SLC47A2 ORF clone pFN21AB9096

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9096

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Clone info.

Species

human

Clone Name

pFN21AB9096 Sequence (with vector) Restriction Map

Product ID

FHC25932

ORF Sequence

DNA sequence (1806 bp)
Protein sequence (602 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB9096

Symbol

SLC47A2

Alias : MATE2, MATE2-B, MATE2-K, MATE2K

Description

solute carrier family 47 (multidrug and toxin extrusion), member 2, transcript variant 1

Original Clone ID

cp02285

Note

We obtained this clone using the E.coli strain JM109.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1806 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 602 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11211.1 0 100.0 SLC47A2 CCDS58530.1 3.3e-108 91.9 SLC47A2 CCDS11209.1 1.7e-70 48.3 SLC47A1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002528 40 181 PF01554 Multi antimicrobial extrusion protein IPR002528 297 458 PF01554 Multi antimicrobial extrusion protein HMMTigr IPR002528 213 471 TIGR00797 Multi antimicrobial extrusion protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 34 LFALSGPLFLFQVLTFMIYIVST 56 PRIMARY 23 2 67 LASVTLAVAFVNVCGVSVGVGLS 89 PRIMARY 23 3 108 VGVILQRGALVLLLCCLPCWALF 130 PRIMARY 23 4 150 TQDYVMIFIPGLPVIFLYNLLAK 172 PRIMARY 23 5 224 VVGNCVNGVANYALVSVLNLGVR 246 SECONDARY 23 6 249 AYANIISQFAQTVFLLLYIVLK 270 PRIMARY 22 7 285 LQDWGPFFSLAVPSMLMICVEWW 307 SECONDARY 23 8 315 LMGLLSVVDLSAQAVIYEVATVT 337 PRIMARY 23 9 341 PLGLSIGVCVRVGMALGAADTVQ 363 SECONDARY 23 10 368 AVSGVLSIVGISLVLGTLISILK 390 PRIMARY 23 11 403 VIALVSQVLPVYSVFHVFEAICC 425 SECONDARY 23 12 440 GAAVNAITYYIIGLPLGILLTFV 462 PRIMARY 23 13 470 LWLGMLACVFLATAAFVAYTARL 492 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_690872 602 609833 100.0 100.0 NP_001093116 566 609833 94.0 100.0 XP_016879710 616 609833 97.7 100.0 XP_016879711 567 609833 97.5 91.9 NP_001243592 580 609833 91.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9096 Download

