RALGPS2 ORF clone pFN21AE4148

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4148

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AE4148 Sequence (with vector) Restriction Map

Product ID

FHC09649

ORF Sequence

DNA sequence (1749 bp)
Protein sequence (583 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4148

Symbol

RALGPS2

Alias : dJ595C2.1

Description

Ral GEF with PH domain and SH3 binding motif 2, transcript variant 10

Original Clone ID

cp94148

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1749 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 583 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1325.1 5.3e-213 100.0 RALGPS2 CCDS65733.1 1e-148 95.5 RALGPS2 CCDS35143.1 1e-94 62.0 RALGPS1 CCDS55346.1 3.7e-83 56.5 RALGPS1 CCDS55345.1 3.7e-83 57.2 RALGPS1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001895 47 219 PF00617 Guanine-nucleotide dissociation stimulator CDC25 HMMSmart IPR001895 45 288 SM00147 Guanine-nucleotide dissociation stimulator CDC25 IPR001849 458 571 SM00233 Pleckstrin homology domain ProfileScan IPR001895 49 287 PS50009 Guanine-nucleotide dissociation stimulator CDC25 IPR001849 457 569 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001309250 584 614444 63.1 99.3 XP_011517535 585 614444 62.9 99.3 NP_001309251 576 614444 62.4 99.3 NP_001309254 607 614444 62.7 97.8 XP_016870835 549 614444 61.3 99.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4148 Download

>KIEE4148  1749 bp
ATGGACCTAATGAACGGGCAGGCAAGCAGTGTCAATATTGCAGCTACTGCTTCTGAGAAA
AGTAGCAGCTCTGAATCCTTAAGTGACAAAGGCTCTGAATTGAAGAAAAGCTTTGATGCT
GTGGTATTCGATGTTCTTAAGGTTACACCAGAAGAATATGCGGGTCAGATAACATTAATG
GATGTTCCAGTATTTAAAGCTATTCAACCAGATGAGCTTTCAAGTTGTGGATGGAATAAA
AAAGAAAAATATAGTTCTGCACCAAATGCAGTTGCCTTCACAAGAAGATTCAATCATGTA
AGCTTTTGGGTTGTTAGAGAGATTCTTCATGCTCAAACATTAAAAATTAGAGCAGAAGTT
TTGAGCCACTATATTAAAACTGCTAAGAAACTGTATGAGCTGAATAACCTTCATGCACTT
ATGGCAGTGGTTTCTGGCCTACAGAGTGCCCCAATTTTCAGGTTGACTAAAACATGGGCG
TTATTAAGTCGAAAAGACAAAACTACCTTTGAAAAATTAGAATATGTAATGAGTAAAGAA
GATAACTACAAAAGACTCAGAGACTATATAAGTAGCTTAAAGATGACACCTTGCATTCCC
TATTTAGGTATCTATTTGTCAGATTTAACATACATCGATTCAGCATACCCATCAACTGGC
AGCATTCTAGAAAATGAGCAAAGATCAAATTTAATGAATAATATCCTTCGAATAATTTCT
GATTTACAGCAGTCTTGTGAATATGATATTCCCATGTTGCCTCATGTCCAAAAATATCTC
AACTCTGTTCAGTATATAGAAGAACTACAAAAATTTGTGGAAGACGATAATTACAAGCTT
TCATTAAAGATAGAACCAGGGACAAGCACCCCACGTTCTGCTGCTTCCAGAGAAGATTTA
GTAGGTCCTGAAGTAGGAGCGTCTCCACAGAGTGGACGAAAAAGTGTGGCAGCTGAAGGA
GCCTTGCTCCCACAGACACCGCCATCCCCTCGGAATCTGATTCCACATGGACATAGGAAG
TGCCATAGTTTGGGTTATAATTTCATTCATAAAATGAACACAGCAGAATTTAAGAGTGCA
ACGTTTCCAAATGCAGGACCAAGACATCTGTTAGATGATAGCGTCATGGAGCCCCATGCG
CCATCTCGAGGCCAAGCTGAAAGTTCTACTCTTTCTAGTGGAATATCAATAGGTAGCAGC
GATGGTTCTGAACTAAGTGAAGAGACCTCATGGCCTGCTTTTGAAAGGAACAGATTATAC
CATTCTCTCGGCCCGGTGACAAGAGTGGCACGAAATGGCTATCGAAGTCACATGAAGGCC
AGCAGTTCTGCAGAATCAGAAGATTTGGCAGTACATTTATATCCAGGAGCTGTTACTATT
CAAGGTGTTCTCAGGAGAAAAACTTTGTTAAAAGAAGGCAAAAAGCCTACAGTAGCATCT
TGGACAAAATATTGGGCAGCTTTGTGTGGGACACAGCTTTTTTACTATGCTGCCAAATCT
CTAAAGGCTACCGAAAGAAAACATTTCAAATCAACATCCAATAAGAACGTATCTGTGATA
GGATGGATGGTGATGATGGCTGATGACCCTGAACATCCTGATCTCTTCCTGCTGACTGAC
TCTGAGAAAGGAAATTCGTACAAGTTTCAAGCTGGCAATAGAATGAATGCAATGTTATGG
TTTAAGCATTTGAGTGCAGCCTGCCAAAGTAACAAACAACAGGTTCCTACAAACTTGATG
ACTTTTGAG

ORF Protein

Cloned ORF protein sequence for pFN21AE4148 Download

>KIEE4148  583 aa
MDLMNGQASSVNIAATASEKSSSSESLSDKGSELKKSFDAVVFDVLKVTPEEYAGQITLM
DVPVFKAIQPDELSSCGWNKKEKYSSAPNAVAFTRRFNHVSFWVVREILHAQTLKIRAEV
LSHYIKTAKKLYELNNLHALMAVVSGLQSAPIFRLTKTWALLSRKDKTTFEKLEYVMSKE
DNYKRLRDYISSLKMTPCIPYLGIYLSDLTYIDSAYPSTGSILENEQRSNLMNNILRIIS
DLQQSCEYDIPMLPHVQKYLNSVQYIEELQKFVEDDNYKLSLKIEPGTSTPRSAASREDL
VGPEVGASPQSGRKSVAAEGALLPQTPPSPRNLIPHGHRKCHSLGYNFIHKMNTAEFKSA
TFPNAGPRHLLDDSVMEPHAPSRGQAESSTLSSGISIGSSDGSELSEETSWPAFERNRLY
HSLGPVTRVARNGYRSHMKASSSAESEDLAVHLYPGAVTIQGVLRRKTLLKEGKKPTVAS
WTKYWAALCGTQLFYYAAKSLKATERKHFKSTSNKNVSVIGWMVMMADDPEHPDLFLLTD
SEKGNSYKFQAGNRMNAMLWFKHLSAACQSNKQQVPTNLMTFE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4148 Download

>pFN21AE4148  6454 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACCTAATGAACGGGCAGGCAAGCAGTGTCAA
TATTGCAGCTACTGCTTCTGAGAAAAGTAGCAGCTCTGAATCCTTAAGTGACAAAGGCTC
TGAATTGAAGAAAAGCTTTGATGCTGTGGTATTCGATGTTCTTAAGGTTACACCAGAAGA
ATATGCGGGTCAGATAACATTAATGGATGTTCCAGTATTTAAAGCTATTCAACCAGATGA
GCTTTCAAGTTGTGGATGGAATAAAAAAGAAAAATATAGTTCTGCACCAAATGCAGTTGC
CTTCACAAGAAGATTCAATCATGTAAGCTTTTGGGTTGTTAGAGAGATTCTTCATGCTCA
AACATTAAAAATTAGAGCAGAAGTTTTGAGCCACTATATTAAAACTGCTAAGAAACTGTA
TGAGCTGAATAACCTTCATGCACTTATGGCAGTGGTTTCTGGCCTACAGAGTGCCCCAAT
TTTCAGGTTGACTAAAACATGGGCGTTATTAAGTCGAAAAGACAAAACTACCTTTGAAAA
ATTAGAATATGTAATGAGTAAAGAAGATAACTACAAAAGACTCAGAGACTATATAAGTAG
CTTAAAGATGACACCTTGCATTCCCTATTTAGGTATCTATTTGTCAGATTTAACATACAT
CGATTCAGCATACCCATCAACTGGCAGCATTCTAGAAAATGAGCAAAGATCAAATTTAAT
GAATAATATCCTTCGAATAATTTCTGATTTACAGCAGTCTTGTGAATATGATATTCCCAT
GTTGCCTCATGTCCAAAAATATCTCAACTCTGTTCAGTATATAGAAGAACTACAAAAATT
TGTGGAAGACGATAATTACAAGCTTTCATTAAAGATAGAACCAGGGACAAGCACCCCACG
TTCTGCTGCTTCCAGAGAAGATTTAGTAGGTCCTGAAGTAGGAGCGTCTCCACAGAGTGG
ACGAAAAAGTGTGGCAGCTGAAGGAGCCTTGCTCCCACAGACACCGCCATCCCCTCGGAA
TCTGATTCCACATGGACATAGGAAGTGCCATAGTTTGGGTTATAATTTCATTCATAAAAT
GAACACAGCAGAATTTAAGAGTGCAACGTTTCCAAATGCAGGACCAAGACATCTGTTAGA
TGATAGCGTCATGGAGCCCCATGCGCCATCTCGAGGCCAAGCTGAAAGTTCTACTCTTTC
TAGTGGAATATCAATAGGTAGCAGCGATGGTTCTGAACTAAGTGAAGAGACCTCATGGCC
TGCTTTTGAAAGGAACAGATTATACCATTCTCTCGGCCCGGTGACAAGAGTGGCACGAAA
TGGCTATCGAAGTCACATGAAGGCCAGCAGTTCTGCAGAATCAGAAGATTTGGCAGTACA
TTTATATCCAGGAGCTGTTACTATTCAAGGTGTTCTCAGGAGAAAAACTTTGTTAAAAGA
AGGCAAAAAGCCTACAGTAGCATCTTGGACAAAATATTGGGCAGCTTTGTGTGGGACACA
GCTTTTTTACTATGCTGCCAAATCTCTAAAGGCTACCGAAAGAAAACATTTCAAATCAAC
ATCCAATAAGAACGTATCTGTGATAGGATGGATGGTGATGATGGCTGATGACCCTGAACA
TCCTGATCTCTTCCTGCTGACTGACTCTGAGAAAGGAAATTCGTACAAGTTTCAAGCTGG
CAATAGAATGAATGCAATGTTATGGTTTAAGCATTTGAGTGCAGCCTGCCAAAGTAACAA
ACAACAGGTTCCTACAAACTTGATGACTTTTGAGGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT