(Linear) (Six-base) MAPSORT of: KIEE3912.seq Check: 5825 from: 1 to: 1248 KIEE3912.orf With 182 enzymes: SgfI * March 22, 2012 16:49 .. AccI GT'mk_AC Cuts at: 0 557 1187 1248 Size: 557 630 61 Fragments arranged by size: 630 557 61 AflIII A'CryG_T Cuts at: 0 791 1248 Size: 791 457 AlwNI CAG_nnn'CTG Cuts at: 0 507 526 719 1069 1248 Size: 507 19 193 350 179 Fragments arranged by size: 507 350 193 179 19 AvaI C'yCGr_G Cuts at: 0 418 1201 1248 Size: 418 783 47 Fragments arranged by size: 783 418 47 BanI G'GyrC_C Cuts at: 0 199 1248 Size: 199 1049 BanII G_rGCy'C Cuts at: 0 1202 1248 Size: 1202 46 BbsI GAAGACnn'nnnn_ Cuts at: 0 367 1248 Size: 367 881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 244 278 1248 Size: 244 34 970 Fragments arranged by size: 970 244 34 BfrBI ATG'CAT Cuts at: 0 36 1248 Size: 36 1212 BglI GCCn_nnn'nGGC Cuts at: 0 691 1248 Size: 691 557 BglII A'GATC_T Cuts at: 0 641 1248 Size: 641 607 Bme1580I G_kGCm'C Cuts at: 0 202 221 514 1248 Size: 202 19 293 734 Fragments arranged by size: 734 293 202 19 BmgBI CAC'GTC Cuts at: 0 471 1248 Size: 471 777 BmtI G_CTAG'C Cuts at: 0 1063 1248 Size: 1063 185 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 647 762 1248 Size: 647 115 486 Fragments arranged by size: 647 486 115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 757 1247 1248 Size: 206 551 490 1 Fragments arranged by size: 551 490 206 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1082 1248 Size: 1082 166 BsaI GGTCTCn'nnnn_ Cuts at: 0 323 1248 Size: 323 925 BsaAI yAC'GTr Cuts at: 0 794 1248 Size: 794 454 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 179 209 523 553 1248 Size: 179 30 314 30 695 Fragments arranged by size: 695 314 179 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 774 1248 Size: 774 474 BseYI C'CCAG_C Cuts at: 0 225 365 407 412 526 1248 Size: 225 140 42 5 114 722 Fragments arranged by size: 722 225 140 114 42 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 171 547 732 1248 Size: 171 376 185 516 Fragments arranged by size: 516 376 185 171 BsiHKAI G_wGCw'C Cuts at: 0 7 1202 1248 Size: 7 1195 46 Fragments arranged by size: 1195 46 7 BsmI GAATG_Cn' Cuts at: 0 432 874 1248 Size: 432 442 374 Fragments arranged by size: 442 432 374 Bsp1286I G_dGCh'C Cuts at: 0 7 202 221 514 1202 1248 Size: 7 195 19 293 688 46 Fragments arranged by size: 688 293 195 46 19 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 14 569 1015 1248 Size: 14 555 446 233 Fragments arranged by size: 555 446 233 14 BsrFI r'CCGG_y Cuts at: 0 536 1160 1248 Size: 536 624 88 Fragments arranged by size: 624 536 88 BssSI C'ACGA_G Cuts at: 0 165 664 1248 Size: 165 499 584 Fragments arranged by size: 584 499 165 BstAPI GCAn_nnn'nTGC Cuts at: 0 600 719 1248 Size: 600 119 529 Fragments arranged by size: 600 529 119 BstBI TT'CG_AA Cuts at: 0 659 1248 Size: 659 589 BstYI r'GATC_y Cuts at: 0 641 1248 Size: 641 607 BtgI C'CryG_G Cuts at: 0 203 1248 Size: 203 1045 BtsI GCAGTG_nn' Cuts at: 0 260 823 1248 Size: 260 563 425 Fragments arranged by size: 563 425 260 DraIII CAC_nnn'GTG Cuts at: 0 216 1248 Size: 216 1032 EarI CTCTTCn'nnn_ Cuts at: 0 1216 1248 Size: 1216 32 EcoICRI GAG'CTC Cuts at: 0 1200 1248 Size: 1200 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 757 1247 1248 Size: 206 551 490 1 Fragments arranged by size: 551 490 206 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 739 1248 Size: 739 509 EcoO109I rG'GnC_Cy Cuts at: 0 170 1004 1206 1248 Size: 170 834 202 42 Fragments arranged by size: 834 202 170 42 EcoRV GAT'ATC Cuts at: 0 881 1248 Size: 881 367 HincII GTy'rAC Cuts at: 0 653 1188 1248 Size: 653 535 60 Fragments arranged by size: 653 535 60 HindIII A'AGCT_T Cuts at: 0 947 1248 Size: 947 301 MfeI C'AATT_G Cuts at: 0 780 1248 Size: 780 468 MslI CAynn'nnrTG Cuts at: 0 727 1248 Size: 727 521 MspA1I CmG'CkG Cuts at: 0 184 395 507 523 1248 Size: 184 211 112 16 725 Fragments arranged by size: 725 211 184 112 16 NaeI GCC'GGC Cuts at: 0 1162 1248 Size: 1162 86 NcoI C'CATG_G Cuts at: 0 203 1248 Size: 203 1045 NgoMIV G'CCGG_C Cuts at: 0 1160 1248 Size: 1160 88 NheI G'CTAG_C Cuts at: 0 1059 1248 Size: 1059 189 NsiI A_TGCA'T Cuts at: 0 38 1248 Size: 38 1210 NspI r_CATG'y Cuts at: 0 651 1168 1248 Size: 651 517 80 Fragments arranged by size: 651 517 80 PfoI T'CCnGG_A Cuts at: 0 734 1248 Size: 734 514 PpuMI rG'GwC_Cy Cuts at: 0 1004 1206 1248 Size: 1004 202 42 Fragments arranged by size: 1004 202 42 PstI C_TGCA'G Cuts at: 0 183 400 1248 Size: 183 217 848 Fragments arranged by size: 848 217 183 PvuII CAG'CTG Cuts at: 0 184 395 507 523 1248 Size: 184 211 112 16 725 Fragments arranged by size: 725 211 184 112 16 SacI G_AGCT'C Cuts at: 0 1202 1248 Size: 1202 46 SalI G'TCGA_C Cuts at: 0 1186 1248 Size: 1186 62 ScaI AGT'ACT Cuts at: 0 115 1248 Size: 115 1133 SfcI C'TryA_G Cuts at: 0 179 396 489 585 1248 Size: 179 217 93 96 663 Fragments arranged by size: 663 217 179 96 93 SmlI C'TyrA_G Cuts at: 0 1097 1248 Size: 1097 151 StuI AGG'CCT Cuts at: 0 679 745 1248 Size: 679 66 503 Fragments arranged by size: 679 503 66 StyI C'CwwG_G Cuts at: 0 203 1248 Size: 203 1045 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 347 620 900 1248 Size: 347 273 280 348 Fragments arranged by size: 348 347 280 273 TatI w'GTAC_w Cuts at: 0 113 1248 Size: 113 1135 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 179 211 235 267 694 726 1248 Size: 179 32 24 32 427 32 522 Fragments arranged by size: 522 427 179 32 32 32 24 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 905 1248 Size: 905 343 Enzymes that do cut: AccI AflIII AlwNI AvaI BanI BanII BbsI BcgI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstBI BstYI BtgI BtsI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HincII HindIII MfeI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PfoI PpuMI PstI PvuII SacI SalI ScaI SfcI SmlI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BlpI BmrI Bpu10I BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoRI FalI FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI MluI MmeI MscI NarI NdeI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI