(Linear) (Six-base) MAPSORT of: KIEE3995.seq Check: 150 from: 1 to: 849 KIEE3995.orf With 182 enzymes: SgfI * March 22, 2012 17:06 .. AflIII A'CryG_T Cuts at: 0 716 849 Size: 716 133 AhdI GACnn_n'nnGTC Cuts at: 0 613 849 Size: 613 236 AleI CACnn'nnGTG Cuts at: 0 715 849 Size: 715 134 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 766 798 849 Size: 766 32 51 Fragments arranged by size: 766 51 32 AlwNI CAG_nnn'CTG Cuts at: 0 195 596 849 Size: 195 401 253 Fragments arranged by size: 401 253 195 ApaI G_GGCC'C Cuts at: 0 444 849 Size: 444 405 ApoI r'AATT_y Cuts at: 0 652 849 Size: 652 197 AvaI C'yCGr_G Cuts at: 0 32 139 849 Size: 32 107 710 Fragments arranged by size: 710 107 32 BanI G'GyrC_C Cuts at: 0 4 226 849 Size: 4 222 623 Fragments arranged by size: 623 222 4 BanII G_rGCy'C Cuts at: 0 177 444 582 807 849 Size: 177 267 138 225 42 Fragments arranged by size: 267 225 177 138 42 BbeI G_GCGC'C Cuts at: 0 8 230 849 Size: 8 222 619 Fragments arranged by size: 619 222 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 120 154 309 343 849 Size: 120 34 155 34 506 Fragments arranged by size: 506 155 120 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 302 365 849 Size: 302 63 484 Fragments arranged by size: 484 302 63 BlpI GC'TnA_GC Cuts at: 0 112 849 Size: 112 737 Bme1580I G_kGCm'C Cuts at: 0 444 849 Size: 444 405 BmgBI CAC'GTC Cuts at: 0 120 455 708 849 Size: 120 335 253 141 Fragments arranged by size: 335 253 141 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 804 849 Size: 804 45 Bpu10I CC'TnA_GC Cuts at: 0 76 849 Size: 76 773 BsaI GGTCTCn'nnnn_ Cuts at: 0 18 849 Size: 18 831 BsaAI yAC'GTr Cuts at: 0 717 849 Size: 717 132 BsaHI Gr'CG_yC Cuts at: 0 5 227 629 740 849 Size: 5 222 402 111 109 Fragments arranged by size: 402 222 111 109 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 505 535 706 736 794 824 849 Size: 505 30 171 30 58 30 25 Fragments arranged by size: 505 171 58 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 523 526 601 849 Size: 523 3 75 248 Fragments arranged by size: 523 248 75 3 BseYI C'CCAG_C Cuts at: 0 404 849 Size: 404 445 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 849 Size: 232 617 BsiEI CG_ry'CG Cuts at: 0 237 849 Size: 237 612 Bsp1286I G_dGCh'C Cuts at: 0 177 444 582 807 849 Size: 177 267 138 225 42 Fragments arranged by size: 267 225 177 138 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 483 849 Size: 483 366 BsrFI r'CCGG_y Cuts at: 0 232 849 Size: 232 617 BsrGI T'GTAC_A Cuts at: 0 602 849 Size: 602 247 BssHII G'CGCG_C Cuts at: 0 239 314 316 849 Size: 239 75 2 533 Fragments arranged by size: 533 239 75 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 353 849 Size: 353 496 BstBI TT'CG_AA Cuts at: 0 758 849 Size: 758 91 BtgI C'CryG_G Cuts at: 0 222 849 Size: 222 627 BtsI GCAGTG_nn' Cuts at: 0 688 849 Size: 688 161 EaeI y'GGCC_r Cuts at: 0 186 234 357 523 792 849 Size: 186 48 123 166 269 57 Fragments arranged by size: 269 186 166 123 57 48 EagI C'GGCC_G Cuts at: 0 234 849 Size: 234 615 EarI CTCTTCn'nnn_ Cuts at: 0 56 849 Size: 56 793 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 804 849 Size: 804 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 508 849 Size: 508 341 EcoO109I rG'GnC_Cy Cuts at: 0 36 192 440 441 849 Size: 36 156 248 1 408 Fragments arranged by size: 408 248 156 36 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 513 849 Size: 513 336 HaeII r_GCGC'y Cuts at: 0 8 70 147 230 849 Size: 8 62 77 83 619 Fragments arranged by size: 619 83 77 62 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 599 631 640 672 849 Size: 599 32 9 32 177 Fragments arranged by size: 599 177 32 32 9 KasI G'GCGC_C Cuts at: 0 4 226 849 Size: 4 222 623 Fragments arranged by size: 623 222 4 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 642 749 849 Size: 642 107 100 Fragments arranged by size: 642 107 100 MscI TGG'CCA Cuts at: 0 525 794 849 Size: 525 269 55 Fragments arranged by size: 525 269 55 MslI CAynn'nnrTG Cuts at: 0 715 733 849 Size: 715 18 116 Fragments arranged by size: 715 116 18 MspA1I CmG'CkG Cuts at: 0 263 476 593 849 Size: 263 213 117 256 Fragments arranged by size: 263 256 213 117 NarI GG'CG_CC Cuts at: 0 5 227 849 Size: 5 222 622 Fragments arranged by size: 622 222 5 PasI CC'CwG_GG Cuts at: 0 300 849 Size: 300 549 PmlI CAC'GTG Cuts at: 0 717 849 Size: 717 132 PpuMI rG'GwC_Cy Cuts at: 0 36 849 Size: 36 813 PspOMI G'GGCC_C Cuts at: 0 440 849 Size: 440 409 PvuII CAG'CTG Cuts at: 0 263 476 849 Size: 263 213 373 Fragments arranged by size: 373 263 213 SanDI GG'GwC_CC Cuts at: 0 36 849 Size: 36 813 SfiI GGCCn_nnn'nGGCC Cuts at: 0 365 849 Size: 365 484 SfoI GGC'GCC Cuts at: 0 6 228 849 Size: 6 222 621 Fragments arranged by size: 621 222 6 SmaI CCC'GGG Cuts at: 0 34 849 Size: 34 815 StuI AGG'CCT Cuts at: 0 291 368 849 Size: 291 77 481 Fragments arranged by size: 481 291 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 396 727 849 Size: 396 331 122 Fragments arranged by size: 396 331 122 TatI w'GTAC_w Cuts at: 0 602 849 Size: 602 247 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 505 537 706 738 794 826 849 Size: 505 32 169 32 56 32 23 Fragments arranged by size: 505 169 56 32 32 32 23 XbaI T'CTAG_A Cuts at: 0 624 645 849 Size: 624 21 204 Fragments arranged by size: 624 204 21 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 732 849 Size: 732 117 XmaI C'CCGG_G Cuts at: 0 32 849 Size: 32 817 Enzymes that do cut: AflIII AhdI AleI AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrFI BsrGI BssHII BstAPI BstBI BtgI BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI PasI PmlI PpuMI PspOMI PvuII SanDI SfiI SfoI SmaI StuI TaqII TatI TstI XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaBI BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I XhoI XmnI ZraI