(Circular) (Six-base) MAPSORT of: pFN21AE2531.seq Check: 3127 from: 1 to: 6808 pFN21AE2531 6808 bp With 182 enzymes: SgfI * July 15, 2014 17:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3879 3879 Size: 6808 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4618 278 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4151 1937 Size: 2214 4594 Acc65I G'GTAC_C Cuts at: 4129 4129 Size: 6808 AclI AA'CG_TT Cuts at: 1563 2293 4935 5308 1563 Size: 730 2642 373 3063 Fragments arranged by size: 3063 2642 730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2367 2607 4871 6255 6523 1632 Size: 735 240 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 735 268 240 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6611 AflIII A'CryG_T Cuts at: 1203 2309 5707 6684 1203 Size: 1106 3398 977 1327 Fragments arranged by size: 3398 1327 1106 977 AgeI A'CCGG_T Cuts at: 5639 5639 Size: 6808 AhdI GACnn_n'nnGTC Cuts at: 5537 5537 Size: 6808 AleI CACnn'nnGTG Cuts at: 1166 2978 1166 Size: 1812 4996 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6808 AlwNI CAG_nnn'CTG Cuts at: 2373 3025 4551 6123 2373 Size: 652 1526 1572 3058 Fragments arranged by size: 3058 1572 1526 652 ApaLI G'TGCA_C Cuts at: 4865 6021 4865 Size: 1156 5652 ApoI r'AATT_y Cuts at: 1493 2188 2541 3310 4078 4117 4343 4495 6604 Size: 695 353 769 768 39 226 152 2109 Cuts at: 6604 1493 Size: 1697 Fragments arranged by size: 2109 1697 769 768 695 353 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 5362 160 Size: 872 4330 1606 Fragments arranged by size: 4330 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 AvaI C'yCGr_G Cuts at: 1870 1943 3951 4133 1870 Size: 73 2008 182 4545 Fragments arranged by size: 4545 2008 182 73 BamHI G'GATC_C Cuts at: 4138 4505 4138 Size: 367 6441 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3282 3891 4129 5584 618 Size: 325 217 627 1495 609 238 1455 1842 Fragments arranged by size: 1842 1495 1455 627 609 325 238 217 BanII G_rGCy'C Cuts at: 1391 4067 4127 1391 Size: 2676 60 4072 Fragments arranged by size: 4072 2676 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2373 3768 3912 928 Size: 971 474 1395 144 3824 Fragments arranged by size: 3824 1395 971 474 144 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4999 5033 1903 Size: 34 3062 34 3678 Fragments arranged by size: 3678 3062 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4700 5910 4700 Size: 1210 5598 BclI T'GATC_A Cuts at: 1548 2896 3061 3460 4008 1548 Size: 1348 165 399 548 4348 Fragments arranged by size: 4348 1348 548 399 165 BfrBI ATG'CAT Cuts at: 2285 2941 2285 Size: 656 6152 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5419 136 Size: 107 122 71 631 605 3747 1525 Fragments arranged by size: 3747 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6803 6803 Size: 6808 BlpI GC'TnA_GC Cuts at: 4225 4225 Size: 6808 Bme1580I G_kGCm'C Cuts at: 1790 2179 3287 4869 6025 1790 Size: 389 1108 1582 1156 2573 Fragments arranged by size: 2573 1582 1156 1108 389 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6808 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3159 3248 4539 Size: 416 53 83 399 24 1742 89 1291 Cuts at: 4539 5497 442 Size: 958 1753 Fragments arranged by size: 1753 1742 1291 958 416 399 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6808 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2294 2294 Size: 6808 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 2355 3468 5468 1239 Size: 819 297 1113 2000 2579 Fragments arranged by size: 2579 2000 1113 819 297 Bpu10I CC'TnA_GC Cuts at: 2265 2265 Size: 6808 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2800 4933 5798 6096 2800 Size: 2133 865 298 3512 Fragments arranged by size: 3512 2133 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5471 882 Size: 617 3972 2219 Fragments arranged by size: 3972 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4599 493 Size: 711 3395 2702 Fragments arranged by size: 3395 2702 711 BsaBI GATnn'nnATC Cuts at: 3516 4007 6802 3516 Size: 491 2795 3522 Fragments arranged by size: 3522 2795 491 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4615 4997 5632 275 Size: 53 83 186 936 3082 382 635 1451 Fragments arranged by size: 3082 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5241 5639 5913 6060 6471 5241 Size: 398 274 147 411 5578 Fragments arranged by size: 5578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3723 3753 1178 Size: 30 165 30 2320 30 4233 Fragments arranged by size: 4233 2320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2761 1186 Size: 1575 5233 BseYI C'CCAG_C Cuts at: 6011 6507 6011 Size: 496 6312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3844 3907 1163 Size: 573 2108 63 4064 Fragments arranged by size: 4064 2108 573 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4258 5019 6047 1540 Size: 275 186 2257 761 1028 2301 Fragments arranged by size: 2301 2257 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2562 4067 4869 4954 6025 2562 Size: 1505 802 85 1071 3345 Fragments arranged by size: 3345 1505 1071 802 85 BsmI GAATG_Cn' Cuts at: 2266 2648 4319 4412 2266 Size: 382 1671 93 4662 Fragments arranged by size: 4662 1671 382 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2562 3287 4067 4127 4869 4954 Size: 399 389 383 725 780 60 742 85 Cuts at: 4954 6025 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 780 742 725 399 389 383 85 60 BspEI T'CCGG_A Cuts at: 6471 6471 Size: 6808 BspHI T'CATG_A Cuts at: 4697 6427 4697 Size: 1730 5078 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3879 4163 844 Size: 3035 284 3489 Fragments arranged by size: 3489 3035 284 BsrBI CCG'CTC Cuts at: 1394 1823 4695 1394 Size: 429 2872 3507 Fragments arranged by size: 3507 2872 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3966 5303 5477 65 Size: 1183 2718 1337 174 1396 Fragments arranged by size: 2718 1396 1337 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5452 5639 1700 Size: 110 3642 187 2869 Fragments arranged by size: 3642 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6808 BssSI C'ACGA_G Cuts at: 2051 2459 4868 5880 2051 Size: 408 2409 1012 2979 Fragments arranged by size: 2979 2409 1012 408 BstAPI GCAn_nnn'nTGC Cuts at: 794 2373 794 Size: 1579 5229 BstBI TT'CG_AA Cuts at: 5612 5612 Size: 6808 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6808 BstXI CCAn_nnnn'nTGG Cuts at: 2927 3483 2927 Size: 556 6252 BstYI r'GATC_y Cuts at: 1972 2143 4138 4505 4890 4907 6359 6445 6457 Size: 171 1995 367 385 17 1452 86 12 Cuts at: 6457 6803 1972 Size: 346 1977 Fragments arranged by size: 1995 1977 1452 385 367 346 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5317 Fragments arranged by size: 5317 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6069 Fragments arranged by size: 6069 683 56 BtsI GCAGTG_nn' Cuts at: 2374 2985 3058 4324 5118 5138 2374 Size: 611 73 1266 794 20 4044 Fragments arranged by size: 4044 1266 794 611 73 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6808 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2350 2385 3452 3487 529 Size: 35 1786 35 1067 35 3850 Fragments arranged by size: 3850 1786 1067 35 35 35 DraI TTT'AAA Cuts at: 4112 4112 Size: 6808 DraIII CAC_nnn'GTG Cuts at: 3242 3242 Size: 6808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3962 5815 809 Size: 507 2646 1853 1802 Fragments arranged by size: 2646 1853 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2710 3473 4255 Size: 54 1425 50 275 18 880 763 782 Cuts at: 4255 5144 6773 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 880 782 763 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4255 1537 Size: 275 2443 4090 Fragments arranged by size: 4090 2443 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2779 3373 3655 4738 1353 Size: 699 727 594 282 1083 3423 Fragments arranged by size: 3423 1083 727 699 594 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2112 5395 5769 5915 387 Size: 1064 138 523 3283 374 146 1280 Fragments arranged by size: 3283 1280 1064 523 374 146 138 EcoICRI GAG'CTC Cuts at: 4065 4065 Size: 6808 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2355 2367 2607 3468 4871 5468 Size: 393 426 297 12 240 861 1403 597 Cuts at: 5468 6255 6523 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1403 861 787 597 426 393 297 268 240 12 EcoNI CCTnn'n_nnAGG Cuts at: 3009 3009 Size: 6808 EcoRI G'AATT_C Cuts at: 2541 4117 4495 2541 Size: 1576 378 4854 Fragments arranged by size: 4854 1576 378 EcoRV GAT'ATC Cuts at: 2122 2122 Size: 6808 FspI TGC'GCA Cuts at: 5314 5314 Size: 6808 HaeII r_GCGC'y Cuts at: 1678 5955 1678 Size: 4277 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2573 2605 2939 2971 3812 Size: 32 135 32 1001 32 334 32 841 Cuts at: 3812 3844 4054 4086 5449 5481 5523 5555 1373 Size: 32 210 32 1363 32 42 32 2626 Fragments arranged by size: 2626 1363 1001 841 334 210 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4152 4398 669 Size: 988 281 2214 246 3079 Fragments arranged by size: 3079 2214 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6808 HpaI GTT'AAC Cuts at: 4398 4398 Size: 6808 KpnI G_GTAC'C Cuts at: 4133 4133 Size: 6808 MfeI C'AATT_G Cuts at: 2673 4407 2673 Size: 1734 5074 MluI A'CGCG_T Cuts at: 6684 6684 Size: 6808 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2928 5922 6106 1305 Size: 1623 2994 184 2007 Fragments arranged by size: 2994 2007 1623 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3475 10 Size: 54 1425 343 1643 3343 Fragments arranged by size: 3343 1643 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2978 4766 5125 5284 518 Size: 648 1812 1788 359 159 2042 Fragments arranged by size: 2042 1812 1788 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3550 4224 4901 6049 6294 1606 Size: 1944 674 677 1148 245 2120 Fragments arranged by size: 2120 1944 1148 677 674 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5317 Fragments arranged by size: 5317 939 552 NdeI CA'TA_TG Cuts at: 387 2233 387 Size: 1846 4962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6808 NotI GC'GGCC_GC Cuts at: 4255 4255 Size: 6808 NsiI A_TGCA'T Cuts at: 2287 2943 2287 Size: 656 6152 NspI r_CATG'y Cuts at: 1132 1229 2313 4103 4166 5711 1132 Size: 97 1084 1790 63 1545 2229 Fragments arranged by size: 2229 1790 1545 1084 97 63 PasI CC'CwG_GG Cuts at: 1348 2880 1348 Size: 1532 5276 PciI A'CATG_T Cuts at: 2309 5707 2309 Size: 3398 3410 PflMI CCAn_nnn'nTGG Cuts at: 1108 2476 2719 1108 Size: 1368 243 5197 Fragments arranged by size: 5197 1368 243 PmeI GTTT'AAAC Cuts at: 4112 4112 Size: 6808 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4877 4909 6390 6422 4877 Size: 32 1481 32 5263 Fragments arranged by size: 5263 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2675 1613 Size: 1062 5746 PsiI TTA'TAA Cuts at: 3692 3725 4378 3692 Size: 33 653 6122 Fragments arranged by size: 6122 653 33 PstI C_TGCA'G Cuts at: 830 3097 4160 830 Size: 2267 1063 3478 Fragments arranged by size: 3478 2267 1063 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6808 SacI G_AGCT'C Cuts at: 4067 4067 Size: 6808 SalI G'TCGA_C Cuts at: 1936 4150 1936 Size: 2214 4594 SapI GCTCTTCn'nnn_ Cuts at: 2052 2052 Size: 6808 SbfI CC_TGCA'GG Cuts at: 4160 4160 Size: 6808 ScaI AGT'ACT Cuts at: 5056 6768 5056 Size: 1712 5096 SfcI C'TryA_G Cuts at: 826 1045 2575 2635 3093 3561 4156 5291 5972 Size: 219 1530 60 458 468 595 1135 681 Cuts at: 5972 6163 826 Size: 191 1471 Fragments arranged by size: 1530 1471 1135 681 595 468 458 219 191 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6808 SmaI CCC'GGG Cuts at: 1872 4135 1872 Size: 2263 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2779 4912 5813 6075 820 Size: 197 926 836 2133 901 262 1553 Fragments arranged by size: 2133 1553 926 901 836 262 197 SnaBI TAC'GTA Cuts at: 493 4599 493 Size: 4106 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6808 SphI G_CATG'C Cuts at: 1132 4103 4166 1132 Size: 2971 63 3774 Fragments arranged by size: 3774 2971 63 SspI AAT'ATT Cuts at: 5 52 4732 5 Size: 47 4680 2081 Fragments arranged by size: 4680 2081 47 StuI AGG'CCT Cuts at: 1517 3610 1517 Size: 2093 4715 StyI C'CwwG_G Cuts at: 513 1065 2004 2327 2920 3482 4247 513 Size: 552 939 323 593 562 765 3074 Fragments arranged by size: 3074 939 765 593 562 552 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2454 3230 4835 4852 5005 5190 5635 Size: 1255 874 776 1605 17 153 185 445 Cuts at: 5635 325 Size: 1498 Fragments arranged by size: 1605 1498 1255 874 776 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5054 6766 96 Size: 274 80 33 51 1443 3077 1712 138 Fragments arranged by size: 3077 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2804 2836 3266 3298 3896 3928 6768 6800 2804 Size: 32 430 32 598 32 2840 32 2812 Fragments arranged by size: 2840 2812 598 430 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2519 3854 1316 Size: 1203 1335 4270 Fragments arranged by size: 4270 1335 1203 XbaI T'CTAG_A Cuts at: 2239 4144 2239 Size: 1905 4903 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 3443 2980 Size: 463 6345 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6808 XmaI C'CCGG_G Cuts at: 1870 4133 1870 Size: 2263 4545 XmnI GAAnn'nnTTC Cuts at: 4937 4937 Size: 6808 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4616 276 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI