(Circular) (Six-base) MAPSORT of: pFN21AE2481.seq Check: 7816 from: 1 to: 6121 pFN21AE2481 6121 bp With 182 enzymes: SgfI * August 14, 2013 14:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2855 3931 278 Size: 53 83 186 936 1319 1076 2468 Fragments arranged by size: 2468 1319 1076 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2051 3464 1937 Size: 114 1413 4594 Fragments arranged by size: 4594 1413 114 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 3073 4248 4621 1563 Size: 1510 1175 373 3063 Fragments arranged by size: 3063 1510 1175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4184 5568 5836 1632 Size: 2552 1384 268 1917 Fragments arranged by size: 2552 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3002 1184 Size: 1818 4303 AlwNI CAG_nnn'CTG Cuts at: 2017 3864 5436 2017 Size: 1847 1572 2702 Fragments arranged by size: 2702 1847 1572 ApaI G_GGCC'C Cuts at: 2697 2697 Size: 6121 ApaLI G'TGCA_C Cuts at: 2996 4178 5334 2996 Size: 1182 1156 3783 Fragments arranged by size: 3783 1182 1156 ApoI r'AATT_y Cuts at: 1493 2129 2792 3430 3656 3808 5917 1493 Size: 636 663 638 226 152 2109 1697 Fragments arranged by size: 2109 1697 663 638 636 226 152 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 2124 2181 2457 2603 3057 3446 1870 Size: 73 181 57 276 146 454 389 4545 Fragments arranged by size: 4545 454 389 276 181 146 73 57 BamHI G'GATC_C Cuts at: 2468 3401 3451 3818 2468 Size: 933 50 367 4771 Fragments arranged by size: 4771 933 367 50 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2954 3200 3442 4897 Size: 325 217 627 387 780 246 242 1455 Cuts at: 4897 618 Size: 1842 Fragments arranged by size: 1842 1455 780 627 387 325 246 242 217 BanII G_rGCy'C Cuts at: 1391 2228 2636 2697 3373 3440 1391 Size: 837 408 61 676 67 4072 Fragments arranged by size: 4072 837 676 408 67 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2017 2424 2517 2857 3150 928 Size: 971 118 407 93 340 293 3899 Fragments arranged by size: 3899 971 407 340 293 118 93 BbvCI CC'TCA_GC Cuts at: 2307 2307 Size: 6121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 4732 136 Size: 107 122 71 631 605 599 2461 1525 Fragments arranged by size: 2461 1525 631 605 599 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 2589 3538 2589 Size: 949 5172 Bme1580I G_kGCm'C Cuts at: 1790 2177 2697 3000 4182 5338 1790 Size: 387 520 303 1182 1156 2573 Fragments arranged by size: 2573 1182 1156 520 387 303 BmgBI CAC'GTC Cuts at: 1318 2160 1318 Size: 842 5279 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 3852 4810 Size: 416 53 83 399 24 762 1673 958 Cuts at: 4810 442 Size: 1753 Fragments arranged by size: 1753 1673 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2762 4781 1239 Size: 1523 2019 2579 Fragments arranged by size: 2579 2019 1523 Bpu10I CC'TnA_GC Cuts at: 2307 2348 2307 Size: 41 6080 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3146 4246 5111 5409 3146 Size: 1100 865 298 3858 Fragments arranged by size: 3858 1100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2147 2289 2887 3109 4784 882 Size: 617 648 142 598 222 1675 2219 Fragments arranged by size: 2219 1675 648 617 598 222 142 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2852 3928 4310 4945 Size: 53 83 186 936 1319 1076 382 635 Cuts at: 4945 275 Size: 1451 Fragments arranged by size: 1451 1319 1076 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3365 3398 4554 4952 5226 5373 5784 3365 Size: 33 1156 398 274 147 411 3702 Fragments arranged by size: 3702 1156 411 398 274 147 33 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2380 2410 1178 Size: 30 165 30 741 30 206 30 4889 Fragments arranged by size: 4889 741 206 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2050 2153 2162 2165 2879 3164 1186 Size: 864 103 9 3 714 285 4143 Fragments arranged by size: 4143 864 714 285 103 9 3 BseYI C'CCAG_C Cuts at: 2312 2342 2448 2555 2619 5324 5820 2312 Size: 30 106 107 64 2705 496 2613 Fragments arranged by size: 2705 2613 496 107 106 64 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2885 1163 Size: 573 363 786 4399 Fragments arranged by size: 4399 786 573 363 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2675 3571 4332 5360 1540 Size: 275 186 674 896 761 1028 2301 Fragments arranged by size: 2301 1028 896 761 674 275 186 BsiHKAI G_wGCw'C Cuts at: 3000 4182 4267 5338 3000 Size: 1182 85 1071 3783 Fragments arranged by size: 3783 1182 1071 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2228 2636 2697 3000 3373 3440 Size: 399 387 51 408 61 303 373 67 Cuts at: 3440 4182 4267 5338 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 387 373 303 85 67 61 51 BspEI T'CCGG_A Cuts at: 3398 5784 3398 Size: 2386 3735 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2002 2835 3476 844 Size: 1158 833 641 3489 Fragments arranged by size: 3489 1158 833 641 BsrBI CCG'CTC Cuts at: 1394 1823 2277 4008 1394 Size: 429 454 1731 3507 Fragments arranged by size: 3507 1731 454 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2705 4616 4790 65 Size: 1183 1457 1911 174 1396 Fragments arranged by size: 1911 1457 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2651 3270 4765 4952 1700 Size: 110 210 631 619 1495 187 2869 Fragments arranged by size: 2869 1495 631 619 210 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 2292 2845 3149 4181 5193 2292 Size: 553 304 1032 1012 3220 Fragments arranged by size: 3220 1032 1012 553 304 BstAPI GCAn_nnn'nTGC Cuts at: 794 2017 2995 794 Size: 1223 978 3920 Fragments arranged by size: 3920 1223 978 BstBI TT'CG_AA Cuts at: 2684 4925 2684 Size: 2241 3880 BstEII G'GTnAC_C Cuts at: 1184 3310 1184 Size: 2126 3995 BstXI CCAn_nnnn'nTGG Cuts at: 2506 2539 2506 Size: 33 6088 BstYI r'GATC_y Cuts at: 1972 2468 3342 3401 3451 3818 4203 4220 5672 Size: 496 874 59 50 367 385 17 1452 Cuts at: 5672 5758 5770 6116 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 874 496 385 367 346 86 59 50 17 12 Bsu36I CC'TnA_GG Cuts at: 2423 2423 Size: 6121 BtgI C'CryG_G Cuts at: 513 1065 2004 2919 513 Size: 552 939 915 3715 Fragments arranged by size: 3715 939 915 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2188 2583 487 Size: 683 56 962 395 4025 Fragments arranged by size: 4025 962 683 395 56 BtsI GCAGTG_nn' Cuts at: 2032 2735 3637 4431 4451 2032 Size: 703 902 794 20 3702 Fragments arranged by size: 3702 902 794 703 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2922 3111 3568 Size: 54 1425 50 275 18 1092 189 457 Cuts at: 3568 4457 6086 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1092 889 457 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2488 2694 2709 3182 3228 4051 1353 Size: 1135 206 15 473 46 823 3423 Fragments arranged by size: 3423 1135 823 473 206 46 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2837 4708 5082 5228 387 Size: 1064 138 1248 1871 374 146 1280 Fragments arranged by size: 1871 1280 1248 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2762 4184 4781 5568 5836 1239 Size: 393 1130 1422 597 787 268 1524 Fragments arranged by size: 1524 1422 1130 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2014 2014 Size: 6121 EcoRI G'AATT_C Cuts at: 2129 2792 3430 3808 2129 Size: 663 638 378 4442 Fragments arranged by size: 4442 663 638 378 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 2410 5268 1678 Size: 732 2858 2531 Fragments arranged by size: 2858 2531 732 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2176 2672 2704 2754 Size: 32 135 32 572 32 496 32 50 Cuts at: 2754 2786 3339 3371 4762 4794 4836 4868 1373 Size: 32 553 32 1391 32 42 32 2626 Fragments arranged by size: 2626 1391 572 553 496 135 50 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2415 2605 5235 5419 1305 Size: 1110 190 2630 184 2007 Fragments arranged by size: 2630 2007 1110 190 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4299 Fragments arranged by size: 4299 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4079 4438 4597 518 Size: 648 2913 359 159 2042 Fragments arranged by size: 2913 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2506 3121 3537 4214 5362 5607 1606 Size: 900 615 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 900 677 615 416 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4630 Fragments arranged by size: 4630 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NruI TCG'CGA Cuts at: 2677 2677 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 3479 5024 1132 Size: 97 2250 1545 2229 Fragments arranged by size: 2250 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2269 3359 1348 Size: 921 1090 4110 Fragments arranged by size: 4110 1090 921 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 3301 1108 Size: 2193 3928 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2081 2113 4190 4222 5703 5735 2081 Size: 32 2077 32 1481 32 2467 Fragments arranged by size: 2467 2077 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2014 2014 Size: 6121 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PspOMI G'GGCC_C Cuts at: 2693 2693 Size: 6121 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2556 2588 2556 Size: 32 6089 PstI C_TGCA'G Cuts at: 830 2991 3385 3473 830 Size: 2161 394 88 3478 Fragments arranged by size: 3478 2161 394 88 PvuI CG_AT'CG Cuts at: 2001 2675 2001 Size: 674 5447 PvuII CAG'CTG Cuts at: 3121 3121 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SapI GCTCTTCn'nnn_ Cuts at: 2694 2694 Size: 6121 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2052 2987 3381 3469 4604 5285 5476 Size: 219 1007 935 394 88 1135 681 191 Cuts at: 5476 826 Size: 1471 Fragments arranged by size: 1471 1135 1007 935 681 394 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 2126 2183 2459 3448 1872 Size: 254 57 276 989 4545 Fragments arranged by size: 4545 989 276 254 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 3161 4225 5126 5388 820 Size: 197 926 1218 1064 901 262 1553 Fragments arranged by size: 1553 1218 1064 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 2874 3029 1517 Size: 1357 155 4609 Fragments arranged by size: 4609 1357 155 StyI C'CwwG_G Cuts at: 513 1065 2004 3560 513 Size: 552 939 1556 3074 Fragments arranged by size: 3074 1556 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2440 3188 4148 4165 4318 4503 4948 Size: 1255 860 748 960 17 153 185 445 Cuts at: 4948 325 Size: 1498 Fragments arranged by size: 1498 1255 960 860 748 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4367 6079 Size: 274 80 33 51 1443 855 1535 1712 Cuts at: 6079 96 Size: 138 Fragments arranged by size: 1712 1535 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2380 2412 6081 6113 2380 Size: 32 3669 32 2388 Fragments arranged by size: 3669 2388 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2158 1316 Size: 842 5279 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 2124 2181 2457 3446 1870 Size: 254 57 276 989 4545 Fragments arranged by size: 4545 989 276 254 57 XmnI GAAnn'nnTTC Cuts at: 4250 4250 Size: 6121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2853 3929 276 Size: 53 83 186 936 1319 1076 2468 Fragments arranged by size: 2468 1319 1076 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI