(Circular) (Six-base) MAPSORT of: pFN21AE4114.seq Check: 8785 from: 1 to: 6166 pFN21AE4114 6166 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2005 3020 3380 2005 Size: 1015 360 4791 Fragments arranged by size: 4791 1015 360 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3976 278 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2388 3509 1937 Size: 451 1121 4594 Fragments arranged by size: 4594 1121 451 Acc65I G'GTAC_C Cuts at: 3211 3487 3211 Size: 276 5890 AclI AA'CG_TT Cuts at: 1563 4293 4666 1563 Size: 2730 373 3063 Fragments arranged by size: 3063 2730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2453 2500 3191 4229 5613 5881 1632 Size: 398 423 47 691 1038 1384 268 1917 Fragments arranged by size: 1917 1384 1038 691 423 398 268 47 AfeI AGC'GCT Cuts at: 2996 2996 Size: 6166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5969 AflIII A'CryG_T Cuts at: 1203 5065 6042 1203 Size: 3862 977 1327 Fragments arranged by size: 3862 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 4895 4895 Size: 6166 AleI CACnn'nnGTG Cuts at: 1166 2607 2948 1166 Size: 1441 341 4384 Fragments arranged by size: 4384 1441 341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2216 3416 1184 Size: 1032 1200 3934 Fragments arranged by size: 3934 1200 1032 AlwNI CAG_nnn'CTG Cuts at: 2548 3012 3909 5481 2548 Size: 464 897 1572 3233 Fragments arranged by size: 3233 1572 897 464 ApaLI G'TGCA_C Cuts at: 2273 4223 5379 2273 Size: 1950 1156 3060 Fragments arranged by size: 3060 1950 1156 ApoI r'AATT_y Cuts at: 1493 3475 3701 3853 5962 1493 Size: 1982 226 152 2109 1697 Fragments arranged by size: 2109 1982 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4720 160 Size: 872 3688 1606 Fragments arranged by size: 3688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 2658 3143 3207 3446 3491 1870 Size: 73 715 485 64 239 45 4545 Fragments arranged by size: 4545 715 485 239 73 64 45 BamHI G'GATC_C Cuts at: 3496 3863 3496 Size: 367 5799 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3087 3211 3487 4942 618 Size: 325 217 627 1300 124 276 1455 1842 Fragments arranged by size: 1842 1455 1300 627 325 276 217 124 BanII G_rGCy'C Cuts at: 1391 2082 2262 2322 2351 2412 2463 2931 3252 Size: 691 180 60 29 61 51 468 321 Cuts at: 3252 3485 1391 Size: 233 4072 Fragments arranged by size: 4072 691 468 321 233 180 61 60 51 29 BbeI G_GCGC'C Cuts at: 3091 3091 Size: 6166 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2062 2506 928 Size: 971 163 444 4588 Fragments arranged by size: 4588 971 444 163 BbvCI CC'TCA_GC Cuts at: 3440 3440 Size: 6166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3179 3213 4357 4391 1903 Size: 34 1242 34 1144 34 3678 Fragments arranged by size: 3678 1242 1144 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4058 5268 4058 Size: 1210 4956 BclI T'GATC_A Cuts at: 1548 1548 Size: 6166 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3123 3427 4777 Size: 107 122 71 631 605 1451 304 1350 Cuts at: 4777 136 Size: 1525 Fragments arranged by size: 1525 1451 1350 631 605 304 122 107 71 BglII A'GATC_T Cuts at: 6161 6161 Size: 6166 BlpI GC'TnA_GC Cuts at: 2291 3583 2291 Size: 1292 4874 Bme1580I G_kGCm'C Cuts at: 1790 2277 3110 4227 5383 1790 Size: 487 833 1117 1156 2573 Fragments arranged by size: 2573 1156 1117 833 487 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3064 3897 4855 Size: 416 53 83 399 24 1647 833 958 Cuts at: 4855 442 Size: 1753 Fragments arranged by size: 1753 1647 958 833 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2084 2606 2660 2734 2945 4826 1239 Size: 815 30 522 54 74 211 1881 2579 Fragments arranged by size: 2579 1881 815 522 211 74 54 30 Bpu10I CC'TnA_GC Cuts at: 2991 3440 2991 Size: 449 5717 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2876 4291 5156 5454 2876 Size: 1415 865 298 3588 Fragments arranged by size: 3588 1415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4829 882 Size: 617 3330 2219 Fragments arranged by size: 3330 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3957 493 Size: 711 2753 2702 Fragments arranged by size: 2753 2702 711 BsaBI GATnn'nnATC Cuts at: 6160 6160 Size: 6166 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3088 3973 4355 4990 Size: 53 83 186 936 1555 885 382 635 Cuts at: 4990 275 Size: 1451 Fragments arranged by size: 1555 1451 936 885 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2280 2484 2727 2862 4599 4997 5271 5418 5829 Size: 204 243 135 1737 398 274 147 411 Cuts at: 5829 2280 Size: 2617 Fragments arranged by size: 2617 1737 411 398 274 243 204 147 135 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5941 Fragments arranged by size: 5941 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2582 1186 Size: 1234 162 4770 Fragments arranged by size: 4770 1234 162 BseYI C'CCAG_C Cuts at: 2344 2520 2970 3422 5369 5865 2344 Size: 176 450 452 1947 496 2645 Fragments arranged by size: 2645 1947 496 452 450 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 2972 3242 1163 Size: 573 798 438 270 4087 Fragments arranged by size: 4087 798 573 438 270 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3298 3317 3616 4377 5405 Size: 275 186 1045 252 19 299 761 1028 Cuts at: 5405 1540 Size: 2301 Fragments arranged by size: 2301 1045 1028 761 299 275 252 186 19 BsiHKAI G_wGCw'C Cuts at: 2277 2322 2412 2463 2931 3150 4227 4312 5383 Size: 45 90 51 468 219 1077 85 1071 Cuts at: 5383 2277 Size: 3060 Fragments arranged by size: 3060 1077 1071 468 219 90 85 51 45 BsmI GAATG_Cn' Cuts at: 2789 3677 3770 2789 Size: 888 93 5185 Fragments arranged by size: 5185 888 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2215 2383 2215 Size: 168 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2262 2277 2322 2351 2412 2463 Size: 399 292 180 15 45 29 61 51 Cuts at: 2463 2931 3110 3150 3252 3485 4227 4312 5383 Size: 468 179 40 102 233 742 85 1071 Cuts at: 5383 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 468 399 292 233 180 179 102 85 61 51 45 40 29 15 BspEI T'CCGG_A Cuts at: 2727 2862 5829 2727 Size: 135 2967 3064 Fragments arranged by size: 3064 2967 135 BspHI T'CATG_A Cuts at: 4055 5785 4055 Size: 1730 4436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2005 3020 3264 3318 3380 3521 844 Size: 1161 1015 244 54 62 141 3489 Fragments arranged by size: 3489 1161 1015 244 141 62 54 BsrBI CCG'CTC Cuts at: 1394 1823 2290 2665 3319 4053 1394 Size: 429 467 375 654 734 3507 Fragments arranged by size: 3507 734 654 467 429 375 BsrDI GCAATG_nn' Cuts at: 65 1248 2710 4661 4835 65 Size: 1183 1462 1951 174 1396 Fragments arranged by size: 1951 1462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2172 3227 4810 4997 1700 Size: 110 230 132 1055 1583 187 2869 Fragments arranged by size: 2869 1583 1055 230 187 132 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6166 BssSI C'ACGA_G Cuts at: 3380 4226 5238 3380 Size: 846 1012 4308 Fragments arranged by size: 4308 1012 846 BstAPI GCAn_nnn'nTGC Cuts at: 794 2118 794 Size: 1324 4842 BstBI TT'CG_AA Cuts at: 4970 4970 Size: 6166 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6166 BstXI CCAn_nnnn'nTGG Cuts at: 2062 3463 2062 Size: 1401 4765 BstYI r'GATC_y Cuts at: 1972 3496 3863 4248 4265 5717 5803 5815 6161 Size: 1524 367 385 17 1452 86 12 346 Cuts at: 6161 1972 Size: 1977 Fragments arranged by size: 1977 1524 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2237 2237 Size: 6166 BtgI C'CryG_G Cuts at: 513 1065 2004 2608 2742 3028 3222 3405 513 Size: 552 939 604 134 286 194 183 3274 Fragments arranged by size: 3274 939 604 552 286 194 183 134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2449 487 Size: 683 56 1223 4204 Fragments arranged by size: 4204 1223 683 56 BtsI GCAGTG_nn' Cuts at: 2099 2907 2960 3682 4476 4496 2099 Size: 808 53 722 794 20 3769 Fragments arranged by size: 3769 808 794 722 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 3470 3470 Size: 6166 DraIII CAC_nnn'GTG Cuts at: 2560 3154 2560 Size: 594 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5173 809 Size: 507 3857 1802 Fragments arranged by size: 3857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2217 3021 Size: 54 1425 50 275 18 268 119 804 Cuts at: 3021 3043 3225 3229 3314 3613 4502 6131 8 Size: 22 182 4 85 299 889 1629 43 Fragments arranged by size: 1629 1425 889 804 299 275 268 182 119 85 54 50 43 22 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3043 3314 3613 1537 Size: 275 1231 271 299 4090 Fragments arranged by size: 4090 1231 299 275 271 EarI CTCTTCn'nnn_ Cuts at: 1353 2864 4096 1353 Size: 1511 1232 3423 Fragments arranged by size: 3423 1511 1232 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2519 4753 5127 5273 387 Size: 1064 138 930 2234 374 146 1280 Fragments arranged by size: 2234 1280 1064 930 374 146 138 EcoICRI GAG'CTC Cuts at: 2320 2410 2461 2929 2320 Size: 90 51 468 5557 Fragments arranged by size: 5557 468 90 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2054 2084 2453 2500 2606 2660 Size: 393 398 24 30 369 47 106 54 Cuts at: 2660 2734 2945 3191 4229 4826 5613 5881 1239 Size: 74 211 246 1038 597 787 268 1524 Fragments arranged by size: 1524 1038 787 597 398 393 369 268 246 211 106 74 54 47 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 2822 3173 2822 Size: 351 5815 EcoO109I rG'GnC_Cy Cuts at: 2251 3138 2251 Size: 887 5279 EcoRI G'AATT_C Cuts at: 3475 3853 3475 Size: 378 5788 FseI GG_CCGG'CC Cuts at: 3231 3231 Size: 6166 FspI TGC'GCA Cuts at: 4672 4672 Size: 6166 HaeII r_GCGC'y Cuts at: 1678 2998 3091 5313 1678 Size: 1320 93 2222 2531 Fragments arranged by size: 2531 2222 1320 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2584 2616 4807 4839 4881 Size: 32 135 32 1012 32 2191 32 42 Cuts at: 4881 4913 1373 Size: 32 2626 Fragments arranged by size: 2626 2191 1012 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3510 3756 669 Size: 988 281 1572 246 3079 Fragments arranged by size: 3079 1572 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6166 HpaI GTT'AAC Cuts at: 3756 3756 Size: 6166 KasI G'GCGC_C Cuts at: 3087 3087 Size: 6166 KpnI G_GTAC'C Cuts at: 3215 3491 3215 Size: 276 5890 MfeI C'AATT_G Cuts at: 3765 3765 Size: 6166 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 2877 5280 5464 1305 Size: 1182 390 2403 184 2007 Fragments arranged by size: 2403 2007 1182 390 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 2219 3023 10 Size: 54 1425 343 268 119 804 3153 Fragments arranged by size: 3153 1425 804 343 268 119 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2607 2948 4124 4483 4642 518 Size: 648 979 462 341 1176 359 159 2042 Fragments arranged by size: 2042 1176 979 648 462 359 341 159 MspA1I CmG'CkG Cuts at: 1606 2520 2580 2638 2974 3030 3426 3582 4259 Size: 914 60 58 336 56 396 156 677 Cuts at: 4259 5407 5652 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 914 677 396 336 245 156 60 58 56 NaeI GCC'GGC Cuts at: 2174 3229 2174 Size: 1055 5111 NarI GG'CG_CC Cuts at: 3088 3088 Size: 6166 NcoI C'CATG_G Cuts at: 513 1065 2004 2742 3405 513 Size: 552 939 738 663 3274 Fragments arranged by size: 3274 939 738 663 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6166 NgoMIV G'CCGG_C Cuts at: 2172 3227 2172 Size: 1055 5111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6166 NotI GC'GGCC_GC Cuts at: 3043 3314 3613 3043 Size: 271 299 5596 Fragments arranged by size: 5596 299 271 NspI r_CATG'y Cuts at: 1132 1229 2635 3524 5069 1132 Size: 97 1406 889 1545 2229 Fragments arranged by size: 2229 1545 1406 889 97 PasI CC'CwG_GG Cuts at: 1348 2246 2650 1348 Size: 898 404 4864 Fragments arranged by size: 4864 898 404 PciI A'CATG_T Cuts at: 5065 5065 Size: 6166 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2560 2743 1108 Size: 902 550 183 4531 Fragments arranged by size: 4531 902 550 183 PfoI T'CCnGG_A Cuts at: 2541 2922 3363 2541 Size: 381 441 5344 Fragments arranged by size: 5344 441 381 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2251 3138 2251 Size: 887 5279 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6166 PsiI TTA'TAA Cuts at: 3736 3736 Size: 6166 PstI C_TGCA'G Cuts at: 830 2094 3518 830 Size: 1264 1424 3478 Fragments arranged by size: 3478 1424 1264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2520 2638 2520 Size: 118 6048 SacI G_AGCT'C Cuts at: 2322 2412 2463 2931 2322 Size: 90 51 468 5557 Fragments arranged by size: 5557 468 90 51 SacII CC_GC'GG Cuts at: 3031 3031 Size: 6166 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SanDI GG'GwC_CC Cuts at: 2251 2251 Size: 6166 SapI GCTCTTCn'nnn_ Cuts at: 2864 2864 Size: 6166 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 4414 6126 4414 Size: 1712 4454 SexAI A'CCwGG_T Cuts at: 2556 2556 Size: 6166 SfcI C'TryA_G Cuts at: 826 1045 2090 2389 3004 3514 4649 5330 5521 Size: 219 1045 299 615 510 1135 681 191 Cuts at: 5521 826 Size: 1471 Fragments arranged by size: 1471 1135 1045 681 615 510 299 219 191 SfoI GGC'GCC Cuts at: 3089 3089 Size: 6166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 2660 3209 3493 1872 Size: 788 549 284 4545 Fragments arranged by size: 4545 788 549 284 SmlI C'TyrA_G Cuts at: 820 1017 1943 2891 4270 5171 5433 820 Size: 197 926 948 1379 901 262 1553 Fragments arranged by size: 1553 1379 948 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3957 493 Size: 3464 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 3524 1132 Size: 2392 3774 SspI AAT'ATT Cuts at: 5 52 4090 5 Size: 47 4038 2081 Fragments arranged by size: 4038 2081 47 StuI AGG'CCT Cuts at: 1517 2817 1517 Size: 1300 4866 StyI C'CwwG_G Cuts at: 513 1065 2004 2262 2742 2934 3405 3605 513 Size: 552 939 258 480 192 471 200 3074 Fragments arranged by size: 3074 939 552 480 471 258 200 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2512 2931 4193 4210 4363 4548 4993 Size: 1255 932 419 1262 17 153 185 445 Cuts at: 4993 325 Size: 1498 Fragments arranged by size: 1498 1262 1255 932 445 419 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4412 6124 96 Size: 274 80 33 51 1443 2435 1712 138 Fragments arranged by size: 2435 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6126 6158 6126 Size: 32 6134 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6166 XbaI T'CTAG_A Cuts at: 3502 3502 Size: 6166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 2212 Size: 6166 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6166 XmaI C'CCGG_G Cuts at: 1870 2658 3207 3491 1870 Size: 788 549 284 4545 Fragments arranged by size: 4545 788 549 284 XmnI GAAnn'nnTTC Cuts at: 4295 4295 Size: 6166 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3974 276 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SfiI SrfI SwaI