################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI MaeI MamI MfeI MflI MluI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1206 GGTACC G^GTACC AccI 382 GTCTAC GT^[AC][GT]AC AcyI 1082 GGCGCC G[GA]^CG[CT]C Alw44I 268 GTGCAC G^TGCAC AlwI 522 GATCC GGATC(4/5) AocI 231 CCTGAGG CC^T[ACGT]AGG Aor51HI 989 AGCGCT AGC^GCT ApaLI 268 GTGCAC G^TGCAC Asp718I 1206 GGTACC G^GTACC BbeI 1082 GGCGCC GGCGC^C BbvCI 1434 CCTCAGC CCTCAGC(-5/-2) BcgI 1185 CGAAGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 285 GCTCAGC GC^T[ACGT]AGC Bpu1102I 285 GCTCAGC GC^T[ACGT]AGC BsaHI 1082 GGCGCC G[GA]^CG[CT]C BsmI 784 GCATTC GAATGC(1/-1) BsrDI 698 GCAATG GCAATG(2/0) BssSI 1375 CACGAG CACGAG(-5/-1) BstAPI 107 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 50 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 231 CCTGAGG CC^T[ACGT]AGG CelII 285 GCTCAGC GC^T[ACGT]AGC EarI 853 CTCTTC CTCTTC(1/4) Eco47III 989 AGCGCT AGC^GCT Eco81I 231 CCTGAGG CC^T[ACGT]AGG FseI 1221 GGCCGGCC GGCCGG^CC HgaI 1010 GCGTC GACGC(5/10) Hin1I 1082 GGCGCC G[GA]^CG[CT]C KasI 1082 GGCGCC G^GCGCC KpnI 1206 GGTACC GGTAC^C Ksp632I 853 CTCTTC CTCTTC(1/4) KspI 1023 CCGCGG CCGC^GG MaeII 203 ACGT A^CGT NarI 1082 GGCGCC GG^CGCC NspI 626 ACATGC [GA]CATG^[CT] PleI 499 GAGTC GAGTC(4/5) PstI 85 CTGCAG CTGCA^G SacII 1023 CCGCGG CCGC^GG SapI 852 GCTCTTC GCTCTTC(1/4) SexAI 551 ACCTGGT A^CC[AT]GGT SfoI 1082 GGCGCC GGC^GCC SmlI 886 CTCAAG C^T[CT][GA]AG StuI 810 AGGCCT AGG^CCT TfiI 488 GAATC G^A[AT]TC XcmI 200 CCAACGTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 529 CGCG CG^CG 1024 CGCG AccIII 722 TCCGGA T^CCGGA 857 TCCGGA AfaI 1182 GTAC GT^AC 1207 GTAC AlwNI 538 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1002 CAGCACCTG BanI 1082 GGCGCC G^G[CT][GA]CC 1206 GGTACC BbsI 64 GTCTTC GAAGAC(2/6) 508 GTCTTC BglI 1112 GCCGTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1416 GCCCAGCGGGC BpuAI 64 GTCTTC GAAGAC(2/6) 508 GTCTTC BseAI 722 TCCGGA T^CCGGA 857 TCCGGA BseRI 400 GAGGAG GAGGAG(10/8) 562 GAGGAG BsmAI 205 GTCTC GTCTC(1/5) 373 GTCTC BsmBI 204 CGTCTC CGTCTC(1/5) 372 CGTCTC BspEI 722 TCCGGA T^CCGGA 857 TCCGGA BstUI 529 CGCG CG^CG 1024 CGCG DraII 245 GGGACCC [GA]G^G[ACGT]CC[CT] 1132 AGGTCCC DraIII 550 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1144 CACGGCGTG EagI 1038 CGGCCG C^GGCCG 1309 CGGCCG EclXI 1038 CGGCCG C^GGCCG 1309 CGGCCG Eco52I 1038 CGGCCG C^GGCCG 1309 CGGCCG EcoNI 813 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1164 CCTGAAGGAGG EcoO109I 245 GGGACCC [GA]G^G[ACGT]CC[CT] 1132 AGGTCCC HaeII 989 AGCGCT [GA]GCGC^[CT] 1082 GGCGCC HinfI 488 GAATC G^A[ACGT]TC 499 GAGTC MaeIII 364 GTCAC ^GT[ACGT]AC 596 GTCAC MroI 722 TCCGGA T^CCGGA 857 TCCGGA MvnI 529 CGCG CG^CG 1024 CGCG NaeI 167 GCCGGC GCC^GGC 1222 GCCGGC NcoI 737 CCATGG C^CATGG 1400 CCATGG NgoMIV 167 GCCGGC G^CCGGC 1222 GCCGGC NotI 1037 GCGGCCGC GC^GGCCGC 1308 GCGGCCGC PflMI 549 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 732 CCACTCCATGG PpuMI 245 GGGACCC [GA]G^G[AT]CC[CT] 1132 AGGTCCC PvuII 513 CAGCTG CAG^CTG 631 CAGCTG RsaI 1182 GTAC GT^AC 1207 GTAC SmaI 653 CCCGGG CCC^GGG 1202 CCCGGG Tsp45I 364 GTCAC ^GT[GC]AC 596 GTCAC Van91I 549 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 732 CCACTCCATGG XmaCI 653 CCCGGG C^CCGGG 1202 CCCGGG XmaI 653 CCCGGG C^CCGGG 1202 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AluI AspHI AvaI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaJI BsaWI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI TspRI TthHB8I