RTN2 ORF clone pFN21AE2481

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Halotag ORF Clone pFN21AE2481

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Clone info.

Species

human

Clone Name

pFN21AE2481 Sequence (with vector) Restriction Map

Product ID

FHC26693

ORF Sequence

DNA sequence (1416 bp)
Protein sequence (472 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2481

Symbol

RTN2

Alias : NSP2, NSPL1, NSPLI, SPG12

Description

reticulon 2, transcript variant 2

Original Clone ID

cp01624

Note

A star activity of SgfI may be observed.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1416 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 472 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12666.1 1.4e-120 100.0 RTN2 CCDS12665.1 9.4e-70 85.5 RTN2 CCDS46114.1 1.3e-45 100.0 RTN2 CCDS9740.1 1.4e-22 36.6 RTN1 CCDS42683.1 4.7e-22 35.5 RTN4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003388 272 441 PF02453 Reticulon ProfileScan IPR003388 272 472 PS50845 Reticulon
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 291 LMVSLLCLLHFSIVSVAAHLALL 313 PRIMARY 23 2 376 FFLVEDLVDSLKLALLFYILTFV 398 PRIMARY 23 3 401 IFNGLTLLILGVIGLFTIPLLY 422 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_996783 472 603183,604805 100.0 100.0 NP_005610 545 603183,604805 85.5 91.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2481 Download

>KIEE2481  1416 bp
ATGGGGCAGGTCCTGCCGGTCTTCGCCCACTGCAAAGAAGCTCCGTCTACAGCCTCCTCA
ACTCCTGATTCCACAGAAGGAGGGAACGACGACTCTGATTTTCGAGAGCTGCACACAGCC
CGGGAATTCTCAGAGGAGGACGAGGAGGAGACCACGTCGCAGGACTGGGGCACCCCCCGG
GAGCTGACCTTCTCCTACATCGCCTTTGATGGTGTAGTGGGCTCCGGGGGCCGCAGGGAT
TCAACTGCCCGCCGCCCCCGCCCCCAGGGCCGCTCAGTCTCGGAACCACGAGACCAGCAC
CCTCAGCCCAGCCTGGGCGACAGCTTGGAGAGCATCCCCAGCCTGAGCCAATCCCCGGAG
CCTGGACGACGGGGTGATCCTGACACCGCGCCTCCATCCGAGCGCCCTCTGGAAGACCTG
AGGCTTCGGTTGGACCATCTGGGCTGGGTGGCCCGGGGAACGGGATCCGGGGAGGACTCT
TCCACCAGCAGCTCCACCCCGCTGGAAGACGAAGAACCCCAAGAACCCAACAGATTGGAG
ACAGGAGAAGCTGGGGAAGAACTGGACCTACGACTCCGACTTGCTCAGCCCTCATCGCCC
GAGGTCTTGACTCCCCAGCTCAGTCCGGGCTCTGGGACACCCCAGGCCGGTACTCCGTCC
CCATCCCGATCGCGAGATTCGAACTCTGGGCCCGAAGAGCCATTGCTGGAAGAGGAAGAA
AAGCAGTGGGGGCCACTGGAGCGAGAGCCAGTAAGGGGACAGTGCCTCGATAGCACGGAC
CAATTAGAATTCACGGTGGAGCCACGCCTTCTAGTGGCGGACCTGCTGTACTGGAAGGAC
ACGAGGACGTCAGGAGTGGTCTTCACAGGCCTGATGGTCTCCCTCCTCTGCCTCCTGCAC
TTTAGCATCGTGTCCGTGGCCGCGCACTTGGCTCTGTTGCTGCTCTGCGGCACCATCTCT
CTCAGGGTTTACCGCAAAGTGCTGCAGGCCGTGCACCGGGGGGATGGAGCCAACCCTTTC
CAGGCCTACCTGGATGTGGACCTCACCCTGACTCGGGAGCAGACGGAACGTTTGTCCCAC
CAGATCACCTCCCGCGTGGTCTCGGCGGCCACGCAGCTGCGGCACTTCTTCCTGGTAGAA
GACCTCGTGGATTCCCTCAAGCTGGCCCTCCTCTTCTACATCTTGACCTTCGTGGGTGCC
ATCTTCAATGGTTTGACTCTTCTCATTCTGGGAGTGATTGGTCTATTCACCATCCCCCTG
CTGTACCGGCAGCACCAGGCTCAGATCGACCAATATGTGGGGTTGGTGACCAATCAGTTG
AGCCACATCAAAGCTAAGATCCGAGCTAAAATCCCAGGGACCGGAGCCCTGGCCTCTGCA
GCAGCCGCAGTCTCCGGATCCAAAGCCAAAGCCGAA

ORF Protein

Cloned ORF protein sequence for pFN21AE2481 Download

>KIEE2481  472 aa
MGQVLPVFAHCKEAPSTASSTPDSTEGGNDDSDFRELHTAREFSEEDEEETTSQDWGTPR
ELTFSYIAFDGVVGSGGRRDSTARRPRPQGRSVSEPRDQHPQPSLGDSLESIPSLSQSPE
PGRRGDPDTAPPSERPLEDLRLRLDHLGWVARGTGSGEDSSTSSSTPLEDEEPQEPNRLE
TGEAGEELDLRLRLAQPSSPEVLTPQLSPGSGTPQAGTPSPSRSRDSNSGPEEPLLEEEE
KQWGPLEREPVRGQCLDSTDQLEFTVEPRLLVADLLYWKDTRTSGVVFTGLMVSLLCLLH
FSIVSVAAHLALLLLCGTISLRVYRKVLQAVHRGDGANPFQAYLDVDLTLTREQTERLSH
QITSRVVSAATQLRHFFLVEDLVDSLKLALLFYILTFVGAIFNGLTLLILGVIGLFTIPL
LYRQHQAQIDQYVGLVTNQLSHIKAKIRAKIPGTGALASAAAAVSGSKAKAE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2481 Download

>pFN21AE2481  6121 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCAGGTCCTGCCGGTCTTCGCCCACTGCAA
AGAAGCTCCGTCTACAGCCTCCTCAACTCCTGATTCCACAGAAGGAGGGAACGACGACTC
TGATTTTCGAGAGCTGCACACAGCCCGGGAATTCTCAGAGGAGGACGAGGAGGAGACCAC
GTCGCAGGACTGGGGCACCCCCCGGGAGCTGACCTTCTCCTACATCGCCTTTGATGGTGT
AGTGGGCTCCGGGGGCCGCAGGGATTCAACTGCCCGCCGCCCCCGCCCCCAGGGCCGCTC
AGTCTCGGAACCACGAGACCAGCACCCTCAGCCCAGCCTGGGCGACAGCTTGGAGAGCAT
CCCCAGCCTGAGCCAATCCCCGGAGCCTGGACGACGGGGTGATCCTGACACCGCGCCTCC
ATCCGAGCGCCCTCTGGAAGACCTGAGGCTTCGGTTGGACCATCTGGGCTGGGTGGCCCG
GGGAACGGGATCCGGGGAGGACTCTTCCACCAGCAGCTCCACCCCGCTGGAAGACGAAGA
ACCCCAAGAACCCAACAGATTGGAGACAGGAGAAGCTGGGGAAGAACTGGACCTACGACT
CCGACTTGCTCAGCCCTCATCGCCCGAGGTCTTGACTCCCCAGCTCAGTCCGGGCTCTGG
GACACCCCAGGCCGGTACTCCGTCCCCATCCCGATCGCGAGATTCGAACTCTGGGCCCGA
AGAGCCATTGCTGGAAGAGGAAGAAAAGCAGTGGGGGCCACTGGAGCGAGAGCCAGTAAG
GGGACAGTGCCTCGATAGCACGGACCAATTAGAATTCACGGTGGAGCCACGCCTTCTAGT
GGCGGACCTGCTGTACTGGAAGGACACGAGGACGTCAGGAGTGGTCTTCACAGGCCTGAT
GGTCTCCCTCCTCTGCCTCCTGCACTTTAGCATCGTGTCCGTGGCCGCGCACTTGGCTCT
GTTGCTGCTCTGCGGCACCATCTCTCTCAGGGTTTACCGCAAAGTGCTGCAGGCCGTGCA
CCGGGGGGATGGAGCCAACCCTTTCCAGGCCTACCTGGATGTGGACCTCACCCTGACTCG
GGAGCAGACGGAACGTTTGTCCCACCAGATCACCTCCCGCGTGGTCTCGGCGGCCACGCA
GCTGCGGCACTTCTTCCTGGTAGAAGACCTCGTGGATTCCCTCAAGCTGGCCCTCCTCTT
CTACATCTTGACCTTCGTGGGTGCCATCTTCAATGGTTTGACTCTTCTCATTCTGGGAGT
GATTGGTCTATTCACCATCCCCCTGCTGTACCGGCAGCACCAGGCTCAGATCGACCAATA
TGTGGGGTTGGTGACCAATCAGTTGAGCCACATCAAAGCTAAGATCCGAGCTAAAATCCC
AGGGACCGGAGCCCTGGCCTCTGCAGCAGCCGCAGTCTCCGGATCCAAAGCCAAAGCCGA
AGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
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