(Linear) (Six-base) MAPSORT of: KIEE5021.seq Check: 5587 from: 1 to: 1290 KIEE5021.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 900 1290 Size: 900 390 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 219 744 1290 Size: 219 525 546 Fragments arranged by size: 546 525 219 AfeI AGC'GCT Cuts at: 0 240 1290 Size: 240 1050 AleI CACnn'nnGTG Cuts at: 0 376 709 1290 Size: 376 333 581 Fragments arranged by size: 581 376 333 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 580 1290 Size: 580 710 AscI GG'CGCG_CC Cuts at: 0 977 1290 Size: 977 313 AvaI C'yCGr_G Cuts at: 0 178 1092 1227 1290 Size: 178 914 135 63 Fragments arranged by size: 914 178 135 63 BanI G'GyrC_C Cuts at: 0 157 217 352 370 1045 1290 Size: 157 60 135 18 675 245 Fragments arranged by size: 675 245 157 135 60 18 BanII G_rGCy'C Cuts at: 0 187 824 1103 1290 Size: 187 637 279 187 Fragments arranged by size: 637 279 187 187 BbeI G_GCGC'C Cuts at: 0 221 356 1290 Size: 221 135 934 Fragments arranged by size: 934 221 135 BbsI GAAGACnn'nnnn_ Cuts at: 0 159 1290 Size: 159 1131 BglI GCCn_nnn'nGGC Cuts at: 0 301 560 620 1094 1220 1290 Size: 301 259 60 474 126 70 Fragments arranged by size: 474 301 259 126 70 60 BlpI GC'TnA_GC Cuts at: 0 937 1290 Size: 937 353 Bme1580I G_kGCm'C Cuts at: 0 373 1290 Size: 373 917 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1122 1290 Size: 1122 168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 514 1269 1290 Size: 514 755 21 Fragments arranged by size: 755 514 21 BsaI GGTCTCn'nnnn_ Cuts at: 0 1267 1290 Size: 1267 23 BsaAI yAC'GTr Cuts at: 0 645 729 1290 Size: 645 84 561 Fragments arranged by size: 645 561 84 BsaHI Gr'CG_yC Cuts at: 0 218 311 353 1073 1290 Size: 218 93 42 720 217 Fragments arranged by size: 720 218 217 93 42 BsaWI w'CCGG_w Cuts at: 0 809 1290 Size: 809 481 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 877 907 1290 Size: 877 30 383 Fragments arranged by size: 877 383 30 BseYI C'CCAG_C Cuts at: 0 212 338 463 1245 1290 Size: 212 126 125 782 45 Fragments arranged by size: 782 212 126 125 45 BsiEI CG_ry'CG Cuts at: 0 111 1290 Size: 111 1179 BsiHKAI G_wGCw'C Cuts at: 0 917 1103 1290 Size: 917 186 187 Fragments arranged by size: 917 187 186 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1165 1290 Size: 1165 125 Bsp1286I G_dGCh'C Cuts at: 0 187 373 824 917 1103 1290 Size: 187 186 451 93 186 187 Fragments arranged by size: 451 187 187 186 186 93 BspHI T'CATG_A Cuts at: 0 1253 1290 Size: 1253 37 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 900 1290 Size: 900 390 BsrDI GCAATG_nn' Cuts at: 0 1002 1290 Size: 1002 288 BsrFI r'CCGG_y Cuts at: 0 106 307 1262 1290 Size: 106 201 955 28 Fragments arranged by size: 955 201 106 28 BsrGI T'GTAC_A Cuts at: 0 701 1290 Size: 701 589 BssHII G'CGCG_C Cuts at: 0 208 270 528 953 955 977 1241 1290 Size: 208 62 258 425 2 22 264 49 Fragments arranged by size: 425 264 258 208 62 49 22 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1199 1290 Size: 1199 91 BtgI C'CryG_G Cuts at: 0 374 585 1055 1290 Size: 374 211 470 235 Fragments arranged by size: 470 374 235 211 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 423 1290 Size: 423 867 DraIII CAC_nnn'GTG Cuts at: 0 252 1290 Size: 252 1038 EaeI y'GGCC_r Cuts at: 0 108 254 302 695 1034 1175 1268 1290 Size: 108 146 48 393 339 141 93 22 Fragments arranged by size: 393 339 146 141 108 93 48 22 EagI C'GGCC_G Cuts at: 0 108 1290 Size: 108 1182 EarI CTCTTCn'nnn_ Cuts at: 0 493 850 886 1290 Size: 493 357 36 404 Fragments arranged by size: 493 404 357 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 211 571 1290 Size: 211 360 719 Fragments arranged by size: 719 360 211 EcoICRI GAG'CTC Cuts at: 0 1101 1290 Size: 1101 189 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 219 514 744 1269 1290 Size: 219 295 230 525 21 Fragments arranged by size: 525 295 230 219 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 97 1290 Size: 97 1193 EcoO109I rG'GnC_Cy Cuts at: 0 780 1290 Size: 780 510 FspI TGC'GCA Cuts at: 0 229 245 931 1290 Size: 229 16 686 359 Fragments arranged by size: 686 359 229 16 HaeII r_GCGC'y Cuts at: 0 133 178 221 242 356 546 1150 1283 Size: 133 45 43 21 114 190 604 133 Cuts at: 1283 1290 Size: 7 Fragments arranged by size: 604 190 133 133 114 45 43 21 7 HincII GTy'rAC Cuts at: 0 383 1206 1290 Size: 383 823 84 Fragments arranged by size: 823 383 84 KasI G'GCGC_C Cuts at: 0 217 352 1290 Size: 217 135 938 Fragments arranged by size: 938 217 135 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1148 1154 1290 Size: 1148 6 136 Fragments arranged by size: 1148 136 6 MslI CAynn'nnrTG Cuts at: 0 376 709 1108 1290 Size: 376 333 399 182 Fragments arranged by size: 399 376 333 182 MspA1I CmG'CkG Cuts at: 0 549 560 587 947 1057 1164 1290 Size: 549 11 27 360 110 107 126 Fragments arranged by size: 549 360 126 110 107 27 11 NaeI GCC'GGC Cuts at: 0 309 1290 Size: 309 981 NarI GG'CG_CC Cuts at: 0 218 353 1290 Size: 218 135 937 Fragments arranged by size: 937 218 135 NgoMIV G'CCGG_C Cuts at: 0 307 1290 Size: 307 983 PfoI T'CCnGG_A Cuts at: 0 491 1290 Size: 491 799 PmlI CAC'GTG Cuts at: 0 645 1290 Size: 645 645 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1210 1242 1290 Size: 1210 32 48 Fragments arranged by size: 1210 48 32 PpuMI rG'GwC_Cy Cuts at: 0 780 1290 Size: 780 510 PstI C_TGCA'G Cuts at: 0 1157 1290 Size: 1157 133 PvuII CAG'CTG Cuts at: 0 549 1164 1290 Size: 549 615 126 Fragments arranged by size: 615 549 126 SacI G_AGCT'C Cuts at: 0 1103 1290 Size: 1103 187 SacII CC_GC'GG Cuts at: 0 588 1058 1290 Size: 588 470 232 Fragments arranged by size: 588 470 232 SapI GCTCTTCn'nnn_ Cuts at: 0 886 1290 Size: 886 404 SexAI A'CCwGG_T Cuts at: 0 146 782 1290 Size: 146 636 508 Fragments arranged by size: 636 508 146 SfcI C'TryA_G Cuts at: 0 1153 1290 Size: 1153 137 SfiI GGCCn_nnn'nGGCC Cuts at: 0 301 1290 Size: 301 989 SfoI GGC'GCC Cuts at: 0 219 354 1290 Size: 219 135 936 Fragments arranged by size: 936 219 135 SgrAI Cr'CCGG_yG Cuts at: 0 307 1290 Size: 307 983 SmlI C'TyrA_G Cuts at: 0 178 1290 Size: 178 1112 StuI AGG'CCT Cuts at: 0 430 1290 Size: 430 860 TatI w'GTAC_w Cuts at: 0 701 1290 Size: 701 589 XhoI C'TCGA_G Cuts at: 0 178 1290 Size: 178 1112 XmnI GAAnn'nnTTC Cuts at: 0 430 1222 1290 Size: 430 792 68 Fragments arranged by size: 792 430 68 Enzymes that do cut: AarI AcuI AfeI AleI AlfI AscI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BtgI BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII SapI SexAI SfcI SfiI SfoI SgrAI SmlI StuI TatI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BseRI BsgI BsiWI BsmI BspEI BsrBI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SgfI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TstI Tth111I XbaI XcmI XmaI ZraI