################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BamHI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssSI Bst1107I BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TfiI Tru9I Tth111I Van91I XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 652 AAATTC [GA]^AATT[CT] AfaI 603 GTAC GT^AC AflIII 716 ACGTGT A^C[GA][CT]GT AhdI 608 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 440 GGGCCC GGGCC^C ApoI 652 AAATTC [GA]^AATT[CT] AspEI 608 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 715 CACGTG CAC^GTG BlpI 111 GCTGAGC GC^T[ACGT]AGC Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC BsaAI 715 CACGTG [CT]AC^GT[GA] BsaI 12 GGTCTC GGTCTC(1/5) BsgI 247 CTGCAC GTGCAG(16/14) BsiEI 234 CGGCCG CG[GA][CT]^CG Bsp1407I 602 TGTACA T^GTACA BspMI 492 GCAGGT ACCTGC(4/8) BsrFI 232 ACCGGC [GA]^CCGG[CT] BsrGI 602 TGTACA T^GTACA BstAPI 347 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 757 TTCGAA TT^CGAA CelII 111 GCTGAGC GC^T[ACGT]AGC Cfr10I 232 ACCGGC [GA]^CCGG[CT] DsaI 222 CCACGG C^C[GA][CT]GG EagI 234 CGGCCG C^GGCCG Eam1105I 608 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 61 GAAGAG CTCTTC(1/4) EclXI 234 CGGCCG C^GGCCG Eco52I 234 CGGCCG C^GGCCG EcoNI 504 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HphI 710 TCACC GGTGA(8/7) Ksp632I 61 GAAGAG CTCTTC(1/4) MboII 61 GAAGA GAAGA(8/7) NspV 757 TTCGAA TT^CGAA PmaCI 715 CACGTG CAC^GTG PmlI 715 CACGTG CAC^GTG PpuMI 35 GGGTCCC [GA]G^G[AT]CC[CT] RsaI 603 GTAC GT^AC SfiI 358 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 757 TTCGAA TT^CGAA SmaI 32 CCCGGG CCC^GGG SspBI 602 TGTACA T^GTACA TaqI 758 TCGA T^CGA Tsp509I 653 AATT ^AATT TthHB8I 758 TCGA T^CGA XcmI 725 CCAACATCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 32 CCCGGG C^CCGGG XmaI 32 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 207 GATCC GGATC(4/5) 670 GATCC AlwNI 190 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 591 CAGCGGCTG AvaI 32 CCCGGG C^[CT]CG[GA]G 139 CTCGGG BalI 523 TGGCCA TGG^CCA 792 TGGCCA BanI 4 GGCGCC G^G[CT][GA]CC 226 GGCGCC BbeI 4 GGCGCC GGCGC^C 226 GGCGCC BcgI 131 GCACGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 320 GCAGACCCATCG BglI 296 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 359 GCCAAGAAGGC BpmI 15 CTCCAG CTGGAG(16/14) 819 CTCCAG BsoBI 32 CCCGGG C^[CT]CG[GA]G 139 CTCGGG BstF5I 675 CATCC GGATG(2/0) 380 GGATG FokI 675 CATCC GGATG(9/13) 380 GGATG HinfI 608 GACTC G^A[ACGT]TC 836 GAGTC KasI 4 GGCGCC G^GCGCC 226 GGCGCC MluNI 523 TGGCCA TGG^CCA 792 TGGCCA MscI 523 TGGCCA TGG^CCA 792 TGGCCA MslI 711 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 729 CATCAAGATG NarI 4 GGCGCC GG^CGCC 226 GGCGCC PleI 608 GACTC GAGTC(4/5) 836 GAGTC PvuII 261 CAGCTG CAG^CTG 474 CAGCTG SfoI 4 GGCGCC GGC^GCC 226 GGCGCC StuI 289 AGGCCT AGG^CCT 366 AGGCCT TspRI 682 CAGTG CA[GC]TG[ACGT][ACGT]^ 822 CAGTG XbaI 624 TCTAGA T^CTAGA 645 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BanII BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I