGGCGCTC
GAGAGCCCCGGCGAGGCGGAGGCGCGGGCGCGCTGGGGCGCCACGCTGCGCAACTTCAGC
GCTGCGCACGGCGTGGCCGAGCCAGAGCTGCGCGCCTTCCTCCGGCACTACGAGGCCGCG
CTGGCCGCCGGCGTCCGCGCCGACGCGCTGCGCCCGCGCTGGGACTTCCCCGGCGCCTTC
TACTTCGTGGGCACCGTGGTGTCAACCATAGGTTTCGGCATGACCACCCCCGCGACGGTG
GGCGGGAAGGCCTTCCTCATCGCCTACGGGCTGTTCGGCTGCGCTGGGACCATCCTGTTC
TTCAACCTCTTCCTGGAGCGCATCATCTCGCTGCTGGCCTTCATCATGCGCGCCTGCCGG
GAGCGCCAGCTGCGCCGCAGCGGCCTGCTGCCCGCCACCTTCCGCCGCGGCTCCGCGCTC
TCGGAGGCCGACAGCCTGGCGGGCTGGAAGCCCTCGGTGTACCACGTGCTGCTCATCCTG
GGCCTGTTCGCCGTGCTGCTGTCCTGCTGCGCCTCGGCCATGTACACCAGCGTGGAGGGT
TGGGACTACGTGGACTCGCTCTACTTCTGCTTCGTCACCTTCAGCACCATCGGCTTCGGG
GACCTGGTGAGCAGCCAGCACGCCGCCTACCGGAACCAGGGGCTCTACCGCCTGGGCAAC
TTCCTCTTCATCCTGCTCGGCGTGTGCTGCATTTACTCGCTCTTCAACGTCATCTCCATC
CTCATCAAGCAGGTGCTCAACTGGATGCTGCGCAAGCTGAGCTGCCGCTGCTGCGCGCGC
TGCTGCCCGGCTCCTGGCGCGCCCCTGGCCCGGCGCAATGCCATCACCCCAGGCTCCCGG
CTGCGCCGCCGCCTGGCCGCGCTCGGTGCCGACCCCGCGGCCCGCGACAGCGACGCCGAG
GGCCGCCGCCTCTCGGGCGAGCTCATCTCCATGCGCGACCTCACGGCCTCCAACAAGGTG
TCGCTGGCGCTGCTGCAGAAGCAGCTGTCGGAGACGGCCAACGGCTACCCGCGCAGCGTG
TGCGTCAACACGCGCCAGAACGGCTTCTCGGGCGGCGTGGGCGCGCTGGGCATCATGAAC
AACCGGCTGGCCGAGACCAGCGCCTCCAGG

ORF Protein

Cloned ORF protein sequence for pFN21AE5021 Download

>KIEE5021  430 aa
MSSRSPRPPPRRSRRRLPRPSCCCCCCRRSHLNEDTGRFVLLAALIGLYLVAGATVFSAL
ESPGEAEARARWGATLRNFSAAHGVAEPELRAFLRHYEAALAAGVRADALRPRWDFPGAF
YFVGTVVSTIGFGMTTPATVGGKAFLIAYGLFGCAGTILFFNLFLERIISLLAFIMRACR
ERQLRRSGLLPATFRRGSALSEADSLAGWKPSVYHVLLILGLFAVLLSCCASAMYTSVEG
WDYVDSLYFCFVTFSTIGFGDLVSSQHAAYRNQGLYRLGNFLFILLGVCCIYSLFNVISI
LIKQVLNWMLRKLSCRCCARCCPAPGAPLARRNAITPGSRLRRRLAALGADPAARDSDAE
GRRLSGELISMRDLTASNKVSLALLQKQLSETANGYPRSVCVNTRQNGFSGGVGALGIMN
NRLAETSASR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5021 Download

>pFN21AE5021  5995 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCTCCCGCAGCCCCCGGCCCCCGCCCCGCCG
TAGCCGCCGCCGCCTGCCGCGCCCCTCCTGCTGCTGCTGCTGCTGCCGCCGTTCGCACCT
CAACGAGGACACCGGCCGCTTCGTGCTGCTGGCGGCGCTCATCGGCCTCTACCTGGTGGC
GGGTGCCACAGTCTTCTCGGCGCTCGAGAGCCCCGGCGAGGCGGAGGCGCGGGCGCGCTG
GGGCGCCACGCTGCGCAACTTCAGCGCTGCGCACGGCGTGGCCGAGCCAGAGCTGCGCGC
CTTCCTCCGGCACTACGAGGCCGCGCTGGCCGCCGGCGTCCGCGCCGACGCGCTGCGCCC
GCGCTGGGACTTCCCCGGCGCCTTCTACTTCGTGGGCACCGTGGTGTCAACCATAGGTTT
CGGCATGACCACCCCCGCGACGGTGGGCGGGAAGGCCTTCCTCATCGCCTACGGGCTGTT
CGGCTGCGCTGGGACCATCCTGTTCTTCAACCTCTTCCTGGAGCGCATCATCTCGCTGCT
GGCCTTCATCATGCGCGCCTGCCGGGAGCGCCAGCTGCGCCGCAGCGGCCTGCTGCCCGC
CACCTTCCGCCGCGGCTCCGCGCTCTCGGAGGCCGACAGCCTGGCGGGCTGGAAGCCCTC
GGTGTACCACGTGCTGCTCATCCTGGGCCTGTTCGCCGTGCTGCTGTCCTGCTGCGCCTC
GGCCATGTACACCAGCGTGGAGGGTTGGGACTACGTGGACTCGCTCTACTTCTGCTTCGT
CACCTTCAGCACCATCGGCTTCGGGGACCTGGTGAGCAGCCAGCACGCCGCCTACCGGAA
CCAGGGGCTCTACCGCCTGGGCAACTTCCTCTTCATCCTGCTCGGCGTGTGCTGCATTTA
CTCGCTCTTCAACGTCATCTCCATCCTCATCAAGCAGGTGCTCAACTGGATGCTGCGCAA
GCTGAGCTGCCGCTGCTGCGCGCGCTGCTGCCCGGCTCCTGGCGCGCCCCTGGCCCGGCG
CAATGCCATCACCCCAGGCTCCCGGCTGCGCCGCCGCCTGGCCGCGCTCGGTGCCGACCC
CGCGGCCCGCGACAGCGACGCCGAGGGCCGCCGCCTCTCGGGCGAGCTCATCTCCATGCG
CGACCTCACGGCCTCCAACAAGGTGTCGCTGGCGCTGCTGCAGAAGCAGCTGTCGGAGAC
GGCCAACGGCTACCCGCGCAGCGTGTGCGTCAACACGCGCCAGAACGGCTTCTCGGGCGG
CGTGGGCGCGCTGGGCATCATGAACAACCGGCTGGCCGAGACCAGCGCCTCCAGGGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT