################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII Alw44I AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbsI BcgI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BseAI BsiWI BspDI BspEI BspHI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 910 GTCGAC GT^[AC][GT]AC AgeI 844 ACCGGT A^CCGGT AhdI 1336 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1178 AGCGCT AGC^GCT ApaI 811 GGGCCC GGGCC^C AscI 534 GGCGCGCC GG^CGCGCC AspEI 1336 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 865 GTGCTC G[AT]GC[AT]^C BalI 404 TGGCCA TGG^CCA BanII 811 GGGCCC G[GA]GC[CT]^C BbrPI 490 CACGTG CAC^GTG BciVI 1555 GGATAC GTATCC(6/5) BglI 488 GCCACGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 316 GCTGAGC GC^T[ACGT]AGC Bpu1102I 316 GCTGAGC GC^T[ACGT]AGC BsaWI 844 ACCGGT [AT]^CCGG[AT] BsiHKAI 865 GTGCTC G[AT]GC[AT]^C BsmBI 1545 CGTCTC CGTCTC(1/5) BsmI 824 GAATGC GAATGC(1/-1) Bsp1407I 1451 TGTACA T^GTACA BspLU11I 1503 ACATGT A^CATGT BsrDI 1001 GCAATG GCAATG(2/0) BsrGI 1451 TGTACA T^GTACA BstF5I 1428 GGATG GGATG(2/0) BstXI 407 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 316 GCTGAGC GC^T[ACGT]AGC Eam1105I 1336 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1178 AGCGCT AGC^GCT FokI 1428 GGATG GGATG(9/13) HgaI 828 GCGTC GACGC(5/10) HincII 910 GTCGAC GT[CT]^[GA]AC HindII 910 GTCGAC GT[CT]^[GA]AC HphI 1308 GGTGA GGTGA(8/7) MboII 1673 TCTTC GAAGA(8/7) MluNI 404 TGGCCA TGG^CCA MscI 404 TGGCCA TGG^CCA NaeI 1265 GCCGGC GCC^GGC NgoMIV 1265 GCCGGC G^CCGGC PflMI 1646 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 844 ACCGGT A^CCGGT PmaCI 490 CACGTG CAC^GTG PmlI 490 CACGTG CAC^GTG PpuMI 1772 GGGACCC [GA]G^G[AT]CC[CT] SalI 910 GTCGAC G^TCGAC SmlI 1068 CTCAAG C^T[CT][GA]AG SspBI 1451 TGTACA T^GTACA TfiI 1494 GAATC G^A[AT]TC Van91I 1646 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1277 CCATCGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 1448 ACGTGT A^C[GA][CT]GT 1503 ACATGT AlwI 858 GATCC GGATC(4/5) 1482 GATCC AlwNI 262 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 655 CAGCTGCTG AvaI 450 CTCGGG C^[CT]CG[GA]G 1701 CTCGGG BbvCI 433 GCTGAGG CCTCAGC(-5/-2) 1438 GCTGAGG BsaI 1300 GAGACC GGTCTC(1/5) 847 GGTCTC BseRI 1128 CTCCTC GAGGAG(10/8) 583 GAGGAG BsgI 235 CTGCAC GTGCAG(16/14) 1786 CTGCAC BsoBI 450 CTCGGG C^[CT]CG[GA]G 1701 CTCGGG BspMI 341 ACCTGC ACCTGC(4/8) 551 ACCTGC BsrBI 223 GAGCGG CCGCTC(-3/-3) 520 GAGCGG BssSI 862 CTCGTG CACGAG(-5/-1) 868 CTCGTG BstAPI 237 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 261 GCAGCTGCTGC BstYI 857 AGATCC [GA]^GATC[CT] 1481 AGATCC DpnII 858 GATC ^GATC 1482 GATC DraIII 238 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1251 CACTGCGTG DrdI 895 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1017 GACGGGTGGGTC EagI 356 CGGCCG C^GGCCG 1762 CGGCCG EclXI 356 CGGCCG C^GGCCG 1762 CGGCCG Eco52I 356 CGGCCG C^GGCCG 1762 CGGCCG KspI 807 CCGCGG CCGC^GG 1768 CCGCGG MboI 858 GATC ^GATC 1482 GATC MflI 857 AGATCC [GA]^GATC[CT] 1481 AGATCC NcoI 1232 CCATGG C^CATGG 1304 CCATGG NdeII 858 GATC ^GATC 1482 GATC SacII 807 CCGCGG CCGC^GG 1768 CCGCGG Sau3AI 858 GATC ^GATC 1482 GATC StuI 437 AGGCCT AGG^CCT 1592 AGGCCT XhoII 857 AGATCC [GA]^GATC[CT] 1481 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BanI BbeI BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI TseI Tsp45I TspRI TthHB8I