TRMT12 ORF clone pFN21AE2265

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2265

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Clone info.

Species

human

Clone Name

pFN21AE2265 Sequence (with vector) Restriction Map

Product ID

FHC12544

ORF Sequence

DNA sequence (1344 bp)
Protein sequence (448 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2265

Symbol

TRMT12

Alias : TRM12, TYW2

Description

tRNA methyltransferase 12 homolog (S. cerevisiae)

Original Clone ID

cs03365

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1344 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 448 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6349.1 1e-192 99.8 TRMT12 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003402 183 316 PF02475 Protein of unknown function Met10
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060426 448 611244 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2265 Download

>KIEE2265  1344 bp
ATGAGAGAGAATGTGGTTGTTAGCAACATGGAGAGAGAAAGTGGGAAGCCCGTGGCTGTT
GTCGCAGTTGTGACTGAGCCTCGGTTTACCCAGCGATACAGAGAATATCTCCAGAGGCAG
AAACTCTTTGATACACAGCACCGTGTGGAAAAGATGCCGGATGGCTCGGTGGCGCTACCG
GTGCTGGGAGAGACGCTTCCAGAGCAGCACCTGCAGGAGCTGAGGAATCGTGTTGCCCCA
GGCAGTCCCTGTATGCTCACGCAGCTCCCGGATCCTGTTCCTTCGAAGAGGGCCCAGGGT
TGTTCACCTGCCCAAAAATTGTGTCTTGAGGTGAGTCGCTGGGTGGAGGGTCGGGGAGTC
AAGTGGTCAGCCGAGTTGGAGGCTGATTTGCCCCGATCATGGCAACGGCATGGTAATCTC
TTGTTGCTGAGTGAAGACTGTTTCCAAGCCAAGCAGTGGAAAAATCTGGGACCGGAACTC
TGGGAGACCGTTGCCTTGGCACTTGGCGTCCAGCGTTTGGCAAAACGAGGGCGGGTATCA
CCGGATGGTACTCGAACTCCAGCAGTGACACTGCTGCTGGGTGACCATGGCTGGGTAGAG
CATGTGGATAATGGTATCCGTTATAAGTTTGACGTGACCCAGTGTATGTTCTCCTTTGGA
AACATCACTGAGAAGCTTCGAGTGGCATCGTTGTCCTGTGCTGGAGAAGTGCTGGTGGAT
CTCTATGCAGGGATTGGTTATTTTACATTGCCTTTCCTAGTTCATGCTGGTGCTGCCTTC
GTCCATGCTTGTGAGTGGAATCCCCATGCTGTAGTTGCTCTGAGAAATAACCTTGAGATC
AATGGAGTAGCAGATCGGTGCCAAATACACTTTGGAGATAACAGAAAACTGAAGCTCTCA
AATATTGCAGATAGGGTGATCCTGGGGCTGATTCCCAGCTCTGAAGAAGGCTGGCCCATT
GCCTGCCAAGTGTTAAGGCAGGATGCTGGAGGCATTTTGCATATCCACCAAAATGTGGAA
TCTTTCCCAGGGAAGAATCTTCAGGCTCTTGGAGTCAGCAAAGTAGAGAAAGAGCATTGG
CTGTATCCTCAGCAAATTACCACCAACCAATGGAAAAATGGAGCTACCAGGGATTCTAGG
GGAAAAATGCTGTCACCAGCCACCAAGCCAGAGTGGCAAAGGTGGGCAGAATCTGCAGAA
ACTCGAATCGCCACTCTTCTTCAGCAGGTGCATGGGAAACCATGGAAGACACAAATTCTG
CACATCCAACCAGTGAAATCCTATGCTCCCCATGTGGATCACATAGTCCTGGATCTGGAA
TGCTGCCCCTGTCCTTCAGTTGGC

ORF Protein

Cloned ORF protein sequence for pFN21AE2265 Download

>KIEE2265  448 aa
MRENVVVSNMERESGKPVAVVAVVTEPRFTQRYREYLQRQKLFDTQHRVEKMPDGSVALP
VLGETLPEQHLQELRNRVAPGSPCMLTQLPDPVPSKRAQGCSPAQKLCLEVSRWVEGRGV
KWSAELEADLPRSWQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVS
PDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVD
LYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLS
NIADRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHW
LYPQQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQIL
HIQPVKSYAPHVDHIVLDLECCPCPSVG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2265 Download

>pFN21AE2265  6049 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGAGAGAATGTGGTTGTTAGCAACATGGAGAG
AGAAAGTGGGAAGCCCGTGGCTGTTGTCGCAGTTGTGACTGAGCCTCGGTTTACCCAGCG
ATACAGAGAATATCTCCAGAGGCAGAAACTCTTTGATACACAGCACCGTGTGGAAAAGAT
GCCGGATGGCTCGGTGGCGCTACCGGTGCTGGGAGAGACGCTTCCAGAGCAGCACCTGCA
GGAGCTGAGGAATCGTGTTGCCCCAGGCAGTCCCTGTATGCTCACGCAGCTCCCGGATCC
TGTTCCTTCGAAGAGGGCCCAGGGTTGTTCACCTGCCCAAAAATTGTGTCTTGAGGTGAG
TCGCTGGGTGGAGGGTCGGGGAGTCAAGTGGTCAGCCGAGTTGGAGGCTGATTTGCCCCG
ATCATGGCAACGGCATGGTAATCTCTTGTTGCTGAGTGAAGACTGTTTCCAAGCCAAGCA
GTGGAAAAATCTGGGACCGGAACTCTGGGAGACCGTTGCCTTGGCACTTGGCGTCCAGCG
TTTGGCAAAACGAGGGCGGGTATCACCGGATGGTACTCGAACTCCAGCAGTGACACTGCT
GCTGGGTGACCATGGCTGGGTAGAGCATGTGGATAATGGTATCCGTTATAAGTTTGACGT
GACCCAGTGTATGTTCTCCTTTGGAAACATCACTGAGAAGCTTCGAGTGGCATCGTTGTC
CTGTGCTGGAGAAGTGCTGGTGGATCTCTATGCAGGGATTGGTTATTTTACATTGCCTTT
CCTAGTTCATGCTGGTGCTGCCTTCGTCCATGCTTGTGAGTGGAATCCCCATGCTGTAGT
TGCTCTGAGAAATAACCTTGAGATCAATGGAGTAGCAGATCGGTGCCAAATACACTTTGG
AGATAACAGAAAACTGAAGCTCTCAAATATTGCAGATAGGGTGATCCTGGGGCTGATTCC
CAGCTCTGAAGAAGGCTGGCCCATTGCCTGCCAAGTGTTAAGGCAGGATGCTGGAGGCAT
TTTGCATATCCACCAAAATGTGGAATCTTTCCCAGGGAAGAATCTTCAGGCTCTTGGAGT
CAGCAAAGTAGAGAAAGAGCATTGGCTGTATCCTCAGCAAATTACCACCAACCAATGGAA
AAATGGAGCTACCAGGGATTCTAGGGGAAAAATGCTGTCACCAGCCACCAAGCCAGAGTG
GCAAAGGTGGGCAGAATCTGCAGAAACTCGAATCGCCACTCTTCTTCAGCAGGTGCATGG
GAAACCATGGAAGACACAAATTCTGCACATCCAACCAGTGAAATCCTATGCTCCCCATGT
GGATCACATAGTCCTGGATCTGGAATGCTGCCCCTGTCCTTCAGTTGGCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT