(Circular) (Six-base) MAPSORT of: pFN21AE3256.seq Check: 6192 from: 1 to: 6496 pFN21AE3256 6496 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4306 278 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2916 3839 1937 Size: 979 923 4594 Fragments arranged by size: 4594 979 923 Acc65I G'GTAC_C Cuts at: 3817 3817 Size: 6496 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2266 2431 3131 4559 5943 6211 1632 Size: 634 165 700 1428 1384 268 1917 Fragments arranged by size: 1917 1428 1384 700 634 268 165 AfeI AGC'GCT Cuts at: 3185 3185 Size: 6496 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 3453 3508 5395 6372 1203 Size: 2250 55 1887 977 1327 Fragments arranged by size: 2250 1887 1327 977 55 AgeI A'CCGG_T Cuts at: 2849 5327 2849 Size: 2478 4018 AhdI GACnn_n'nnGTC Cuts at: 3346 5225 3346 Size: 1879 4617 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6496 AlwNI CAG_nnn'CTG Cuts at: 2272 2665 4239 5811 2272 Size: 393 1574 1572 2957 Fragments arranged by size: 2957 1574 1572 393 ApaI G_GGCC'C Cuts at: 2820 2820 Size: 6496 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 3805 4031 4183 6292 1493 Size: 2312 226 152 2109 1697 Fragments arranged by size: 2312 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2540 2540 Size: 6496 AseI AT'TA_AT Cuts at: 160 1032 5050 160 Size: 872 4018 1606 Fragments arranged by size: 4018 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 2455 3706 3821 1870 Size: 73 512 1251 115 4545 Fragments arranged by size: 4545 1251 512 115 73 BamHI G'GATC_C Cuts at: 3826 4193 3826 Size: 367 6129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2510 3100 3817 5272 Size: 325 217 627 230 493 590 717 1455 Cuts at: 5272 618 Size: 1842 Fragments arranged by size: 1842 1455 717 627 590 493 325 230 217 BanII G_rGCy'C Cuts at: 1391 2820 3815 1391 Size: 1429 995 4072 Fragments arranged by size: 4072 1429 995 BbeI G_GCGC'C Cuts at: 2021 2514 3104 2021 Size: 493 590 5413 Fragments arranged by size: 5413 590 493 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5525 BbvCI CC'TCA_GC Cuts at: 2439 3444 2439 Size: 1005 5491 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4687 4721 1903 Size: 34 2750 34 3678 Fragments arranged by size: 3678 2750 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4388 5598 3554 Size: 834 1210 4452 Fragments arranged by size: 4452 1210 834 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2499 5107 136 Size: 107 122 71 631 605 827 2608 1525 Fragments arranged by size: 2608 1525 827 631 605 122 107 71 BglII A'GATC_T Cuts at: 6491 6491 Size: 6496 BlpI GC'TnA_GC Cuts at: 2322 3913 2322 Size: 1591 4905 Bme1580I G_kGCm'C Cuts at: 1790 2583 2820 4557 5713 1790 Size: 793 237 1737 1156 2573 Fragments arranged by size: 2573 1737 1156 793 237 BmgBI CAC'GTC Cuts at: 1318 2113 2716 3454 3589 1318 Size: 795 603 738 135 4225 Fragments arranged by size: 4225 795 738 603 135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4227 5185 442 Size: 416 53 83 399 24 2810 958 1753 Fragments arranged by size: 2810 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2543 2687 5156 1239 Size: 902 402 144 2469 2579 Fragments arranged by size: 2579 2469 902 402 144 Bpu10I CC'TnA_GC Cuts at: 2060 2078 2439 3444 2060 Size: 18 361 1005 5112 Fragments arranged by size: 5112 1005 361 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3058 4621 5486 5784 3058 Size: 1563 865 298 3770 Fragments arranged by size: 3770 1563 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2858 3299 5159 882 Size: 617 1359 441 1860 2219 Fragments arranged by size: 2219 1860 1359 617 441 BsaAI yAC'GTr Cuts at: 493 1204 2497 3019 3160 4287 493 Size: 711 1293 522 141 1127 2702 Fragments arranged by size: 2702 1293 1127 711 522 141 BsaBI GATnn'nnATC Cuts at: 6490 6490 Size: 6496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2511 3101 4303 Size: 53 83 186 936 485 493 590 1202 Cuts at: 4303 4685 5320 275 Size: 382 635 1451 Fragments arranged by size: 1451 1202 936 635 590 493 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2849 4929 5327 5601 5748 6159 2849 Size: 2080 398 274 147 411 3186 Fragments arranged by size: 3186 2080 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3116 3146 3233 3263 1178 Size: 30 165 30 1713 30 87 30 4411 Fragments arranged by size: 4411 1713 165 87 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 3124 1186 Size: 1417 521 4558 Fragments arranged by size: 4558 1417 521 BseYI C'CCAG_C Cuts at: 2506 2616 3001 5699 6195 2506 Size: 110 385 2698 496 2807 Fragments arranged by size: 2807 2698 496 385 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 3776 1163 Size: 573 489 1551 3883 Fragments arranged by size: 3883 1551 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 2364 2803 2812 3770 3946 Size: 275 186 14 349 439 9 958 176 Cuts at: 3946 4707 5735 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 958 761 439 349 275 186 176 14 9 BsiHKAI G_wGCw'C Cuts at: 2874 4557 4642 5713 2874 Size: 1683 85 1071 3657 Fragments arranged by size: 3657 1683 1071 85 BsmI GAATG_Cn' Cuts at: 2835 4007 4100 2835 Size: 1172 93 5231 Fragments arranged by size: 5231 1172 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3556 3556 Size: 6496 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2583 2820 2874 3815 4557 4642 5713 Size: 399 793 237 54 941 742 85 1071 Cuts at: 5713 1391 Size: 2174 Fragments arranged by size: 2174 1071 941 793 742 399 237 85 54 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 4385 6115 4385 Size: 1730 4766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2355 2565 3851 844 Size: 1511 210 1286 3489 Fragments arranged by size: 3489 1511 1286 210 BsrBI CCG'CTC Cuts at: 1394 1823 2230 2527 4383 1394 Size: 429 407 297 1856 3507 Fragments arranged by size: 3507 1856 429 407 297 BsrDI GCAATG_nn' Cuts at: 65 1248 3013 4991 5165 65 Size: 1183 1765 1978 174 1396 Fragments arranged by size: 1978 1765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2849 3270 5140 5327 1700 Size: 110 204 835 421 1870 187 2869 Fragments arranged by size: 2869 1870 835 421 204 187 110 BsrGI T'GTAC_A Cuts at: 96 3456 96 Size: 3360 3136 BssHII G'CGCG_C Cuts at: 1925 2089 2335 2540 2738 2774 2792 1925 Size: 164 246 205 198 36 18 5629 Fragments arranged by size: 5629 246 205 198 164 36 18 BssSI C'ACGA_G Cuts at: 2867 2873 4556 5568 2867 Size: 6 1683 1012 3795 Fragments arranged by size: 3795 1683 1012 6 BstAPI GCAn_nnn'nTGC Cuts at: 794 2248 2272 794 Size: 1454 24 5018 Fragments arranged by size: 5018 1454 24 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2419 Size: 6496 BstYI r'GATC_y Cuts at: 1972 2862 3486 3826 4193 4578 4595 6047 6133 Size: 890 624 340 367 385 17 1452 86 Cuts at: 6133 6145 6491 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 890 624 385 367 346 340 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2812 3237 3265 3309 3773 513 Size: 552 939 808 425 28 44 464 3236 Fragments arranged by size: 3236 939 808 552 464 425 44 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3756 487 Size: 683 56 2530 3227 Fragments arranged by size: 3227 2530 683 56 BtsI GCAGTG_nn' Cuts at: 3255 3326 3673 4012 4806 4826 3255 Size: 71 347 339 794 20 4925 Fragments arranged by size: 4925 794 347 339 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3800 3800 Size: 6496 DraIII CAC_nnn'GTG Cuts at: 2248 3261 2248 Size: 1013 5483 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2906 3028 5503 809 Size: 507 1590 122 2475 1802 Fragments arranged by size: 2475 1802 1590 507 122 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2166 2361 Size: 54 1425 50 275 18 195 141 195 Cuts at: 2361 2409 2654 2955 3165 3268 3364 3525 3767 Size: 48 245 301 210 103 96 161 242 Cuts at: 3767 3943 4832 6461 8 Size: 176 889 1629 43 Fragments arranged by size: 1629 1425 889 301 275 245 242 210 195 195 176 161 141 103 96 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2361 3767 3943 1537 Size: 275 549 1406 176 4090 Fragments arranged by size: 4090 1406 549 275 176 EarI CTCTTCn'nnn_ Cuts at: 1353 4426 1353 Size: 3073 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2994 3107 3671 5083 5457 5603 Size: 1064 138 1405 113 564 1412 374 146 Cuts at: 5603 387 Size: 1280 Fragments arranged by size: 1412 1405 1280 1064 564 374 146 138 113 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2266 2431 2543 2687 3131 4559 Size: 393 509 125 165 112 144 444 1428 Cuts at: 4559 5156 5943 6211 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1428 787 597 509 444 393 268 165 144 125 112 EcoO109I rG'GnC_Cy Cuts at: 2382 2817 3214 3778 2382 Size: 435 397 564 5100 Fragments arranged by size: 5100 564 435 397 EcoRI G'AATT_C Cuts at: 3805 4183 3805 Size: 378 6118 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 2021 2514 2612 2950 3002 3104 3187 5643 Size: 343 493 98 338 52 102 83 2456 Cuts at: 5643 1678 Size: 2531 Fragments arranged by size: 2531 2456 493 343 338 102 98 83 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3233 3265 3332 3364 3388 Size: 32 135 32 1661 32 67 32 24 Cuts at: 3388 3420 5137 5169 5211 5243 1373 Size: 32 1717 32 42 32 2626 Fragments arranged by size: 2626 1717 1661 135 67 42 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2917 3840 4086 669 Size: 988 281 979 923 246 3079 Fragments arranged by size: 3079 988 979 923 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6496 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KasI G'GCGC_C Cuts at: 2017 2510 3100 2017 Size: 493 590 5413 Fragments arranged by size: 5413 590 493 KpnI G_GTAC'C Cuts at: 3821 3821 Size: 6496 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3708 5610 5794 1305 Size: 2403 1902 184 2007 Fragments arranged by size: 2403 2007 1902 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 10 Size: 54 1425 343 579 4095 Fragments arranged by size: 4095 1425 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 3314 3666 4454 4813 4972 518 Size: 648 1851 297 352 788 359 159 2042 Fragments arranged by size: 2042 1851 788 648 359 352 297 159 MspA1I CmG'CkG Cuts at: 1606 2086 2209 2269 2329 2451 2479 2521 2605 Size: 480 123 60 60 122 28 42 84 Cuts at: 2605 2662 2731 2814 3443 3775 3912 4589 5737 Size: 57 69 83 629 332 137 677 1148 Cuts at: 5737 5982 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 629 480 332 245 137 123 122 84 83 69 60 60 57 42 28 NaeI GCC'GGC Cuts at: 3272 3272 Size: 6496 NarI GG'CG_CC Cuts at: 2018 2511 3101 2018 Size: 493 590 5413 Fragments arranged by size: 5413 590 493 NcoI C'CATG_G Cuts at: 513 1065 2004 3237 3309 513 Size: 552 939 1233 72 3700 Fragments arranged by size: 3700 1233 939 552 72 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NgoMIV G'CCGG_C Cuts at: 3270 3270 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 2263 2428 3512 3854 5399 1132 Size: 97 1034 165 1084 342 1545 2229 Fragments arranged by size: 2229 1545 1084 1034 342 165 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 3508 5395 3508 Size: 1887 4609 PflMI CCAn_nnn'nTGG Cuts at: 1108 3657 1108 Size: 2549 3947 PfoI T'CCnGG_A Cuts at: 2106 2106 Size: 6496 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PmlI CAC'GTG Cuts at: 2497 2497 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2087 2119 4565 4597 6078 6110 2087 Size: 32 2446 32 1481 32 2473 Fragments arranged by size: 2473 2446 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3778 3778 Size: 6496 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 4066 4066 Size: 6496 PspOMI G'GGCC_C Cuts at: 2816 2816 Size: 6496 PstI C_TGCA'G Cuts at: 830 2268 2355 2394 3222 3848 830 Size: 1438 87 39 828 626 3478 Fragments arranged by size: 3478 1438 828 626 87 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 2086 2209 2269 2451 2521 2605 2662 2086 Size: 123 60 182 70 84 57 5920 Fragments arranged by size: 5920 182 123 84 70 60 57 SacII CC_GC'GG Cuts at: 2815 3776 2815 Size: 961 5535 SalI G'TCGA_C Cuts at: 1936 2915 3838 1936 Size: 979 923 4594 Fragments arranged by size: 4594 979 923 SanDI GG'GwC_CC Cuts at: 3778 3778 Size: 6496 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 4744 6456 4744 Size: 1712 4784 SfcI C'TryA_G Cuts at: 826 1045 2264 2351 2390 2986 3218 3844 4979 Size: 219 1219 87 39 596 232 626 1135 Cuts at: 4979 5660 5851 826 Size: 681 191 1471 Fragments arranged by size: 1471 1219 1135 681 626 596 232 219 191 87 39 SfoI GGC'GCC Cuts at: 2019 2512 3102 2019 Size: 493 590 5413 Fragments arranged by size: 5413 590 493 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 3823 1872 Size: 1951 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3073 4600 5501 5763 820 Size: 197 926 1130 1527 901 262 1553 Fragments arranged by size: 1553 1527 1130 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 3854 1132 Size: 2722 3774 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 2444 3599 1517 Size: 927 1155 4414 Fragments arranged by size: 4414 1155 927 StyI C'CwwG_G Cuts at: 513 1065 2004 2583 3237 3309 3532 3935 513 Size: 552 939 579 654 72 223 403 3074 Fragments arranged by size: 3074 939 654 579 552 403 223 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4523 4540 4693 4878 5323 325 Size: 1255 2943 17 153 185 445 1498 Fragments arranged by size: 2943 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3420 3456 4742 Size: 274 80 33 51 1443 1443 36 1286 Cuts at: 4742 6454 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1443 1286 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6456 6488 6456 Size: 32 6464 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3289 3289 Size: 6496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 3821 1870 Size: 1951 4545 XmnI GAAnn'nnTTC Cuts at: 4625 4625 Size: 6496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4304 276 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI