FBXO27 ORF clone pFN21AE3995

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3995

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Clone info.

Species

human

Clone Name

pFN21AE3995 Sequence (with vector) Restriction Map

Product ID

FHC26619

ORF Sequence

DNA sequence (849 bp)
Protein sequence (283 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3995

Symbol

FBXO27

Alias : FBG5, Fbx27

Description

F-box protein 27

Original Clone ID

cp93995

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 849 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 283 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12527.1 7.3e-124 100.0 FBXO27 CCDS12526.1 1.6e-68 57.7 FBXO17 CCDS133.1 1.7e-37 40.8 FBXO6 CCDS130.1 1e-36 41.8 FBXO2 CCDS132.1 6.7e-33 39.4 FBXO44 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001810 26 71 PF00646 F-box domain IPR007397 106 279 PF04300 F-box associated (FBA) domain HMMSmart IPR001810 29 70 SM00256 F-box domain ProfileScan IPR001810 23 70 PS50181 F-box domain IPR007397 104 280 PS51114 F-box associated (FBA) domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_849142 283 609099 100.0 100.0 XP_016881779 249 609099 100.0 83.4 NP_079183 278 609094 57.7 100.0 NP_680474 287 609094 57.7 100.0 XP_005263505 293 605647 40.8 94.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3995 Download

>KIEE3995  849 bp
ATGGGCGCCTCGGTCTCCAGGGGCCGGGCCGCCCGGGTCCCCGCGCCGGAGCCGGAACCC
GAAGAGGCGCTGGACCTGAGCCAACTACCCCCAGAGCTGCTTCTGGTGGTGCTGAGCCAC
GTCCCCCCGCGCACGCTGCTCGGGCGCTGCCGCCAAGTGTGCCGGGGCTGGCGAGCCCTG
GTGGACGGCCAGGCCCTGTGGCTGCTGATCCTGGCCCGCGACCACGGCGCCACCGGCCGC
GCGCTGCTGCACCTCGCCCGCAGCTGCCAGTCTCCCGCCCGTAACGCCAGGCCTTGCCCC
CTGGGCCGCTTCTGCGCGCGCAGACCCATCGGACGCAACCTTATTCGCAACCCCTGCGGC
CAAGAAGGCCTCCGAAAGTGGATGGTGCAACACGGTGGGGACGGCTGGGTGGTGGAGGAA
AACAGGACAACCGTGCCTGGGGCCCCTTCTCAGACGTGCTTCGTGACTTCATTCAGCTGG
TGTTGCAAGAAGCAGGTCTTGGACCTAGAGGAGGAGGGTCTGTGGCCAGAACTGCTGGAT
AGTGGCAGGATTGAGATTTGTGTCTCTGACTGGTGGGGAGCCCGACACGACAGCGGCTGT
ATGTACAGACTCCTCGTCCAACTTCTAGACGCCAACCAGACTGTTCTAGATAAATTCTCT
GCTGTGCCTGATCCCATCCCGCAGTGGAACAACAATGCCTGCCTTCACGTCACCCACGTG
TTCTCCAACATCAAGATGGGCGTCCGCTTTGTGTCTTTCGAACACCGGGGCCAGGACACA
CAGTTCTGGGCTGGCCACTATGGAGCCCGTGTGACCAACTCCAGTGTGATCGTGCGAGTC
CGTCTGTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE3995 Download

>KIEE3995  283 aa
MGASVSRGRAARVPAPEPEPEEALDLSQLPPELLLVVLSHVPPRTLLGRCRQVCRGWRAL
VDGQALWLLILARDHGATGRALLHLARSCQSPARNARPCPLGRFCARRPIGRNLIRNPCG
QEGLRKWMVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLD
SGRIEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLHVTHV
FSNIKMGVRFVSFEHRGQDTQFWAGHYGARVTNSSVIVRVRLS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3995 Download

>pFN21AE3995  5554 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCGCCTCGGTCTCCAGGGGCCGGGCCGCCCG
GGTCCCCGCGCCGGAGCCGGAACCCGAAGAGGCGCTGGACCTGAGCCAACTACCCCCAGA
GCTGCTTCTGGTGGTGCTGAGCCACGTCCCCCCGCGCACGCTGCTCGGGCGCTGCCGCCA
AGTGTGCCGGGGCTGGCGAGCCCTGGTGGACGGCCAGGCCCTGTGGCTGCTGATCCTGGC
CCGCGACCACGGCGCCACCGGCCGCGCGCTGCTGCACCTCGCCCGCAGCTGCCAGTCTCC
CGCCCGTAACGCCAGGCCTTGCCCCCTGGGCCGCTTCTGCGCGCGCAGACCCATCGGACG
CAACCTTATTCGCAACCCCTGCGGCCAAGAAGGCCTCCGAAAGTGGATGGTGCAACACGG
TGGGGACGGCTGGGTGGTGGAGGAAAACAGGACAACCGTGCCTGGGGCCCCTTCTCAGAC
GTGCTTCGTGACTTCATTCAGCTGGTGTTGCAAGAAGCAGGTCTTGGACCTAGAGGAGGA
GGGTCTGTGGCCAGAACTGCTGGATAGTGGCAGGATTGAGATTTGTGTCTCTGACTGGTG
GGGAGCCCGACACGACAGCGGCTGTATGTACAGACTCCTCGTCCAACTTCTAGACGCCAA
CCAGACTGTTCTAGATAAATTCTCTGCTGTGCCTGATCCCATCCCGCAGTGGAACAACAA
TGCCTGCCTTCACGTCACCCACGTGTTCTCCAACATCAAGATGGGCGTCCGCTTTGTGTC
TTTCGAACACCGGGGCCAGGACACACAGTTCTGGGCTGGCCACTATGGAGCCCGTGTGAC
CAACTCCAGTGTGATCGTGCGAGTCCGTCTGTCCGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT