Restriction map for
pFN21AE3256
(Linear) (Six-base) MAPSORT of: KIEE3256.seq Check: 1202 from: 1 to: 1791
KIEE3256.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 911 1791
Size: 911 880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 426 1126 1791
Size: 261 165 700 665
Fragments arranged by size:
700 665 261 165
AfeI AGC'GCT
Cuts at: 0 1180 1791
Size: 1180 611
AflIII A'CryG_T
Cuts at: 0 1448 1503 1791
Size: 1448 55 288
Fragments arranged by size:
1448 288 55
AgeI A'CCGG_T
Cuts at: 0 844 1791
Size: 844 947
AhdI GACnn_n'nnGTC
Cuts at: 0 1341 1791
Size: 1341 450
AlwNI CAG_nnn'CTG
Cuts at: 0 267 660 1791
Size: 267 393 1131
Fragments arranged by size:
1131 393 267
ApaI G_GGCC'C
Cuts at: 0 815 1791
Size: 815 976
AscI GG'CGCG_CC
Cuts at: 0 535 1791
Size: 535 1256
AvaI C'yCGr_G
Cuts at: 0 450 1701 1791
Size: 450 1251 90
Fragments arranged by size:
1251 450 90
BanI G'GyrC_C
Cuts at: 0 12 505 1095 1791
Size: 12 493 590 696
Fragments arranged by size:
696 590 493 12
BanII G_rGCy'C
Cuts at: 0 815 1791
Size: 815 976
BbeI G_GCGC'C
Cuts at: 0 16 509 1099 1791
Size: 16 493 590 692
Fragments arranged by size:
692 590 493 16
BbvCI CC'TCA_GC
Cuts at: 0 434 1439 1791
Size: 434 1005 352
Fragments arranged by size:
1005 434 352
BciVI GTATCCnnnnn_n'
Cuts at: 0 1549 1791
Size: 1549 242
BglI GCCn_nnn'nGGC
Cuts at: 0 494 1791
Size: 494 1297
BlpI GC'TnA_GC
Cuts at: 0 317 1791
Size: 317 1474
Bme1580I G_kGCm'C
Cuts at: 0 578 815 1791
Size: 578 237 976
Fragments arranged by size:
976 578 237
BmgBI CAC'GTC
Cuts at: 0 108 711 1449 1584 1791
Size: 108 603 738 135 207
Fragments arranged by size:
738 603 207 135 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 538 682 1791
Size: 136 402 144 1109
Fragments arranged by size:
1109 402 144 136
Bpu10I CC'TnA_GC
Cuts at: 0 55 73 434 1439 1791
Size: 55 18 361 1005 352
Fragments arranged by size:
1005 361 352 55 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1053 1791
Size: 1053 738
BsaI GGTCTCn'nnnn_
Cuts at: 0 853 1294 1791
Size: 853 441 497
Fragments arranged by size:
853 497 441
BsaAI yAC'GTr
Cuts at: 0 492 1014 1155 1791
Size: 492 522 141 636
Fragments arranged by size:
636 522 492 141
BsaHI Gr'CG_yC
Cuts at: 0 13 506 1096 1791
Size: 13 493 590 695
Fragments arranged by size:
695 590 493 13
BsaWI w'CCGG_w
Cuts at: 0 844 1791
Size: 844 947
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1111 1141 1228 1258 1791
Size: 1111 30 87 30 533
Fragments arranged by size:
1111 533 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 1119 1791
Size: 598 521 672
Fragments arranged by size:
672 598 521
BseYI C'CCAG_C
Cuts at: 0 501 611 996 1791
Size: 501 110 385 795
Fragments arranged by size:
795 501 385 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1771 1791
Size: 220 1551 20
Fragments arranged by size:
1551 220 20
BsiEI CG_ry'CG
Cuts at: 0 10 359 798 807 1765 1791
Size: 10 349 439 9 958 26
Fragments arranged by size:
958 439 349 26 10 9
BsiHKAI G_wGCw'C
Cuts at: 0 869 1791
Size: 869 922
BsmI GAATG_Cn'
Cuts at: 0 830 1791
Size: 830 961
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1551 1791
Size: 1551 240
Bsp1286I G_dGCh'C
Cuts at: 0 578 815 869 1791
Size: 578 237 54 922
Fragments arranged by size:
922 578 237 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 560 1791
Size: 350 210 1231
Fragments arranged by size:
1231 350 210
BsrBI CCG'CTC
Cuts at: 0 225 522 1791
Size: 225 297 1269
Fragments arranged by size:
1269 297 225
BsrDI GCAATG_nn'
Cuts at: 0 1008 1791
Size: 1008 783
BsrFI r'CCGG_y
Cuts at: 0 9 844 1265 1791
Size: 9 835 421 526
Fragments arranged by size:
835 526 421 9
BsrGI T'GTAC_A
Cuts at: 0 1451 1791
Size: 1451 340
BssHII G'CGCG_C
Cuts at: 0 84 330 535 733 769 787 1791
Size: 84 246 205 198 36 18 1004
Fragments arranged by size:
1004 246 205 198 84 36 18
BssSI C'ACGA_G
Cuts at: 0 862 868 1791
Size: 862 6 923
Fragments arranged by size:
923 862 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 243 267 1791
Size: 243 24 1524
Fragments arranged by size:
1524 243 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1791
Size: 414 1377
BstYI r'GATC_y
Cuts at: 0 857 1481 1791
Size: 857 624 310
Fragments arranged by size:
857 624 310
BtgI C'CryG_G
Cuts at: 0 807 1232 1260 1304 1768 1791
Size: 807 425 28 44 464 23
Fragments arranged by size:
807 464 425 44 28 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1751 1791
Size: 1751 40
BtsI GCAGTG_nn'
Cuts at: 0 1250 1321 1668 1791
Size: 1250 71 347 123
Fragments arranged by size:
1250 347 123 71
DraIII CAC_nnn'GTG
Cuts at: 0 243 1256 1791
Size: 243 1013 535
Fragments arranged by size:
1013 535 243
DrdI GACnn_nn'nnGTC
Cuts at: 0 901 1023 1791
Size: 901 122 768
Fragments arranged by size:
901 768 122
EaeI y'GGCC_r
Cuts at: 0 20 161 356 404 649 950 1160 1263
Size: 20 141 195 48 245 301 210 103
Cuts at: 1263 1359 1520 1762 1791
Size: 96 161 242 29
Fragments arranged by size:
301 245 242 210 195 161 141 103
96 48 29 20
EagI C'GGCC_G
Cuts at: 0 356 1762 1791
Size: 356 1406 29
Fragments arranged by size:
1406 356 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 989 1102 1666 1791
Size: 989 113 564 125
Fragments arranged by size:
989 564 125 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 261 426 538 682 1126 1791
Size: 136 125 165 112 144 444 665
Fragments arranged by size:
665 444 165 144 136 125 112
EcoO109I rG'GnC_Cy
Cuts at: 0 377 812 1209 1773 1791
Size: 377 435 397 564 18
Fragments arranged by size:
564 435 397 377 18
HaeII r_GCGC'y
Cuts at: 0 16 509 607 945 997 1099 1182 1791
Size: 16 493 98 338 52 102 83 609
Fragments arranged by size:
609 493 338 102 98 83 52 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1228 1260 1327 1359 1383 1415 1791
Size: 1228 32 67 32 24 32 376
Fragments arranged by size:
1228 376 67 32 32 32 24
HincII GTy'rAC
Cuts at: 0 912 1791
Size: 912 879
KasI G'GCGC_C
Cuts at: 0 12 505 1095 1791
Size: 12 493 590 696
Fragments arranged by size:
696 590 493 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1703 1791
Size: 1703 88
MscI TGG'CCA
Cuts at: 0 406 1791
Size: 406 1385
MslI CAynn'nnrTG
Cuts at: 0 1012 1309 1661 1791
Size: 1012 297 352 130
Fragments arranged by size:
1012 352 297 130
MspA1I CmG'CkG
Cuts at: 0 81 204 264 324 446 474 516 600
Size: 81 123 60 60 122 28 42 84
Cuts at: 600 657 726 809 1438 1770 1791
Size: 57 69 83 629 332 21
Fragments arranged by size:
629 332 123 122 84 83 81 69
60 60 57 42 28 21
NaeI GCC'GGC
Cuts at: 0 1267 1791
Size: 1267 524
NarI GG'CG_CC
Cuts at: 0 13 506 1096 1791
Size: 13 493 590 695
Fragments arranged by size:
695 590 493 13
NcoI C'CATG_G
Cuts at: 0 1232 1304 1791
Size: 1232 72 487
Fragments arranged by size:
1232 487 72
NgoMIV G'CCGG_C
Cuts at: 0 1265 1791
Size: 1265 526
NspI r_CATG'y
Cuts at: 0 258 423 1507 1791
Size: 258 165 1084 284
Fragments arranged by size:
1084 284 258 165
PciI A'CATG_T
Cuts at: 0 1503 1791
Size: 1503 288
PflMI CCAn_nnn'nTGG
Cuts at: 0 1652 1791
Size: 1652 139
PfoI T'CCnGG_A
Cuts at: 0 101 1791
Size: 101 1690
PmlI CAC'GTG
Cuts at: 0 492 1791
Size: 492 1299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 1791
Size: 82 32 1677
Fragments arranged by size:
1677 82 32
PpuMI rG'GwC_Cy
Cuts at: 0 1773 1791
Size: 1773 18
PspOMI G'GGCC_C
Cuts at: 0 811 1791
Size: 811 980
PstI C_TGCA'G
Cuts at: 0 263 350 389 1217 1791
Size: 263 87 39 828 574
Fragments arranged by size:
828 574 263 87 39
PvuII CAG'CTG
Cuts at: 0 81 204 264 446 516 600 657 1791
Size: 81 123 60 182 70 84 57 1134
Fragments arranged by size:
1134 182 123 84 81 70 60 57
SacII CC_GC'GG
Cuts at: 0 810 1771 1791
Size: 810 961 20
Fragments arranged by size:
961 810 20
SalI G'TCGA_C
Cuts at: 0 910 1791
Size: 910 881
SanDI GG'GwC_CC
Cuts at: 0 1773 1791
Size: 1773 18
SfcI C'TryA_G
Cuts at: 0 259 346 385 981 1213 1791
Size: 259 87 39 596 232 578
Fragments arranged by size:
596 578 259 232 87 39
SfoI GGC'GCC
Cuts at: 0 14 507 1097 1791
Size: 14 493 590 694
Fragments arranged by size:
694 590 493 14
SmlI C'TyrA_G
Cuts at: 0 1068 1791
Size: 1068 723
StuI AGG'CCT
Cuts at: 0 439 1594 1791
Size: 439 1155 197
Fragments arranged by size:
1155 439 197
StyI C'CwwG_G
Cuts at: 0 578 1232 1304 1527 1791
Size: 578 654 72 223 264
Fragments arranged by size:
654 578 264 223 72
TatI w'GTAC_w
Cuts at: 0 1415 1451 1791
Size: 1415 36 340
Fragments arranged by size:
1415 340 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1284 1791
Size: 1284 507
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AhdI AlwNI ApaI
AscI AvaI BanI BanII BbeI BbvCI BciVI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EciI Eco57MI EcoO109I HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacII SalI SanDI
SfcI SfoI SmlI StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AleI AlfI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BclI BfrBI BglII BmrI BmtI BplI BsaBI
BsiWI BspEI BspHI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI ZraI