(Circular) (Six-base) MAPSORT of: pFN21AE4148.seq Check: 5088 from: 1 to: 6454 pFN21AE4148 6454 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4264 278 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3797 1937 Size: 1860 4594 Acc65I G'GTAC_C Cuts at: 3775 3775 Size: 6454 AclI AA'CG_TT Cuts at: 1563 3086 4581 4954 1563 Size: 1523 1495 373 3063 Fragments arranged by size: 3063 1523 1495 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 2979 4517 5901 6169 1632 Size: 1302 45 1538 1384 268 1917 Fragments arranged by size: 1917 1538 1384 1302 268 45 AflII C'TTAA_G Cuts at: 820 1017 2083 2141 820 Size: 197 1066 58 5133 Fragments arranged by size: 5133 1066 197 58 AflIII A'CryG_T Cuts at: 1203 5353 6330 1203 Size: 4150 977 1327 Fragments arranged by size: 4150 1327 977 AgeI A'CCGG_T Cuts at: 5285 5285 Size: 6454 AhdI GACnn_n'nnGTC Cuts at: 5183 5183 Size: 6454 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3714 1184 Size: 2530 3924 AlwNI CAG_nnn'CTG Cuts at: 2051 3374 4197 5769 2051 Size: 1323 823 1572 2736 Fragments arranged by size: 2736 1572 1323 823 ApaLI G'TGCA_C Cuts at: 4511 5667 4511 Size: 1156 5298 ApoI r'AATT_y Cuts at: 1493 2692 2816 3071 3637 3763 3989 4141 6250 Size: 1199 124 255 566 126 226 152 2109 Cuts at: 6250 1493 Size: 1697 Fragments arranged by size: 2109 1697 1199 566 255 226 152 126 124 AseI AT'TA_AT Cuts at: 160 1032 2180 5008 160 Size: 872 1148 2828 1606 Fragments arranged by size: 2828 1606 1148 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 AvaI C'yCGr_G Cuts at: 1870 1943 3150 3779 1870 Size: 73 1207 629 4545 Fragments arranged by size: 4545 1207 629 73 BamHI G'GATC_C Cuts at: 3784 4151 3784 Size: 367 6087 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3775 5230 618 Size: 325 217 627 1988 1455 1842 Fragments arranged by size: 1988 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3138 3773 1391 Size: 1747 635 4072 Fragments arranged by size: 4072 1747 635 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2832 928 Size: 971 933 4550 Fragments arranged by size: 4550 971 933 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4645 4679 1903 Size: 34 2708 34 3678 Fragments arranged by size: 3678 2708 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4346 5556 4346 Size: 1210 5244 BclI T'GATC_A Cuts at: 1548 1548 Size: 6454 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5065 136 Size: 107 122 71 631 605 3393 1525 Fragments arranged by size: 3393 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6449 6449 Size: 6454 BlpI GC'TnA_GC Cuts at: 3871 3871 Size: 6454 Bme1580I G_kGCm'C Cuts at: 1790 2455 4515 5671 1790 Size: 665 2060 1156 2573 Fragments arranged by size: 2573 2060 1156 665 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4185 5143 442 Size: 416 53 83 399 24 2768 958 1753 Fragments arranged by size: 2768 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6454 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2987 2987 Size: 6454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5114 1239 Size: 3875 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4579 5444 5742 4579 Size: 865 298 5291 Fragments arranged by size: 5291 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3221 5117 882 Size: 617 1722 1896 2219 Fragments arranged by size: 2219 1896 1722 617 BsaAI yAC'GTr Cuts at: 493 1204 4245 493 Size: 711 3041 2702 Fragments arranged by size: 3041 2702 711 BsaBI GATnn'nnATC Cuts at: 6448 6448 Size: 6454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4261 4643 5278 275 Size: 53 83 186 936 2728 382 635 1451 Fragments arranged by size: 2728 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4887 5285 5559 5706 6117 4887 Size: 398 274 147 411 5224 Fragments arranged by size: 5224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6229 Fragments arranged by size: 6229 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6454 BseYI C'CCAG_C Cuts at: 5657 6153 5657 Size: 496 5958 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2247 3720 1163 Size: 573 511 1473 3897 Fragments arranged by size: 3897 1473 573 511 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3904 4665 5693 1540 Size: 275 186 1903 761 1028 2301 Fragments arranged by size: 2301 1903 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4515 4600 5671 4515 Size: 85 1071 5298 Fragments arranged by size: 5298 1071 85 BsmI GAATG_Cn' Cuts at: 2598 2667 3676 3965 4058 2598 Size: 69 1009 289 93 4994 Fragments arranged by size: 4994 1009 289 93 69 BsmBI CGTCTCn'nnnn_ Cuts at: 2931 2931 Size: 6454 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2455 3138 3773 4515 4600 5671 1391 Size: 399 665 683 635 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 683 665 635 399 85 BspEI T'CCGG_A Cuts at: 6117 6117 Size: 6454 BspHI T'CATG_A Cuts at: 4343 6073 4343 Size: 1730 4724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3809 844 Size: 2965 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4341 1394 Size: 429 2518 3507 Fragments arranged by size: 3507 2518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3681 4949 5123 65 Size: 1183 2433 1268 174 1396 Fragments arranged by size: 2433 1396 1268 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5098 5285 1700 Size: 110 3288 187 2869 Fragments arranged by size: 3288 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6454 BssSI C'ACGA_G Cuts at: 4514 5526 4514 Size: 1012 5442 BstAPI GCAn_nnn'nTGC Cuts at: 794 2051 2270 794 Size: 1257 219 4978 Fragments arranged by size: 4978 1257 219 BstBI TT'CG_AA Cuts at: 2713 5258 2713 Size: 2545 3909 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6454 BstYI r'GATC_y Cuts at: 1972 3784 4151 4536 4553 6005 6091 6103 6449 Size: 1812 367 385 17 1452 86 12 346 Cuts at: 6449 1972 Size: 1977 Fragments arranged by size: 1977 1812 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4963 Fragments arranged by size: 4963 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3219 487 Size: 683 56 1993 3722 Fragments arranged by size: 3722 1993 683 56 BtsI GCAGTG_nn' Cuts at: 2038 2436 3970 4764 4784 2038 Size: 398 1534 794 20 3708 Fragments arranged by size: 3708 1534 794 398 20 ClaI AT'CG_AT Cuts at: 1554 2640 1554 Size: 1086 5368 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6419 DraI TTT'AAA Cuts at: 2200 3758 2200 Size: 1558 4896 DraIII CAC_nnn'GTG Cuts at: 2936 2936 Size: 6454 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5461 809 Size: 507 4145 1802 Fragments arranged by size: 4145 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3901 4790 6419 Size: 54 1425 50 275 18 2071 889 1629 Cuts at: 6419 8 Size: 43 Fragments arranged by size: 2071 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3901 1537 Size: 275 2089 4090 Fragments arranged by size: 4090 2089 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3219 3614 4384 1353 Size: 1866 395 770 3423 Fragments arranged by size: 3423 1866 770 395 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5041 5415 5561 387 Size: 1064 138 3452 374 146 1280 Fragments arranged by size: 3452 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2934 2979 4517 5114 5901 6169 1239 Size: 393 1302 45 1538 597 787 268 1524 Fragments arranged by size: 1538 1524 1302 787 597 393 268 45 EcoNI CCTnn'n_nnAGG Cuts at: 2916 2916 Size: 6454 EcoO109I rG'GnC_Cy Cuts at: 2909 2909 Size: 6454 EcoRI G'AATT_C Cuts at: 3763 4141 3763 Size: 378 6076 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 6454 FspI TGC'GCA Cuts at: 4960 4960 Size: 6454 HaeII r_GCGC'y Cuts at: 1678 5601 1678 Size: 3923 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2672 2704 5095 Size: 32 135 32 509 32 559 32 2391 Cuts at: 5095 5127 5169 5201 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2391 559 509 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2469 3798 4044 669 Size: 988 281 531 1329 246 3079 Fragments arranged by size: 3079 1329 988 531 281 246 HindIII A'AGCT_T Cuts at: 748 2113 2305 2840 748 Size: 1365 192 535 4362 Fragments arranged by size: 4362 1365 535 192 HpaI GTT'AAC Cuts at: 4044 4044 Size: 6454 KpnI G_GTAC'C Cuts at: 3779 3779 Size: 6454 MfeI C'AATT_G Cuts at: 4053 4053 Size: 6454 MluI A'CGCG_T Cuts at: 6330 6330 Size: 6454 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5568 5752 1305 Size: 4263 184 2007 Fragments arranged by size: 4263 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4632 Fragments arranged by size: 4632 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3696 4412 4771 4930 518 Size: 648 2530 716 359 159 2042 Fragments arranged by size: 2530 2042 716 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2957 3870 4547 5695 5940 1606 Size: 1351 913 677 1148 245 2120 Fragments arranged by size: 2120 1351 1148 913 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4963 Fragments arranged by size: 4963 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6454 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6454 NotI GC'GGCC_GC Cuts at: 3901 3901 Size: 6454 NspI r_CATG'y Cuts at: 1132 1229 3812 5357 1132 Size: 97 2583 1545 2229 Fragments arranged by size: 2583 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6454 PciI A'CATG_T Cuts at: 5353 5353 Size: 6454 PflMI CCAn_nnn'nTGG Cuts at: 1108 2660 2936 3034 1108 Size: 1552 276 98 4528 Fragments arranged by size: 4528 1552 276 98 PfoI T'CCnGG_A Cuts at: 3367 3367 Size: 6454 PmeI GTTT'AAAC Cuts at: 3758 3758 Size: 6454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4523 4555 6036 6068 4523 Size: 32 1481 32 4909 Fragments arranged by size: 4909 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2909 2909 Size: 6454 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6454 PsiI TTA'TAA Cuts at: 3042 4024 3042 Size: 982 5472 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2121 2153 3245 3277 2121 Size: 32 1092 32 5298 Fragments arranged by size: 5298 1092 32 32 PstI C_TGCA'G Cuts at: 830 3337 3806 830 Size: 2507 469 3478 Fragments arranged by size: 3478 2507 469 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6454 PvuII CAG'CTG Cuts at: 2957 2957 Size: 6454 SalI G'TCGA_C Cuts at: 1936 3796 1936 Size: 1860 4594 SbfI CC_TGCA'GG Cuts at: 3806 3806 Size: 6454 ScaI AGT'ACT Cuts at: 4702 6414 4702 Size: 1712 4742 SfcI C'TryA_G Cuts at: 826 1045 2444 3333 3432 3802 4937 5618 5809 Size: 219 1399 889 99 370 1135 681 191 Cuts at: 5809 826 Size: 1471 Fragments arranged by size: 1471 1399 1135 889 681 370 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6454 SmaI CCC'GGG Cuts at: 1872 3781 1872 Size: 1909 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2083 2141 3150 4558 5459 5721 Size: 197 926 140 58 1009 1408 901 262 Cuts at: 5721 820 Size: 1553 Fragments arranged by size: 1553 1408 1009 926 901 262 197 140 58 SnaBI TAC'GTA Cuts at: 493 4245 493 Size: 3752 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6454 SphI G_CATG'C Cuts at: 1132 3812 1132 Size: 2680 3774 SspI AAT'ATT Cuts at: 5 52 2041 3455 4378 5 Size: 47 1989 1414 923 2081 Fragments arranged by size: 2081 1989 1414 923 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6454 StyI C'CwwG_G Cuts at: 513 1065 2004 3893 513 Size: 552 939 1889 3074 Fragments arranged by size: 3074 1889 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4481 4498 4651 4836 5281 325 Size: 1255 2901 17 153 185 445 1498 Fragments arranged by size: 2901 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3355 4700 6412 Size: 274 80 33 51 1443 1378 1345 1712 Cuts at: 6412 96 Size: 138 Fragments arranged by size: 1712 1443 1378 1345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3558 3590 6414 6446 3558 Size: 32 2824 32 3566 Fragments arranged by size: 3566 2824 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6454 XbaI T'CTAG_A Cuts at: 2671 3790 2671 Size: 1119 5335 XhoI C'TCGA_G Cuts at: 1943 3150 1943 Size: 1207 5247 XmaI C'CCGG_G Cuts at: 1870 3779 1870 Size: 1909 4545 XmnI GAAnn'nnTTC Cuts at: 2719 4583 2719 Size: 1864 4590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4262 276 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI