MEP1B ORF clone pFN21AE2531

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2531

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Clone info.

Species

human

Clone Name

pFN21AE2531 Sequence (with vector) Restriction Map

Product ID

FHC30764

ORF Sequence

DNA sequence (2103 bp)
Protein sequence (701 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2531

Symbol

MEP1B

Description

meprin A, beta, transcript variant 1

Original Clone ID

cp01660

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2103 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 701 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45846.1 0 100.0 MEP1B CCDS77173.1 0 99.9 MEP1B CCDS4918.1 3.6e-110 41.0 MEP1A CCDS33249.1 1e-23 37.4 ASTL CCDS7449.1 2.4e-22 30.5 TLL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001506 91 109 PR00480 Peptidase M12A IPR001506 144 162 PR00480 Peptidase M12A IPR001506 163 180 PR00480 Peptidase M12A IPR001506 202 217 PR00480 Peptidase M12A IPR001506 241 254 PR00480 Peptidase M12A IPR000998 271 289 PR00020 MAM domain IPR000998 311 327 PR00020 MAM domain IPR000998 339 350 PR00020 MAM domain IPR000998 390 404 PR00020 MAM domain IPR000998 410 423 PR00020 MAM domain HMMPfam IPR001506 70 256 PF01400 Peptidase M12A IPR000998 265 428 PF00629 MAM domain IPR002083 434 587 PF00917 MATH HMMSmart IPR006026 67 207 SM00235 Peptidase IPR000998 260 429 SM00137 MAM domain IPR002083 432 568 SM00061 MATH ProfileScan IPR000998 263 429 PS50060 MAM domain IPR002083 427 585 PS50144 MATH IPR000742 604 644 PS50026 Epidermal growth factor-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 3 LWNLSWFLFLDALLVISGLATPE 25 PRIMARY 23 2 653 IVIAVSSTVAVFALMLIITLVSV 675 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005916 701 600389 100.0 100.0 NP_001295100 700 600389 99.9 100.0 XP_011524315 628 600389 89.4 100.0 XP_011524316 577 600389 82.3 100.0 XP_011512930 774 600388 41.1 94.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2531 Download

>KIEE2531  2103 bp
ATGGATTTATGGAATCTGTCTTGGTTTCTGTTCTTGGATGCTCTTCTCGTGATTTCTGGC
TTGGCAACTCCAGAAAACTTTGATGTAGATGGCGGAATGGACCAGGACATATTTGATATC
AATGAAGGTTTGGGACTGGATCTTTTTGAGGGTGACATCAGACTTGATAGGGCACAAATT
AGAAATTCCATCATTGGAGAAAAGTATAGATGGCCTCATACCATTCCATATGTTCTAGAA
GATAGCTTGGAAATGAATGCTAAGGGAGTTATCCTCAATGCATTTGAACGTTATCGCCTT
AAAACATGTATTGACTTTAAGCCTTGGGCTGGAGAAACAAACTATATATCAGTGTTCAAG
GGCAGTGGCTGCTGGTCTTCAGTAGGAAATAGGCGGGTTGGGAAGCAAGAACTTTCCATC
GGGGCAAACTGTGACCGAATAGCAACAGTTCAACACGAGTTCCTCCACGCTCTGGGATTC
TGGCATGAGCAGTCGCGTTCTGACCGGGATGACTATGTCAGGATAATGTGGGACAGAATT
CTGTCAGGCAGAGAGCACAATTTTAACACCTATAGTGACGATATATCAGATTCCCTGAAT
GTTCCCTATGATTACACTTCAGTAATGCACTACAGTAAAACTGCATTCCAAAATGGAACA
GAGCCGACAATTGTCACAAGAATCTCAGACTTTGAGGATGTGATCGGCCAACGAATGGAT
TTCAGTGACTCTGATCTCCTAAAGTTGAATCAACTGTATAACTGCTCCTCTTCCTTGAGT
TTTATGGACTCATGCAGTTTTGAACTGGAAAATGTGTGTGGCATGATCCAAAGTTCAGGA
GATAATGCTGACTGGCAACGGGTTTCACAGGTTCCCAGGGGGCCAGAGAGTGATCACTCC
AACATGGGCCAGTGCCAAGGTTCTGGTTTCTTCATGCATTTCGATAGCAGCTCTGTAAAT
GTGGGGGCCACAGCAGTGCTGGAAAGTAGAACGCTGTACCCTAAAAGAGGATTTCAGTGC
CTGCAATTTTACTTATATAACAGTGGCAGTGAAAGTGATCAACTGAACATCTATATCAGG
GAGTATTCTGCAGACAATGTGGATGGCAATTTAACCCTTGTGGAAGAAATAAAAGAAATA
CCCACTGGGAGCTGGCAACTTTATCATGTAACATTGAAAGTGACCAAGAAGTTTAGAGTG
GTGTTTGAAGGACGCAAAGGCTCTGGTGCATCACTGGGTGGTCTGTCTATTGATGACATC
AATCTTTCGGAAACACGGTGCCCTCATCATATCTGGCATATAAGGAATTTCACACAGTTC
ATTGGCAGCCCAAATGGAACTCTGTATAGCCCTCCATTTTACTCTTCTAAAGGTTATGCC
TTTCAGATTTACTTAAATCTAGCCCATGTGACTAATGCAGGGATATATTTCCACTTGATC
TCTGGAGCCAATGATGATCAATTACAGTGGCCATGTCCTTGGCAACAAGCCACAATGACA
CTTTTGGATCAAAATCCTGACATTCGACAGCGTATGTCCAATCAGCGGAGTATAACTACA
GACCCATTTATGACCACCGATAATGGAAACTATTTCTGGGACAGGCCTTCTAAAGTGGGA
ACAGTGGCTTTGTTCTCTAATGGAACTCAGTTTAGAAGAGGTGGGGGCTATGGAACCAGT
GCCTTTATAACCCACGAAAGGCTGAAAAGCAGAGATTTTATAAAAGGAGATGATGTTTAT
ATCCTACTGACAGTGGAAGACATATCTCACCTCAACTCTACACAAATCCAGCTAACACCA
GCCCCTAGTGTTCAAGACCTCTGCTCAAAAACCACCTGTAAAAATGACGGTGTCTGCACT
GTTCGAGATGGCAAAGCTGAGTGCAGGTGCCAGTCAGGGGAAGACTGGTGGTACATGGGA
GAAAGGTGTGAAAAGAGAGGCTCCACCCGAGACACCATAGTCATTGCTGTTTCATCTACT
GTTGCTGTGTTTGCCTTGATGCTGATCATCACCCTTGTCAGTGTCTATTGCACCAGGAAG
AAATATCGTGAAAGGATGAGCTCAAATCGACCAAATTTGACTCCGCAAAATCAGCATGCT
TTT

ORF Protein

Cloned ORF protein sequence for pFN21AE2531 Download

>KIEE2531  701 aa
MDLWNLSWFLFLDALLVISGLATPENFDVDGGMDQDIFDINEGLGLDLFEGDIRLDRAQI
RNSIIGEKYRWPHTIPYVLEDSLEMNAKGVILNAFERYRLKTCIDFKPWAGETNYISVFK
GSGCWSSVGNRRVGKQELSIGANCDRIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRI
LSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQNGTEPTIVTRISDFEDVIGQRMD
FSDSDLLKLNQLYNCSSSLSFMDSCSFELENVCGMIQSSGDNADWQRVSQVPRGPESDHS
NMGQCQGSGFFMHFDSSSVNVGATAVLESRTLYPKRGFQCLQFYLYNSGSESDQLNIYIR
EYSADNVDGNLTLVEEIKEIPTGSWQLYHVTLKVTKKFRVVFEGRKGSGASLGGLSIDDI
NLSETRCPHHIWHIRNFTQFIGSPNGTLYSPPFYSSKGYAFQIYLNLAHVTNAGIYFHLI
SGANDDQLQWPCPWQQATMTLLDQNPDIRQRMSNQRSITTDPFMTTDNGNYFWDRPSKVG
TVALFSNGTQFRRGGGYGTSAFITHERLKSRDFIKGDDVYILLTVEDISHLNSTQIQLTP
APSVQDLCSKTTCKNDGVCTVRDGKAECRCQSGEDWWYMGERCEKRGSTRDTIVIAVSST
VAVFALMLIITLVSVYCTRKKYRERMSSNRPNLTPQNQHAF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2531 Download

