(Linear) (Six-base) MAPSORT of: KIEE2481.seq Check: 8422 from: 1 to: 1416 KIEE2481.orf With 182 enzymes: SgfI * August 14, 2013 11:53 .. AatII G_ACGT'C Cuts at: 0 850 1416 Size: 850 566 AccI GT'mk_AC Cuts at: 0 46 1416 Size: 46 1370 AclI AA'CG_TT Cuts at: 0 1068 1416 Size: 1068 348 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 997 1416 Size: 997 419 AlwNI CAG_nnn'CTG Cuts at: 0 12 1416 Size: 12 1404 ApaI G_GGCC'C Cuts at: 0 692 1416 Size: 692 724 ApaLI G'TGCA_C Cuts at: 0 991 1416 Size: 991 425 ApoI r'AATT_y Cuts at: 0 124 787 1416 Size: 124 663 629 Fragments arranged by size: 663 629 124 AvaI C'yCGr_G Cuts at: 0 119 176 452 598 1052 1416 Size: 119 57 276 146 454 364 Fragments arranged by size: 454 364 276 146 119 57 BamHI G'GATC_C Cuts at: 0 463 1396 1416 Size: 463 933 20 Fragments arranged by size: 933 463 20 BanI G'GyrC_C Cuts at: 0 169 949 1195 1416 Size: 169 780 246 221 Fragments arranged by size: 780 246 221 169 BanII G_rGCy'C Cuts at: 0 223 631 692 1368 1416 Size: 223 408 61 676 48 Fragments arranged by size: 676 408 223 61 48 BbsI GAAGACnn'nnnn_ Cuts at: 0 12 419 512 852 1145 1416 Size: 12 407 93 340 293 271 Fragments arranged by size: 407 340 293 271 93 12 BbvCI CC'TCA_GC Cuts at: 0 302 1416 Size: 302 1114 BglI GCCn_nnn'nGGC Cuts at: 0 266 1416 Size: 266 1150 BlpI GC'TnA_GC Cuts at: 0 584 1416 Size: 584 832 Bme1580I G_kGCm'C Cuts at: 0 172 692 995 1416 Size: 172 520 303 421 Fragments arranged by size: 520 421 303 172 BmgBI CAC'GTC Cuts at: 0 155 1416 Size: 155 1261 BmrI ACTGGGnnnn_n' Cuts at: 0 174 1416 Size: 174 1242 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 1416 Size: 757 659 Bpu10I CC'TnA_GC Cuts at: 0 302 343 1416 Size: 302 41 1073 Fragments arranged by size: 1073 302 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1141 1416 Size: 1141 275 BsaI GGTCTCn'nnnn_ Cuts at: 0 142 284 882 1104 1416 Size: 142 142 598 222 312 Fragments arranged by size: 598 312 222 142 142 BsaHI Gr'CG_yC Cuts at: 0 847 1416 Size: 847 569 BsaWI w'CCGG_w Cuts at: 0 1360 1393 1416 Size: 1360 33 23 Fragments arranged by size: 1360 33 23 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 139 169 375 405 1416 Size: 139 30 206 30 1011 Fragments arranged by size: 1011 206 139 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 45 148 157 160 874 1159 1416 Size: 45 103 9 3 714 285 257 Fragments arranged by size: 714 285 257 103 45 9 3 BseYI C'CCAG_C Cuts at: 0 307 337 443 550 614 1416 Size: 307 30 106 107 64 802 Fragments arranged by size: 802 307 107 106 64 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 880 1416 Size: 94 786 536 Fragments arranged by size: 786 536 94 BsiEI CG_ry'CG Cuts at: 0 670 1416 Size: 670 746 BsiHKAI G_wGCw'C Cuts at: 0 995 1416 Size: 995 421 Bsp1286I G_dGCh'C Cuts at: 0 172 223 631 692 995 1368 1416 Size: 172 51 408 61 303 373 48 Fragments arranged by size: 408 373 303 172 61 51 48 BspEI T'CCGG_A Cuts at: 0 1393 1416 Size: 1393 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 830 1416 Size: 830 586 BsrBI CCG'CTC Cuts at: 0 272 1416 Size: 272 1144 BsrDI GCAATG_nn' Cuts at: 0 700 1416 Size: 700 716 BsrFI r'CCGG_y Cuts at: 0 15 646 1265 1416 Size: 15 631 619 151 Fragments arranged by size: 631 619 151 15 BssSI C'ACGA_G Cuts at: 0 287 840 1144 1416 Size: 287 553 304 272 Fragments arranged by size: 553 304 287 272 BstAPI GCAn_nnn'nTGC Cuts at: 0 12 990 1416 Size: 12 978 426 Fragments arranged by size: 978 426 12 BstBI TT'CG_AA Cuts at: 0 679 1416 Size: 679 737 BstEII G'GTnAC_C Cuts at: 0 1305 1416 Size: 1305 111 BstXI CCAn_nnnn'nTGG Cuts at: 0 501 534 1416 Size: 501 33 882 Fragments arranged by size: 882 501 33 BstYI r'GATC_y Cuts at: 0 463 1337 1396 1416 Size: 463 874 59 20 Fragments arranged by size: 874 463 59 20 Bsu36I CC'TnA_GG Cuts at: 0 418 1416 Size: 418 998 BtgI C'CryG_G Cuts at: 0 914 1416 Size: 914 502 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 183 578 1416 Size: 183 395 838 Fragments arranged by size: 838 395 183 BtsI GCAGTG_nn' Cuts at: 0 27 730 1416 Size: 27 703 686 Fragments arranged by size: 703 686 27 EaeI y'GGCC_r Cuts at: 0 917 1106 1416 Size: 917 189 310 Fragments arranged by size: 917 310 189 EarI CTCTTCn'nnn_ Cuts at: 0 483 689 704 1177 1223 1416 Size: 483 206 15 473 46 193 Fragments arranged by size: 483 473 206 193 46 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 832 1416 Size: 832 584 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 1416 Size: 757 659 EcoO109I rG'GnC_Cy Cuts at: 0 9 1416 Size: 9 1407 EcoRI G'AATT_C Cuts at: 0 124 787 1416 Size: 124 663 629 Fragments arranged by size: 663 629 124 HaeII r_GCGC'y Cuts at: 0 405 1416 Size: 405 1011 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 139 171 667 699 749 781 1334 1366 Size: 139 32 496 32 50 32 553 32 Cuts at: 1366 1416 Size: 50 Fragments arranged by size: 553 496 139 50 50 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 410 600 1416 Size: 410 190 816 Fragments arranged by size: 816 410 190 MspA1I CmG'CkG Cuts at: 0 501 1116 1416 Size: 501 615 300 Fragments arranged by size: 615 501 300 NruI TCG'CGA Cuts at: 0 672 1416 Size: 672 744 PasI CC'CwG_GG Cuts at: 0 264 1354 1416 Size: 264 1090 62 Fragments arranged by size: 1090 264 62 PflMI CCAn_nnn'nTGG Cuts at: 0 1296 1416 Size: 1296 120 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 76 108 1416 Size: 76 32 1308 Fragments arranged by size: 1308 76 32 PpuMI rG'GwC_Cy Cuts at: 0 9 1416 Size: 9 1407 PspOMI G'GGCC_C Cuts at: 0 688 1416 Size: 688 728 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 551 583 1416 Size: 551 32 833 Fragments arranged by size: 833 551 32 PstI C_TGCA'G Cuts at: 0 986 1380 1416 Size: 986 394 36 Fragments arranged by size: 986 394 36 PvuI CG_AT'CG Cuts at: 0 670 1416 Size: 670 746 PvuII CAG'CTG Cuts at: 0 1116 1416 Size: 1116 300 SapI GCTCTTCn'nnn_ Cuts at: 0 689 1416 Size: 689 727 SfcI C'TryA_G Cuts at: 0 47 982 1376 1416 Size: 47 935 394 40 Fragments arranged by size: 935 394 47 40 SmaI CCC'GGG Cuts at: 0 121 178 454 1416 Size: 121 57 276 962 Fragments arranged by size: 962 276 121 57 SmlI C'TyrA_G Cuts at: 0 1156 1416 Size: 1156 260 StuI AGG'CCT Cuts at: 0 869 1024 1416 Size: 869 155 392 Fragments arranged by size: 869 392 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 435 1183 1416 Size: 435 748 233 Fragments arranged by size: 748 435 233 TatI w'GTAC_w Cuts at: 0 827 1416 Size: 827 589 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 375 407 1416 Size: 375 32 1009 Fragments arranged by size: 1009 375 32 Tth111I GACn'n_nGTC Cuts at: 0 153 1416 Size: 153 1263 XmaI C'CCGG_G Cuts at: 0 119 176 452 1416 Size: 119 57 276 964 Fragments arranged by size: 964 276 119 57 ZraI GAC'GTC Cuts at: 0 848 1416 Size: 848 568 Enzymes that do cut: AatII AccI AclI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI MspA1I NruI PasI PflMI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII SapI SfcI SmaI SmlI StuI TaqII TatI TstI Tth111I XmaI ZraI Enzymes that do not cut: AarI Acc65I AcuI AfeI AflII AflIII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII BmtI BplI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrGI BssHII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI XbaI XcmI XhoI XmnI