Restriction map for
pFN21AE5021
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AseI AsnI Asp718I
AspEI AspI AvrII BalI BamHI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BseAI
BseRI BsgI BsiWI BsmI BspDI
BspEI BspLU11I BsrBI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DpnII
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HindIII HpaI KpnI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
Sau3AI SbfI ScaI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI TfiI Tru9I Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Aor51HI 238 AGCGCT AGC^GCT
AscI 976 GGCGCGCC GG^CGCGCC
BbrPI 643 CACGTG CAC^GTG
BbsI 166 GTCTTC GAAGAC(2/6)
BlpI 936 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 936 GCTGAGC GC^T[ACGT]AGC
BpuAI 166 GTCTTC GAAGAC(2/6)
BsaI 1273 GAGACC GGTCTC(1/5)
BsaWI 809 ACCGGA [AT]^CCGG[AT]
BsiEI 108 CGGCCG CG[GA][CT]^CG
BsmBI 1171 GAGACG CGTCTC(1/5)
Bsp1407I 701 TGTACA T^GTACA
BspHI 1253 TCATGA T^CATGA
BspMI 909 GCAGGT ACCTGC(4/8)
BsrDI 995 GCAATG GCAATG(2/0)
BsrGI 701 TGTACA T^GTACA
BsrI 920 ACTGG ACTGG(1/-1)
BstAPI 1193 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 936 GCTGAGC GC^T[ACGT]AGC
DdeI 937 CTGAG C^T[ACGT]AG
DraII 779 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 247 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 108 CGGCCG C^GGCCG
EclXI 108 CGGCCG C^GGCCG
Eco47III 238 AGCGCT AGC^GCT
Eco52I 108 CGGCCG C^GGCCG
EcoNI 93 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 779 GGGACCT [GA]G^G[ACGT]CC[CT]
HinfI 733 GACTC G^A[ACGT]TC
MaeIII 754 GTCAC ^GT[ACGT]AC
NaeI 307 GCCGGC GCC^GGC
NgoMIV 307 GCCGGC G^CCGGC
PaeR7I 178 CTCGAG C^TCGAG
PleI 733 GACTC GAGTC(4/5)
PmaCI 643 CACGTG CAC^GTG
PmlI 643 CACGTG CAC^GTG
PpuMI 779 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1153 CTGCAG CTGCA^G
RcaI 1253 TCATGA T^CATGA
SacI 1099 GAGCTC GAGCT^C
SapI 879 GCTCTTC GCTCTTC(1/4)
SfcI 1153 CTGCAG C^T[GA][CT]AG
SfiI 294 GGCCGCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 306 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 178 CTCGAG C^T[CT][GA]AG
SspBI 701 TGTACA T^GTACA
StuI 428 AGGCCT AGG^CCT
TaqI 179 TCGA T^CGA
Tsp45I 754 GTCAC ^GT[GC]AC
TthHB8I 179 TCGA T^CGA
XhoI 178 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 639 GTAC GT^AC
702 GTAC
Asp700I 426 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1218 GAACGGCTTC
AspHI 913 GTGCTC G[AT]GC[AT]^C
1099 GAGCTC
AvaII 467 GGACC G^G[AT]CC
780 GGACC
BbeI 217 GGCGCC GGCGC^C
352 GGCGCC
BpmI 1284 CTCCAG CTGGAG(16/14)
493 CTGGAG
BsaAI 643 CACGTG [CT]AC^GT[GA]
727 TACGTG
BsiHKAI 913 GTGCTC G[AT]GC[AT]^C
1099 GAGCTC
BsmAI 1171 GAGAC GTCTC(1/5)
1273 GAGAC
HincII 381 GTCAAC GT[CT]^[GA]AC
1204 GTCAAC
HindII 381 GTCAAC GT[CT]^[GA]AC
1204 GTCAAC
KasI 217 GGCGCC G^GCGCC
352 GGCGCC
KspI 585 CCGCGG CCGC^GG
1055 CCGCGG
NarI 217 GGCGCC GG^CGCC
352 GGCGCC
PvuII 547 CAGCTG CAG^CTG
1162 CAGCTG
RsaI 639 GTAC GT^AC
702 GTAC
SacII 585 CCGCGG CCGC^GG
1055 CCGCGG
SexAI 146 ACCTGGT A^CC[AT]GGT
782 ACCTGGT
SfoI 217 GGCGCC GGC^GCC
352 GGCGCC
XmnI 426 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1218 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AviII BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DsaI
EaeI EarI EcoRII FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI