Restriction map for
pFN21AE2481
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AflII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NsiI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tru9I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 846 GACGTC GACGT^C
AccI 45 GTCTAC GT^[AC][GT]AC
AccIII 1393 TCCGGA T^CCGGA
AclI 1067 AACGTT AA^CGTT
AcyI 846 GACGTC G[GA]^CG[CT]C
Alw44I 991 GTGCAC G^TGCAC
AlwNI 7 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 417 CCTGAGG CC^T[ACGT]AGG
ApaI 688 GGGCCC GGGCC^C
ApaLI 991 GTGCAC G^TGCAC
AspHI 991 GTGCAC G[AT]GC[AT]^C
AspI 150 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 301 CCTCAGC CCTCAGC(-5/-2)
BfaI 811 CTAG C^TAG
BglI 260 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 583 GCTCAGC GC^T[ACGT]AGC
BpmI 736 CTGGAG CTGGAG(16/14)
Bpu1102I 583 GCTCAGC GC^T[ACGT]AGC
BsaHI 846 GACGTC G[GA]^CG[CT]C
BseAI 1393 TCCGGA T^CCGGA
BsiEI 667 CGATCG CG[GA][CT]^CG
BsiHKAI 991 GTGCAC G[AT]GC[AT]^C
BspEI 1393 TCCGGA T^CCGGA
BsrBI 270 CCGCTC CCGCTC(-3/-3)
BsrDI 701 CATTGC GCAATG(2/0)
BstBI 678 TTCGAA TT^CGAA
BstEII 1305 GGTGACC G^GT[ACGT]ACC
BstPI 1305 GGTGACC G^GT[ACGT]ACC
Bsu36I 417 CCTGAGG CC^T[ACGT]AGG
CelII 583 GCTCAGC GC^T[ACGT]AGC
DraII 8 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 914 CCGTGG C^C[GA][CT]GG
Eco81I 417 CCTGAGG CC^T[ACGT]AGG
EcoO109I 8 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1305 GGTGACC G^GT[ACGT]ACC
HaeII 401 AGCGCC [GA]GCGC^[CT]
Hin1I 846 GACGTC G[GA]^CG[CT]C
MaeI 811 CTAG C^TAG
MaeIII 1306 GTGAC ^GT[ACGT]AC
MroI 1393 TCCGGA T^CCGGA
NruI 670 TCGCGA TCG^CGA
NspV 678 TTCGAA TT^CGAA
PflFI 150 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1290 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 8 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1067 AACGTT AA^CGTT
PvuI 667 CGATCG CGAT^CG
PvuII 1114 CAGCTG CAG^CTG
SapI 694 GAAGAGC GCTCTTC(1/4)
SfuI 678 TTCGAA TT^CGAA
SmlI 1156 CTCAAG C^T[CT][GA]AG
Tsp45I 1306 GTGAC ^GT[GC]AC
Tth111I 150 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1290 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 124 GAATTC [GA]^AATT[CT]
787 GAATTC
ApoI 124 GAATTC [GA]^AATT[CT]
787 GAATTC
BamHI 463 GGATCC G^GATCC
1396 GGATCC
BsaWI 1360 ACCGGA [AT]^CCGG[AT]
1393 TCCGGA
BsgI 109 CTGCAC GTGCAG(16/14)
895 CTGCAC
BspMI 821 ACCTGC ACCTGC(4/8)
6 GCAGGT
BstAPI 6 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
984 GCAGGCCGTGC
BstXI 494 CCACCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
527 CCAACAGATTGG
EaeI 917 TGGCCG [CT]^GGCC[GA]
1106 CGGCCA
EcoRI 124 GAATTC G^AATTC
787 GAATTC
MspA1I 499 CCGCTG C[AC]G^C[GT]G
1114 CAGCTG
PstI 982 CTGCAG CTGCA^G
1376 CTGCAG
SfaNI 332 GCATC GCATC(5/9)
905 GCATC
StuI 867 AGGCCT AGG^CCT
1022 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI SmaI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI