MKRN2 ORF clone pFN21AE3912

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3912

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Clone info.

Species

human

Clone Name

pFN21AE3912 Sequence (with vector) Restriction Map

Product ID

FHC11056

ORF Sequence

DNA sequence (1248 bp)
Protein sequence (416 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3912

Symbol

MKRN2

Alias : HSPC070, RNF62

Description

makorin ring finger protein 2, transcript variant 1

Original Clone ID

cp93912

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1248 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 416 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33702.1 4.7e-140 99.8 MKRN2 CCDS63545.1 5.8e-122 99.7 MKRN2 CCDS5860.1 6.3e-46 46.8 MKRN1 CCDS10013.1 4.9e-39 41.9 MKRN3 CCDS47725.1 1.1e-24 46.8 MKRN1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000571 36 55 PF00642 Zinc finger IPR000571 169 191 PF00642 Zinc finger HMMSmart IPR000571 2 28 SM00356 Zinc finger IPR000571 32 57 SM00356 Zinc finger IPR000571 166 191 SM00356 Zinc finger IPR001841 238 291 SM00184 Zinc finger IPR000571 322 349 SM00356 Zinc finger ProfileScan IPR000571 2 29 PS50103 Zinc finger IPR000571 31 58 PS50103 Zinc finger IPR000571 165 192 PS50103 Zinc finger IPR001841 238 292 PS50089 Zinc finger IPR000571 321 350 PS50103 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_054879 416 608426 99.8 100.0 NP_001258636 373 608426 99.7 88.0 XP_011514299 418 607754 45.9 95.4 XP_011514300 418 607754 45.9 95.4 XP_011514298 439 607754 44.2 86.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3912 Download

>KIEE3912  1248 bp
ATGAGCACCAAGCAGATCACTTGCAGGTATTTTATGCATGGTGTGTGTCGGGAAGGAAGT
CAGTGCCTATTCTCACATGACTTGGCAAACAGCAAACCGTCCACCATCTGCAAGTACTAC
CAGAAGGGCTACTGTGCCTATGGAACTCGGTGCAGATATGACCACACGAGGCCCTCTGCT
GCAGCTGGAGGTGCTGTGGGCACCATGGCCCACAGTGTGCCCTCCCCAGCTTTCCACAGT
CCTCACCCTCCTTCCGAGGTCACTGCATCCATTGTGAAAACTAACTCACATGAACCCGGA
AAGCGTGAAAAGAGAACATTGGTTCTTAGAGACCGAAATCTCTCTGGCATGGCTGAAAGG
AAGACCCAGCCGAGCATGGTGAGTAATCCAGGCAGCTGCAGCGACCCCCAGCCCAGCCCC
GAGATGAAGCCGCATTCCTACCTGGATGCCATCAGGAGTGGCCTTGATGACGTGGAGGCC
AGCAGCTCCTACAGCAACGAGCAGCAGCTGTGCCCCTACGCAGCTGCTGGGGAGTGCCGG
TTTGGGGATGCCTGTGTCTACCTGCACGGGGAGGTGTGTGAAATCTGTAGGCTGCAAGTC
TTGCACCCATTCGACCCAGAGCAGAGGAAGGCTCACGAAAAGATCTGCATGTTGACGTTC
GAACACGAGATGGAAAAGGCCTTTGCCTTCCAGGCAAACCAGGACAAAGTGTGCAGTATC
TGCATGGAAGTGATCCTGGAGAAGGCCTCTGCTTCTGAGAGGAGATTTGGGATTCTCTCC
AATTGCAATCACACGTACTGTTTGTCCTGCATCCGGCAGTGGCGGTGTGCCAAACAGTTT
GAAAACCCAATCATTAAGTCTTGTCCAGAATGCCGTGTGATATCAGAGTTTGTAATTCCA
AGTGTGTATTGGGTGGAAGATCAGAATAAAAAGAACGAGTTGATTGAAGCTTTCAAACAG
GGGATGGGGAAAAAAGCCTGTAAATACTTTGAGCAAGGCAAGGGGACCTGCCCATTTGGA
AGCAAATGTCTTTATCGCCATGCTTACCCCGATGGGCGGCTAGCAGAGCCTGAGAAACCT
CGGAAACAGCTCAGTTCTCAAGGCACTGTGAGGTTCTTTAATTCAGTGCGGCTCTGGGAT
TTCATCGAGAACCGAGAAAGCCGGCATGTCCCCAACAATGAAGATGTCGACATGACAGAG
CTCGGGGACCTCTTCATGCACCTTTCTGGAGTGGAATCATCAGAACCC

ORF Protein

Cloned ORF protein sequence for pFN21AE3912 Download

>KIEE3912  416 aa
MSTKQITCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGTRCRYDHTRPSA
AAGGAVGTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKREKRTLVLRDRNLSGMAER
KTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEASSSYSNEQQLCPYAAAGECR
FGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICMLTFEHEMEKAFAFQANQDKVCSI
CMEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIP
SVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKP
RKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSEP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3912 Download

>pFN21AE3912  5953 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCACCAAGCAGATCACTTGCAGGTATTTTAT
GCATGGTGTGTGTCGGGAAGGAAGTCAGTGCCTATTCTCACATGACTTGGCAAACAGCAA
ACCGTCCACCATCTGCAAGTACTACCAGAAGGGCTACTGTGCCTATGGAACTCGGTGCAG
ATATGACCACACGAGGCCCTCTGCTGCAGCTGGAGGTGCTGTGGGCACCATGGCCCACAG
TGTGCCCTCCCCAGCTTTCCACAGTCCTCACCCTCCTTCCGAGGTCACTGCATCCATTGT
GAAAACTAACTCACATGAACCCGGAAAGCGTGAAAAGAGAACATTGGTTCTTAGAGACCG
AAATCTCTCTGGCATGGCTGAAAGGAAGACCCAGCCGAGCATGGTGAGTAATCCAGGCAG
CTGCAGCGACCCCCAGCCCAGCCCCGAGATGAAGCCGCATTCCTACCTGGATGCCATCAG
GAGTGGCCTTGATGACGTGGAGGCCAGCAGCTCCTACAGCAACGAGCAGCAGCTGTGCCC
CTACGCAGCTGCTGGGGAGTGCCGGTTTGGGGATGCCTGTGTCTACCTGCACGGGGAGGT
GTGTGAAATCTGTAGGCTGCAAGTCTTGCACCCATTCGACCCAGAGCAGAGGAAGGCTCA
CGAAAAGATCTGCATGTTGACGTTCGAACACGAGATGGAAAAGGCCTTTGCCTTCCAGGC
AAACCAGGACAAAGTGTGCAGTATCTGCATGGAAGTGATCCTGGAGAAGGCCTCTGCTTC
TGAGAGGAGATTTGGGATTCTCTCCAATTGCAATCACACGTACTGTTTGTCCTGCATCCG
GCAGTGGCGGTGTGCCAAACAGTTTGAAAACCCAATCATTAAGTCTTGTCCAGAATGCCG
TGTGATATCAGAGTTTGTAATTCCAAGTGTGTATTGGGTGGAAGATCAGAATAAAAAGAA
CGAGTTGATTGAAGCTTTCAAACAGGGGATGGGGAAAAAAGCCTGTAAATACTTTGAGCA
AGGCAAGGGGACCTGCCCATTTGGAAGCAAATGTCTTTATCGCCATGCTTACCCCGATGG
GCGGCTAGCAGAGCCTGAGAAACCTCGGAAACAGCTCAGTTCTCAAGGCACTGTGAGGTT
CTTTAATTCAGTGCGGCTCTGGGATTTCATCGAGAACCGAGAAAGCCGGCATGTCCCCAA
CAATGAAGATGTCGACATGACAGAGCTCGGGGACCTCTTCATGCACCTTTCTGGAGTGGA
ATCATCAGAACCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT