################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 494 CGCG CG^CG AclI 287 AACGTT AA^CGTT AflIII 304 ACATGT A^C[GA][CT]GT AvaI 1946 CCCGAG C^[CT]CG[GA]G AvaII 99 GGACC G^G[AT]CC BalI 1468 TGGCCA TGG^CCA BanII 2058 GAGCTC G[GA]GC[CT]^C BcnI 504 CCGGG CC^[GC]GG BseRI 765 CTCCTC GAGGAG(10/8) BsmAI 1949 GAGAC GTCTC(1/5) BsoBI 1946 CCCGAG C^[CT]CG[GA]G BspLU11I 304 ACATGT A^CATGT BspMI 1883 GCAGGT ACCTGC(4/8) BsrDI 1962 CATTGC GCAATG(2/0) BstAPI 362 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 494 CGCG CG^CG BstYI 138 GGATCT [GA]^GATC[CT] DraIII 1232 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1951 GACACCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1000 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 536 GAATTC G^AATTC EcoRV 115 GATATC GAT^ATC FauI 393 GCGGG CCCGC(4/6) HapII 504 CCGG C^CGG HgaI 1211 GACGC GACGC(5/10) HpaII 504 CCGG C^CGG MaeII 288 ACGT A^CGT MfeI 668 CAATTG C^AATTG MflI 138 GGATCT [GA]^GATC[CT] MluNI 1468 TGGCCA TGG^CCA MscI 1468 TGGCCA TGG^CCA MslI 969 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1543 CAGCGG C[AC]G^C[GT]G MspI 504 CCGG C^CGG MunI 668 CAATTG C^AATTG MvnI 494 CGCG CG^CG NciI 504 CCGGG CC^[GC]GG NdeI 227 CATATG CA^TATG PshAI 666 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 287 AACGTT AA^CGTT PstI 1088 CTGCAG CTGCA^G SacI 2058 GAGCTC GAGCT^C SapI 40 GCTCTTC GCTCTTC(1/4) SmlI 774 CTTGAG C^T[CT][GA]AG SphI 2094 GCATGC GCATG^C StuI 1603 AGGCCT AGG^CCT XbaI 234 TCTAGA T^CTAGA XhoII 138 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 996 GTAC GT^AC 1911 GTAC AlwNI 363 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1015 CAGTGCCTG AspHI 553 GAGCAC G[AT]GC[AT]^C 2058 GAGCTC AspI 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1846 GACGGTGTC BanI 1277 GGTGCC G^G[CT][GA]CC 1886 GGTGCC BsaBI 1507 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1998 GATGCTGATC BsgI 1854 CTGCAC GTGCAG(16/14) 1881 GTGCAG BsiHKAI 553 GAGCAC G[AT]GC[AT]^C 2058 GAGCTC BsmI 255 GAATGC GAATGC(1/-1) 643 GCATTC BssSI 454 CACGAG CACGAG(-5/-1) 46 CTCGTG BstXI 915 CCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1471 CCATGTCCTTGG EaeI 705 CGGCCA [CT]^GGCC[GA] 1468 TGGCCA EcoT22I 278 ATGCAT ATGCA^T 934 ATGCAT MamI 1507 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1998 GATGCTGATC NsiI 278 ATGCAT ATGCA^T 934 ATGCAT NspI 304 ACATGT [GA]CATG^[CT] 2094 GCATGC PflFI 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1846 GACGGTGTC PflMI 465 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 708 CCAACGAATGG RsaI 996 GTAC GT^AC 1911 GTAC Tth111I 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1846 GACGGTGTC Van91I 465 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 708 CCAACGAATGG XcmI 968 CCACAGCAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1431 CCACTTGATCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I