KLHL21 ORF clone pFN21AE3256

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3256

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Clone info.

Species

human

Clone Name

pFN21AE3256 Sequence (with vector) Restriction Map

Product ID

FHC07649

ORF Sequence

DNA sequence (1791 bp)
Protein sequence (597 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3256

Symbol

KLHL21

Description

kelch-like family member 21

Original Clone ID

cp93256

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1791 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 597 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS30575.1 0 100.0 KLHL21 CCDS46555.2 3.4e-70 39.1 KLHL30 CCDS3246.1 2.3e-63 34.3 KLHL24 CCDS30550.1 1.9e-48 33.9 KLHL17 CCDS1310.1 1.4e-47 33.0 KLHL20 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 25 133 PF00651 BTB/POZ IPR011705 138 239 PF07707 BTB/Kelch-associated IPR006652 288 321 PF01344 Kelch repeat type 1 IPR006652 327 368 PF01344 Kelch repeat type 1 IPR006652 371 408 PF01344 Kelch repeat type 1 IPR006652 504 545 PF01344 Kelch repeat type 1 HMMSmart IPR000210 35 133 SM00225 BTB/POZ-like IPR011705 138 239 SM00875 BTB/Kelch-associated IPR006652 287 334 SM00612 Kelch repeat type 1 IPR006652 336 382 SM00612 Kelch repeat type 1 IPR006652 383 422 SM00612 Kelch repeat type 1 IPR006652 471 512 SM00612 Kelch repeat type 1 IPR006652 513 560 SM00612 Kelch repeat type 1 ProfileScan IPR000210 35 103 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055666 597 616262 100.0 100.0 NP_001311238 500 616262 100.0 83.8 XP_016862148 600 611295 34.3 91.8 XP_005247610 600 611295 34.3 91.8 XP_005247611 600 611295 34.3 91.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3256 Download

>KIEE3256  1791 bp
ATGGAGCGACCGGCGCCCCTGGCCGTGCTTCCCTTCTCGGACCCCGCGCACGCCCTGAGC
CTGCTGCGCGGCCTGAGCCAGCTGCGCGCCGAGCGCAAGTTCCTGGACGTGACCCTGGAG
GCGGCGGGCGGGCGCGACTTCCCGGCGCACCGTGCGGTGCTGGCCGCCGCCAGCCCCTAC
TTCCGCGCCATGTTCGCGGGGCAGCTGCGCGAGAGCCGCGCCGAGCGGGTGCGCCTGCAC
GGAGTGCCTCCCGACATGCTGCAGCTGCTGCTGGACTTCAGCTACACGGGCCGCGTGGCG
GTAAGCGGCGACAACGCTGAGCCGCTGCTGCGCGCCGCCGACCTGCTGCAGTTCCCGGCC
GTGAAGGAGGCGTGCGGGGCCTTCCTGCAGCAGCAGCTCGACCTGGCCAACTGCCTGGAC
ATGCAGGACTTCGCTGAGGCCTTCAGCTGCTCGGGACTGGCGAGCGCGGCGCAGCGGTTC
ATTCTGCGCCACGTGGGCGAGCTGGGCGCCGAGCAGCTGGAGCGGCTGCCACTGGCGCGC
CTGCTGCGCTACCTGCGGGACGACGGGCTGTGTGTGCCCAAGGAGGAGGCCGCCTACCAG
CTGGCGCTGCGCTGGGTCCGCGCTGACCCGCCGCGCCGCGCCGCGCACTGGCCGCAGCTG
CTGGAGGCCGTGCGCCTGCCCTTCGTGCGCCGCTTCTACCTGTTGGCGCACGTCGAGGCC
GAGCCGCTGGTGGCGCGCTGCCCACCCTGCCTGCGCCTGCTGCGCGAGGCGCGCGACTTC
CAGGCGGCGCGCTACGACCGCCACGACCGCGGGCCCTGTCCCCGAATGCGTCCTCGCCCG
TCCACCGGTCTCGCCGAGATCCTCGTGCTCGTGGGCGGCTGCGACCAGGACTGTGACGAG
CTGGTCACTGTCGACTGCTACAACCCGCAGACGGGTCAGTGGCGCTACCTGGCCGAGTTC
CCAGACCACCTGGGCGGAGGCTACAGCATCGTGGCGCTGGGCAATGACATCTACGTGACG
GGTGGGTCCGATGGCTCCCGGCTCTATGACTGCGTGTGGAGGTACAACTCAAGCGTGAAT
GAGTGGGCGGAGGTGGCGCCCATGCTGAAGGCCCGCGAGTACCACAGCTCCTCTGTGCTG
GACGGACTGCTGTACGTGGTGGCCGCCGACAGCACCGAGCGCTATGACCACACCACTGAC
TCCTGGGAGGCCCTGCAGCCCATGACCTACCCCATGGACAACTGCTCCACCACTGCGTGC
CGTGGCCGGCTCTATGCCATCGGCTCCCTGGCTGGCAAGGAGACCATGGTGATGCAGTGC
TACGACCCGGACACCGACCTGTGGTCGCTGGTGGACTGCGGCCAGCTCCCGCCCTGGTCC
TTCGCCCCCAAGACTGCGACTCTAAACGGACTCATGTACTTTGTCAGGGATGACTCCGCT
GAGGTGGACGTGTACAACCCGACGAGGAACGAATGGGACAAGATCCCGTCCATGAATCAG
GTACATGTGGGGGGCAGCCTGGCCGTCCTTGGGGGGAAGCTGTACGTCTCTGGGGGATAC
GACAATACATTTGAACTCTCGGACGTGGTAGAGGCCTATGACCCAGAGACTCGCGCGTGG
AGCGTGGTGGGGCGGCTCCCAGAACCCACCTTCTGGCATGGCAGTGTCAGCATCTTCCGC
CAGTTCATGCCCCAGACCTTCTCGGGTGGGCGTGGCTTCGAGTTGGACAGTGGCAGCGAT
GACATGGACCCAGGCCGACCCCGGCCGCCGCGGGACCCCGATGAGCTGCAC

ORF Protein

Cloned ORF protein sequence for pFN21AE3256 Download

>KIEE3256  597 aa
MERPAPLAVLPFSDPAHALSLLRGLSQLRAERKFLDVTLEAAGGRDFPAHRAVLAAASPY
FRAMFAGQLRESRAERVRLHGVPPDMLQLLLDFSYTGRVAVSGDNAEPLLRAADLLQFPA
VKEACGAFLQQQLDLANCLDMQDFAEAFSCSGLASAAQRFILRHVGELGAEQLERLPLAR
LLRYLRDDGLCVPKEEAAYQLALRWVRADPPRRAAHWPQLLEAVRLPFVRRFYLLAHVEA
EPLVARCPPCLRLLREARDFQAARYDRHDRGPCPRMRPRPSTGLAEILVLVGGCDQDCDE
LVTVDCYNPQTGQWRYLAEFPDHLGGGYSIVALGNDIYVTGGSDGSRLYDCVWRYNSSVN
EWAEVAPMLKAREYHSSSVLDGLLYVVAADSTERYDHTTDSWEALQPMTYPMDNCSTTAC
RGRLYAIGSLAGKETMVMQCYDPDTDLWSLVDCGQLPPWSFAPKTATLNGLMYFVRDDSA
EVDVYNPTRNEWDKIPSMNQVHVGGSLAVLGGKLYVSGGYDNTFELSDVVEAYDPETRAW
SVVGRLPEPTFWHGSVSIFRQFMPQTFSGGRGFELDSGSDDMDPGRPRPPRDPDELH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3256 Download

>pFN21AE3256  6496 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCGACCGGCGCCCCTGGCCGTGCTTCCCTT
CTCGGACCCCGCGCACGCCCTGAGCCTGCTGCGCGGCCTGAGCCAGCTGCGCGCCGAGCG
CAAGTTCCTGGACGTGACCCTGGAGGCGGCGGGCGGGCGCGACTTCCCGGCGCACCGTGC
GGTGCTGGCCGCCGCCAGCCCCTACTTCCGCGCCATGTTCGCGGGGCAGCTGCGCGAGAG
CCGCGCCGAGCGGGTGCGCCTGCACGGAGTGCCTCCCGACATGCTGCAGCTGCTGCTGGA
CTTCAGCTACACGGGCCGCGTGGCGGTAAGCGGCGACAACGCTGAGCCGCTGCTGCGCGC
CGCCGACCTGCTGCAGTTCCCGGCCGTGAAGGAGGCGTGCGGGGCCTTCCTGCAGCAGCA
GCTCGACCTGGCCAACTGCCTGGACATGCAGGACTTCGCTGAGGCCTTCAGCTGCTCGGG
ACTGGCGAGCGCGGCGCAGCGGTTCATTCTGCGCCACGTGGGCGAGCTGGGCGCCGAGCA
GCTGGAGCGGCTGCCACTGGCGCGCCTGCTGCGCTACCTGCGGGACGACGGGCTGTGTGT
GCCCAAGGAGGAGGCCGCCTACCAGCTGGCGCTGCGCTGGGTCCGCGCTGACCCGCCGCG
CCGCGCCGCGCACTGGCCGCAGCTGCTGGAGGCCGTGCGCCTGCCCTTCGTGCGCCGCTT
CTACCTGTTGGCGCACGTCGAGGCCGAGCCGCTGGTGGCGCGCTGCCCACCCTGCCTGCG
CCTGCTGCGCGAGGCGCGCGACTTCCAGGCGGCGCGCTACGACCGCCACGACCGCGGGCC
CTGTCCCCGAATGCGTCCTCGCCCGTCCACCGGTCTCGCCGAGATCCTCGTGCTCGTGGG
CGGCTGCGACCAGGACTGTGACGAGCTGGTCACTGTCGACTGCTACAACCCGCAGACGGG
TCAGTGGCGCTACCTGGCCGAGTTCCCAGACCACCTGGGCGGAGGCTACAGCATCGTGGC
GCTGGGCAATGACATCTACGTGACGGGTGGGTCCGATGGCTCCCGGCTCTATGACTGCGT
GTGGAGGTACAACTCAAGCGTGAATGAGTGGGCGGAGGTGGCGCCCATGCTGAAGGCCCG
CGAGTACCACAGCTCCTCTGTGCTGGACGGACTGCTGTACGTGGTGGCCGCCGACAGCAC
CGAGCGCTATGACCACACCACTGACTCCTGGGAGGCCCTGCAGCCCATGACCTACCCCAT
GGACAACTGCTCCACCACTGCGTGCCGTGGCCGGCTCTATGCCATCGGCTCCCTGGCTGG
CAAGGAGACCATGGTGATGCAGTGCTACGACCCGGACACCGACCTGTGGTCGCTGGTGGA
CTGCGGCCAGCTCCCGCCCTGGTCCTTCGCCCCCAAGACTGCGACTCTAAACGGACTCAT
GTACTTTGTCAGGGATGACTCCGCTGAGGTGGACGTGTACAACCCGACGAGGAACGAATG
GGACAAGATCCCGTCCATGAATCAGGTACATGTGGGGGGCAGCCTGGCCGTCCTTGGGGG
GAAGCTGTACGTCTCTGGGGGATACGACAATACATTTGAACTCTCGGACGTGGTAGAGGC
CTATGACCCAGAGACTCGCGCGTGGAGCGTGGTGGGGCGGCTCCCAGAACCCACCTTCTG
GCATGGCAGTGTCAGCATCTTCCGCCAGTTCATGCCCCAGACCTTCTCGGGTGGGCGTGG
CTTCGAGTTGGACAGTGGCAGCGATGACATGGACCCAGGCCGACCCCGGCCGCCGCGGGA
CCCCGATGAGCTGCACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT