(Circular) (Six-base) MAPSORT of: pFN21AE3912.seq Check: 2654 from: 1 to: 5953 pFN21AE3912 5953 bp With 182 enzymes: SgfI * March 28, 2012 11:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2562 3192 3296 1937 Size: 625 630 104 4594 Fragments arranged by size: 4594 630 625 104 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4016 5400 5668 1632 Size: 2384 1384 268 1917 Fragments arranged by size: 2384 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 2796 4852 5829 1203 Size: 1593 2056 977 1327 Fragments arranged by size: 2056 1593 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AlwNI CAG_nnn'CTG Cuts at: 2512 2531 2724 3074 3696 5268 2512 Size: 19 193 350 622 1572 3197 Fragments arranged by size: 3197 1572 622 350 193 19 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 3262 3488 3640 5749 1493 Size: 1769 226 152 2109 1697 Fragments arranged by size: 2109 1769 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 2423 3206 3278 1870 Size: 73 480 783 72 4545 Fragments arranged by size: 4545 783 480 73 72 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 3274 4729 618 Size: 325 217 627 417 1070 1455 1842 Fragments arranged by size: 1842 1455 1070 627 417 325 217 BanII G_rGCy'C Cuts at: 1391 3207 3272 1391 Size: 1816 65 4072 Fragments arranged by size: 4072 1816 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2372 928 Size: 971 473 4509 Fragments arranged by size: 4509 971 473 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2249 2283 4144 4178 1903 Size: 34 312 34 1861 34 3678 Fragments arranged by size: 3678 1861 312 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 1548 Size: 5953 BfrBI ATG'CAT Cuts at: 2041 2041 Size: 5953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2696 4564 136 Size: 107 122 71 631 605 1024 1868 1525 Fragments arranged by size: 1868 1525 1024 631 605 122 107 71 BglII A'GATC_T Cuts at: 2646 5948 2646 Size: 3302 2651 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 2207 2226 2519 4014 5170 1790 Size: 417 19 293 1495 1156 2573 Fragments arranged by size: 2573 1495 1156 417 293 19 BmgBI CAC'GTC Cuts at: 1318 2476 1318 Size: 1158 4795 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3684 4642 442 Size: 416 53 83 399 24 2267 958 1753 Fragments arranged by size: 2267 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3068 1056 Size: 2012 3941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2652 2767 2652 Size: 115 5838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2211 2762 3252 4613 1239 Size: 972 551 490 1361 2579 Fragments arranged by size: 2579 1361 972 551 490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3087 4078 4943 5241 3087 Size: 991 865 298 3799 Fragments arranged by size: 3799 991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2328 4616 882 Size: 617 829 2288 2219 Fragments arranged by size: 2288 2219 829 617 BsaAI yAC'GTr Cuts at: 493 1204 2799 3744 493 Size: 711 1595 945 2702 Fragments arranged by size: 2702 1595 945 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3760 4142 4777 275 Size: 53 83 186 936 2227 382 635 1451 Fragments arranged by size: 2227 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 2528 2558 1178 Size: 30 165 30 781 30 314 30 4573 Fragments arranged by size: 4573 781 314 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2779 1186 Size: 1593 4360 BseYI C'CCAG_C Cuts at: 2230 2370 2412 2417 2531 5156 5652 2230 Size: 140 42 5 114 2625 496 2531 Fragments arranged by size: 2625 2531 496 140 114 42 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2176 2552 2737 1163 Size: 573 440 376 185 4379 Fragments arranged by size: 4379 573 440 376 185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3403 4164 5192 1540 Size: 275 186 1402 761 1028 2301 Fragments arranged by size: 2301 1402 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 3207 4014 4099 5170 2012 Size: 1195 807 85 1071 2795 Fragments arranged by size: 2795 1195 1071 807 85 BsmI GAATG_Cn' Cuts at: 2437 2879 3464 3557 2437 Size: 442 585 93 4833 Fragments arranged by size: 4833 585 442 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2207 2226 2519 3207 3272 4014 Size: 399 222 195 19 293 688 65 742 Cuts at: 4014 4099 5170 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 688 399 293 222 195 85 65 19 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2019 2574 3020 3308 844 Size: 1175 555 446 288 3489 Fragments arranged by size: 3489 1175 555 446 288 BsrBI CCG'CTC Cuts at: 1394 1823 3840 1394 Size: 429 2017 3507 Fragments arranged by size: 3507 2017 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4448 4622 65 Size: 1183 3200 174 1396 Fragments arranged by size: 3200 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2541 3165 4597 4784 1700 Size: 110 731 624 1432 187 2869 Fragments arranged by size: 2869 1432 731 624 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 2170 2669 4013 5025 2170 Size: 499 1344 1012 3098 Fragments arranged by size: 3098 1344 1012 499 BstAPI GCAn_nnn'nTGC Cuts at: 794 2605 2724 794 Size: 1811 119 4023 Fragments arranged by size: 4023 1811 119 BstBI TT'CG_AA Cuts at: 2664 4757 2664 Size: 2093 3860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstYI r'GATC_y Cuts at: 1972 2646 3283 3650 4035 4052 5504 5590 5602 Size: 674 637 367 385 17 1452 86 12 Cuts at: 5602 5948 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 674 637 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2208 513 Size: 552 1143 4258 Fragments arranged by size: 4258 1143 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 2265 2828 3469 4263 4283 2265 Size: 563 641 794 20 3935 Fragments arranged by size: 3935 794 641 563 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DraIII CAC_nnn'GTG Cuts at: 2221 2221 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3400 4289 5918 Size: 54 1425 50 275 18 1570 889 1629 Cuts at: 5918 8 Size: 43 Fragments arranged by size: 1629 1570 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3221 3883 1353 Size: 1868 662 3423 Fragments arranged by size: 3423 1868 662 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3205 3205 Size: 5953 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2762 3252 4016 4613 5400 5668 Size: 393 579 551 490 764 597 787 268 Cuts at: 5668 1239 Size: 1524 Fragments arranged by size: 1524 787 764 597 579 551 490 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2744 2744 Size: 5953 EcoO109I rG'GnC_Cy Cuts at: 2175 3009 3211 2175 Size: 834 202 4917 Fragments arranged by size: 4917 834 202 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 EcoRV GAT'ATC Cuts at: 2886 2886 Size: 5953 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 5100 1678 Size: 3422 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4594 4626 4668 4700 1373 Size: 32 135 32 3022 32 42 32 2626 Fragments arranged by size: 3022 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2658 3193 3297 3543 669 Size: 988 281 720 535 104 246 3079 Fragments arranged by size: 3079 988 720 535 281 246 104 HindIII A'AGCT_T Cuts at: 748 2952 748 Size: 2204 3749 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 2785 3552 2785 Size: 767 5186 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5067 5251 1305 Size: 3762 184 2007 Fragments arranged by size: 3762 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4131 Fragments arranged by size: 4131 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 3911 4270 4429 518 Size: 648 1566 1179 359 159 2042 Fragments arranged by size: 2042 1566 1179 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2189 2400 2512 2528 3369 4046 5194 5439 Size: 583 211 112 16 841 677 1148 245 Cuts at: 5439 1606 Size: 2120 Fragments arranged by size: 2120 1148 841 677 583 245 211 112 16 NaeI GCC'GGC Cuts at: 3167 3167 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2208 513 Size: 552 1143 4258 Fragments arranged by size: 4258 1143 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NgoMIV G'CCGG_C Cuts at: 3165 3165 Size: 5953 NheI G'CTAG_C Cuts at: 1052 3064 1052 Size: 2012 3941 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NsiI A_TGCA'T Cuts at: 2043 2043 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2656 3173 3311 4856 1132 Size: 97 1427 517 138 1545 2229 Fragments arranged by size: 2229 1545 1427 517 138 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5953 PfoI T'CCnGG_A Cuts at: 2739 2739 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3009 3211 3009 Size: 202 5751 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PstI C_TGCA'G Cuts at: 830 2188 2405 3305 830 Size: 1358 217 900 3478 Fragments arranged by size: 3478 1358 900 217 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 PvuII CAG'CTG Cuts at: 2189 2400 2512 2528 2189 Size: 211 112 16 5614 Fragments arranged by size: 5614 211 112 16 SacI G_AGCT'C Cuts at: 3207 3207 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3191 3295 1936 Size: 1255 104 4594 Fragments arranged by size: 4594 1255 104 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 2120 4201 5913 2120 Size: 2081 1712 2160 Fragments arranged by size: 2160 2081 1712 SfcI C'TryA_G Cuts at: 826 1045 2184 2401 2494 2590 3301 4436 5117 Size: 219 1139 217 93 96 711 1135 681 Cuts at: 5117 5308 826 Size: 191 1471 Fragments arranged by size: 1471 1139 1135 711 681 219 217 191 96 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3102 4057 4958 5220 820 Size: 197 926 1159 955 901 262 1553 Fragments arranged by size: 1553 1159 955 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 2684 2750 1517 Size: 1167 66 4720 Fragments arranged by size: 4720 1167 66 StyI C'CwwG_G Cuts at: 513 1065 2208 3392 513 Size: 552 1143 1184 3074 Fragments arranged by size: 3074 1184 1143 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2352 2625 2905 3980 3997 4150 4335 Size: 1255 772 273 280 1075 17 153 185 Cuts at: 4335 4780 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1075 772 445 280 273 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 4199 5911 Size: 274 80 33 51 1443 141 2081 1712 Cuts at: 5911 96 Size: 138 Fragments arranged by size: 2081 1712 1443 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 2240 2272 2699 2731 5913 5945 2184 Size: 32 24 32 427 32 3182 32 2192 Fragments arranged by size: 3182 2192 427 32 32 32 32 24 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2910 2910 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 4082 4082 Size: 5953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI