Restriction map for
pFN21AE4148
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I CpoI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 1080 AACGTT AA^CGTT
AseI 174 ATTAAT AT^TAAT
AsnI 174 ATTAAT AT^TAAT
Asp700I 710 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1145 CTCGAG C^[CT]CG[GA]G
BanII 1129 GAGCCC G[GA]GC[CT]^C
BbsI 820 GAAGAC GAAGAC(2/6)
BcnI 1272 CCCGG CC^[GC]GG
BpuAI 820 GAAGAC GAAGAC(2/6)
BsaI 1222 GAGACC GGTCTC(1/5)
BsmBI 920 CGTCTC CGTCTC(1/5)
BsoBI 1145 CTCGAG C^[CT]CG[GA]G
BspDI 634 ATCGAT AT^CGAT
BsrDI 1669 GCAATG GCAATG(2/0)
BstBI 707 TTCGAA TT^CGAA
CfoI 1138 GCGC GCG^C
ClaI 634 ATCGAT AT^CGAT
DraI 193 TTTAAA TTT^AAA
DraII 903 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 926 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 907 CCTGAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 903 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 160 GCGGG CCCGC(4/6)
HapII 1273 CCGG C^CGG
HhaI 1138 GCGC GCG^C
HinP1I 1138 GCGC G^CGC
HincII 462 GTTGAC GT[CT]^[GA]AC
HindII 462 GTTGAC GT[CT]^[GA]AC
HpaII 1273 CCGG C^CGG
MslI 1687 CATTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 950 CAGCTG C[AC]G^C[GT]G
MspI 1273 CCGG C^CGG
NciI 1272 CCCGG CC^[GC]GG
NspV 707 TTCGAA TT^CGAA
PaeR7I 1145 CTCGAG C^TCGAG
PpuMI 903 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 174 ATTAAT AT^TAAT
Psp1406I 1080 AACGTT AA^CGTT
PstI 1328 CTGCAG CTGCA^G
PvuII 950 CAGCTG CAG^CTG
SfuI 707 TTCGAA TT^CGAA
XbaI 666 TCTAGA T^CTAGA
XhoI 1145 CTCGAG C^TCGAG
XmnI 710 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 979 CCGC CCGC(-3/-1)
160 GCGG
AfaI 1351 GTAC GT^AC
1638 GTAC
AflII 78 CTTAAG C^TTAAG
136 CTTAAG
AlwNI 41 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
1364 CAGGAGCTG
BfaI 667 CTAG C^TAG
1175 CTAG
BfrI 78 CTTAAG C^TTAAG
136 CTTAAG
BmyI 446 GTGCCC G[AGT]GC[ACT]^C
1129 GAGCCC
BsaJI 856 CCAGGG C^C[ACGT][ACGT]GG
988 CCTCGG
BsgI 257 CTGCAC GTGCAG(16/14)
1694 GTGCAG
BsmFI 859 GGGAC GGGAC(10/14)
1468 GGGAC
Bsp1286I 446 GTGCCC G[AGT]GC[ACT]^C
1129 GAGCCC
BsrI 655 ACTGG ACTGG(1/-1)
187 CCAGT
BstAPI 40 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
259 GCACCAAATGC
BstNI 856 CCAGG CC^[AT]GG
1363 CCAGG
DpnII 683 GATC ^GATC
1600 GATC
EarI 1603 CTCTTC CTCTTC(1/4)
1219 GAAGAG
EcoRII 856 CCAGG ^CC[AT]GG
1363 CCAGG
HgaI 919 GCGTC GACGC(5/10)
1121 GCGTC
HphI 1275 GGTGA GGTGA(8/7)
1569 GGTGA
Ksp632I 1603 CTCTTC CTCTTC(1/4)
1219 GAAGAG
MaeI 667 CTAG C^TAG
1175 CTAG
MboI 683 GATC ^GATC
1600 GATC
MvaI 856 CCAGG CC^[AT]GG
1363 CCAGG
NdeII 683 GATC ^GATC
1600 GATC
PleI 554 GACTC GAGTC(4/5)
1618 GACTC
RsaI 1351 GTAC GT^AC
1638 GTAC
Sau3AI 683 GATC ^GATC
1600 GATC
SfaNI 115 GATGC GCATC(5/9)
1435 GCATC
SspI 34 AATATT AAT^ATT
1448 AATATT
TspRI 27 CAGTG CA[GC]TG[ACGT][ACGT]^
425 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BsiYI BslI BsmAI BsmI BssKI
BstF5I Cac8I Cfr13I DdeI Fnu4HI
FokI HaeIII HindIII HinfI ItaI
MaeII MaeIII MboII MnlI MseI
MwoI NlaIII NlaIV PflMI Sau96I
ScrFI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I
Van91I