Restriction map for
pFN21AE3995
(Circular) (Six-base) MAPSORT of: pFN21AE3995.seq Check: 477 from: 1 to: 5554
pFN21AE3995 5554 bp
With 182 enzymes: SgfI *
March 28, 2012 11:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3617 5001 5269 1632
Size: 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 2721 4453 5430 1203
Size: 1518 1732 977 1327
Fragments arranged by size:
1732 1518 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 2618 4283 2618
Size: 1665 3889
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 4000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 5522
AlwNI CAG_nnn'CTG
Cuts at: 2200 2601 3297 4869 2200
Size: 401 696 1572 2885
Fragments arranged by size:
2885 1572 696 401
ApaI G_GGCC'C
Cuts at: 2449 2449
Size: 5554
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2657 2863 3089 3241 5350 1493
Size: 1164 206 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2144 2879 1870
Size: 73 94 107 735 4545
Fragments arranged by size:
4545 735 107 94 73
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2231 2875 4330 618
Size: 325 217 627 222 222 644 1455 1842
Fragments arranged by size:
1842 1455 644 627 325 222 222 217
BanII G_rGCy'C
Cuts at: 1391 2182 2449 2587 2812 2873 1391
Size: 791 267 138 225 61 4072
Fragments arranged by size:
4072 791 267 225 138 61
BbeI G_GCGC'C
Cuts at: 2013 2235 2013
Size: 222 5332
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2125 2159 2314 2348 3745 3779 1903
Size: 34 188 34 155 34 1397 34 3678
Fragments arranged by size:
3678 1397 188 155 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 2370 4165
Size: 107 122 71 631 605 635 63 1795
Cuts at: 4165 136
Size: 1525
Fragments arranged by size:
1795 1525 635 631 605 122 107 71
63
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2117 2971 2117
Size: 854 4700
Bme1580I G_kGCm'C
Cuts at: 1790 2449 3615 4771 1790
Size: 659 1166 1156 2573
Fragments arranged by size:
2573 1166 1156 659
BmgBI CAC'GTC
Cuts at: 1318 2125 2460 2713 1318
Size: 807 335 253 4159
Fragments arranged by size:
4159 807 335 253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 4243 442
Size: 416 53 83 399 24 1868 958 1753
Fragments arranged by size:
1868 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 2809 4214 1239
Size: 766 804 1405 2579
Fragments arranged by size:
2579 1405 804 766
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 5554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2023 4217 882
Size: 617 524 2194 2219
Fragments arranged by size:
2219 2194 617 524
BsaAI yAC'GTr
Cuts at: 493 1204 2722 3345 493
Size: 711 1518 623 2702
Fragments arranged by size:
2702 1518 711 623
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2232 2634 2745
Size: 53 83 186 936 477 222 402 111
Cuts at: 2745 3361 3743 4378 275
Size: 616 382 635 1451
Fragments arranged by size:
1451 936 635 616 477 402 382 222
186 111 83 53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2510 2540 2711 2741 2799
Size: 30 165 30 1107 30 171 30 58
Cuts at: 2799 2829 1178
Size: 30 3903
Fragments arranged by size:
3903 1107 171 165 58 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2528 2531 2606 1186
Size: 1342 3 75 4134
Fragments arranged by size:
4134 1342 75 3
BseYI C'CCAG_C
Cuts at: 2409 4757 5253 2409
Size: 2348 496 2710
Fragments arranged by size:
2710 2348 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 1163
Size: 573 501 4480
Fragments arranged by size:
4480 573 501
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2242 3004 3765 4793 1540
Size: 275 186 241 762 761 1028 2301
Fragments arranged by size:
2301 1028 762 761 275 241 186
BsiHKAI G_wGCw'C
Cuts at: 3615 3700 4771 3615
Size: 85 1071 4398
Fragments arranged by size:
4398 1071 85
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2182 2449 2587 2812 2873 3615 3700
Size: 399 392 267 138 225 61 742 85
Cuts at: 3700 4771 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 267 225 138
85 61
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 2909 844
Size: 1644 421 3489
Fragments arranged by size:
3489 1644 421
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2237 4198 4385 1700
Size: 110 427 1961 187 2869
Fragments arranged by size:
2869 1961 427 187 110
BsrGI T'GTAC_A
Cuts at: 96 2607 96
Size: 2511 3043
BssHII G'CGCG_C
Cuts at: 1925 2244 2319 2321 1925
Size: 319 75 2 5158
Fragments arranged by size:
5158 319 75 2
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2358 794
Size: 1564 3990
BstBI TT'CG_AA
Cuts at: 2763 4358 2763
Size: 1595 3959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2884 3251 3636 3653 5105 5191 5203 5549
Size: 912 367 385 17 1452 86 12 346
Cuts at: 5549 1972
Size: 1977
Fragments arranged by size:
1977 1452 912 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2227 513
Size: 552 939 223 3840
Fragments arranged by size:
3840 939 552 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 2693 3070 3864 3884 2693
Size: 377 794 20 4363
Fragments arranged by size:
4363 794 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 2239 2362
Size: 54 1425 50 275 18 361 48 123
Cuts at: 2362 2528 2797 3001 3890 5519 8
Size: 166 269 204 889 1629 43
Fragments arranged by size:
1629 1425 889 361 275 269 204 166
123 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2239 3001 1537
Size: 275 427 762 4090
Fragments arranged by size:
4090 762 427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 3484 1353
Size: 708 1423 3423
Fragments arranged by size:
3423 1423 708
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2809 3617 4214 5001 5269 1239
Size: 393 373 804 808 597 787 268 1524
Fragments arranged by size:
1524 808 804 787 597 393 373 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2513 2513
Size: 5554
EcoO109I rG'GnC_Cy
Cuts at: 2041 2197 2445 2446 2041
Size: 156 248 1 5149
Fragments arranged by size:
5149 248 156 1
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 5554
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 2013 2075 2152 2235 4701 1678
Size: 335 62 77 83 2466 2531
Fragments arranged by size:
2531 2466 335 83 77 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2604 2636 2645 2677 4195
Size: 32 135 32 1032 32 9 32 1518
Cuts at: 4195 4227 4269 4301 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1518 1032 135 42 32 32 32
32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KasI G'GCGC_C
Cuts at: 2009 2231 2009
Size: 222 5332
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2647 2754 4668 4852 1305
Size: 1342 107 1914 184 2007
Fragments arranged by size:
2007 1914 1342 184 107
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2530 2799 10
Size: 54 1425 343 698 269 2765
Fragments arranged by size:
2765 1425 698 343 269 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 2738 3512 3871 4030 518
Size: 648 1554 18 774 359 159 2042
Fragments arranged by size:
2042 1554 774 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2268 2481 2598 2970 3647 4795 5040 1606
Size: 662 213 117 372 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 662 372 245 213 117
NarI GG'CG_CC
Cuts at: 2010 2232 2010
Size: 222 5332
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4063
Fragments arranged by size:
4063 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2305 1348
Size: 957 4597
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PmlI CAC'GTG
Cuts at: 2722 2722
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2041
Size: 5554
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PspOMI G'GGCC_C
Cuts at: 2445 2445
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2906 830
Size: 2076 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2268 2481 2268
Size: 213 5341
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SanDI GG'GwC_CC
Cuts at: 2041 2041
Size: 5554
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2902 4037 4718 4909 826
Size: 219 1857 1135 681 191 1471
Fragments arranged by size:
1857 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2370 2370
Size: 5554
SfoI GGC'GCC
Cuts at: 2011 2233 2011
Size: 222 5332
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2039 2881 1872
Size: 167 842 4545
Fragments arranged by size:
4545 842 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 2296 2373 1517
Size: 779 77 4698
Fragments arranged by size:
4698 779 77
StyI C'CwwG_G
Cuts at: 513 1065 2004 2993 513
Size: 552 939 989 3074
Fragments arranged by size:
3074 989 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2005 2401 2732 3581 3598 3751 3936
Size: 1255 425 396 331 849 17 153 185
Cuts at: 3936 4381 325
Size: 445 1498
Fragments arranged by size:
1498 1255 849 445 425 396 331 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 3800 5512
Size: 274 80 33 51 1443 630 1193 1712
Cuts at: 5512 96
Size: 138
Fragments arranged by size:
1712 1443 1193 630 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 2711 2743 2799 2831 5514 5546 2510
Size: 32 169 32 56 32 2683 32 2518
Fragments arranged by size:
2683 2518 169 56 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2629 2650 2890 2629
Size: 21 240 5293
Fragments arranged by size:
5293 240 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 2737
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2037 2879 1870
Size: 167 842 4545
Fragments arranged by size:
4545 842 167
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacI SacII SapI SexAI SrfI
SwaI