>KIBB9096  1806 bp
ATGGACAGCCTCCAGGACACAGTGGCCCTGGACCATGGGGGCTGCTGCCCTGCCCTCAGC
AGGCTGGTTCCCAGAGGCTTTGGGACTGAGATGTGGACTCTCTTTGCCCTTTCTGGACCC
CTGTTCCTGTTCCAGGTGCTGACTTTTATGATCTACATCGTGAGCACTGTGTTCTGCGGG
CACCTGGGCAAGGTGGAGCTGGCATCGGTGACCCTCGCGGTGGCCTTTGTCAATGTCTGC
GGAGTTTCTGTAGGAGTTGGTTTGTCTTCGGCATGTGACACCTTGATGTCTCAGAGCTTC
GGCAGCCCCAACAAGAAGCACGTGGGCGTGATCCTGCAGCGGGGCGCGCTGGTCCTGCTC
CTCTGCTGCCTCCCTTGCTGGGCGCTCTTCCTCAACACCCAGCACATCCTGCTGCTCTTC
CGGCAGGACCCGGACGTGTCCAGGTTGACCCAGGACTATGTAATGATTTTCATTCCAGGA
CTTCCGGTGATTTTTCTTTACAATCTGCTGGCAAAATATTTGCAAAATCAGGGATGGCTG
AAGGGGCAGGAGGAGGAGTCCCCATTCCAAACCCCGGGTTTGTCCATCCTCCATCCATCT
CACTCACACCTTAGCAGGGCCAGTTTTCATTTATTTCAGAAGATCACCTGGCCCCAAGTC
CTCAGTGGTGTGGTGGGCAACTGTGTCAACGGTGTGGCCAACTATGCCCTGGTTTCTGTG
CTGAACCTGGGGGTCAGGGGCTCCGCCTATGCCAACATCATCTCCCAGTTTGCACAGACC
GTCTTCCTCCTTCTCTACATTGTGCTGAAGAAGCTGCACCTGGAGACGTGGGCAGGTTGG
TCCAGCCAGTGCCTGCAGGACTGGGGCCCCTTCTTCTCCCTGGCTGTCCCCAGCATGCTC
ATGATCTGTGTTGAGTGGTGGGCCTATGAGATCGGGAGCTTCCTCATGGGGCTGCTCAGT
GTGGTGGATCTCTCTGCCCAGGCTGTCATCTATGAGGTGGCCACTGTGACCTACATGATT
CCCTTGGGGCTCAGCATCGGGGTCTGTGTCCGAGTGGGGATGGCTCTGGGGGCTGCGGAT
ACTGTGCAGGCCAAGCGCTCGGCCGTCTCGGGCGTGCTCAGCATAGTTGGCATTTCCCTG
GTCCTGGGCACCCTGATAAGCATCCTGAAAAATCAGCTGGGGCATATTTTTACCAATGAT
GAAGATGTCATTGCCCTGGTGAGCCAGGTCTTGCCGGTTTATAGTGTCTTTCACGTGTTT
GAGGCCATCTGTTGTGTCTATGGCGGAGTTCTGAGAGGAACTGGGAAGCAGGCCTTTGGT
GCCGCTGTGAATGCCATCACATATTACATCATCGGCCTACCACTGGGCATCCTTCTGACC
TTTGTGGTCAGAATGAGAATCATGGGCCTCTGGCTGGGCATGCTGGCCTGTGTCTTCCTG
GCAACTGCTGCCTTTGTTGCTTATACTGCCCGGCTGGACTGGAAGCTTGCTGCAGAGGAG
GCTAAGAAACATTCAGGCCGGCAGCAGCAGCAGAGAGCAGAGAGCACTGCAACCAGACCT
GGGCCTGAGAAAGCAGTCCTATCTTCAGTGGCTACAGGCAGTTCCCCTGGCATTACCTTG
ACAACGTATTCAAGGTCTGAGTGCCACGTGGACTTCTTCAGGACTCCAGAGGAGGCCCAC
GCCCTTTCAGCTCCTACCAGCAGACTATCAGTGAAACAGCTGGTCATCCGCCGTGGGGCT
GCTCTGGGGGCGGCGTCAGCCACACTGATGGTGGGGCTCACGGTCAGGATCCTAGCCACC
AGGCAC

ORF Protein

Cloned ORF protein sequence for pFN21AB9096 Download

>KIBB9096  602 aa
MDSLQDTVALDHGGCCPALSRLVPRGFGTEMWTLFALSGPLFLFQVLTFMIYIVSTVFCG
HLGKVELASVTLAVAFVNVCGVSVGVGLSSACDTLMSQSFGSPNKKHVGVILQRGALVLL
LCCLPCWALFLNTQHILLLFRQDPDVSRLTQDYVMIFIPGLPVIFLYNLLAKYLQNQGWL
KGQEEESPFQTPGLSILHPSHSHLSRASFHLFQKITWPQVLSGVVGNCVNGVANYALVSV
LNLGVRGSAYANIISQFAQTVFLLLYIVLKKLHLETWAGWSSQCLQDWGPFFSLAVPSML
MICVEWWAYEIGSFLMGLLSVVDLSAQAVIYEVATVTYMIPLGLSIGVCVRVGMALGAAD
TVQAKRSAVSGVLSIVGISLVLGTLISILKNQLGHIFTNDEDVIALVSQVLPVYSVFHVF
EAICCVYGGVLRGTGKQAFGAAVNAITYYIIGLPLGILLTFVVRMRIMGLWLGMLACVFL
ATAAFVAYTARLDWKLAAEEAKKHSGRQQQQRAESTATRPGPEKAVLSSVATGSSPGITL
TTYSRSECHVDFFRTPEEAHALSAPTSRLSVKQLVIRRGAALGAASATLMVGLTVRILAT
RH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9096 Download

>pFN21AB9096  6511 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACAGCCTCCAGGACACAGTGGCCCTGGACCA
TGGGGGCTGCTGCCCTGCCCTCAGCAGGCTGGTTCCCAGAGGCTTTGGGACTGAGATGTG
GACTCTCTTTGCCCTTTCTGGACCCCTGTTCCTGTTCCAGGTGCTGACTTTTATGATCTA
CATCGTGAGCACTGTGTTCTGCGGGCACCTGGGCAAGGTGGAGCTGGCATCGGTGACCCT
CGCGGTGGCCTTTGTCAATGTCTGCGGAGTTTCTGTAGGAGTTGGTTTGTCTTCGGCATG
TGACACCTTGATGTCTCAGAGCTTCGGCAGCCCCAACAAGAAGCACGTGGGCGTGATCCT
GCAGCGGGGCGCGCTGGTCCTGCTCCTCTGCTGCCTCCCTTGCTGGGCGCTCTTCCTCAA
CACCCAGCACATCCTGCTGCTCTTCCGGCAGGACCCGGACGTGTCCAGGTTGACCCAGGA
CTATGTAATGATTTTCATTCCAGGACTTCCGGTGATTTTTCTTTACAATCTGCTGGCAAA
ATATTTGCAAAATCAGGGATGGCTGAAGGGGCAGGAGGAGGAGTCCCCATTCCAAACCCC
GGGTTTGTCCATCCTCCATCCATCTCACTCACACCTTAGCAGGGCCAGTTTTCATTTATT
TCAGAAGATCACCTGGCCCCAAGTCCTCAGTGGTGTGGTGGGCAACTGTGTCAACGGTGT
GGCCAACTATGCCCTGGTTTCTGTGCTGAACCTGGGGGTCAGGGGCTCCGCCTATGCCAA
CATCATCTCCCAGTTTGCACAGACCGTCTTCCTCCTTCTCTACATTGTGCTGAAGAAGCT
GCACCTGGAGACGTGGGCAGGTTGGTCCAGCCAGTGCCTGCAGGACTGGGGCCCCTTCTT
CTCCCTGGCTGTCCCCAGCATGCTCATGATCTGTGTTGAGTGGTGGGCCTATGAGATCGG
GAGCTTCCTCATGGGGCTGCTCAGTGTGGTGGATCTCTCTGCCCAGGCTGTCATCTATGA
GGTGGCCACTGTGACCTACATGATTCCCTTGGGGCTCAGCATCGGGGTCTGTGTCCGAGT
GGGGATGGCTCTGGGGGCTGCGGATACTGTGCAGGCCAAGCGCTCGGCCGTCTCGGGCGT
GCTCAGCATAGTTGGCATTTCCCTGGTCCTGGGCACCCTGATAAGCATCCTGAAAAATCA
GCTGGGGCATATTTTTACCAATGATGAAGATGTCATTGCCCTGGTGAGCCAGGTCTTGCC
GGTTTATAGTGTCTTTCACGTGTTTGAGGCCATCTGTTGTGTCTATGGCGGAGTTCTGAG
AGGAACTGGGAAGCAGGCCTTTGGTGCCGCTGTGAATGCCATCACATATTACATCATCGG
CCTACCACTGGGCATCCTTCTGACCTTTGTGGTCAGAATGAGAATCATGGGCCTCTGGCT
GGGCATGCTGGCCTGTGTCTTCCTGGCAACTGCTGCCTTTGTTGCTTATACTGCCCGGCT
GGACTGGAAGCTTGCTGCAGAGGAGGCTAAGAAACATTCAGGCCGGCAGCAGCAGCAGAG
AGCAGAGAGCACTGCAACCAGACCTGGGCCTGAGAAAGCAGTCCTATCTTCAGTGGCTAC
AGGCAGTTCCCCTGGCATTACCTTGACAACGTATTCAAGGTCTGAGTGCCACGTGGACTT
CTTCAGGACTCCAGAGGAGGCCCACGCCCTTTCAGCTCCTACCAGCAGACTATCAGTGAA
ACAGCTGGTCATCCGCCGTGGGGCTGCTCTGGGGGCGGCGTCAGCCACACTGATGGTGGG
GCTCACGGTCAGGATCCTAGCCACCAGGCACGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT