TRIM50 ORF clone pFN21AE4114

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4114

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AE4114 Sequence (with vector) Restriction Map

Product ID

FHC12585

ORF Sequence

DNA sequence (1461 bp)
Protein sequence (487 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4114

Symbol

TRIM50

Alias : TRIM50A

Description

tripartite motif containing 50, transcript variant 1

Original Clone ID

cp94114

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1461 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 487 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34654.1 2.2e-146 100.0 TRIM50 CCDS34665.1 2.8e-65 93.2 TRIM73 CCDS5545.1 7.8e-65 92.8 TRIM74 CCDS32437.1 5.8e-38 33.8 TRIM72 CCDS34378.1 8.7e-31 29.3 TRIM39 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003879 292 309 PR01407 Butyrophylin-like IPR003879 331 355 PR01407 Butyrophylin-like IPR003879 361 374 PR01407 Butyrophylin-like IPR003879 405 429 PR01407 Butyrophylin-like HMMPfam IPR018957 16 56 PF00097 Zinc finger IPR000315 85 125 PF00643 Zinc finger IPR003877 347 454 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR001841 16 56 SM00184 Zinc finger IPR000315 84 125 SM00336 Zinc finger IPR006574 293 345 SM00589 SPRY-associated IPR018355 346 474 SM00449 SPla/RYanodine receptor subgroup ProfileScan IPR001841 16 57 PS50089 Zinc finger IPR000315 84 125 PS50119 Zinc finger IPR001870 276 475 PS50188 B302/SPRY domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001268380 487 612548 100.0 100.0 NP_835226 487 612548 100.0 100.0 XP_011514091 486 612548 99.8 100.0 NP_001268379 486 612548 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4114 Download

>KIEE4114  1461 bp
ATGGCTTGGCAGGTGAGCCTGCCAGAGCTGGAGGACCGGCTTCAGTGTCCCATCTGCCTG
GAGGTCTTCAAGGAGCCCCTGATGCTGCAGTGTGGCCACTCTTACTGCAAGGGCTGCCTG
GTTTCCCTGTCCTGCCACCTGGATGCCGAGCTGCGCTGCCCCGTGTGCCGGCAGGCGGTG
GACGGCAGCAGCTCCCTGCCCAACGTCTCCCTGGCCAGGGTGATCGAAGCCCTGAGGCTC
CCTGGGGACCCGGAGCCCAAGGTCTGCGTGCACCACCGGAACCCGCTCAGCCTTTTCTGC
GAGAAGGACCAGGAGCTCATCTGTGGCCTCTGCGGTCTGCTGGGCTCCCACCAACACCAC
CCGGTCACGCCCGTCTCCACCGTCTACAGCCGCATGAAGGAGGAGCTCGCAGCCCTCATC
TCTGAGCTGAAGCAGGAGCAGAAAAAGGTGGATGAGCTCATCGCCAAACTGGTGAACAAC
CGGACCCGAATCGTCAATGAGTCGGATGTCTTCAGCTGGGTGATCCGCCGCGAGTTCCAG
GAGCTGCACCACCTGGTGGATGAGGAGAAGGCCCGCTGCCTGGAGGGGATAGGGGGTCAC
ACCCGTGGCCTGGTGGCCTCCCTGGACATGCAGCTGGAGCAGGCCCAGGGAACCCGGGAG
CGGCTGGCCCAAGCCGAGTGTGTGCTGGAACAGTTCGGCAATGAGGACCACCACAAGTTC
ATCCGGAAGTTCCACTCCATGGCCTCCAGAGCAGAGATGCCGCAGGCCCGGCCCTTAGAA
GGCGCATTCAGCCCCATCTCCTTCAAGCCAGGCCTCCACCAGGCTGACATCAAGCTGACC
GTGTGGAAAAGGCTCTTCCGGAAAGTTTTGCCAGCCCCGGAGCCTCTCAAGTTGGACCCT
GCCACTGCCCACCCACTCCTGGAGCTCTCCAAGGGCAACACGGTGGTGCAGTGCGGGCTT
CTGGCCCAGCGGCGAGCCAGCCAGCCTGAGCGCTTCGACTACAGCACCTGCGTCCTGGCC
AGCCGCGGCTTCTCCTGCGGCCGCCACTACTGGGAGGTGGTGGTGGGCAGCAAGAGCGAC
TGGCGCCTGGGGGTCATCAAGGGCACAGCCAGCCGTAAGGGCAAGCTGAACAGGTCCCCC
GAGCACGGCGTGTGGCTGATCGGCCTGAAGGAGGGCCGGGTGTACGAAGCCTTTGCCTGC
CCCCGGGTACCCCTGCCCGTGGCCGGCCACCCCCACCGCATCGGGCTCTACCTGCACTAT
GAGCAGGGCGAACTCACCTTCTTCGATGCCGACCGCCCCGATGACCTGCGGCCGCTCTAC
ACCTTCCAGGCCGACTTCCAGGGCAAGCTCTACCCCATCCTGGACACCTGCTGGCACGAG
AGGGGCAGCAACTCGCTGCCCATGGTGCTGCCCCCGCCCAGCGGGCCTGGCCCCCTCAGC
CCCGAGCAGCCCACCAAGCTG

ORF Protein

Cloned ORF protein sequence for pFN21AE4114 Download

>KIEE4114  487 aa
MAWQVSLPELEDRLQCPICLEVFKEPLMLQCGHSYCKGCLVSLSCHLDAELRCPVCRQAV
DGSSSLPNVSLARVIEALRLPGDPEPKVCVHHRNPLSLFCEKDQELICGLCGLLGSHQHH
PVTPVSTVYSRMKEELAALISELKQEQKKVDELIAKLVNNRTRIVNESDVFSWVIRREFQ
ELHHLVDEEKARCLEGIGGHTRGLVASLDMQLEQAQGTRERLAQAECVLEQFGNEDHHKF
IRKFHSMASRAEMPQARPLEGAFSPISFKPGLHQADIKLTVWKRLFRKVLPAPEPLKLDP
ATAHPLLELSKGNTVVQCGLLAQRRASQPERFDYSTCVLASRGFSCGRHYWEVVVGSKSD
WRLGVIKGTASRKGKLNRSPEHGVWLIGLKEGRVYEAFACPRVPLPVAGHPHRIGLYLHY
EQGELTFFDADRPDDLRPLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGPLS
PEQPTKL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4114 Download

>pFN21AE4114  6166 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTTGGCAGGTGAGCCTGCCAGAGCTGGAGGA
CCGGCTTCAGTGTCCCATCTGCCTGGAGGTCTTCAAGGAGCCCCTGATGCTGCAGTGTGG
CCACTCTTACTGCAAGGGCTGCCTGGTTTCCCTGTCCTGCCACCTGGATGCCGAGCTGCG
CTGCCCCGTGTGCCGGCAGGCGGTGGACGGCAGCAGCTCCCTGCCCAACGTCTCCCTGGC
CAGGGTGATCGAAGCCCTGAGGCTCCCTGGGGACCCGGAGCCCAAGGTCTGCGTGCACCA
CCGGAACCCGCTCAGCCTTTTCTGCGAGAAGGACCAGGAGCTCATCTGTGGCCTCTGCGG
TCTGCTGGGCTCCCACCAACACCACCCGGTCACGCCCGTCTCCACCGTCTACAGCCGCAT
GAAGGAGGAGCTCGCAGCCCTCATCTCTGAGCTGAAGCAGGAGCAGAAAAAGGTGGATGA
GCTCATCGCCAAACTGGTGAACAACCGGACCCGAATCGTCAATGAGTCGGATGTCTTCAG
CTGGGTGATCCGCCGCGAGTTCCAGGAGCTGCACCACCTGGTGGATGAGGAGAAGGCCCG
CTGCCTGGAGGGGATAGGGGGTCACACCCGTGGCCTGGTGGCCTCCCTGGACATGCAGCT
GGAGCAGGCCCAGGGAACCCGGGAGCGGCTGGCCCAAGCCGAGTGTGTGCTGGAACAGTT
CGGCAATGAGGACCACCACAAGTTCATCCGGAAGTTCCACTCCATGGCCTCCAGAGCAGA
GATGCCGCAGGCCCGGCCCTTAGAAGGCGCATTCAGCCCCATCTCCTTCAAGCCAGGCCT
CCACCAGGCTGACATCAAGCTGACCGTGTGGAAAAGGCTCTTCCGGAAAGTTTTGCCAGC
CCCGGAGCCTCTCAAGTTGGACCCTGCCACTGCCCACCCACTCCTGGAGCTCTCCAAGGG
CAACACGGTGGTGCAGTGCGGGCTTCTGGCCCAGCGGCGAGCCAGCCAGCCTGAGCGCTT
CGACTACAGCACCTGCGTCCTGGCCAGCCGCGGCTTCTCCTGCGGCCGCCACTACTGGGA
GGTGGTGGTGGGCAGCAAGAGCGACTGGCGCCTGGGGGTCATCAAGGGCACAGCCAGCCG
TAAGGGCAAGCTGAACAGGTCCCCCGAGCACGGCGTGTGGCTGATCGGCCTGAAGGAGGG
CCGGGTGTACGAAGCCTTTGCCTGCCCCCGGGTACCCCTGCCCGTGGCCGGCCACCCCCA
CCGCATCGGGCTCTACCTGCACTATGAGCAGGGCGAACTCACCTTCTTCGATGCCGACCG
CCCCGATGACCTGCGGCCGCTCTACACCTTCCAGGCCGACTTCCAGGGCAAGCTCTACCC
CATCCTGGACACCTGCTGGCACGAGAGGGGCAGCAACTCGCTGCCCATGGTGCTGCCCCC
GCCCAGCGGGCCTGGCCCCCTCAGCCCCGAGCAGCCCACCAAGCTGGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT