(Linear) (Six-base) MAPSORT of: KIEE4114.seq Check: 7629 from: 1 to: 1461 KIEE4114.orf With 182 enzymes: SgfI * July 3, 2012 18:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1015 1375 1461 Size: 1015 360 86 Fragments arranged by size: 1015 360 86 AccI GT'mk_AC Cuts at: 0 383 1461 Size: 383 1078 Acc65I G'GTAC_C Cuts at: 0 1206 1461 Size: 1206 255 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 448 495 1186 1461 Size: 25 423 47 691 275 Fragments arranged by size: 691 423 275 47 25 AfeI AGC'GCT Cuts at: 0 991 1461 Size: 991 470 AleI CACnn'nnGTG Cuts at: 0 602 943 1461 Size: 602 341 518 Fragments arranged by size: 602 518 341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 211 1411 1461 Size: 211 1200 50 Fragments arranged by size: 1200 211 50 AlwNI CAG_nnn'CTG Cuts at: 0 543 1007 1461 Size: 543 464 454 Fragments arranged by size: 543 464 454 ApaLI G'TGCA_C Cuts at: 0 268 1461 Size: 268 1193 AvaI C'yCGr_G Cuts at: 0 653 1138 1202 1441 1461 Size: 653 485 64 239 20 Fragments arranged by size: 653 485 239 64 20 BanI G'GyrC_C Cuts at: 0 1082 1206 1461 Size: 1082 124 255 Fragments arranged by size: 1082 255 124 BanII G_rGCy'C Cuts at: 0 77 257 317 346 407 458 926 1247 Size: 77 180 60 29 61 51 468 321 Cuts at: 1247 1461 Size: 214 Fragments arranged by size: 468 321 214 180 77 61 60 51 29 BbeI G_GCGC'C Cuts at: 0 1086 1461 Size: 1086 375 BbsI GAAGACnn'nnnn_ Cuts at: 0 57 501 1461 Size: 57 444 960 Fragments arranged by size: 960 444 57 BbvCI CC'TCA_GC Cuts at: 0 1435 1461 Size: 1435 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1174 1208 1461 Size: 1174 34 253 Fragments arranged by size: 1174 253 34 BglI GCCn_nnn'nGGC Cuts at: 0 1118 1422 1461 Size: 1118 304 39 Fragments arranged by size: 1118 304 39 BlpI GC'TnA_GC Cuts at: 0 286 1461 Size: 286 1175 Bme1580I G_kGCm'C Cuts at: 0 272 1105 1461 Size: 272 833 356 Fragments arranged by size: 833 356 272 BmrI ACTGGGnnnn_n' Cuts at: 0 1059 1461 Size: 1059 402 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 79 601 655 729 940 1461 Size: 49 30 522 54 74 211 521 Fragments arranged by size: 522 521 211 74 54 49 30 Bpu10I CC'TnA_GC Cuts at: 0 986 1435 1461 Size: 986 449 26 Fragments arranged by size: 986 449 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 871 1461 Size: 871 590 BsaHI Gr'CG_yC Cuts at: 0 1083 1461 Size: 1083 378 BsaWI w'CCGG_w Cuts at: 0 275 479 722 857 1461 Size: 275 204 243 135 604 Fragments arranged by size: 604 275 243 204 135 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 577 1461 Size: 415 162 884 Fragments arranged by size: 884 415 162 BseYI C'CCAG_C Cuts at: 0 339 515 965 1417 1461 Size: 339 176 450 452 44 Fragments arranged by size: 452 450 339 176 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 967 1237 1461 Size: 529 438 270 224 Fragments arranged by size: 529 438 270 224 BsiEI CG_ry'CG Cuts at: 0 1041 1293 1312 1461 Size: 1041 252 19 149 Fragments arranged by size: 1041 252 149 19 BsiHKAI G_wGCw'C Cuts at: 0 272 317 407 458 926 1145 1461 Size: 272 45 90 51 468 219 316 Fragments arranged by size: 468 316 272 219 90 51 45 BsmI GAATG_Cn' Cuts at: 0 784 1461 Size: 784 677 BsmBI CGTCTCn'nnnn_ Cuts at: 0 210 378 1461 Size: 210 168 1083 Fragments arranged by size: 1083 210 168 Bsp1286I G_dGCh'C Cuts at: 0 77 257 272 317 346 407 458 926 Size: 77 180 15 45 29 61 51 468 Cuts at: 926 1105 1145 1247 1461 Size: 179 40 102 214 Fragments arranged by size: 468 214 180 179 102 77 61 51 45 40 29 15 BspEI T'CCGG_A Cuts at: 0 722 857 1461 Size: 722 135 604 Fragments arranged by size: 722 604 135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1015 1259 1313 1375 1461 Size: 1015 244 54 62 86 Fragments arranged by size: 1015 244 86 62 54 BsrBI CCG'CTC Cuts at: 0 285 660 1314 1461 Size: 285 375 654 147 Fragments arranged by size: 654 375 285 147 BsrDI GCAATG_nn' Cuts at: 0 705 1461 Size: 705 756 BsrFI r'CCGG_y Cuts at: 0 35 167 1222 1461 Size: 35 132 1055 239 Fragments arranged by size: 1055 239 132 35 BssSI C'ACGA_G Cuts at: 0 1375 1461 Size: 1375 86 BstAPI GCAn_nnn'nTGC Cuts at: 0 113 1461 Size: 113 1348 BstXI CCAn_nnnn'nTGG Cuts at: 0 57 1461 Size: 57 1404 Bsu36I CC'TnA_GG Cuts at: 0 232 1461 Size: 232 1229 BtgI C'CryG_G Cuts at: 0 603 737 1023 1217 1400 1461 Size: 603 134 286 194 183 61 Fragments arranged by size: 603 286 194 183 134 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 444 1461 Size: 444 1017 BtsI GCAGTG_nn' Cuts at: 0 94 902 955 1461 Size: 94 808 53 506 Fragments arranged by size: 808 506 94 53 DraIII CAC_nnn'GTG Cuts at: 0 555 1149 1461 Size: 555 594 312 Fragments arranged by size: 594 555 312 EaeI y'GGCC_r Cuts at: 0 93 212 1016 1038 1220 1224 1309 1461 Size: 93 119 804 22 182 4 85 152 Fragments arranged by size: 804 182 152 119 93 85 22 4 EagI C'GGCC_G Cuts at: 0 1038 1309 1461 Size: 1038 271 152 Fragments arranged by size: 1038 271 152 EarI CTCTTCn'nnn_ Cuts at: 0 859 1461 Size: 859 602 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 514 1461 Size: 514 947 EcoICRI GAG'CTC Cuts at: 0 315 405 456 924 1461 Size: 315 90 51 468 537 Fragments arranged by size: 537 468 315 90 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 49 79 448 495 601 655 729 Size: 25 24 30 369 47 106 54 74 Cuts at: 729 940 1186 1461 Size: 211 246 275 Fragments arranged by size: 369 275 246 211 106 74 54 47 30 25 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 817 1168 1461 Size: 817 351 293 Fragments arranged by size: 817 351 293 EcoO109I rG'GnC_Cy Cuts at: 0 246 1133 1461 Size: 246 887 328 Fragments arranged by size: 887 328 246 FseI GG_CCGG'CC Cuts at: 0 1226 1461 Size: 1226 235 HaeII r_GCGC'y Cuts at: 0 993 1086 1461 Size: 993 93 375 Fragments arranged by size: 993 375 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 579 611 1461 Size: 579 32 850 Fragments arranged by size: 850 579 32 KasI G'GCGC_C Cuts at: 0 1082 1461 Size: 1082 379 KpnI G_GTAC'C Cuts at: 0 1210 1461 Size: 1210 251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 482 872 1461 Size: 482 390 589 Fragments arranged by size: 589 482 390 MscI TGG'CCA Cuts at: 0 95 214 1018 1461 Size: 95 119 804 443 Fragments arranged by size: 804 443 119 95 MslI CAynn'nnrTG Cuts at: 0 140 602 943 1461 Size: 140 462 341 518 Fragments arranged by size: 518 462 341 140 MspA1I CmG'CkG Cuts at: 0 515 575 633 969 1025 1421 1461 Size: 515 60 58 336 56 396 40 Fragments arranged by size: 515 396 336 60 58 56 40 NaeI GCC'GGC Cuts at: 0 169 1224 1461 Size: 169 1055 237 Fragments arranged by size: 1055 237 169 NarI GG'CG_CC Cuts at: 0 1083 1461 Size: 1083 378 NcoI C'CATG_G Cuts at: 0 737 1400 1461 Size: 737 663 61 Fragments arranged by size: 737 663 61 NgoMIV G'CCGG_C Cuts at: 0 167 1222 1461 Size: 167 1055 239 Fragments arranged by size: 1055 239 167 NotI GC'GGCC_GC Cuts at: 0 1038 1309 1461 Size: 1038 271 152 Fragments arranged by size: 1038 271 152 NspI r_CATG'y Cuts at: 0 630 1461 Size: 630 831 PasI CC'CwG_GG Cuts at: 0 241 645 1461 Size: 241 404 816 Fragments arranged by size: 816 404 241 PflMI CCAn_nnn'nTGG Cuts at: 0 555 738 1461 Size: 555 183 723 Fragments arranged by size: 723 555 183 PfoI T'CCnGG_A Cuts at: 0 536 917 1358 1461 Size: 536 381 441 103 Fragments arranged by size: 536 441 381 103 PpuMI rG'GwC_Cy Cuts at: 0 246 1133 1461 Size: 246 887 328 Fragments arranged by size: 887 328 246 PstI C_TGCA'G Cuts at: 0 89 1461 Size: 89 1372 PvuII CAG'CTG Cuts at: 0 515 633 1461 Size: 515 118 828 Fragments arranged by size: 828 515 118 SacI G_AGCT'C Cuts at: 0 317 407 458 926 1461 Size: 317 90 51 468 535 Fragments arranged by size: 535 468 317 90 51 SacII CC_GC'GG Cuts at: 0 1026 1461 Size: 1026 435 SanDI GG'GwC_CC Cuts at: 0 246 1461 Size: 246 1215 SapI GCTCTTCn'nnn_ Cuts at: 0 859 1461 Size: 859 602 SexAI A'CCwGG_T Cuts at: 0 551 1461 Size: 551 910 SfcI C'TryA_G Cuts at: 0 85 384 999 1461 Size: 85 299 615 462 Fragments arranged by size: 615 462 299 85 SfoI GGC'GCC Cuts at: 0 1084 1461 Size: 1084 377 SmaI CCC'GGG Cuts at: 0 655 1204 1461 Size: 655 549 257 Fragments arranged by size: 655 549 257 SmlI C'TyrA_G Cuts at: 0 886 1461 Size: 886 575 StuI AGG'CCT Cuts at: 0 812 1461 Size: 812 649 StyI C'CwwG_G Cuts at: 0 257 737 929 1400 1461 Size: 257 480 192 471 61 Fragments arranged by size: 480 471 257 192 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 507 926 1461 Size: 507 419 535 Fragments arranged by size: 535 507 419 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 207 1461 Size: 207 1254 XmaI C'CCGG_G Cuts at: 0 653 1202 1461 Size: 653 549 259 Fragments arranged by size: 653 549 259 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PfoI PpuMI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII XcmI XmaI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaI BsaAI BsaBI BsaXI BsiWI BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI