(Circular) (Six-base) MAPSORT of: pFN21AE5021.seq Check: 8646 from: 1 to: 5995 pFN21AE5021 5995 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2905 2905 Size: 5995 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 2749 4058 5442 5710 1632 Size: 592 525 1309 1384 268 1917 Fragments arranged by size: 1917 1384 1309 592 525 268 AfeI AGC'GCT Cuts at: 2245 2245 Size: 5995 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 4894 5871 1203 Size: 3691 977 1327 Fragments arranged by size: 3691 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 2381 2714 1166 Size: 1215 333 4447 Fragments arranged by size: 4447 1215 333 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2585 1184 Size: 1401 4594 AlwNI CAG_nnn'CTG Cuts at: 3738 5310 3738 Size: 1572 4423 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 3304 3530 3682 5791 1493 Size: 1811 226 152 2109 1697 Fragments arranged by size: 2109 1811 1697 226 152 AscI GG'CGCG_CC Cuts at: 2982 2982 Size: 5995 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 2183 3097 3232 3320 1870 Size: 73 240 914 135 88 4545 Fragments arranged by size: 4545 914 240 135 88 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2222 2357 2375 3050 Size: 325 217 627 375 60 135 18 675 Cuts at: 3050 3316 4771 618 Size: 266 1455 1842 Fragments arranged by size: 1842 1455 675 627 375 325 266 217 135 60 18 BanII G_rGCy'C Cuts at: 1391 2192 2829 3108 3314 1391 Size: 801 637 279 206 4072 Fragments arranged by size: 4072 801 637 279 206 BbeI G_GCGC'C Cuts at: 2226 2361 2226 Size: 135 5860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2164 928 Size: 971 265 4759 Fragments arranged by size: 4759 971 265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2306 2565 2625 Size: 107 122 71 631 605 634 259 60 Cuts at: 2625 3099 3225 4606 136 Size: 474 126 1381 1525 Fragments arranged by size: 1525 1381 634 631 605 474 259 126 122 107 71 60 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 2942 3412 2942 Size: 470 5525 Bme1580I G_kGCm'C Cuts at: 1790 2378 4056 5212 1790 Size: 588 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 588 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3127 3127 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 3274 4655 1239 Size: 1280 755 1381 2579 Fragments arranged by size: 2579 1381 1280 755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4120 4985 5283 4120 Size: 865 298 4832 Fragments arranged by size: 4832 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3272 4658 882 Size: 617 1773 1386 2219 Fragments arranged by size: 2219 1773 1386 617 BsaAI yAC'GTr Cuts at: 493 1204 2650 2734 3786 493 Size: 711 1446 84 1052 2702 Fragments arranged by size: 2702 1446 1052 711 84 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 2316 2358 3078 Size: 53 83 186 936 690 93 42 720 Cuts at: 3078 3802 4184 4819 275 Size: 724 382 635 1451 Fragments arranged by size: 1451 936 724 720 690 635 382 186 93 83 53 42 BsaWI w'CCGG_w Cuts at: 2814 4428 4826 5100 5247 5658 2814 Size: 1614 398 274 147 411 3151 Fragments arranged by size: 3151 1614 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2882 2912 1178 Size: 30 165 30 1479 30 4261 Fragments arranged by size: 4261 1479 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5995 BseYI C'CCAG_C Cuts at: 2217 2343 2468 3250 5198 5694 2217 Size: 126 125 782 1948 496 2518 Fragments arranged by size: 2518 1948 782 496 126 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 3445 4206 5234 1540 Size: 275 186 115 1329 761 1028 2301 Fragments arranged by size: 2301 1329 1028 761 275 186 115 BsiHKAI G_wGCw'C Cuts at: 2922 3108 4056 4141 5212 2922 Size: 186 948 85 1071 3705 Fragments arranged by size: 3705 1071 948 186 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 BsmBI CGTCTCn'nnnn_ Cuts at: 3170 3170 Size: 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2378 2829 2922 3108 3314 4056 Size: 399 402 186 451 93 186 206 742 Cuts at: 4056 4141 5212 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 402 399 206 186 186 93 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3258 3884 5614 3258 Size: 626 1730 3639 Fragments arranged by size: 3639 1730 626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2905 3350 844 Size: 2061 445 3489 Fragments arranged by size: 3489 2061 445 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3007 4490 4664 65 Size: 1183 1759 1483 174 1396 Fragments arranged by size: 1759 1483 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 2312 3267 4639 4826 1700 Size: 110 301 201 955 1372 187 2869 Fragments arranged by size: 2869 1372 955 301 201 187 110 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3385 BssHII G'CGCG_C Cuts at: 1925 2213 2275 2533 2958 2960 2982 3246 1925 Size: 288 62 258 425 2 22 264 4674 Fragments arranged by size: 4674 425 288 264 258 62 22 2 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 3204 794 Size: 2410 3585 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstYI r'GATC_y Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990 Size: 1353 367 385 17 1452 86 12 346 Cuts at: 5990 1972 Size: 1977 Fragments arranged by size: 1977 1452 1353 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2379 2590 3060 513 Size: 552 1314 211 470 3448 Fragments arranged by size: 3448 1314 552 470 211 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2428 487 Size: 683 56 1202 4054 Fragments arranged by size: 4054 1202 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DraIII CAC_nnn'GTG Cuts at: 2257 2257 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 2259 2307 Size: 54 1425 50 275 18 283 146 48 Cuts at: 2307 2700 3039 3180 3273 3442 4331 5960 8 Size: 393 339 141 93 169 889 1629 43 Fragments arranged by size: 1629 1425 889 393 339 283 275 169 146 141 93 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2113 3442 1537 Size: 275 301 1329 4090 Fragments arranged by size: 4090 1329 301 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2498 2855 2891 3925 1353 Size: 1145 357 36 1034 3423 Fragments arranged by size: 3423 1145 1034 357 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 2576 4582 4956 5102 387 Size: 1064 138 627 360 2006 374 146 1280 Fragments arranged by size: 2006 1280 1064 627 374 360 146 138 EcoICRI GAG'CTC Cuts at: 3106 3106 Size: 5995 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2224 2519 2749 3274 4058 4655 5442 Size: 393 592 295 230 525 784 597 787 Cuts at: 5442 5710 1239 Size: 268 1524 Fragments arranged by size: 1524 787 784 597 592 525 393 295 268 230 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2102 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 2785 2785 Size: 5995 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FspI TGC'GCA Cuts at: 2234 2250 2936 4501 2234 Size: 16 686 1565 3728 Fragments arranged by size: 3728 1565 686 16 HaeII r_GCGC'y Cuts at: 1678 2138 2183 2226 2247 2361 2551 3155 3288 Size: 460 45 43 21 114 190 604 133 Cuts at: 3288 5142 1678 Size: 1854 2531 Fragments arranged by size: 2531 1854 604 460 190 133 114 45 43 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373 Size: 32 135 32 3064 32 42 32 2626 Fragments arranged by size: 3064 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2388 3211 3339 3585 669 Size: 988 281 450 823 128 246 3079 Fragments arranged by size: 3079 988 823 450 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KasI G'GCGC_C Cuts at: 2222 2357 2222 Size: 135 5860 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3153 3159 5109 5293 1305 Size: 1848 6 1950 184 2007 Fragments arranged by size: 2007 1950 1848 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2714 3113 3953 4312 4471 518 Size: 648 1215 333 399 840 359 159 2042 Fragments arranged by size: 2042 1215 840 648 399 359 333 159 MspA1I CmG'CkG Cuts at: 1606 2554 2565 2592 2952 3062 3169 3411 4088 Size: 948 11 27 360 110 107 242 677 Cuts at: 4088 5236 5481 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 948 677 360 245 242 110 107 27 11 NaeI GCC'GGC Cuts at: 2314 2314 Size: 5995 NarI GG'CG_CC Cuts at: 2223 2358 2223 Size: 135 5860 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5443 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NgoMIV G'CCGG_C Cuts at: 2312 2312 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 3353 4898 1132 Size: 97 2124 1545 2229 Fragments arranged by size: 2229 2124 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5995 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5995 PfoI T'CCnGG_A Cuts at: 2496 2496 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PmlI CAC'GTG Cuts at: 2650 2650 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3215 3247 4064 4096 5577 5609 3215 Size: 32 817 32 1481 32 3601 Fragments arranged by size: 3601 1481 817 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 2785 Size: 5995 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PstI C_TGCA'G Cuts at: 830 3162 3347 830 Size: 2332 185 3478 Fragments arranged by size: 3478 2332 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2554 3169 2554 Size: 615 5380 SacI G_AGCT'C Cuts at: 3108 3108 Size: 5995 SacII CC_GC'GG Cuts at: 2593 3063 2593 Size: 470 5525 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SapI GCTCTTCn'nnn_ Cuts at: 2891 2891 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SexAI A'CCwGG_T Cuts at: 2151 2787 2151 Size: 636 5359 SfcI C'TryA_G Cuts at: 826 1045 3158 3343 4478 5159 5350 826 Size: 219 2113 185 1135 681 191 1471 Fragments arranged by size: 2113 1471 1135 681 219 191 185 SfiI GGCCn_nnn'nGGCC Cuts at: 2306 2306 Size: 5995 SfoI GGC'GCC Cuts at: 2224 2359 2224 Size: 135 5860 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 2312 1700 Size: 612 5383 SmaI CCC'GGG Cuts at: 1872 3322 1872 Size: 1450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 4099 5000 5262 820 Size: 197 926 240 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 240 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 2435 1517 Size: 918 5077 StyI C'CwwG_G Cuts at: 513 1065 3434 513 Size: 552 2369 3074 Fragments arranged by size: 3074 2369 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4022 4039 4192 4377 4822 325 Size: 1255 2442 17 153 185 445 1498 Fragments arranged by size: 2442 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 4241 5953 Size: 274 80 33 51 1443 729 1535 1712 Cuts at: 5953 96 Size: 138 Fragments arranged by size: 1712 1535 1443 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 2183 1943 Size: 240 5755 XmaI C'CCGG_G Cuts at: 1870 3320 1870 Size: 1450 4545 XmnI GAAnn'nnTTC Cuts at: 2435 3227 4124 2435 Size: 792 897 4306 Fragments arranged by size: 4306 897 792 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SanDI SrfI SwaI XcmI