################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NarI NdeI NotI NruI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 791 ACACGT A^C[GA][CT]GT AlwI 732 GATCC GGATC(4/5) BanI 199 GGCACC G^G[CT][GA]CC BanII 1198 GAGCTC G[GA]GC[CT]^C BbsI 360 GAAGAC GAAGAC(2/6) BcgI 255 CGAGGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 295 CCCGG CC^[GC]GG BfaI 1060 CTAG C^TAG BglI 685 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 641 AGATCT A^GATCT BpuAI 360 GAAGAC GAAGAC(2/6) BsaAI 792 CACGTA [CT]AC^GT[GA] BsaI 329 GAGACC GGTCTC(1/5) BseRI 759 GAGGAG GAGGAG(10/8) BsmAI 329 GAGAC GTCTC(1/5) BstBI 658 TTCGAA TT^CGAA BstYI 641 AGATCT [GA]^GATC[CT] DraIII 211 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 203 CCATGG C^C[GA][CT]GG EarI 1210 CTCTTC CTCTTC(1/4) EcoNI 735 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 879 GATATC GAT^ATC EcoT14I 203 CCATGG C^C[AT][AT]GG EcoT22I 34 ATGCAT ATGCA^T HindIII 947 AAGCTT A^AGCTT Ksp632I 1210 CTCTTC CTCTTC(1/4) MaeI 1060 CTAG C^TAG MaeIII 259 GTCAC ^GT[ACGT]AC MfeI 780 CAATTG C^AATTG MflI 641 AGATCT [GA]^GATC[CT] MslI 723 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 780 CAATTG C^AATTG NaeI 1160 GCCGGC GCC^GGC NciI 295 CCCGG CC^[GC]GG NcoI 203 CCATGG C^CATGG NgoMIV 1160 GCCGGC G^CCGGC NheI 1059 GCTAGC G^CTAGC NsiI 34 ATGCAT ATGCA^T NspV 658 TTCGAA TT^CGAA SacI 1198 GAGCTC GAGCT^C SalI 1186 GTCGAC G^TCGAC ScaI 113 AGTACT AGT^ACT SfuI 658 TTCGAA TT^CGAA SmlI 1097 CTCAAG C^T[CT][GA]AG StyI 203 CCATGG C^C[AT][AT]GG Tsp45I 259 GTCAC ^GT[GC]AC XcmI 898 CCAAGTGTGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 641 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 556 GTCTAC GT^[AC][GT]AC 1186 GTCGAC AfaI 114 GTAC GT^AC 795 GTAC AspHI 3 GAGCAC G[AT]GC[AT]^C 1198 GAGCTC AvaI 418 CCCGAG C^[CT]CG[GA]G 1201 CTCGGG AvaII 1004 GGACC G^G[AT]CC 1206 GGACC BsiHKAI 3 GAGCAC G[AT]GC[AT]^C 1198 GAGCTC BsmI 868 GAATGC GAATGC(1/-1) 432 GCATTC BsoBI 418 CCCGAG C^[CT]CG[GA]G 1201 CTCGGG BsrFI 536 GCCGGT [GA]^CCGG[CT] 1160 GCCGGC BssSI 165 CACGAG CACGAG(-5/-1) 664 CACGAG BstAPI 594 GCAAGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 713 GCAGTATCTGC Cfr10I 536 GCCGGT [GA]^CCGG[CT] 1160 GCCGGC HincII 651 GTTGAC GT[CT]^[GA]AC 1186 GTCGAC HindII 651 GTTGAC GT[CT]^[GA]AC 1186 GTCGAC HinfI 771 GATTC G^A[ACGT]TC 1234 GAATC HphI 378 GGTGA GGTGA(8/7) 243 TCACC MseI 854 TTAA T^TAA 1118 TTAA NspI 647 GCATGT [GA]CATG^[CT] 1164 GCATGT PpuMI 1003 GGGACCT [GA]G^G[AT]CC[CT] 1205 GGGACCT PstI 179 CTGCAG CTGCA^G 396 CTGCAG RsaI 114 GTAC GT^AC 795 GTAC StuI 677 AGGCCT AGG^CCT 743 AGGCCT TfiI 771 GATTC G^A[AT]TC 1234 GAATC Tru9I 854 TTAA T^TAA 1118 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AluI AlwNI BbvI BmyI BpmI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp509I TspRI TthHB8I