(Linear) (Six-base) MAPSORT of: KIEE4148.seq Check: 4307 from: 1 to: 1749 KIEE4148.orf With 182 enzymes: SgfI * July 3, 2012 18:39 .. AclI AA'CG_TT Cuts at: 0 1081 1749 Size: 1081 668 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 974 1749 Size: 929 45 775 Fragments arranged by size: 929 775 45 AflII C'TTAA_G Cuts at: 0 78 136 1749 Size: 78 58 1613 Fragments arranged by size: 1613 78 58 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1709 1749 Size: 1709 40 AlwNI CAG_nnn'CTG Cuts at: 0 46 1369 1749 Size: 46 1323 380 Fragments arranged by size: 1323 380 46 ApoI r'AATT_y Cuts at: 0 687 811 1066 1632 1749 Size: 687 124 255 566 117 Fragments arranged by size: 687 566 255 124 117 AseI AT'TA_AT Cuts at: 0 175 1749 Size: 175 1574 AvaI C'yCGr_G Cuts at: 0 1145 1749 Size: 1145 604 BanII G_rGCy'C Cuts at: 0 1133 1749 Size: 1133 616 BbsI GAAGACnn'nnnn_ Cuts at: 0 827 1749 Size: 827 922 Bme1580I G_kGCm'C Cuts at: 0 450 1749 Size: 450 1299 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 982 1749 Size: 982 767 BsaI GGTCTCn'nnnn_ Cuts at: 0 1216 1749 Size: 1216 533 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 242 1715 1749 Size: 242 1473 34 Fragments arranged by size: 1473 242 34 BsmI GAATG_Cn' Cuts at: 0 593 662 1671 1749 Size: 593 69 1009 78 Fragments arranged by size: 1009 593 78 69 BsmBI CGTCTCn'nnnn_ Cuts at: 0 926 1749 Size: 926 823 Bsp1286I G_dGCh'C Cuts at: 0 450 1133 1749 Size: 450 683 616 Fragments arranged by size: 683 616 450 BsrDI GCAATG_nn' Cuts at: 0 1676 1749 Size: 1676 73 BstAPI GCAn_nnn'nTGC Cuts at: 0 46 265 1749 Size: 46 219 1484 Fragments arranged by size: 1484 219 46 BstBI TT'CG_AA Cuts at: 0 708 1749 Size: 708 1041 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1214 1749 Size: 1214 535 BtsI GCAGTG_nn' Cuts at: 0 33 431 1749 Size: 33 398 1318 Fragments arranged by size: 1318 398 33 ClaI AT'CG_AT Cuts at: 0 635 1749 Size: 635 1114 DraI TTT'AAA Cuts at: 0 195 1749 Size: 195 1554 DraIII CAC_nnn'GTG Cuts at: 0 931 1749 Size: 931 818 EarI CTCTTCn'nnn_ Cuts at: 0 1214 1609 1749 Size: 1214 395 140 Fragments arranged by size: 1214 395 140 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 974 1749 Size: 929 45 775 Fragments arranged by size: 929 775 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 911 1749 Size: 911 838 EcoO109I rG'GnC_Cy Cuts at: 0 904 1749 Size: 904 845 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 126 1749 Size: 126 1623 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 76 108 667 699 1749 Size: 76 32 559 32 1050 Fragments arranged by size: 1050 559 76 32 32 HincII GTy'rAC Cuts at: 0 464 1749 Size: 464 1285 HindIII A'AGCT_T Cuts at: 0 108 300 835 1749 Size: 108 192 535 914 Fragments arranged by size: 914 535 192 108 MslI CAynn'nnrTG Cuts at: 0 1691 1749 Size: 1691 58 MspA1I CmG'CkG Cuts at: 0 952 1749 Size: 952 797 PflMI CCAn_nnn'nTGG Cuts at: 0 655 931 1029 1749 Size: 655 276 98 720 Fragments arranged by size: 720 655 276 98 PfoI T'CCnGG_A Cuts at: 0 1362 1749 Size: 1362 387 PpuMI rG'GwC_Cy Cuts at: 0 904 1749 Size: 904 845 PsiI TTA'TAA Cuts at: 0 1037 1749 Size: 1037 712 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 116 148 1240 1272 1749 Size: 116 32 1092 32 477 Fragments arranged by size: 1092 477 116 32 32 PstI C_TGCA'G Cuts at: 0 1332 1749 Size: 1332 417 PvuII CAG'CTG Cuts at: 0 952 1749 Size: 952 797 SfcI C'TryA_G Cuts at: 0 439 1328 1427 1749 Size: 439 889 99 322 Fragments arranged by size: 889 439 322 99 SmlI C'TyrA_G Cuts at: 0 78 136 1145 1749 Size: 78 58 1009 604 Fragments arranged by size: 1009 604 78 58 SspI AAT'ATT Cuts at: 0 36 1450 1749 Size: 36 1414 299 Fragments arranged by size: 1414 299 36 TatI w'GTAC_w Cuts at: 0 1350 1749 Size: 1350 399 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1553 1585 1749 Size: 1553 32 164 Fragments arranged by size: 1553 164 32 XbaI T'CTAG_A Cuts at: 0 666 1749 Size: 666 1083 XhoI C'TCGA_G Cuts at: 0 1145 1749 Size: 1145 604 XmnI GAAnn'nnTTC Cuts at: 0 714 1749 Size: 714 1035 Enzymes that do cut: AclI AcuI AflII AlfI AlwNI ApoI AseI AvaI BanII BbsI Bme1580I BplI BsaI BsgI BsmI BsmBI Bsp1286I BsrDI BstAPI BstBI BtgZI BtsI ClaI DraI DraIII EarI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MslI MspA1I PflMI PfoI PpuMI PsiI PsrI PstI PvuII SfcI SmlI SspI TatI TstI XbaI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgI CspCI DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII Tth111I XcmI XmaI ZraI