(Circular) (Six-base) MAPSORT of: pFN21AE2265.seq Check: 7641 from: 1 to: 6049 pFN21AE2265 6049 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2223 2320 3220 2223 Size: 97 900 5052 Fragments arranged by size: 5052 900 97 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3392 1937 Size: 1455 4594 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2915 2967 3029 3209 3324 4112 5496 5764 Size: 1283 52 62 180 115 788 1384 268 Cuts at: 5764 1632 Size: 1917 Fragments arranged by size: 1917 1384 1283 788 268 180 115 62 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 4948 5925 1203 Size: 3745 977 1327 Fragments arranged by size: 3745 1327 977 AgeI A'CCGG_T Cuts at: 2182 4880 2182 Size: 2698 3351 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2878 1184 Size: 1694 4355 AlwNI CAG_nnn'CTG Cuts at: 2215 2224 2253 3197 3792 5364 2215 Size: 9 29 944 595 1572 2900 Fragments arranged by size: 2900 1572 944 595 29 9 ApaI G_GGCC'C Cuts at: 2299 2299 Size: 6049 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 3258 3358 3584 3736 5845 1493 Size: 1765 100 226 152 2109 1697 Fragments arranged by size: 2109 1765 1697 226 152 100 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 3374 1870 Size: 73 1431 4545 Fragments arranged by size: 4545 1431 73 BamHI G'GATC_C Cuts at: 2275 3379 3746 2275 Size: 1104 367 4578 Fragments arranged by size: 4578 1104 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2862 3370 4825 618 Size: 325 217 627 1075 508 1455 1842 Fragments arranged by size: 1842 1455 1075 627 508 325 217 BanII G_rGCy'C Cuts at: 1391 2299 3368 1391 Size: 908 1069 4072 Fragments arranged by size: 4072 1069 908 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2445 3257 928 Size: 971 546 812 3720 Fragments arranged by size: 3720 971 812 546 BbvCI CC'TCA_GC Cuts at: 2225 3093 2225 Size: 868 5181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4240 4274 1903 Size: 34 2303 34 3678 Fragments arranged by size: 3678 2303 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2630 3099 3941 5151 2630 Size: 469 842 1210 3528 Fragments arranged by size: 3528 1210 842 469 BclI T'GATC_A Cuts at: 1548 1548 Size: 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3178 4660 136 Size: 107 122 71 631 605 1506 1482 1525 Fragments arranged by size: 1525 1506 1482 631 605 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 3466 3466 Size: 6049 Bme1580I G_kGCm'C Cuts at: 1790 2299 4110 5266 1790 Size: 509 1811 1156 2573 Fragments arranged by size: 2573 1811 1156 509 BmgBI CAC'GTC Cuts at: 1318 2638 1318 Size: 1320 4729 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2638 3780 4738 Size: 416 53 83 399 24 1221 1142 958 Cuts at: 4738 442 Size: 1753 Fragments arranged by size: 1753 1221 1142 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2129 2129 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2547 2727 3012 4709 1239 Size: 860 448 180 285 1697 2579 Fragments arranged by size: 2579 1697 860 448 285 180 Bpu10I CC'TnA_GC Cuts at: 2225 3093 2225 Size: 868 5181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2351 2858 4174 5039 5337 2351 Size: 507 1316 865 298 3063 Fragments arranged by size: 3063 1316 865 507 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2483 4712 882 Size: 617 984 2229 2219 Fragments arranged by size: 2229 2219 984 617 BsaAI yAC'GTr Cuts at: 493 1204 3840 493 Size: 711 2636 2702 Fragments arranged by size: 2702 2636 711 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2511 3856 4238 4873 Size: 53 83 186 936 978 1345 382 635 Cuts at: 4873 275 Size: 1451 Fragments arranged by size: 1451 1345 978 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2182 2476 2545 4482 4880 5154 5301 5712 2182 Size: 294 69 1937 398 274 147 411 2519 Fragments arranged by size: 2519 1937 411 398 294 274 147 69 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2545 2575 1178 Size: 30 165 30 1142 30 4652 Fragments arranged by size: 4652 1142 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6049 BseYI C'CCAG_C Cuts at: 2094 2188 2343 2581 2595 2939 5252 5748 2094 Size: 94 155 238 14 344 2313 496 2395 Fragments arranged by size: 2395 2313 496 344 238 155 94 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3248 1163 Size: 573 1512 3964 Fragments arranged by size: 3964 1512 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 4260 5288 1540 Size: 275 186 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4110 4195 5266 4110 Size: 85 1071 4893 Fragments arranged by size: 4893 1071 85 BsmI GAATG_Cn' Cuts at: 3329 3560 3653 3329 Size: 231 93 5725 Fragments arranged by size: 5725 231 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2189 2189 Size: 6049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2299 3368 4110 4195 5266 1391 Size: 399 509 1069 742 85 1071 2174 Fragments arranged by size: 2174 1071 1069 742 509 399 85 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 2320 3220 3404 844 Size: 1379 97 900 184 3489 Fragments arranged by size: 3489 1379 900 184 97 BsrBI CCG'CTC Cuts at: 1394 1823 3936 1394 Size: 429 2113 3507 Fragments arranged by size: 3507 2113 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2750 2961 4544 4718 65 Size: 1183 1502 211 1583 174 1396 Fragments arranged by size: 1583 1502 1396 1183 211 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2182 4693 4880 1700 Size: 110 372 2511 187 2869 Fragments arranged by size: 2869 2511 372 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 4109 5121 4109 Size: 1012 5037 BstAPI GCAn_nnn'nTGC Cuts at: 794 2215 3197 794 Size: 1421 982 3646 Fragments arranged by size: 3646 1421 982 BstBI TT'CG_AA Cuts at: 2288 4853 2288 Size: 2565 3484 BstEII G'GTnAC_C Cuts at: 1184 2585 1184 Size: 1401 4648 BstYI r'GATC_y Cuts at: 1972 2275 2722 3316 3379 3746 4131 4148 5600 Size: 303 447 594 63 367 385 17 1452 Cuts at: 5600 5686 5698 6044 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 594 447 385 367 346 303 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2055 2590 3245 513 Size: 552 990 535 655 3317 Fragments arranged by size: 3317 990 655 552 535 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 2465 2573 2574 3565 4359 4379 2465 Size: 108 1 991 794 20 4135 Fragments arranged by size: 4135 991 794 108 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2149 2149 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3496 4385 6014 Size: 54 1425 50 275 18 1666 889 1629 Cuts at: 6014 8 Size: 43 Fragments arranged by size: 1666 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 3225 3979 1353 Size: 932 940 754 3423 Fragments arranged by size: 3423 940 932 754 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2547 2727 2915 2967 3012 3029 Size: 393 467 448 180 188 52 45 17 Cuts at: 3029 3209 3324 4112 4709 5496 5764 1239 Size: 180 115 788 597 787 268 1524 Fragments arranged by size: 1524 788 787 597 467 448 393 268 188 180 180 115 52 45 17 EcoO109I rG'GnC_Cy Cuts at: 2295 2295 Size: 6049 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2180 5196 1678 Size: 502 3016 2531 Fragments arranged by size: 3016 2531 502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 2466 2498 4690 Size: 32 135 32 496 32 366 32 2192 Cuts at: 4690 4722 4764 4796 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2192 496 366 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3393 3639 669 Size: 988 281 1455 246 3079 Fragments arranged by size: 3079 1455 988 281 246 HindIII A'AGCT_T Cuts at: 748 2678 748 Size: 1930 4119 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2361 3295 5163 5347 1305 Size: 1056 934 1868 184 2007 Fragments arranged by size: 2007 1868 1056 934 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4227 Fragments arranged by size: 4227 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2772 2793 3015 4007 4366 4525 518 Size: 648 1606 21 222 992 359 159 2042 Fragments arranged by size: 2042 1606 992 648 359 222 159 21 MspA1I CmG'CkG Cuts at: 1606 3465 4142 5290 5535 1606 Size: 1859 677 1148 245 2120 Fragments arranged by size: 2120 1859 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2590 3245 513 Size: 552 1525 655 3317 Fragments arranged by size: 3317 1525 655 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2609 3407 4952 1132 Size: 97 1380 798 1545 2229 Fragments arranged by size: 2229 1545 1380 798 97 PasI CC'CwG_GG Cuts at: 1348 2299 3032 1348 Size: 951 733 4365 Fragments arranged by size: 4365 951 733 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2460 2521 3019 3114 1108 Size: 1352 61 498 95 4043 Fragments arranged by size: 4043 1352 498 95 61 PfoI T'CCnGG_A Cuts at: 2271 3312 2271 Size: 1041 5008 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 2628 3619 2628 Size: 991 5058 PspOMI G'GGCC_C Cuts at: 2295 2295 Size: 6049 PstI C_TGCA'G Cuts at: 830 2220 3202 3401 830 Size: 1390 982 199 3478 Fragments arranged by size: 3478 1390 982 199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SbfI CC_TGCA'GG Cuts at: 2220 3401 2220 Size: 1181 4868 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SfcI C'TryA_G Cuts at: 826 1045 2216 2814 3198 3397 4532 5213 5404 Size: 219 1171 598 384 199 1135 681 191 Cuts at: 5404 826 Size: 1471 Fragments arranged by size: 1471 1171 1135 681 598 384 219 199 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2330 2837 4153 5054 5316 820 Size: 197 926 387 507 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 507 387 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3407 1132 Size: 2275 3774 SspI AAT'ATT Cuts at: 5 52 2908 3973 5 Size: 47 2856 1065 2081 Fragments arranged by size: 2856 2081 1065 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6049 StyI C'CwwG_G Cuts at: 513 1065 2499 2590 3245 3488 513 Size: 552 1434 91 655 243 3074 Fragments arranged by size: 3074 1434 655 552 243 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2335 2573 4076 4093 4246 4431 4876 Size: 1255 755 238 1503 17 153 185 445 Cuts at: 4876 325 Size: 1498 Fragments arranged by size: 1503 1498 1255 755 445 238 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4295 6007 96 Size: 274 80 33 51 1443 2318 1712 138 Fragments arranged by size: 2318 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2496 2528 6009 6041 2496 Size: 32 3481 32 2504 Fragments arranged by size: 3481 2504 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2873 3119 2873 Size: 246 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI