################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BbeI BbrPI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BseRI BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 531 GCGG CCGC(-3/-1) AcsI 1253 AAATTC [GA]^AATT[CT] AcyI 505 GGCGTC G[GA]^CG[CT]C AfaI 548 GTAC GT^AC AgeI 177 ACCGGT A^CCGGT ApaI 290 GGGCCC GGGCC^C ApoI 1253 AAATTC [GA]^AATT[CT] AvaII 469 GGACC G^G[AT]CC BamHI 270 GGATCC G^GATCC BanI 857 GGTGCC G^G[CT][GA]CC BanII 290 GGGCCC G[GA]GC[CT]^C BcnI 267 CCCGG CC^[GC]GG BglI 1167 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 290 GGGCCC G[AGT]GC[ACT]^C BsaHI 505 GGCGTC G[GA]^CG[CT]C BsaI 484 GAGACC GGTCTC(1/5) BsgI 1258 CTGCAC GTGCAG(16/14) BsmBI 190 GAGACG CGTCTC(1/5) BsmI 1318 GAATGC GAATGC(1/-1) Bsp1286I 290 GGGCCC G[AGT]GC[ACT]^C BsrFI 177 ACCGGT [GA]^CCGG[CT] BstBI 282 TTCGAA TT^CGAA BstEII 580 GGTGACC G^GT[ACGT]ACC BstPI 580 GGTGACC G^GT[ACGT]ACC CfoI 172 GCGC GCG^C Cfr10I 177 ACCGGT [GA]^CCGG[CT] DraII 289 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 139 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 289 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 580 GGTGACC G^GT[ACGT]ACC FauI 531 GCGGG CCCGC(4/6) HaeII 171 GGCGCT [GA]GCGC^[CT] HhaI 172 GCGC GCG^C Hin1I 505 GGCGTC G[GA]^CG[CT]C HinP1I 172 GCGC G^CGC HindIII 673 AAGCTT A^AGCTT MaeII 632 ACGT A^CGT MseI 973 TTAA T^TAA NciI 267 CCCGG CC^[GC]GG NspI 600 GCATGT [GA]CATG^[CT] NspV 282 TTCGAA TT^CGAA PinAI 177 ACCGGT A^CCGGT RsaI 548 GTAC GT^AC SbfI 210 CCTGCAGG CCTGCA^GG SfuI 282 TTCGAA TT^CGAA Sse8387I 210 CCTGCAGG CCTGCA^GG SspI 901 AATATT AAT^ATT Tru9I 973 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbsI 433 GAAGAC GAAGAC(2/6) 1245 GAAGAC BbvCI 1087 CCTCAGC CCTCAGC(-5/-2) 219 GCTGAGG BciVI 614 GTATCC GTATCC(6/5) 1083 GTATCC BfaI 757 CTAG C^TAG 1136 CTAG BpuAI 433 GAAGAC GAAGAC(2/6) 1245 GAAGAC BsmAI 190 GAGAC GTCTC(1/5) 484 GAGAC BsmFI 468 GGGAC GGGAC(10/14) 245 GTCCC BsrDI 746 CATTGC GCAATG(2/0) 957 CATTGC BsrI 639 CCAGT ACTGG(1/-1) 1270 CCAGT BstAPI 204 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1186 GCAGAATCTGC Cac8I 950 GCTGGC GC[ACGT]^[ACGT]GC 961 GCCTGC EarI 1214 CTCTTC CTCTTC(1/4) 285 GAAGAG HaeIII 291 GGCC GG^CC 953 GGCC HgaI 192 GACGC GACGC(5/10) 506 GCGTC Ksp632I 1214 CTCTTC CTCTTC(1/4) 285 GAAGAG MaeI 757 CTAG C^TAG 1136 CTAG NcoI 585 CCATGG C^CATGG 1240 CCATGG PstI 211 CTGCAG CTGCA^G 1193 CTGCAG SmlI 325 CTTGAG C^T[CT][GA]AG 832 CTTGAG XcmI 861 CCAAATACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1107 CCAATGGAAAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AluI AlwI AlwNI BbvI BpmI BsaJI BsaWI BsiYI BslI BspMI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII