Restriction map for
pFN21AE2265
(Linear) (Six-base) MAPSORT of: KIEE2265.seq Check: 4392 from: 1 to: 1344
KIEE2265.orf
With 182 enzymes: SgfI *
July 3, 2012 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 218 315 1215 1344
Size: 218 97 900 129
Fragments arranged by size:
900 218 129 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 962 1024 1204 1319 1344
Size: 910 52 62 180 115 25
Fragments arranged by size:
910 180 115 62 52 25
AgeI A'CCGG_T
Cuts at: 0 177 1344
Size: 177 1167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 873 1344
Size: 873 471
AlwNI CAG_nnn'CTG
Cuts at: 0 210 219 248 1192 1344
Size: 210 9 29 944 152
Fragments arranged by size:
944 210 152 29 9
ApaI G_GGCC'C
Cuts at: 0 294 1344
Size: 294 1050
ApoI r'AATT_y
Cuts at: 0 1253 1344
Size: 1253 91
BamHI G'GATC_C
Cuts at: 0 270 1344
Size: 270 1074
BanI G'GyrC_C
Cuts at: 0 857 1344
Size: 857 487
BanII G_rGCy'C
Cuts at: 0 294 1344
Size: 294 1050
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 1252 1344
Size: 440 812 92
Fragments arranged by size:
812 440 92
BbvCI CC'TCA_GC
Cuts at: 0 220 1088 1344
Size: 220 868 256
Fragments arranged by size:
868 256 220
BciVI GTATCCnnnnn_n'
Cuts at: 0 625 1094 1344
Size: 625 469 250
Fragments arranged by size:
625 469 250
BglI GCCn_nnn'nGGC
Cuts at: 0 1173 1344
Size: 1173 171
Bme1580I G_kGCm'C
Cuts at: 0 294 1344
Size: 294 1050
BmgBI CAC'GTC
Cuts at: 0 633 1344
Size: 633 711
BmrI ACTGGGnnnn_n'
Cuts at: 0 633 1344
Size: 633 711
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 124 1344
Size: 124 1220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 542 722 1007 1344
Size: 94 448 180 285 337
Fragments arranged by size:
448 337 285 180 94
Bpu10I CC'TnA_GC
Cuts at: 0 220 1088 1344
Size: 220 868 256
Fragments arranged by size:
868 256 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 853 1344
Size: 346 507 491
Fragments arranged by size:
507 491 346
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 1344
Size: 478 866
BsaHI Gr'CG_yC
Cuts at: 0 506 1344
Size: 506 838
BsaWI w'CCGG_w
Cuts at: 0 177 471 540 1344
Size: 177 294 69 804
Fragments arranged by size:
804 294 177 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 540 570 1344
Size: 540 30 774
Fragments arranged by size:
774 540 30
BseYI C'CCAG_C
Cuts at: 0 89 183 338 576 590 934 1344
Size: 89 94 155 238 14 344 410
Fragments arranged by size:
410 344 238 155 94 89 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1344
Size: 1243 101
BsmI GAATG_Cn'
Cuts at: 0 1324 1344
Size: 1324 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 184 1344
Size: 184 1160
Bsp1286I G_dGCh'C
Cuts at: 0 294 1344
Size: 294 1050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 315 1215 1344
Size: 218 97 900 129
Fragments arranged by size:
900 218 129 97
BsrDI GCAATG_nn'
Cuts at: 0 745 956 1344
Size: 745 211 388
Fragments arranged by size:
745 388 211
BsrFI r'CCGG_y
Cuts at: 0 177 1344
Size: 177 1167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 210 1192 1344
Size: 210 982 152
Fragments arranged by size:
982 210 152
BstBI TT'CG_AA
Cuts at: 0 283 1344
Size: 283 1061
BstEII G'GTnAC_C
Cuts at: 0 580 1344
Size: 580 764
BstYI r'GATC_y
Cuts at: 0 270 717 1311 1344
Size: 270 447 594 33
Fragments arranged by size:
594 447 270 33
BtgI C'CryG_G
Cuts at: 0 50 585 1240 1344
Size: 50 535 655 104
Fragments arranged by size:
655 535 104 50
BtsI GCAGTG_nn'
Cuts at: 0 460 568 569 1344
Size: 460 108 1 775
Fragments arranged by size:
775 460 108 1
DraIII CAC_nnn'GTG
Cuts at: 0 144 1344
Size: 144 1200
EarI CTCTTCn'nnn_
Cuts at: 0 280 1220 1344
Size: 280 940 124
Fragments arranged by size:
940 280 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 542 722 910 962 1007 1024 1204
Size: 94 448 180 188 52 45 17 180
Cuts at: 1204 1319 1344
Size: 115 25
Fragments arranged by size:
448 188 180 180 115 94 52 45
25 17
EcoO109I rG'GnC_Cy
Cuts at: 0 290 1344
Size: 290 1054
HaeII r_GCGC'y
Cuts at: 0 175 1344
Size: 175 1169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 461 493 1344
Size: 63 32 366 32 851
Fragments arranged by size:
851 366 63 32 32
HindIII A'AGCT_T
Cuts at: 0 673 1344
Size: 673 671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 1290 1344
Size: 356 934 54
Fragments arranged by size:
934 356 54
MslI CAynn'nnrTG
Cuts at: 0 767 788 1010 1344
Size: 767 21 222 334
Fragments arranged by size:
767 334 222 21
NcoI C'CATG_G
Cuts at: 0 585 1240 1344
Size: 585 655 104
Fragments arranged by size:
655 585 104
NspI r_CATG'y
Cuts at: 0 604 1344
Size: 604 740
PasI CC'CwG_GG
Cuts at: 0 294 1027 1344
Size: 294 733 317
Fragments arranged by size:
733 317 294
PflMI CCAn_nnn'nTGG
Cuts at: 0 455 516 1014 1109 1344
Size: 455 61 498 95 235
Fragments arranged by size:
498 455 235 95 61
PfoI T'CCnGG_A
Cuts at: 0 266 1307 1344
Size: 266 1041 37
Fragments arranged by size:
1041 266 37
PsiI TTA'TAA
Cuts at: 0 623 1344
Size: 623 721
PspOMI G'GGCC_C
Cuts at: 0 290 1344
Size: 290 1054
PstI C_TGCA'G
Cuts at: 0 215 1197 1344
Size: 215 982 147
Fragments arranged by size:
982 215 147
SbfI CC_TGCA'GG
Cuts at: 0 215 1344
Size: 215 1129
SfcI C'TryA_G
Cuts at: 0 211 809 1193 1344
Size: 211 598 384 151
Fragments arranged by size:
598 384 211 151
SmlI C'TyrA_G
Cuts at: 0 325 832 1344
Size: 325 507 512
Fragments arranged by size:
512 507 325
SspI AAT'ATT
Cuts at: 0 903 1344
Size: 903 441
StyI C'CwwG_G
Cuts at: 0 494 585 1240 1344
Size: 494 91 655 104
Fragments arranged by size:
655 494 104 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 330 568 1344
Size: 330 238 776
Fragments arranged by size:
776 330 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 1344
Size: 491 32 821
Fragments arranged by size:
821 491 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 868 1114 1344
Size: 868 246 230
Fragments arranged by size:
868 246 230
Enzymes that do cut:
AarI AcuI AgeI AlfI AlwNI ApaI ApoI BamHI
BanI BanII BbsI BbvCI BciVI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseYI BsgI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstBI BstEII BstYI BtgI BtsI DraIII
EarI Eco57MI EcoO109I HaeII Hin4I HindIII MmeI MslI
NcoI NspI PasI PflMI PfoI PsiI PspOMI PstI
SbfI SfcI SmlI SspI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BclI BfrBI BglII BlpI BmtI
BsaAI BsaBI BseRI BsiEI BsiHKAI BsiWI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI