(Linear) (Six-base) MAPSORT of: KIEE2531.seq Check: 1965 from: 1 to: 2103 KIEE2531.orf With 182 enzymes: SgfI * July 15, 2014 16:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1874 2103 Size: 1874 229 AclI AA'CG_TT Cuts at: 0 288 2103 Size: 288 1815 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 362 602 2103 Size: 362 240 1501 Fragments arranged by size: 1501 362 240 AflIII A'CryG_T Cuts at: 0 304 2103 Size: 304 1799 AleI CACnn'nnGTG Cuts at: 0 973 2103 Size: 973 1130 AlwNI CAG_nnn'CTG Cuts at: 0 368 1020 2103 Size: 368 652 1083 Fragments arranged by size: 1083 652 368 ApoI r'AATT_y Cuts at: 0 183 536 1305 2073 2103 Size: 183 353 769 768 30 Fragments arranged by size: 769 768 353 183 30 AvaI C'yCGr_G Cuts at: 0 1946 2103 Size: 1946 157 BanI G'GyrC_C Cuts at: 0 1277 1886 2103 Size: 1277 609 217 Fragments arranged by size: 1277 609 217 BanII G_rGCy'C Cuts at: 0 2062 2103 Size: 2062 41 BbsI GAAGACnn'nnnn_ Cuts at: 0 368 1763 1907 2103 Size: 368 1395 144 196 Fragments arranged by size: 1395 368 196 144 BclI T'GATC_A Cuts at: 0 891 1056 1455 2003 2103 Size: 891 165 399 548 100 Fragments arranged by size: 891 548 399 165 100 BfrBI ATG'CAT Cuts at: 0 280 936 2103 Size: 280 656 1167 Fragments arranged by size: 1167 656 280 Bme1580I G_kGCm'C Cuts at: 0 174 1282 2103 Size: 174 1108 821 Fragments arranged by size: 1108 821 174 BmrI ACTGGGnnnn_n' Cuts at: 0 1154 1243 2103 Size: 1154 89 860 Fragments arranged by size: 1154 860 89 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 289 2103 Size: 289 1814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 350 1463 2103 Size: 53 297 1113 640 Fragments arranged by size: 1113 640 297 53 Bpu10I CC'TnA_GC Cuts at: 0 260 2103 Size: 260 1843 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 795 2103 Size: 795 1308 BsaBI GATnn'nnATC Cuts at: 0 1511 2002 2103 Size: 1511 491 101 Fragments arranged by size: 1511 491 101 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1718 1748 2103 Size: 1718 30 355 Fragments arranged by size: 1718 355 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 756 2103 Size: 756 1347 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1839 1902 2103 Size: 1839 63 201 Fragments arranged by size: 1839 201 63 BsiHKAI G_wGCw'C Cuts at: 0 557 2062 2103 Size: 557 1505 41 Fragments arranged by size: 1505 557 41 BsmI GAATG_Cn' Cuts at: 0 261 643 2103 Size: 261 382 1460 Fragments arranged by size: 1460 382 261 Bsp1286I G_dGCh'C Cuts at: 0 174 557 1282 2062 2103 Size: 174 383 725 780 41 Fragments arranged by size: 780 725 383 174 41 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1874 2103 Size: 1874 229 BsrDI GCAATG_nn' Cuts at: 0 1961 2103 Size: 1961 142 BssSI C'ACGA_G Cuts at: 0 46 454 2103 Size: 46 408 1649 Fragments arranged by size: 1649 408 46 BstAPI GCAn_nnn'nTGC Cuts at: 0 368 2103 Size: 368 1735 BstXI CCAn_nnnn'nTGG Cuts at: 0 922 1478 2103 Size: 922 556 625 Fragments arranged by size: 922 625 556 BstYI r'GATC_y Cuts at: 0 138 2103 Size: 138 1965 BtsI GCAGTG_nn' Cuts at: 0 369 980 1053 2103 Size: 369 611 73 1050 Fragments arranged by size: 1050 611 369 73 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 345 380 1447 1482 2103 Size: 345 35 1067 35 621 Fragments arranged by size: 1067 621 345 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1237 2103 Size: 1237 866 DrdI GACnn_nn'nnGTC Cuts at: 0 1957 2103 Size: 1957 146 EaeI y'GGCC_r Cuts at: 0 705 1468 2103 Size: 705 763 635 Fragments arranged by size: 763 705 635 EarI CTCTTCn'nnn_ Cuts at: 0 47 774 1368 1650 2103 Size: 47 727 594 282 453 Fragments arranged by size: 727 594 453 282 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 107 2103 Size: 107 1996 EcoICRI GAG'CTC Cuts at: 0 2060 2103 Size: 2060 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 350 362 602 1463 2103 Size: 53 297 12 240 861 640 Fragments arranged by size: 861 640 297 240 53 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1004 2103 Size: 1004 1099 EcoRI G'AATT_C Cuts at: 0 536 2103 Size: 536 1567 EcoRV GAT'ATC Cuts at: 0 117 2103 Size: 117 1986 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 568 600 934 966 1807 1839 2049 2081 Size: 568 32 334 32 841 32 210 32 Cuts at: 2081 2103 Size: 22 Fragments arranged by size: 841 568 334 210 32 32 32 32 22 MfeI C'AATT_G Cuts at: 0 668 2103 Size: 668 1435 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 923 2103 Size: 923 1180 MscI TGG'CCA Cuts at: 0 1470 2103 Size: 1470 633 MslI CAynn'nnrTG Cuts at: 0 973 2103 Size: 973 1130 MspA1I CmG'CkG Cuts at: 0 1545 2103 Size: 1545 558 NdeI CA'TA_TG Cuts at: 0 228 2103 Size: 228 1875 NsiI A_TGCA'T Cuts at: 0 282 938 2103 Size: 282 656 1165 Fragments arranged by size: 1165 656 282 NspI r_CATG'y Cuts at: 0 308 2098 2103 Size: 308 1790 5 Fragments arranged by size: 1790 308 5 PasI CC'CwG_GG Cuts at: 0 875 2103 Size: 875 1228 PciI A'CATG_T Cuts at: 0 304 2103 Size: 304 1799 PflMI CCAn_nnn'nTGG Cuts at: 0 471 714 2103 Size: 471 243 1389 Fragments arranged by size: 1389 471 243 PshAI GACnn'nnGTC Cuts at: 0 670 2103 Size: 670 1433 PsiI TTA'TAA Cuts at: 0 1687 1720 2103 Size: 1687 33 383 Fragments arranged by size: 1687 383 33 PstI C_TGCA'G Cuts at: 0 1092 2103 Size: 1092 1011 SacI G_AGCT'C Cuts at: 0 2062 2103 Size: 2062 41 SapI GCTCTTCn'nnn_ Cuts at: 0 47 2103 Size: 47 2056 SfcI C'TryA_G Cuts at: 0 570 630 1088 1556 2103 Size: 570 60 458 468 547 Fragments arranged by size: 570 547 468 458 60 SmlI C'TyrA_G Cuts at: 0 774 2103 Size: 774 1329 SphI G_CATG'C Cuts at: 0 2098 2103 Size: 2098 5 StuI AGG'CCT Cuts at: 0 1605 2103 Size: 1605 498 StyI C'CwwG_G Cuts at: 0 322 915 1477 2103 Size: 322 593 562 626 Fragments arranged by size: 626 593 562 322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 449 1225 2103 Size: 449 776 878 Fragments arranged by size: 878 776 449 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 799 831 1261 1293 1891 1923 2103 Size: 799 32 430 32 598 32 180 Fragments arranged by size: 799 598 430 180 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 514 1849 2103 Size: 514 1335 254 Fragments arranged by size: 1335 514 254 XbaI T'CTAG_A Cuts at: 0 234 2103 Size: 234 1869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 975 1438 2103 Size: 975 463 665 Fragments arranged by size: 975 665 463 Enzymes that do cut: AarI AclI AcuI AflIII AleI AlwNI ApoI AvaI BanI BanII BbsI BclI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV Hin4I MfeI MmeI MscI MslI MspA1I NdeI NsiI NspI PasI PciI PflMI PshAI PsiI PstI SacI SapI SfcI SmlI SphI StuI StyI TaqII TstI Tth111I XbaI XcmI Enzymes that do not cut: AatII AccI Acc65I AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmtI BsaI BsaAI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraI EagI EcoO109I FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI XhoI XmaI XmnI ZraI