>pFN21AE2531  6808 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATTTATGGAATCTGTCTTGGTTTCTGTTCTT
GGATGCTCTTCTCGTGATTTCTGGCTTGGCAACTCCAGAAAACTTTGATGTAGATGGCGG
AATGGACCAGGACATATTTGATATCAATGAAGGTTTGGGACTGGATCTTTTTGAGGGTGA
CATCAGACTTGATAGGGCACAAATTAGAAATTCCATCATTGGAGAAAAGTATAGATGGCC
TCATACCATTCCATATGTTCTAGAAGATAGCTTGGAAATGAATGCTAAGGGAGTTATCCT
CAATGCATTTGAACGTTATCGCCTTAAAACATGTATTGACTTTAAGCCTTGGGCTGGAGA
AACAAACTATATATCAGTGTTCAAGGGCAGTGGCTGCTGGTCTTCAGTAGGAAATAGGCG
GGTTGGGAAGCAAGAACTTTCCATCGGGGCAAACTGTGACCGAATAGCAACAGTTCAACA
CGAGTTCCTCCACGCTCTGGGATTCTGGCATGAGCAGTCGCGTTCTGACCGGGATGACTA
TGTCAGGATAATGTGGGACAGAATTCTGTCAGGCAGAGAGCACAATTTTAACACCTATAG
TGACGATATATCAGATTCCCTGAATGTTCCCTATGATTACACTTCAGTAATGCACTACAG
TAAAACTGCATTCCAAAATGGAACAGAGCCGACAATTGTCACAAGAATCTCAGACTTTGA
GGATGTGATCGGCCAACGAATGGATTTCAGTGACTCTGATCTCCTAAAGTTGAATCAACT
GTATAACTGCTCCTCTTCCTTGAGTTTTATGGACTCATGCAGTTTTGAACTGGAAAATGT
GTGTGGCATGATCCAAAGTTCAGGAGATAATGCTGACTGGCAACGGGTTTCACAGGTTCC
CAGGGGGCCAGAGAGTGATCACTCCAACATGGGCCAGTGCCAAGGTTCTGGTTTCTTCAT
GCATTTCGATAGCAGCTCTGTAAATGTGGGGGCCACAGCAGTGCTGGAAAGTAGAACGCT
GTACCCTAAAAGAGGATTTCAGTGCCTGCAATTTTACTTATATAACAGTGGCAGTGAAAG
TGATCAACTGAACATCTATATCAGGGAGTATTCTGCAGACAATGTGGATGGCAATTTAAC
CCTTGTGGAAGAAATAAAAGAAATACCCACTGGGAGCTGGCAACTTTATCATGTAACATT
GAAAGTGACCAAGAAGTTTAGAGTGGTGTTTGAAGGACGCAAAGGCTCTGGTGCATCACT
GGGTGGTCTGTCTATTGATGACATCAATCTTTCGGAAACACGGTGCCCTCATCATATCTG
GCATATAAGGAATTTCACACAGTTCATTGGCAGCCCAAATGGAACTCTGTATAGCCCTCC
ATTTTACTCTTCTAAAGGTTATGCCTTTCAGATTTACTTAAATCTAGCCCATGTGACTAA
TGCAGGGATATATTTCCACTTGATCTCTGGAGCCAATGATGATCAATTACAGTGGCCATG
TCCTTGGCAACAAGCCACAATGACACTTTTGGATCAAAATCCTGACATTCGACAGCGTAT
GTCCAATCAGCGGAGTATAACTACAGACCCATTTATGACCACCGATAATGGAAACTATTT
CTGGGACAGGCCTTCTAAAGTGGGAACAGTGGCTTTGTTCTCTAATGGAACTCAGTTTAG
AAGAGGTGGGGGCTATGGAACCAGTGCCTTTATAACCCACGAAAGGCTGAAAAGCAGAGA
TTTTATAAAAGGAGATGATGTTTATATCCTACTGACAGTGGAAGACATATCTCACCTCAA
CTCTACACAAATCCAGCTAACACCAGCCCCTAGTGTTCAAGACCTCTGCTCAAAAACCAC
CTGTAAAAATGACGGTGTCTGCACTGTTCGAGATGGCAAAGCTGAGTGCAGGTGCCAGTC
AGGGGAAGACTGGTGGTACATGGGAGAAAGGTGTGAAAAGAGAGGCTCCACCCGAGACAC
CATAGTCATTGCTGTTTCATCTACTGTTGCTGTGTTTGCCTTGATGCTGATCATCACCCT
TGTCAGTGTCTATTGCACCAGGAAGAAATATCGTGAAAGGATGAGCTCAAATCGACCAAA
TTTGACTCCGCAAAATCAGCATGCTTTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT